【目的】了解唐家河国家级自然保护区中华蜜蜂的遗传多样性,为进一步保护和利用唐家河中华蜜蜂资源提供科学依据。【方法】以基因片段mt DNA t RNAleu^COⅡ的非编码区作为分子标记,对唐家河各个地区共37个中蜂样本进行序列测定及相关分...【目的】了解唐家河国家级自然保护区中华蜜蜂的遗传多样性,为进一步保护和利用唐家河中华蜜蜂资源提供科学依据。【方法】以基因片段mt DNA t RNAleu^COⅡ的非编码区作为分子标记,对唐家河各个地区共37个中蜂样本进行序列测定及相关分析。【结果】唐家河中蜂的核苷酸多样度(Pi)为0.030 34,单倍型多样性(Hd)为0.839±0.041;共发现14个单倍型,主体单倍型TJH2和TJH4分别占总样本数的29.73%和27.03%。此外本研究首次报道了3个新发现的单倍型。【结论】唐家河中蜂具有丰富的遗传多样性,其单倍型的分布与地理、气候等因素有较大关系。展开更多
[Objective] This study was to clone the GnRH （gonadotropin-releasing hormone）, and to investigate its expression in Apis cerana cerana. [Method] The cDNA sequence of GnRHR gene was amplified from Apis cerana cerana ...[Objective] This study was to clone the GnRH （gonadotropin-releasing hormone）, and to investigate its expression in Apis cerana cerana. [Method] The cDNA sequence of GnRHR gene was amplified from Apis cerana cerana by using RT-PCR techniques. It was conducted with bioinformatics analysis and the in situ hybridization histochemistry of its expression products was studied. [Result] The sequence analy- sis showed that the full cDNA sequence was 1 050 bp with the open reading frame of 1 050 bp, and it encoded 349 amino acid residues. The deduced amino sequence included 7 transmembrane regions, and the predicted molecular mass and isoelectric point were 40.6 kD and 9.54, respectively. The cluster analysis showed that the GnRHR from ＇,4. cerana cerana had close relationship to the GnRHR II from other insects. In situ hybridization showed that Bee-GnRHR staining was specifically localized to the brain, intestine, fat body and testis. [Conclusion] The results indicated that the GnRHR provided molecular bond for the reproduction and metabolism for insects, and suggested a functional role for bee-GnRHR signaling in the coupling of reproduction activities and environment conditions.展开更多
Using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE） and conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the...Using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE） and conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the Chinese honeybee, Apis cerana cerana, was assessed. Honeybees were fed with Bt-transgenic maize pollen, non-transgenic near isoline pollen, linear crylAh gene （800 ng mL^-1） and supercoiled plasmid DNA （800 ng mL^-1） under laboratory conditions. The DGGE profile showed that the number of DGGE bands varied from 10.7 to 14.7 per sample, and the Shannon＇s index ranged from 0.85 to 1.00. The similarity calculated by PAST was mostly above 92%, indicating no obvious changes among treatments or within replicates. 14 bacterial strains affiliated with Alphaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were isolated and characterized on media under aerobic and anaerobic conditions. These results demonstrated that transgenic crylAh maize pollen did not induce significant changes of the honeybee gut bacterial community composition under laboratory conditions.展开更多
By screening the worker （Apis cerana cerana） heads cDNA library using a fragment of the mrjp3 gene of Apis cerana as probe, 120 positive clones were obtained. The clone containing A. cerana cerana MRJP3 （AccMRJP3）...By screening the worker （Apis cerana cerana） heads cDNA library using a fragment of the mrjp3 gene of Apis cerana as probe, 120 positive clones were obtained. The clone containing A. cerana cerana MRJP3 （AccMRJP3） cDNA was selected. Based on the sequencing of the inserts of the positive clone, a sequence of AccMRJP3 cDNA which is 1 887 bp long including a poly （A） tail was obtained. The AccMRJP3 cDNA encompassed an open-reading frame （ORF） with 1 779 bp encoding 593 amino acids. The un-translated regions （UTR） of the 5′ end and 3′end are 46 bp and 160 bp in length, respectively. Similar to AmMRJP3 and AdMRJP3, the putative AccMRJP3 also has a repetitive region. The comparison of the repetitive region of AccMRJP3, AmMRJP3 and AdMRJP3 shows some differences between them.展开更多
Insulin receptors(InR)are an integral component of the insulin/insulin-like growth factor signaling pathway,which plays a vital role in insect development,lifespan,reproduction,and olfactory sensitivity.However,whethe...Insulin receptors(InR)are an integral component of the insulin/insulin-like growth factor signaling pathway,which plays a vital role in insect development,lifespan,reproduction,and olfactory sensitivity.However,whether InR participate in the periph-eral olfactory system of insects remains unclear.Recently,we found that 2-heptanone(2-HT)affects AcerlnR expression,the gene for an InR protein,in Apis cerana cerana.We then examined the spatiotemporal expression profile of the gene in A.cerana cerana.The mRNA of AcerlnR was primarily expressed in the antennae,wings,and legs of forager bees,which are probable chemosensory tissues.The results of fluorescence competitive binding assays,combined with site-directed mutagenesis,demonstrated that AcerOBP6 and AcerOBP14 exhibit strong binding affinities to 2-HT.Furthermore,after foragers were fed with double-stranded AcerlnR,the expression levels of AcerOBP6 and AcerOBP14 decreased significantly,as did the electroantennogram responsiveness to 2-HT and some other odorants.In conclusion,our findings provide a foundation for understanding the in-volvement of AcerlnR in the odor perception of A.cerana cerana.Moreover,they offer novel insights into the olfactory recognition mechanism in insects.展开更多
20 localities were randomly selected in Eastern Black Sea Region of Turkey and samples were collected from around the beehives from April to September. Total of 4,640 dead adult worker bees were examined during the st...20 localities were randomly selected in Eastern Black Sea Region of Turkey and samples were collected from around the beehives from April to September. Total of 4,640 dead adult worker bees were examined during the study. Total infection rate in worker bees was 21.23%. Nosema ceranae was identified in all localities with molecular techniques. Temperature and humidity values were measured from around the beehives during field studies. The infection rate ofN. ceranae increased proportionally with increasing temperature and humidity factors. Humidity was more effective than temperature on the infection rate ofN. ceranae. The seasonal activity ofN. ceranae was studied. The highest infection rates were observed in June and July. N. ceranae infection rate was higher in localities that were in low-altitude than in localities that were in high-altitude.展开更多
文摘【目的】了解唐家河国家级自然保护区中华蜜蜂的遗传多样性,为进一步保护和利用唐家河中华蜜蜂资源提供科学依据。【方法】以基因片段mt DNA t RNAleu^COⅡ的非编码区作为分子标记,对唐家河各个地区共37个中蜂样本进行序列测定及相关分析。【结果】唐家河中蜂的核苷酸多样度(Pi)为0.030 34,单倍型多样性(Hd)为0.839±0.041;共发现14个单倍型,主体单倍型TJH2和TJH4分别占总样本数的29.73%和27.03%。此外本研究首次报道了3个新发现的单倍型。【结论】唐家河中蜂具有丰富的遗传多样性,其单倍型的分布与地理、气候等因素有较大关系。
基金Supported by the Science and Technology Planning Project of the Education Department of Shaanxi Province(11JK0618)~~
文摘[Objective] This study was to clone the GnRH （gonadotropin-releasing hormone）, and to investigate its expression in Apis cerana cerana. [Method] The cDNA sequence of GnRHR gene was amplified from Apis cerana cerana by using RT-PCR techniques. It was conducted with bioinformatics analysis and the in situ hybridization histochemistry of its expression products was studied. [Result] The sequence analy- sis showed that the full cDNA sequence was 1 050 bp with the open reading frame of 1 050 bp, and it encoded 349 amino acid residues. The deduced amino sequence included 7 transmembrane regions, and the predicted molecular mass and isoelectric point were 40.6 kD and 9.54, respectively. The cluster analysis showed that the GnRHR from ＇,4. cerana cerana had close relationship to the GnRHR II from other insects. In situ hybridization showed that Bee-GnRHR staining was specifically localized to the brain, intestine, fat body and testis. [Conclusion] The results indicated that the GnRHR provided molecular bond for the reproduction and metabolism for insects, and suggested a functional role for bee-GnRHR signaling in the coupling of reproduction activities and environment conditions.
基金supported by the National Basic Research Program of China (2007CB109203 and 2009CB118902)
文摘Using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE） and conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the Chinese honeybee, Apis cerana cerana, was assessed. Honeybees were fed with Bt-transgenic maize pollen, non-transgenic near isoline pollen, linear crylAh gene （800 ng mL^-1） and supercoiled plasmid DNA （800 ng mL^-1） under laboratory conditions. The DGGE profile showed that the number of DGGE bands varied from 10.7 to 14.7 per sample, and the Shannon＇s index ranged from 0.85 to 1.00. The similarity calculated by PAST was mostly above 92%, indicating no obvious changes among treatments or within replicates. 14 bacterial strains affiliated with Alphaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were isolated and characterized on media under aerobic and anaerobic conditions. These results demonstrated that transgenic crylAh maize pollen did not induce significant changes of the honeybee gut bacterial community composition under laboratory conditions.
基金the National Natural Science Foundation of China(30200206) Zhejiang Provincial Natural Science Foundation of China(302113).
文摘By screening the worker （Apis cerana cerana） heads cDNA library using a fragment of the mrjp3 gene of Apis cerana as probe, 120 positive clones were obtained. The clone containing A. cerana cerana MRJP3 （AccMRJP3） cDNA was selected. Based on the sequencing of the inserts of the positive clone, a sequence of AccMRJP3 cDNA which is 1 887 bp long including a poly （A） tail was obtained. The AccMRJP3 cDNA encompassed an open-reading frame （ORF） with 1 779 bp encoding 593 amino acids. The un-translated regions （UTR） of the 5′ end and 3′end are 46 bp and 160 bp in length, respectively. Similar to AmMRJP3 and AdMRJP3, the putative AccMRJP3 also has a repetitive region. The comparison of the repetitive region of AccMRJP3, AmMRJP3 and AdMRJP3 shows some differences between them.
基金This research was funded by the earmarked fund for China Agriculture Research System(grant number CARS-44-KXJ22)the Natural Science Foundation of Shanxi Province,China(grant numbers:201901D211356 and 20210302123369).
文摘Insulin receptors(InR)are an integral component of the insulin/insulin-like growth factor signaling pathway,which plays a vital role in insect development,lifespan,reproduction,and olfactory sensitivity.However,whether InR participate in the periph-eral olfactory system of insects remains unclear.Recently,we found that 2-heptanone(2-HT)affects AcerlnR expression,the gene for an InR protein,in Apis cerana cerana.We then examined the spatiotemporal expression profile of the gene in A.cerana cerana.The mRNA of AcerlnR was primarily expressed in the antennae,wings,and legs of forager bees,which are probable chemosensory tissues.The results of fluorescence competitive binding assays,combined with site-directed mutagenesis,demonstrated that AcerOBP6 and AcerOBP14 exhibit strong binding affinities to 2-HT.Furthermore,after foragers were fed with double-stranded AcerlnR,the expression levels of AcerOBP6 and AcerOBP14 decreased significantly,as did the electroantennogram responsiveness to 2-HT and some other odorants.In conclusion,our findings provide a foundation for understanding the in-volvement of AcerlnR in the odor perception of A.cerana cerana.Moreover,they offer novel insights into the olfactory recognition mechanism in insects.
文摘20 localities were randomly selected in Eastern Black Sea Region of Turkey and samples were collected from around the beehives from April to September. Total of 4,640 dead adult worker bees were examined during the study. Total infection rate in worker bees was 21.23%. Nosema ceranae was identified in all localities with molecular techniques. Temperature and humidity values were measured from around the beehives during field studies. The infection rate ofN. ceranae increased proportionally with increasing temperature and humidity factors. Humidity was more effective than temperature on the infection rate ofN. ceranae. The seasonal activity ofN. ceranae was studied. The highest infection rates were observed in June and July. N. ceranae infection rate was higher in localities that were in low-altitude than in localities that were in high-altitude.
