目的探讨老年脓毒症患者急性生理学与慢性健康状况评分系统(APACHE)Ⅱ与降钙素原(PCT)及C-反应蛋白(CRP)的相关性。方法选取108例老年脓毒症患者,按APACHEⅡ评分结果分为低危组35例、中危组43例、高危组30例;按治疗结果分为两个亚组:好...目的探讨老年脓毒症患者急性生理学与慢性健康状况评分系统(APACHE)Ⅱ与降钙素原(PCT)及C-反应蛋白(CRP)的相关性。方法选取108例老年脓毒症患者,按APACHEⅡ评分结果分为低危组35例、中危组43例、高危组30例;按治疗结果分为两个亚组:好转组(75例)和恶化组(33例),另选取同期50例老年非脓毒症患者为对照,比较各组间APACHEⅡ评分、PCT及CRP水平,并分析老年脓毒症患者APACHEⅡ评分与PCT及CRP的相关性。结果老年脓毒症组APACHEⅡ评分、PCT及CRP水平均显著高于老年非脓毒症组(P<0.05);中、高危组PCT、CRP水平均显著高于低危组(P<0.05),高危组PCT、CRP水平均显著高于中危组(P<0.05);好转组3、7 d APACHEⅡ评分、PCT及第7天CRP水平均显著低于第1天(P<0.05),恶化组的第3、7天APACHEⅡ评分、PCT及CRP水平均显著高于第1天(P<0.05),恶化组3、7 d APACHEⅡ评分、PCT及CRP水平均显著高于好转组第3、7天(P<0.05);老年脓毒症患者PCT与APACHEⅡ评分呈正相关(r=0.858,P<0.05),CRP与APACHEⅡ评分无显著相关性(P>0.05)。结论老年脓毒症患者PCT水平与APACHEⅡ评分相关,PCT水平可反映老年脓毒症患者病情程度。展开更多
目的探讨Ⅱ型胶原交联C-末端肽(CTX-Ⅱ)、软骨寡聚基质蛋白(COMP)在不同严重程度老年膝关节骨性关节炎患者血清中的表达情况及变化规律。方法 60例老年膝关节骨性关节炎患者按照Kellgren and Lawrence(K-L)分级综合评估患者双侧膝关节...目的探讨Ⅱ型胶原交联C-末端肽(CTX-Ⅱ)、软骨寡聚基质蛋白(COMP)在不同严重程度老年膝关节骨性关节炎患者血清中的表达情况及变化规律。方法 60例老年膝关节骨性关节炎患者按照Kellgren and Lawrence(K-L)分级综合评估患者双侧膝关节严重程度并进行分组。A组:左膝K-L分级+右膝K-L分级≤2;B组:左膝K-L分级+右膝K-L分级>2≤4;C组:左膝K-L分级+右膝K-L分级>4≤6;D组:左膝K-L分级+右膝K-L分级>6≤8。收集患者血清,利用ELISA法检测各组患者血清CTX-Ⅱ、COMP表达水平并进行统计学分析。结果血清CTX-Ⅱ表达水平:A、B、C组呈上升趋势,A、B组差异有显著性(P=0.024),B、C组差异无显著性(P=0.792),C、D组差异有显著性(P=0.018),呈现下降趋势;血清COMP表达水平:A、B、C组呈上升趋势,A、B组差异有显著性(P=0.041),B、C组差异无显著性(P=0.565),C、D组差异有显著性(P=0.003),呈现下降趋势。结论血清CTX-Ⅱ、COMP表达水平随着膝关节骨性关节炎加重逐渐升高,软骨退变快速进展期达到最高,膝关节关节炎晚期下降,可作为潜在的评估老年膝关节骨性关节炎严重程度的生物学指标。展开更多
Apolipoprotein A-Ⅱ(APOA-Ⅱ) is the second most abundant apolipoprotein of high-density lipoprotein(HDL)synthesized mainly by the liver and to a much lesser extent by the intestine. Transgenic mice overexpressing huma...Apolipoprotein A-Ⅱ(APOA-Ⅱ) is the second most abundant apolipoprotein of high-density lipoprotein(HDL)synthesized mainly by the liver and to a much lesser extent by the intestine. Transgenic mice overexpressing human APOA-Ⅱ present abnormal lipoprotein composition and are prone to atherosclerosis, though in humans the role for APOA-Ⅱ in coronary heart disease remains controversial. Here, we investigated the effects of overexpressed APOA-Ⅱ on HDL structure and function, adipose tissue metabolic activity, glucose tolerance and insulin sensitivity. C57BL/6 mice were infected with an adenovirus expressing human APOA-Ⅱ or a control adenovirus Ad GFP, and five days post-infection blood and tissue samples were isolated. APOA-Ⅱ expression resulted in distinct changes in HDL apoproteome that correlated with increased antioxidant and anti-inflammatory activities. No effects on cholesterol efflux from RAW 264.7 macrophages were observed. Molecular analyses in white adipose tissue(WAT) indicated a stimulation of oxidative phosphorylation coupled with respiration for ATP production in mice overexpressing APOA-Ⅱ. Finally, overexpressed APOA-Ⅱ improved glucose tolerance of mice but had no effect on the response to exogenously administered insulin. In summary, expression of APOA-Ⅱ in C57BL/6 mice results in pleiotropic effects with respect to HDL functionality, adipose tissue metabolism and glucose utilization, many of which are beneficial to health.展开更多
C-反应蛋白（C-reactive protein,CRP）是一种在急性期机体发生组织损伤、创伤、感染、炎症或者坏死时候非常敏感的反应蛋白标记物,而且越来越多的研究表明其能有效对患者的病情和预后判断的进行评估。同时急性生理和慢性健康状况评分...C-反应蛋白（C-reactive protein,CRP）是一种在急性期机体发生组织损伤、创伤、感染、炎症或者坏死时候非常敏感的反应蛋白标记物,而且越来越多的研究表明其能有效对患者的病情和预后判断的进行评估。同时急性生理和慢性健康状况评分Ⅱ系统（acute physiology and chronic health evaluationⅡ,APACHEⅡ）也经常被运用到ICU危重症患者的病情评估和预后当中，但是临床上两者联合检测来预测危重症患者的研究较少。展开更多
文摘目的探讨老年脓毒症患者急性生理学与慢性健康状况评分系统(APACHE)Ⅱ与降钙素原(PCT)及C-反应蛋白(CRP)的相关性。方法选取108例老年脓毒症患者,按APACHEⅡ评分结果分为低危组35例、中危组43例、高危组30例;按治疗结果分为两个亚组:好转组(75例)和恶化组(33例),另选取同期50例老年非脓毒症患者为对照,比较各组间APACHEⅡ评分、PCT及CRP水平,并分析老年脓毒症患者APACHEⅡ评分与PCT及CRP的相关性。结果老年脓毒症组APACHEⅡ评分、PCT及CRP水平均显著高于老年非脓毒症组(P<0.05);中、高危组PCT、CRP水平均显著高于低危组(P<0.05),高危组PCT、CRP水平均显著高于中危组(P<0.05);好转组3、7 d APACHEⅡ评分、PCT及第7天CRP水平均显著低于第1天(P<0.05),恶化组的第3、7天APACHEⅡ评分、PCT及CRP水平均显著高于第1天(P<0.05),恶化组3、7 d APACHEⅡ评分、PCT及CRP水平均显著高于好转组第3、7天(P<0.05);老年脓毒症患者PCT与APACHEⅡ评分呈正相关(r=0.858,P<0.05),CRP与APACHEⅡ评分无显著相关性(P>0.05)。结论老年脓毒症患者PCT水平与APACHEⅡ评分相关,PCT水平可反映老年脓毒症患者病情程度。
文摘目的探讨Ⅱ型胶原交联C-末端肽(CTX-Ⅱ)、软骨寡聚基质蛋白(COMP)在不同严重程度老年膝关节骨性关节炎患者血清中的表达情况及变化规律。方法 60例老年膝关节骨性关节炎患者按照Kellgren and Lawrence(K-L)分级综合评估患者双侧膝关节严重程度并进行分组。A组:左膝K-L分级+右膝K-L分级≤2;B组:左膝K-L分级+右膝K-L分级>2≤4;C组:左膝K-L分级+右膝K-L分级>4≤6;D组:左膝K-L分级+右膝K-L分级>6≤8。收集患者血清,利用ELISA法检测各组患者血清CTX-Ⅱ、COMP表达水平并进行统计学分析。结果血清CTX-Ⅱ表达水平:A、B、C组呈上升趋势,A、B组差异有显著性(P=0.024),B、C组差异无显著性(P=0.792),C、D组差异有显著性(P=0.018),呈现下降趋势;血清COMP表达水平:A、B、C组呈上升趋势,A、B组差异有显著性(P=0.041),B、C组差异无显著性(P=0.565),C、D组差异有显著性(P=0.003),呈现下降趋势。结论血清CTX-Ⅱ、COMP表达水平随着膝关节骨性关节炎加重逐渐升高,软骨退变快速进展期达到最高,膝关节关节炎晚期下降,可作为潜在的评估老年膝关节骨性关节炎严重程度的生物学指标。
基金supported financially by the program"Support of Young Investigators"MIS No.5005458 that was co-financed by the Operational Program"Human Resources Development,Education and Lifelong Learning"and by the European Union(European Social Fund)and Greek national funds。
文摘Apolipoprotein A-Ⅱ(APOA-Ⅱ) is the second most abundant apolipoprotein of high-density lipoprotein(HDL)synthesized mainly by the liver and to a much lesser extent by the intestine. Transgenic mice overexpressing human APOA-Ⅱ present abnormal lipoprotein composition and are prone to atherosclerosis, though in humans the role for APOA-Ⅱ in coronary heart disease remains controversial. Here, we investigated the effects of overexpressed APOA-Ⅱ on HDL structure and function, adipose tissue metabolic activity, glucose tolerance and insulin sensitivity. C57BL/6 mice were infected with an adenovirus expressing human APOA-Ⅱ or a control adenovirus Ad GFP, and five days post-infection blood and tissue samples were isolated. APOA-Ⅱ expression resulted in distinct changes in HDL apoproteome that correlated with increased antioxidant and anti-inflammatory activities. No effects on cholesterol efflux from RAW 264.7 macrophages were observed. Molecular analyses in white adipose tissue(WAT) indicated a stimulation of oxidative phosphorylation coupled with respiration for ATP production in mice overexpressing APOA-Ⅱ. Finally, overexpressed APOA-Ⅱ improved glucose tolerance of mice but had no effect on the response to exogenously administered insulin. In summary, expression of APOA-Ⅱ in C57BL/6 mice results in pleiotropic effects with respect to HDL functionality, adipose tissue metabolism and glucose utilization, many of which are beneficial to health.
文摘C-反应蛋白（C-reactive protein,CRP）是一种在急性期机体发生组织损伤、创伤、感染、炎症或者坏死时候非常敏感的反应蛋白标记物,而且越来越多的研究表明其能有效对患者的病情和预后判断的进行评估。同时急性生理和慢性健康状况评分Ⅱ系统（acute physiology and chronic health evaluationⅡ,APACHEⅡ）也经常被运用到ICU危重症患者的病情评估和预后当中，但是临床上两者联合检测来预测危重症患者的研究较少。
