Aphid is one of the most destructive insect pests on cultivated plants in temperate regions.Their piercing-sucking mouthparts and phloem feeding behavior directly damage crops and deplete plant nutrients.Potato(Solanu...Aphid is one of the most destructive insect pests on cultivated plants in temperate regions.Their piercing-sucking mouthparts and phloem feeding behavior directly damage crops and deplete plant nutrients.Potato(Solanum tuberosum L.)is one of the most important food sources on the planet,and several aphid species,e.g.,Myzus persicae(Sulzer)(green peach aphid)and Macrosiphum euphorbiae(Thomas)(potato aphid)(Hemiptera:Aphididae)colonize potato and transmit several economically important viruses.Aphid-transmitted potato viruses have been emerging all over the world as a very serious problem in potato production,inducing a wide variety of foliar and tuber symptoms,leading to severe yield reduction and loss of tuber quality.In this review,recent advances in understanding the interactions of potato viruses with their hosts,aphid vectors and the environment are described.展开更多
Using 2-D electrophoresis and virus overlay assay, a 50-kDa protein (P50) exhibiting specific binding to purified virus particles of BYDV-GAV was found in the protein extracts from Schizaphis graminum and Sitobion ave...Using 2-D electrophoresis and virus overlay assay, a 50-kDa protein (P50) exhibiting specific binding to purified virus particles of BYDV-GAV was found in the protein extracts from Schizaphis graminum and Sitobion avenae, two aphid species transmitting BYDV-GAV. P50 in the extracts of S. graminum was isolated by preparation electrophoresis and electro-eluted proteins from the gel slices for antiserum preparation. After feeding the antiserum through membrane, the transmission efficiencies of S. graminum and S. avenae for BYDV-GAV decreased significantly. It was suggested that P50 should be related with transmission pro- cess. Location of P50 was found at the plasma membrane surrounding the accessory salivary gland (ASG) in the head tissues of S. graminum by immunogold-labelling experiment. The ascertainment of the protein associated with virus transmission has a significance influence on further understanding the transmission mechanism and genetic engineering for resistant to vector transmission.展开更多
对4种柑橘衰退病毒源及其31个单蚜传毒分离株的CP基因做了限制性片段长度多态性(RFLP)和单链构象多态性(SSCP)分析,并对其中8个单蚜传毒分离株的CP基因进行了序列分析。实验明确了所研究柑橘衰退病毒(Citrus triste-za virus,CTV)的CPG/...对4种柑橘衰退病毒源及其31个单蚜传毒分离株的CP基因做了限制性片段长度多态性(RFLP)和单链构象多态性(SSCP)分析,并对其中8个单蚜传毒分离株的CP基因进行了序列分析。实验明确了所研究柑橘衰退病毒(Citrus triste-za virus,CTV)的CPG/Hinf I RFLP组群和CPG/SSCP模式,二者能较好地对应并有效验证了单蚜传毒对CTV毒源的分离纯化作用;CP基因序列分析结果表明,相同CPG/Hinf I RFLP组群的单蚜传毒分离株间具有高度的序列相似性,而不同CPG/Hinf I RFLP组群单蚜传毒分离株间则存在较大差异;通过与已知生物学特性CTV分离株比较,初步建立了上述CP基因分子特征与病毒生物学特性之间的联系。展开更多
基金supported by the grants from the Startup Fund for Distinguished Scholars, Nanjing Agricultural University, China
文摘Aphid is one of the most destructive insect pests on cultivated plants in temperate regions.Their piercing-sucking mouthparts and phloem feeding behavior directly damage crops and deplete plant nutrients.Potato(Solanum tuberosum L.)is one of the most important food sources on the planet,and several aphid species,e.g.,Myzus persicae(Sulzer)(green peach aphid)and Macrosiphum euphorbiae(Thomas)(potato aphid)(Hemiptera:Aphididae)colonize potato and transmit several economically important viruses.Aphid-transmitted potato viruses have been emerging all over the world as a very serious problem in potato production,inducing a wide variety of foliar and tuber symptoms,leading to severe yield reduction and loss of tuber quality.In this review,recent advances in understanding the interactions of potato viruses with their hosts,aphid vectors and the environment are described.
基金This work was supported by the National Key Basic Research of China(Grant No.TG2000016201)the National Natural Science Foundation of China(Grant No.30070498).
文摘Using 2-D electrophoresis and virus overlay assay, a 50-kDa protein (P50) exhibiting specific binding to purified virus particles of BYDV-GAV was found in the protein extracts from Schizaphis graminum and Sitobion avenae, two aphid species transmitting BYDV-GAV. P50 in the extracts of S. graminum was isolated by preparation electrophoresis and electro-eluted proteins from the gel slices for antiserum preparation. After feeding the antiserum through membrane, the transmission efficiencies of S. graminum and S. avenae for BYDV-GAV decreased significantly. It was suggested that P50 should be related with transmission pro- cess. Location of P50 was found at the plasma membrane surrounding the accessory salivary gland (ASG) in the head tissues of S. graminum by immunogold-labelling experiment. The ascertainment of the protein associated with virus transmission has a significance influence on further understanding the transmission mechanism and genetic engineering for resistant to vector transmission.
文摘对4种柑橘衰退病毒源及其31个单蚜传毒分离株的CP基因做了限制性片段长度多态性(RFLP)和单链构象多态性(SSCP)分析,并对其中8个单蚜传毒分离株的CP基因进行了序列分析。实验明确了所研究柑橘衰退病毒(Citrus triste-za virus,CTV)的CPG/Hinf I RFLP组群和CPG/SSCP模式,二者能较好地对应并有效验证了单蚜传毒对CTV毒源的分离纯化作用;CP基因序列分析结果表明,相同CPG/Hinf I RFLP组群的单蚜传毒分离株间具有高度的序列相似性,而不同CPG/Hinf I RFLP组群单蚜传毒分离株间则存在较大差异;通过与已知生物学特性CTV分离株比较,初步建立了上述CP基因分子特征与病毒生物学特性之间的联系。
