To evaluate the action of Apium graveolens L. on the growth and differentiation of neural stem cells （NSCs）, cells culture and animal experiment were performed. NSCs were isolated from the striatum of SD rat embryo ...To evaluate the action of Apium graveolens L. on the growth and differentiation of neural stem cells （NSCs）, cells culture and animal experiment were performed. NSCs were isolated from the striatum of SD rat embryo were cultured in the medium containing aqueous extract from Apium graveolens L（AAG）.and/or the serum derived from mice treated with Apium graveolens L （SAG）. The results show that AAG promoted the survival and growth of NSCs in low concentration. Apium graveolens L. leaves aqueous extract promoted the proliferation of NSCs in relatively high concentration. SAG significantly accelerated the differentiation of NSCs.展开更多
Inula graveolens L. is widely used in Iraq for the treatment of rheumatic fever, infant convulsions, toothache, blood sugar, and also to dissolve internal blood clots, and to aid digestion. However, the efficacy and m...Inula graveolens L. is widely used in Iraq for the treatment of rheumatic fever, infant convulsions, toothache, blood sugar, and also to dissolve internal blood clots, and to aid digestion. However, the efficacy and mechanisms of action of the plant remain unclear. Therefore, the objective of the present study was to investigate the pharmacological effects of the methanolic extract (MEIG) of this plant belonging to compositae family. Anti-diarrheal and antipyretic activities of the extract were examined in rats. Anti-inflammatory and antinociceptive were studied in mice. At the doses of 200 (P in vitro protein anti-denaturation using Bovine serum albumin and anti-platelet aggregation of human blood activity. It was observed that the extract showed greater percentage of inhibition of BSA (P potential platelet aggregation inhibitory activity in adose-dependent manner. The maximum inhibition was observed at the dose 400 μg/ml展开更多
The production of free radicals is a natural process in aerobic organisms due to the mitochondrial activity. Usually the cells have several mechanisms for scavenging those free radicals, but, in several pathologic pro...The production of free radicals is a natural process in aerobic organisms due to the mitochondrial activity. Usually the cells have several mechanisms for scavenging those free radicals, but, in several pathologic processes, such as cancer, those free radicals increase their production, making it impossible to sustain the system stable, generating the condition called oxidative stress. Ruta graveolens L. （Rue） is a plant commonly used in traditional medicine, mainly as antiinflamatory; this has been related to some organic components, such as Rutin, but there hasn＇t been any lectin studies in Rue stem. Lectins are glycoproteins of non-enzymatic and no immune origin, able to bind to simple carbohydrates, which lets them bind selectively to malignant cells against normal cells, killing them via apoptosis and reducing the free radicals level. In this study we intended to characterize rue stem lectins as those weren＇t reported yet. Also, the anticancer and antioxidant activity of these lectins was evaluated, Rue stem lectins were extracted using a saline solution and semipurified to obtain an enriched extract and administrated to nickel oxide treated mice. Oxidative stress was cuantified using the tiobarbituric acid （TBARS） method to quantify Malondialdehyde （MDA）, the Griess method to cuantify Nitrites and enzymatic activity of catalase were cuantified in liver. In this study was found that rue stem lectins are useful as a therapeutic auxiliar, considering that its ratio of antioxidant activity is limited, being a prooxidant agent at high concentrations.展开更多
A pot experiment and a sandy culture experiment grown with three vegetable crops of Chinese cabbage ( B. chinensis L., cv. Zao\|Shu 5), winter greens ( B.var.rosularis Tsen et Lee, cv. Shang\|Hai\|Qing) and cele...A pot experiment and a sandy culture experiment grown with three vegetable crops of Chinese cabbage ( B. chinensis L., cv. Zao\|Shu 5), winter greens ( B.var.rosularis Tsen et Lee, cv. Shang\|Hai\|Qing) and celery ( A.graveolens L. var. dulce DC., cv. Qing\|Qin) were conducted, respectively. The initial soil and four incubated soils with different extractable Cd (0.15, 0.89, 1.38, 1.84 and 2.30 mg Cd/kg soil) were used for the pot experiment. Five treatments were designed (0, 0.0625, 0.125, 0.250 and 0.500 mg Cd/L) in nutrient solution in the sandy culture experiment. Each treatment in pot and sandy culture experiments was trireplicated. The objectives of the study were to examine Cd accumulation in edible parts of selected vegetable crops, its correlation with Cd concentrations in vegetable garden soil or in nutrient solution, and evaluate the criteria of Cd pollution in vegetable garden soil and in nutrient solution based on the hygienic limit of Cd in vegetables. Cadmium concentrations in edible parts of the three selected vegetable crops were as follows: 0.01—0.15 mg/kg fresh weight for Chinese cabbage, 0.02—0.17 mg/kg fresh weight for winter greens, and 0.02—0.24 mg/kg fresh weight for celery in the pot experiment, and 0.1—0.4 mg/kg fresh weight for Chinese cabbage, 0.1—1.4 mg/kg fresh weight for winter greens, and 0.05—0.5 mg/kg fresh weight for celery in the pot experiment(except no\|Cd treatment). The order of the three test vegetable crops for cadmium accumulation in the edible parts was celery>winter greens>Chinese cabbage in both the pot experiment and the sandy culture experiment. Cadmium accumulation in edible parts or roots of the vegetable crops increased with increasing of cadmium concentration in the medium(soil or nutrient solution). And cadmium concentrations in edible parts of the test vegetable crops were significantly linearly related to the Cd levels in the growth media (soil and nutrient solution). Based on the regression equations established and the limit of cadmium concentration in vegetable products, the thresholds of Cd concentration in the growth medium evaluated was as follows: 0.5 mg/kg soil of extractable Cd for soil and 0.02 mg /L for nutrient solution. The high capacity for cadmium accumulation in the edible parts of different vegetable crops together with the absence of visual symptoms implies a potential danger for humans.展开更多
文摘To evaluate the action of Apium graveolens L. on the growth and differentiation of neural stem cells （NSCs）, cells culture and animal experiment were performed. NSCs were isolated from the striatum of SD rat embryo were cultured in the medium containing aqueous extract from Apium graveolens L（AAG）.and/or the serum derived from mice treated with Apium graveolens L （SAG）. The results show that AAG promoted the survival and growth of NSCs in low concentration. Apium graveolens L. leaves aqueous extract promoted the proliferation of NSCs in relatively high concentration. SAG significantly accelerated the differentiation of NSCs.
文摘Inula graveolens L. is widely used in Iraq for the treatment of rheumatic fever, infant convulsions, toothache, blood sugar, and also to dissolve internal blood clots, and to aid digestion. However, the efficacy and mechanisms of action of the plant remain unclear. Therefore, the objective of the present study was to investigate the pharmacological effects of the methanolic extract (MEIG) of this plant belonging to compositae family. Anti-diarrheal and antipyretic activities of the extract were examined in rats. Anti-inflammatory and antinociceptive were studied in mice. At the doses of 200 (P in vitro protein anti-denaturation using Bovine serum albumin and anti-platelet aggregation of human blood activity. It was observed that the extract showed greater percentage of inhibition of BSA (P potential platelet aggregation inhibitory activity in adose-dependent manner. The maximum inhibition was observed at the dose 400 μg/ml
文摘The production of free radicals is a natural process in aerobic organisms due to the mitochondrial activity. Usually the cells have several mechanisms for scavenging those free radicals, but, in several pathologic processes, such as cancer, those free radicals increase their production, making it impossible to sustain the system stable, generating the condition called oxidative stress. Ruta graveolens L. （Rue） is a plant commonly used in traditional medicine, mainly as antiinflamatory; this has been related to some organic components, such as Rutin, but there hasn＇t been any lectin studies in Rue stem. Lectins are glycoproteins of non-enzymatic and no immune origin, able to bind to simple carbohydrates, which lets them bind selectively to malignant cells against normal cells, killing them via apoptosis and reducing the free radicals level. In this study we intended to characterize rue stem lectins as those weren＇t reported yet. Also, the anticancer and antioxidant activity of these lectins was evaluated, Rue stem lectins were extracted using a saline solution and semipurified to obtain an enriched extract and administrated to nickel oxide treated mice. Oxidative stress was cuantified using the tiobarbituric acid （TBARS） method to quantify Malondialdehyde （MDA）, the Griess method to cuantify Nitrites and enzymatic activity of catalase were cuantified in liver. In this study was found that rue stem lectins are useful as a therapeutic auxiliar, considering that its ratio of antioxidant activity is limited, being a prooxidant agent at high concentrations.
文摘A pot experiment and a sandy culture experiment grown with three vegetable crops of Chinese cabbage ( B. chinensis L., cv. Zao\|Shu 5), winter greens ( B.var.rosularis Tsen et Lee, cv. Shang\|Hai\|Qing) and celery ( A.graveolens L. var. dulce DC., cv. Qing\|Qin) were conducted, respectively. The initial soil and four incubated soils with different extractable Cd (0.15, 0.89, 1.38, 1.84 and 2.30 mg Cd/kg soil) were used for the pot experiment. Five treatments were designed (0, 0.0625, 0.125, 0.250 and 0.500 mg Cd/L) in nutrient solution in the sandy culture experiment. Each treatment in pot and sandy culture experiments was trireplicated. The objectives of the study were to examine Cd accumulation in edible parts of selected vegetable crops, its correlation with Cd concentrations in vegetable garden soil or in nutrient solution, and evaluate the criteria of Cd pollution in vegetable garden soil and in nutrient solution based on the hygienic limit of Cd in vegetables. Cadmium concentrations in edible parts of the three selected vegetable crops were as follows: 0.01—0.15 mg/kg fresh weight for Chinese cabbage, 0.02—0.17 mg/kg fresh weight for winter greens, and 0.02—0.24 mg/kg fresh weight for celery in the pot experiment, and 0.1—0.4 mg/kg fresh weight for Chinese cabbage, 0.1—1.4 mg/kg fresh weight for winter greens, and 0.05—0.5 mg/kg fresh weight for celery in the pot experiment(except no\|Cd treatment). The order of the three test vegetable crops for cadmium accumulation in the edible parts was celery>winter greens>Chinese cabbage in both the pot experiment and the sandy culture experiment. Cadmium accumulation in edible parts or roots of the vegetable crops increased with increasing of cadmium concentration in the medium(soil or nutrient solution). And cadmium concentrations in edible parts of the test vegetable crops were significantly linearly related to the Cd levels in the growth media (soil and nutrient solution). Based on the regression equations established and the limit of cadmium concentration in vegetable products, the thresholds of Cd concentration in the growth medium evaluated was as follows: 0.5 mg/kg soil of extractable Cd for soil and 0.02 mg /L for nutrient solution. The high capacity for cadmium accumulation in the edible parts of different vegetable crops together with the absence of visual symptoms implies a potential danger for humans.
