目的:探讨X-连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP)和caspase-3在宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)及宫颈鳞癌(squamous carcinoma of the cervix,SCC)中的表达水平及其与高危型人乳...目的:探讨X-连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP)和caspase-3在宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)及宫颈鳞癌(squamous carcinoma of the cervix,SCC)中的表达水平及其与高危型人乳头瘤病毒(human papilloma virus,HPV)感染的关系。方法:采用免疫组织化学Envision二步法检测XIAP和caspase-3在SCC(n=25)、CIN(n=80)及正常宫颈(normal cervix,NC,n=10)石蜡包埋组织中的表达,并进行HPV分型检测。结果:高危型HPV总感染率为62.6%(72/115)。SCC、CINⅢ、CINⅡ、CINⅠ、NC组的高危型HPV感染率分别为88.0%(22/25)、84.0%(21/25)、68.0%(17/25)、33.3%(10/30)、20.0%(2/10),高危型HPV感染率随着宫颈病变的加重呈上升趋势,差异有统计学意义(χ2=30.820,P=0.000)。NC、CINⅠ、CINⅡ与SCC组的XIAP阳性表达率两两比较差异均有统计学意义(P均<0.01),XIAP阳性率及表达水平与宫颈病变的严重程度呈正相关(r=0.410,P=0.000)。NC、CINⅠ、CINⅡ、CINⅢ与SCC组的caspase-3阳性表达率两两比较差异均有统计学意义(P均<0.05),其阳性率及表达水平与宫颈病变的严重程度呈负相关(r=-0.320,P=0.000)。在CIN及SCC中,XIAP与caspase-3、XIAP与高危型HPV感染分别呈负相关(r=-0.402,P=0.000)和正相关(r=0.458,P=0.000)。结论:高危型HPV感染、XIAP高表达和caspase-3表达下调在宫颈癌前病变向SCC演变的过程中可能起促进作用。展开更多
This survey points to the mechanisms of bioresponse caused by magnetic fields (MFs), paying attention to their action not only on ions, molecules and macromolecules, but also on cells, tissues and organisms. The signi...This survey points to the mechanisms of bioresponse caused by magnetic fields (MFs), paying attention to their action not only on ions, molecules and macromolecules, but also on cells, tissues and organisms. The significance of findings concerning the MF-dependence of cell proliferation, necrosis or apoptosis was judged by comparing the results obtained in a solenoid, where an MF can be added to the geomagnetic field (GMF), with those obtained in a magnetically shielded room, where the MFs can be attenuated or null. This comparative criterion was particularly appropriate when the differences detectable between the data provided by experimental samples and the data provided by control samples were rather small, as observed in estimating the MF-influence on total DNA replication, RNA transcription and polypeptide translation. The MF-induced inhibition of apoptosis was considered as a risk potentially leading to accumulation of cancer cells. The analysis also surveyed the MF-dependence of the interactions between host animal cells and infecting bacteria. In relation to studies on the origin and adaptation of life on the Earth, theoretical insights paving the way to elucidating the MF-interactions with biostructures and biosystems of different orders of organization evaluated the possible involvement of the so-called “biological windows”. Analogously to what is known for ionizing radiations, the efficiency of the applied MFs appeared to depend on the complexity of their biological targets.展开更多
Objective: To study the effects of anti-HPV bioprotein dressing combined with interferon α-2b therapy on the malignant molecule expression in patients with cervical intraepithelial neoplasia (CIN) Ⅲ complicated by h...Objective: To study the effects of anti-HPV bioprotein dressing combined with interferon α-2b therapy on the malignant molecule expression in patients with cervical intraepithelial neoplasia (CIN) Ⅲ complicated by high-risk HPV positive. Methods: Patients who were diagnosed with CINⅢ and high-risk HPV positive and underwent conization in the 3201 Hospital Affiliated to Xi'an Jiaotong University between June 2014 and February 2017 were selected and randomly divided into the observation group who received preoperative anti-HPV bioprotein dressing combined with interferon α-2b therapy and the control group who received no special treatment. CIN lesion was collected to determine the expression of pro-proliferation molecules, pro-apoptosis molecules and epithelial-mesenchymal transition molecules. Results: Rsf1, Piwil2, TOPK, p38MAPK, ERK, Snail, Twist, N-cadherin and Vimentin mRNA expression in cervical intraepithelial neoplasia lesions of observation group were greatly lower than those of control group whereas LRIG3, SARI, IEX-1, FHIT and E-cadherin mRNA expression were greatly higher than those of control group. Conclusion: Anti-HPV bioprotein dressing combined with interferon α-2b therapy can inhibit the proliferation and invasive growth of tumor cells in patients with CINⅢ complicated by high-risk HPV positive.展开更多
文摘目的:探讨X-连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein,XIAP)和caspase-3在宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)及宫颈鳞癌(squamous carcinoma of the cervix,SCC)中的表达水平及其与高危型人乳头瘤病毒(human papilloma virus,HPV)感染的关系。方法:采用免疫组织化学Envision二步法检测XIAP和caspase-3在SCC(n=25)、CIN(n=80)及正常宫颈(normal cervix,NC,n=10)石蜡包埋组织中的表达,并进行HPV分型检测。结果:高危型HPV总感染率为62.6%(72/115)。SCC、CINⅢ、CINⅡ、CINⅠ、NC组的高危型HPV感染率分别为88.0%(22/25)、84.0%(21/25)、68.0%(17/25)、33.3%(10/30)、20.0%(2/10),高危型HPV感染率随着宫颈病变的加重呈上升趋势,差异有统计学意义(χ2=30.820,P=0.000)。NC、CINⅠ、CINⅡ与SCC组的XIAP阳性表达率两两比较差异均有统计学意义(P均<0.01),XIAP阳性率及表达水平与宫颈病变的严重程度呈正相关(r=0.410,P=0.000)。NC、CINⅠ、CINⅡ、CINⅢ与SCC组的caspase-3阳性表达率两两比较差异均有统计学意义(P均<0.05),其阳性率及表达水平与宫颈病变的严重程度呈负相关(r=-0.320,P=0.000)。在CIN及SCC中,XIAP与caspase-3、XIAP与高危型HPV感染分别呈负相关(r=-0.402,P=0.000)和正相关(r=0.458,P=0.000)。结论:高危型HPV感染、XIAP高表达和caspase-3表达下调在宫颈癌前病变向SCC演变的过程中可能起促进作用。
文摘This survey points to the mechanisms of bioresponse caused by magnetic fields (MFs), paying attention to their action not only on ions, molecules and macromolecules, but also on cells, tissues and organisms. The significance of findings concerning the MF-dependence of cell proliferation, necrosis or apoptosis was judged by comparing the results obtained in a solenoid, where an MF can be added to the geomagnetic field (GMF), with those obtained in a magnetically shielded room, where the MFs can be attenuated or null. This comparative criterion was particularly appropriate when the differences detectable between the data provided by experimental samples and the data provided by control samples were rather small, as observed in estimating the MF-influence on total DNA replication, RNA transcription and polypeptide translation. The MF-induced inhibition of apoptosis was considered as a risk potentially leading to accumulation of cancer cells. The analysis also surveyed the MF-dependence of the interactions between host animal cells and infecting bacteria. In relation to studies on the origin and adaptation of life on the Earth, theoretical insights paving the way to elucidating the MF-interactions with biostructures and biosystems of different orders of organization evaluated the possible involvement of the so-called “biological windows”. Analogously to what is known for ionizing radiations, the efficiency of the applied MFs appeared to depend on the complexity of their biological targets.
基金Natural Science Foundation Project of Shaanxi Province No:2013CM.
文摘Objective: To study the effects of anti-HPV bioprotein dressing combined with interferon α-2b therapy on the malignant molecule expression in patients with cervical intraepithelial neoplasia (CIN) Ⅲ complicated by high-risk HPV positive. Methods: Patients who were diagnosed with CINⅢ and high-risk HPV positive and underwent conization in the 3201 Hospital Affiliated to Xi'an Jiaotong University between June 2014 and February 2017 were selected and randomly divided into the observation group who received preoperative anti-HPV bioprotein dressing combined with interferon α-2b therapy and the control group who received no special treatment. CIN lesion was collected to determine the expression of pro-proliferation molecules, pro-apoptosis molecules and epithelial-mesenchymal transition molecules. Results: Rsf1, Piwil2, TOPK, p38MAPK, ERK, Snail, Twist, N-cadherin and Vimentin mRNA expression in cervical intraepithelial neoplasia lesions of observation group were greatly lower than those of control group whereas LRIG3, SARI, IEX-1, FHIT and E-cadherin mRNA expression were greatly higher than those of control group. Conclusion: Anti-HPV bioprotein dressing combined with interferon α-2b therapy can inhibit the proliferation and invasive growth of tumor cells in patients with CINⅢ complicated by high-risk HPV positive.
