Apium graveolens L. accelerating differentiation of neural stem cells in vitro

To evaluate the action of Apium graveolens L. on the growth and differentiation of neural stem cells （NSCs）, cells culture and animal experiment were performed. NSCs were isolated from the striatum of SD rat embryo were cultured in the medium containing aqueous extract from Apium graveolens L（AAG）.and/or the serum derived from mice treated with Apium graveolens L （SAG）. The results show that AAG promoted the survival and growth of NSCs in low concentration. Apium graveolens L. leaves aqueous extract promoted the proliferation of NSCs in relatively high concentration. SAG significantly accelerated the differentiation of NSCs.

基于高效液相色谱法的芹菜11种酚类化合物分析及应用

以10种芹菜(Apium graveolens L.)为试材,采用Venusil XBP Cl8(L)色谱柱(250 mm×4.6 mm,5μm),以0.2%乙酸溶液-甲醇为流动相,梯度洗脱,流速为0.5 mL/min;采用PDA检测器,检测波长为240 nm。建立了基于高效液相色谱(HPLC)同时测定芹菜中11种酚类化合物的方法,并对色谱柱的选择、流动相条件和梯度洗脱条件等进行优化。结果表明,11种酚类物质在0.5~10.0μg/mL范围内线性关系良好,相关系数均≥0.990。在芹菜样品中进行加标水平为0.2、0.4、1.0 mg/kg的回收试验,回收率为71.90%~134.00%,相对标准偏差(RSD)为0.30%~4.00%。检出限(LOD)与定量限(LOQ)分别为0.07~0.29 mg/kg、0.20~0.84 mg/kg。各活性成分在24 h内稳定性良好,相对标准偏差均小于3.00%。该方法成功应用于10种芹菜中11种酚类化合物含量的测定,结合聚类分析发现不同品种的芹菜酚类组分存在相似性,但其含量存在品种间差异。该方法灵敏度高、精密度好,可为芹菜中11种酚类化合物含量的分析提供技术支持。