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Enhancement of fluorescence of terbium and transferrin-bound diterbium by HEPES
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作者 杜萍 袁兰 +2 位作者 吴后男 徐克宁 杨晓改 《Journal of Chinese Pharmaceutical Sciences》 CAS 2009年第3期218-224,共7页
Terbium (Tb) has been extensively used as a fluorescence probe for the identification of calcium-binding sites in proteins and for fluorometric analysis of organic ligands. In the current study, we reported that HEP... Terbium (Tb) has been extensively used as a fluorescence probe for the identification of calcium-binding sites in proteins and for fluorometric analysis of organic ligands. In the current study, we reported that HEPES, a commonly used pH buffer reagent, significantly enhanced the characteristic emission of Tb at 585 nm. The maximum emission of Tb at 490 nm and 549 um were also enhanced by HEPES to a less extent. Thus, cautions should be taken when quantitative analysis is performed based on the fluorescence emission of Tb at 549 nm, since the emission may vary due to the buffer reagents. Additionally, the fluorescence intensity at 585 um was proportional to the concentration of both HEPES and terbium ions, which might be utilized to develop new fluorometric analytical methods. 展开更多
关键词 Tb^3+ HEPES FLUORESCENCE Tb2Tf apotf
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