目的:探讨益气养阴方辅助治疗对2型糖尿病患者糖脂代谢与外周血apelin、FABP4表达的影响。方法:将就诊的2型糖尿病患者按照随机数字表随机纳入,每组124例,对照组给予磷酸西格列汀片。观察组,在对照组基础上,结合益气养阴方治疗,观察两...目的:探讨益气养阴方辅助治疗对2型糖尿病患者糖脂代谢与外周血apelin、FABP4表达的影响。方法:将就诊的2型糖尿病患者按照随机数字表随机纳入,每组124例,对照组给予磷酸西格列汀片。观察组,在对照组基础上,结合益气养阴方治疗,观察两组治疗前及治疗后HOMA-IR、HOMA-β指标变化,血清nesfatin-1、apelin及HbA1c、FBG及2 h PG,患者临床症状,FABP4、PPARγ比较。结果:治疗后,观察组有效率为71.77%明显优于对照组的54.83%(P<0.05);同时,治疗后观察组各项症状评分优于对照组(P<0.05);治疗后观察组HOMA-β、HOMA-IR、nesfatin-1、apelin、FABP4、PPARγ与对照组存在显著差异(P<0.05)。用药24周后观察组FBG、2 h PG、HbAlc、FINS低于对照组(P<0.05)。结论:益气养阴方辅助治疗有利于改善2型糖尿病患者糖脂代谢情况,下调患者外周血中参与糖及脂肪代谢及胰岛素抵抗相关基因的apelin、FABP4表达水平,减少患者出现胰岛素抵抗的可能,并能改善患者高血糖相关症状,从而具有一定临床应用价值。展开更多
This paper aims to observe the changes of the inflammatory cytokines in microglial BV2 cells stimulated by apelin, and inves- tigate the mechanism of inflammatory cytokines secretion after apelin stimulation. Immunofl...This paper aims to observe the changes of the inflammatory cytokines in microglial BV2 cells stimulated by apelin, and inves- tigate the mechanism of inflammatory cytokines secretion after apelin stimulation. Immunofluorescence and quantitative re- al-time PCR were performed to observe expression of TNF-α, IL-Iβ, IL-10, MIP-let, and MCP-1 in BV2 cells. Western blot was used to investigate the expression of phosphorylation PI-3K/Akt and phosphorylation Erk signaling pathways in BV2 cells after stimulation by apelin. Furthermore, PI-3K/Akt inhibitor （LY294402） and Erk inhibitor （U0126） were used as antagonists to detect the secretion mechanisms of cytokines in BV2 cells stimulated by apelin. Exogenous recombinant apelin activated the expression of TNF-α, IL-1β, MCP-1 and MIP-1α in BV2 cells by the detection of fluorescence expression and mRNA. Apelin also unregulated the protein expression of p-PI-3K/Akt and p-Erk in BV2 cells induced by apelin. LY294002 and U0126 in- hibited activation of p-PI-3K/Akt and p-Erk expression by Western blot and attenuated the expression of inflammation factors in BV2 cells by fluorescence staining. This study demonstrates that apelin is a potential activator of inflammation factors through the PI3K/Akt and Erk signaling pathway and is potential therapeutically relevant to inflammatory responses of micro- glia cells.展开更多
文摘目的:探讨益气养阴方辅助治疗对2型糖尿病患者糖脂代谢与外周血apelin、FABP4表达的影响。方法:将就诊的2型糖尿病患者按照随机数字表随机纳入,每组124例,对照组给予磷酸西格列汀片。观察组,在对照组基础上,结合益气养阴方治疗,观察两组治疗前及治疗后HOMA-IR、HOMA-β指标变化,血清nesfatin-1、apelin及HbA1c、FBG及2 h PG,患者临床症状,FABP4、PPARγ比较。结果:治疗后,观察组有效率为71.77%明显优于对照组的54.83%(P<0.05);同时,治疗后观察组各项症状评分优于对照组(P<0.05);治疗后观察组HOMA-β、HOMA-IR、nesfatin-1、apelin、FABP4、PPARγ与对照组存在显著差异(P<0.05)。用药24周后观察组FBG、2 h PG、HbAlc、FINS低于对照组(P<0.05)。结论:益气养阴方辅助治疗有利于改善2型糖尿病患者糖脂代谢情况,下调患者外周血中参与糖及脂肪代谢及胰岛素抵抗相关基因的apelin、FABP4表达水平,减少患者出现胰岛素抵抗的可能,并能改善患者高血糖相关症状,从而具有一定临床应用价值。
基金supported by European Foundation for the Study of Diabetes/Chinese Diabetes Society/Lilly grant(90561 and 94410)
文摘This paper aims to observe the changes of the inflammatory cytokines in microglial BV2 cells stimulated by apelin, and inves- tigate the mechanism of inflammatory cytokines secretion after apelin stimulation. Immunofluorescence and quantitative re- al-time PCR were performed to observe expression of TNF-α, IL-Iβ, IL-10, MIP-let, and MCP-1 in BV2 cells. Western blot was used to investigate the expression of phosphorylation PI-3K/Akt and phosphorylation Erk signaling pathways in BV2 cells after stimulation by apelin. Furthermore, PI-3K/Akt inhibitor （LY294402） and Erk inhibitor （U0126） were used as antagonists to detect the secretion mechanisms of cytokines in BV2 cells stimulated by apelin. Exogenous recombinant apelin activated the expression of TNF-α, IL-1β, MCP-1 and MIP-1α in BV2 cells by the detection of fluorescence expression and mRNA. Apelin also unregulated the protein expression of p-PI-3K/Akt and p-Erk in BV2 cells induced by apelin. LY294002 and U0126 in- hibited activation of p-PI-3K/Akt and p-Erk expression by Western blot and attenuated the expression of inflammation factors in BV2 cells by fluorescence staining. This study demonstrates that apelin is a potential activator of inflammation factors through the PI3K/Akt and Erk signaling pathway and is potential therapeutically relevant to inflammatory responses of micro- glia cells.
