A new Sillago species,Sillago parasihama sp.n.,is identified based on 127 specimens collected from the southern coast of China.We compared the morphological characters between Sillago parasihama and all other 11 Silla...A new Sillago species,Sillago parasihama sp.n.,is identified based on 127 specimens collected from the southern coast of China.We compared the morphological characters between Sillago parasihama and all other 11 Sillago species with two posterior extensions on the swim bladder.The new species is like S.sihama in the countable characters and color pattern,but is different from the latter by the distinct swim bladders.The swim bladder of S.parasihama is without lateral process.The posterior sub-extensions of anterolateral extensions are unique with some dendritic or sometimes stunted blind tubule,which are unilateral and outward,ex-tending along the abdominal,and are about one-third to half of the body of swim bladder in length.But the swim bladder of S.si-hama with 8-10 lateral processes,the posterior sub-extensions of anterolateral extensions are kinky,long and complicated,extend-ing along the abdominal wall below the peritoneum to the base of posterior extensions.S.parasihama can be distinguished from other species in this group by color pattern,meristic,and morphometric characters.Moreover,the results of genetic analysis using sequences of the mitochondrial cytochrome c oxidase subunit I(COI)fragment show significant interspecific-level genetic distances(0.159-0.231)between S.parasihama and 8 congeners in the group,which also support the validity of new species.We also provide a distribution map and a key of the related species.展开更多
Using a standard cytochrome c oxidase I sequence,DNA barcoding has been shown to be effective to distinguish known species and to discover cryptic species. Here we assessed the efficiency of DNA barcoding for the amph...Using a standard cytochrome c oxidase I sequence,DNA barcoding has been shown to be effective to distinguish known species and to discover cryptic species. Here we assessed the efficiency of DNA barcoding for the amphipod genus Gammarus from China. The maximum intraspecific divergence for widespread species,Gammarus lacustris,was 3.5%,and mean interspecific divergence reached 21.9%. We presented a conservative benchmark for determining provisional species using maximum intraspecific divergence of Gammarus lacustris. Thirty-one species possessed distinct barcode clusters. Two species were comprised of highly divergent clades with strong neighbor-joining bootstrap values,and likely indicated the presence of cryptic species. Although DNA barcoding is effective,future identification of species of Gammarus should incorporate DNA barcoding and morphological detection.展开更多
The introduction of invasive insect pests across national borders has become a major concern in crop production. Accordingly, national plant protection organizations are challenge to reinforce their monitoring strateg...The introduction of invasive insect pests across national borders has become a major concern in crop production. Accordingly, national plant protection organizations are challenge to reinforce their monitoring strategies, which are hampered by the weight and size of inspection equipment, as well as the taxonomic extensiveness of interrupted species. Moreover, some insect pests that impede farmer productivity and profitability are difficult for researchers to address on time due to a lack of appropriate plant protection measures. Farmers’ reliance on synthetic pesticides and biocontrol agents has resulted in major economic and environmental ramifications. DNA barcoding is a novel technology that has the potential to improve Integrated Pest Management decision-making, which is dependent on the ability to correctly identify pest and beneficial organisms. This is due to some natural traits such as phenology or pesticide susceptibility browbeaten by IPM strategies to avert pest establishment. Specifically, Deoxyribonucleic acid (DNA) sequence information was applied effectively for the identification of some micro-organisms. This technology, DNA barcoding, allows for the identification of insect species by using short, standardized gene sequences. DNA barcoding is basically based on repeatable and accessible technique that allows for the mechanisation or automation of species discrimination. This technique bridges the taxonomic bio-security gap and meets the International Plant Protection Convention diagnostic standards for insect identification. This review therefore discusses DNA barcoding as a technique for insect pests’ identification and its potential application for crop protection.展开更多
为了探讨DNA条形码技术在夜蛾物种鉴定中的可行性,本研究利用条形码通用引物扩增了北京百花山地区43种夜蛾75个样本的线粒体细胞色素C氧化酶亚基I(mitochondrial cytochrome c oxidase subunitI,COI)基因序列,以Kimura双参数模型进行种...为了探讨DNA条形码技术在夜蛾物种鉴定中的可行性,本研究利用条形码通用引物扩增了北京百花山地区43种夜蛾75个样本的线粒体细胞色素C氧化酶亚基I(mitochondrial cytochrome c oxidase subunitI,COI)基因序列,以Kimura双参数模型进行种内种间遗传距离分析、使用邻接法(neighbor-joining,NJ)和最大简约法(maximumparsimony,MP)分别构建系统发育树,并利用分子序列差异阈值对样本进行分子可操作分类单元(molecular defined operational taxonomic units,MOTU)划分。结果表明:所有夜蛾种类通过系统发育树可以成功区分;种内平均遗传距离(0.03%)远远小于种间平均遗传距离(11.29%);采用较为保守的1%的序列差异阈值将75个夜蛾样本分为42个MOTU,正确率为95%,除了MOTU04包含2个物种外,剩余41个MOTU与形态种呈现一一对应的关系。结果显示,基于COI基因的DNA条形码对于本研究中所涉及的夜蛾具有较好的区分,可以作为一种有效的工具在夜蛾科昆虫物种鉴定中进行应用。展开更多
基金supported by the National Key Research and Development Program of China(No.2019 YFD0901301)the National Natural Science Foundation of China(Nos.41976083,41776171).
文摘A new Sillago species,Sillago parasihama sp.n.,is identified based on 127 specimens collected from the southern coast of China.We compared the morphological characters between Sillago parasihama and all other 11 Sillago species with two posterior extensions on the swim bladder.The new species is like S.sihama in the countable characters and color pattern,but is different from the latter by the distinct swim bladders.The swim bladder of S.parasihama is without lateral process.The posterior sub-extensions of anterolateral extensions are unique with some dendritic or sometimes stunted blind tubule,which are unilateral and outward,ex-tending along the abdominal,and are about one-third to half of the body of swim bladder in length.But the swim bladder of S.si-hama with 8-10 lateral processes,the posterior sub-extensions of anterolateral extensions are kinky,long and complicated,extend-ing along the abdominal wall below the peritoneum to the base of posterior extensions.S.parasihama can be distinguished from other species in this group by color pattern,meristic,and morphometric characters.Moreover,the results of genetic analysis using sequences of the mitochondrial cytochrome c oxidase subunit I(COI)fragment show significant interspecific-level genetic distances(0.159-0.231)between S.parasihama and 8 congeners in the group,which also support the validity of new species.We also provide a distribution map and a key of the related species.
基金supported by the National Natural Sciences Foundation of China (NSFC-30499341/30770268/30870473)Beijing Financial Budget Index (2008-NO.0178)the Beijing Natural Science Foundation (5082013)
文摘Using a standard cytochrome c oxidase I sequence,DNA barcoding has been shown to be effective to distinguish known species and to discover cryptic species. Here we assessed the efficiency of DNA barcoding for the amphipod genus Gammarus from China. The maximum intraspecific divergence for widespread species,Gammarus lacustris,was 3.5%,and mean interspecific divergence reached 21.9%. We presented a conservative benchmark for determining provisional species using maximum intraspecific divergence of Gammarus lacustris. Thirty-one species possessed distinct barcode clusters. Two species were comprised of highly divergent clades with strong neighbor-joining bootstrap values,and likely indicated the presence of cryptic species. Although DNA barcoding is effective,future identification of species of Gammarus should incorporate DNA barcoding and morphological detection.
文摘The introduction of invasive insect pests across national borders has become a major concern in crop production. Accordingly, national plant protection organizations are challenge to reinforce their monitoring strategies, which are hampered by the weight and size of inspection equipment, as well as the taxonomic extensiveness of interrupted species. Moreover, some insect pests that impede farmer productivity and profitability are difficult for researchers to address on time due to a lack of appropriate plant protection measures. Farmers’ reliance on synthetic pesticides and biocontrol agents has resulted in major economic and environmental ramifications. DNA barcoding is a novel technology that has the potential to improve Integrated Pest Management decision-making, which is dependent on the ability to correctly identify pest and beneficial organisms. This is due to some natural traits such as phenology or pesticide susceptibility browbeaten by IPM strategies to avert pest establishment. Specifically, Deoxyribonucleic acid (DNA) sequence information was applied effectively for the identification of some micro-organisms. This technology, DNA barcoding, allows for the identification of insect species by using short, standardized gene sequences. DNA barcoding is basically based on repeatable and accessible technique that allows for the mechanisation or automation of species discrimination. This technique bridges the taxonomic bio-security gap and meets the International Plant Protection Convention diagnostic standards for insect identification. This review therefore discusses DNA barcoding as a technique for insect pests’ identification and its potential application for crop protection.
文摘为了探讨DNA条形码技术在夜蛾物种鉴定中的可行性,本研究利用条形码通用引物扩增了北京百花山地区43种夜蛾75个样本的线粒体细胞色素C氧化酶亚基I(mitochondrial cytochrome c oxidase subunitI,COI)基因序列,以Kimura双参数模型进行种内种间遗传距离分析、使用邻接法(neighbor-joining,NJ)和最大简约法(maximumparsimony,MP)分别构建系统发育树,并利用分子序列差异阈值对样本进行分子可操作分类单元(molecular defined operational taxonomic units,MOTU)划分。结果表明:所有夜蛾种类通过系统发育树可以成功区分;种内平均遗传距离(0.03%)远远小于种间平均遗传距离(11.29%);采用较为保守的1%的序列差异阈值将75个夜蛾样本分为42个MOTU,正确率为95%,除了MOTU04包含2个物种外,剩余41个MOTU与形态种呈现一一对应的关系。结果显示,基于COI基因的DNA条形码对于本研究中所涉及的夜蛾具有较好的区分,可以作为一种有效的工具在夜蛾科昆虫物种鉴定中进行应用。
