AIM:To investigate the relationship between Apolipoprotein C3(APOC3)(-455T>C) polymorphism and nonalcoholic fatty liver disease(NAFLD) in the Southern Chinese han population.METHODS:In this prospective case-control...AIM:To investigate the relationship between Apolipoprotein C3(APOC3)(-455T>C) polymorphism and nonalcoholic fatty liver disease(NAFLD) in the Southern Chinese han population.METHODS:In this prospective case-control study,we recruited 300 NAFLD patients and 300 healthy controls to a cohort representing Southern Chinese han population at The First Affiliated Hospital,Sun Yat-sen University,from January to December 2012. Polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing were used to genotype the APOC3(-455T>C) variants.RESULTS:After adjusting for age,gender,and bodymass index,TC and CC genotypes were found to increase the susceptibility to NAFLD compared to the TT genotype,with adjusted odds ratios(ORs) of 1.77(95%CI:1.16-2.72) and 2.80(95%CI:1.64-4.79),respectively. Further stratification analysis indicated that carriers of the CC genotype was more susceptible to insulin resistance(IR) than those of the TT genotype,with an OR of 3.24(95%CI:1.52-6.92). The CC genotype also was associated with a significantly higher risk of hypertension,hypertriglyceridemia,and low levels of high-density lipoprotein cholesterol(hDL)(P < 0.05). No association was found between the APOC3(-455T>C) polymorphism and obesity,impaired glucose tolerance,hyperuricemia,hypercholesterolemia,or high levels of low-density lipoprotein cholesterol(LDL)(P > 0.05).CONCLUSION:APOC3(-455T>C) genetic variation is involved in the susceptibility to developing NAFLD,IR,hypertension,hypertriglyceridemia,and low hDL in the Southern Chinese han population.展开更多
AIM:To investigate the association between two polymorphisms of apolipoprotein C3(APOC3)and risk of nonalcoholic fatty liver disease(NAFLD)in a Chinese Han population.METHODS:Genotypes for rs2854116 and rs2854117in AP...AIM:To investigate the association between two polymorphisms of apolipoprotein C3(APOC3)and risk of nonalcoholic fatty liver disease(NAFLD)in a Chinese Han population.METHODS:Genotypes for rs2854116 and rs2854117in APOC3 and the known rs738409 in patatin-like phospholipase domain-containing protein 3(PNPLA3)in 390patients with NAFLD and 409 control subjects were determined by sequencing and polymerase chain reaction analysis.Serum lipid profiles were determined using biochemical methods,and an index of insulin resistance(IR,HOMA-IR),serum APOC3 concentrations and total antioxidant status(TAS)were also assessed.RESULTS:No significant differences in genotype and allele frequencies of rs2854116 and rs2854117 were found between the NAFLD population and the controls(P>0.05).The OR for the association between-455Cand-482T allele carriers and the risk of NAFLD were1.06(95%CI:0.72-1.57,P>0.05)and 1.00(95%CI:0.68-1.48,P>0.05),respectively.The variant carriers did not have a significantly increased risk of NAFLD or elevated clinical and biochemical parameters such as APOC3 concentrations,IR(1.42±0.43 vs 1.48±0.52,P>0.05),liver enzymes and TAS(13.94±2.01vs 14.38±1.92,P>0.05)compared with the controls.Moreover,the results were similar when testing was carried out independent of the genetic variation in PNPLA3.CONCLUSION:The two polymorphisms of the APOC3gene are not associated with a risk of NAFLD,or with lipid profiles,IR and oxidative stress in the Chinese Han population.展开更多
基金Supported by Natural Scientific Foundation of Guangdong Province,No.S2012040007685Doctoral Program Foundation of Institutions of Higher Education of China,No.20120171120090National Natural Science Foundation of China,No.81301769 and No.81170392
文摘AIM:To investigate the relationship between Apolipoprotein C3(APOC3)(-455T>C) polymorphism and nonalcoholic fatty liver disease(NAFLD) in the Southern Chinese han population.METHODS:In this prospective case-control study,we recruited 300 NAFLD patients and 300 healthy controls to a cohort representing Southern Chinese han population at The First Affiliated Hospital,Sun Yat-sen University,from January to December 2012. Polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing were used to genotype the APOC3(-455T>C) variants.RESULTS:After adjusting for age,gender,and bodymass index,TC and CC genotypes were found to increase the susceptibility to NAFLD compared to the TT genotype,with adjusted odds ratios(ORs) of 1.77(95%CI:1.16-2.72) and 2.80(95%CI:1.64-4.79),respectively. Further stratification analysis indicated that carriers of the CC genotype was more susceptible to insulin resistance(IR) than those of the TT genotype,with an OR of 3.24(95%CI:1.52-6.92). The CC genotype also was associated with a significantly higher risk of hypertension,hypertriglyceridemia,and low levels of high-density lipoprotein cholesterol(hDL)(P < 0.05). No association was found between the APOC3(-455T>C) polymorphism and obesity,impaired glucose tolerance,hyperuricemia,hypercholesterolemia,or high levels of low-density lipoprotein cholesterol(LDL)(P > 0.05).CONCLUSION:APOC3(-455T>C) genetic variation is involved in the susceptibility to developing NAFLD,IR,hypertension,hypertriglyceridemia,and low hDL in the Southern Chinese han population.
基金Supported by National Natural Science Foundation of China,No.81170337/H0304
文摘AIM:To investigate the association between two polymorphisms of apolipoprotein C3(APOC3)and risk of nonalcoholic fatty liver disease(NAFLD)in a Chinese Han population.METHODS:Genotypes for rs2854116 and rs2854117in APOC3 and the known rs738409 in patatin-like phospholipase domain-containing protein 3(PNPLA3)in 390patients with NAFLD and 409 control subjects were determined by sequencing and polymerase chain reaction analysis.Serum lipid profiles were determined using biochemical methods,and an index of insulin resistance(IR,HOMA-IR),serum APOC3 concentrations and total antioxidant status(TAS)were also assessed.RESULTS:No significant differences in genotype and allele frequencies of rs2854116 and rs2854117 were found between the NAFLD population and the controls(P>0.05).The OR for the association between-455Cand-482T allele carriers and the risk of NAFLD were1.06(95%CI:0.72-1.57,P>0.05)and 1.00(95%CI:0.68-1.48,P>0.05),respectively.The variant carriers did not have a significantly increased risk of NAFLD or elevated clinical and biochemical parameters such as APOC3 concentrations,IR(1.42±0.43 vs 1.48±0.52,P>0.05),liver enzymes and TAS(13.94±2.01vs 14.38±1.92,P>0.05)compared with the controls.Moreover,the results were similar when testing was carried out independent of the genetic variation in PNPLA3.CONCLUSION:The two polymorphisms of the APOC3gene are not associated with a risk of NAFLD,or with lipid profiles,IR and oxidative stress in the Chinese Han population.
