Multinationals＇ Environmental Colonialism： A Case Study of Apple Inc. and China＇s Countermeasures

Multinational firms such as Apple Inc. have accomplished environmental colonialism in China through the transfer of ecological costs to the local environment. This paper discusses ambiguities in China＇s environmental legislation and supervision, Apple＇s business model, and ＂green washing. ＂ Further, we have identified countermeasures such as the encouraging Environmental Non-Governmental Organizations （ENGOs）, streamlining litigation procedures, increasing fines, and the development of nonprofit media organizations.

Spatial Distribution of Meteorological Factors During the Key Growth Periods of Apple Cultivation in Longdong Region of Gansu Province

With the ongoing development of the apple industry in the Longdong region of Gansu,this sector has emerged as a key driver for the government s initiatives aimed at increasing stable income for local residents and promoting rural revitalization.The Longdong region boasts a favorable geographical location and a suitable climatic environment,making it an ideal area for apple cultivation.This paper analyzes meteorological data from the national meteorological observatory in Longdong over the past forty years,focusing on average temperature,precipitation,sunshine duration,and relative humidity during three critical growth periods of apples.The research reveals significant differences in the distribution of meteorological conditions across these key growth stages.Notably,the average temperature is higher in the central and northern parts of the region,while lower temperatures are observed in the southwestern areas.The average daily maximum temperature tends to be higher in the northwest and lower in the central and southwestern regions.Conversely,the average daily minimum temperature demonstrates a distinct pattern,being higher in the south and lower in the north.Additionally,precipitation is more abundant in the southeast and less so in the northwest.Sunshine hours are greater in the northern and central regions,while the southwestern and northeastern areas receive fewer hours of sunlight.Finally,relative humidity is higher in the south and lower in the north.

Effect of Different Fertilizing Methods on Zinc Nutrition in Zn-Deficiency Apple Trees

Red Fuji apple trees of Zn-deficiency were used as material in the experiment. The effect of different Zn application methods on the regularity of Zn transportation and nutrition was studied. It was found that the storage of Zn in the branches was greatly increased by foliar application of the solution of 15% ZnSO4·7H2O before abscission. It had advantage to keep suitable Zn content in leaves, branches and roots, as well as between Zn and P and K, which increased Zn transportation and reusability. Zn content in roots increased when Zn was used in soil in autumn. However, the absorbed Zn did not transport to the top of the plant. It was used by the plant next year. Foliar application of ZnSO4·7H2O at early stage of the plant growth enhanced Zn content of the branch. However, the effect was kept for a short time. It had little effect on Zn transportation when Zn was sprayed three weeks after blossoming.

Effects of Different Times of Bagging on Fruit Quality and Disease and Pest Incidence of Fuji Apple

The effects of bagging at different times in Mengyin area on the fruit quality and disease and pest incidence of Huimin short-cut Fuji apple were investigated,with unbagged fruit as the control. The results showed that among the apples bagged on May 5,May 15,May 25,June 2 and June 12,the single fruit weight and fruit shape index of the apples bagged on June 2 were highest,308. 1 g and 0. 868,respectively; the peeled harness of the apples bagged during May 5-25 was higher than that of the control; the peeled harness of the apples bagged on June 2 and June 12 was lower than that of the control; the soluble solids content of bagged fruit was lower than that of unbagged ones,and no significant differences were found among different treatments; the coloring index of the apples bagged on June 2 was up to 100%; the smooth finish index of the apples bagged on May 5 reached 96. 67%,the highest; the red chromatic value( a*) of bagged fruit was higher than that of unbagged ones,and there were no significant differences among different bagging treatments. The earlier the bagging was,the higher the incidence of black spot and bitter pit was. The incidence of black spot and bitter pit in bagged fruit was higher than that in unbagged ones. In conclusion,in Mengyin area of Shandong Province,bagging is better to be carried out on June 2,45 d after the full bloom.

Study on the clustering of a typical industry:a case of apple industry in Shaanxi Province, China

The apple industry in Shaanxi Province is a resource-based industry; compared to the best productiveregions at home and abroad, it is advantageous in high quality as well as geographical and natural resources. Currently,big changes have occurred in the apple market supply-demand relations; long-term and comprehensive shortage haspassed; the buyer’s market has come into being; competitions in the domestic market are fierce. At the same time, theforeign apple will impact on the domestic market, increasing competitive pressure. Foreign anti-dumping measures,green barriers and technologies restrict the exportation of the domestic apple. Based on successful experience of otherindustrial clusters, this paper analyzes the existing problems in the development of Shaanxi apple industrial cluster,combining empirical analysis, qualitative analysis and quantitative analysis, proposes specific policy recommendationsand get the conclusions. First, the characteristics of the agricultural industrial cluster are analyzed; Shaanxi appleindustry is characterized by cluster. Second, the rapid development of Shaanxi apple industrial cluster exposes someproblems. Third, we need the government to control the disorderly competition of Shaanxi apple industry.

Overexpression of the transcription factor MdWRKY115 improves drought and osmotic stress tolerance by directly binding to the MdRD22 promoter in apple

Abiotic stress reduces plant yield and quality.WRKY transcription factors play key roles in abiotic stress responses in plants,but the molecular mechanisms by which WRKY transcription factors mediate responses to drought and osmotic stresses in apple(Malus×domestica Borkh.)remain unclear.Here,we functionally characterized the apple GroupⅢWRKY gene MdWRKY115.qRT-PCR analysis showed that MdWRKY115 expression was up-regulated by drought and osmotic stresses.GUS activity analysis revealed that the promoter activity of MdWRKY115 was enhanced under osmotic stress.Subcellular localization and transactivation assays indicated that MdWRKY115 was localized to the nucleus and had a transcriptional activity domain at the N-terminal region.Transgenic analysis revealed that the overexpression of MdWRKY115 in Arabidopsis plants and in apple callus markedly enhanced their tolerance to drought and osmotic stresses.DNA affinity purification sequencing showed that MdWRKY115 binds to the promoter of the stress-related gene MdRD22.This binding was further verified by an electrophoretic mobility shift assay.Collectively,these findings suggest that MdWRKY115 is an important regulator of osmotic and drought stress tolerance in apple.

A Study of the Major Pathogens Causing Fruit Rots of Apple in Shelf Life in Hangzhou, Zhejiang Province, China

Major pathogens causing fruit rots of apple in shelf life in Hangzhou, a city in east China, were identified by rDNA-ITS analysis. Their pathogenicities and stress tolerances were compared as well. Combining with disease symptoms, colonial phenotypes and mycelial microscopic morphology, the fungi were determined as Penicillium expansum, Botrytis cinerea, Botryosphaeria dothidea, Diaporthe phaseolorum, Alternaria alternata and Fusarium acuminatum, respectively. Among them, B. cinerea and B. dothidea showed a higher pathogenicity;B. cinerea and D. phaseolorum were hardly affected by the temperature at a range of 15°C and 25°C;B. cinerea has the highest resistant to Thiabendazole and D. phaseolorum displayed the strongest resistance to Imazalil;and P. expansum was most sensitive to ultraviolet light radiation. The results provide some useful information that helps to combine conventional and alternative control strategies to minimize apple postharvest losses in shelf life.

Inclusion Levels of Fermented Apple Bagasse on in Vitro Rumen Fermentation of Alfalfa Hay

The aim of this study was to assess the effect of inclusion of fermented apple bagasse （FAB） obtained through solid state fermentation on pH, ammonia nitrogen （N-NH3）, volatile fatty acids （VFA） content, in vitro dry matter digestibility （IVDMD）, lactic acid and microbial counting of alfalfa hay under in vitro rumen environment; four levels of FAB were evaluated （0, 0.25, 0.50 and 0. 75 g/dry matter of FAB） replacing 1.5 g dry matter （DM） of alfalfa hay and incubated at different fermentation times （0, 4, 8, 12 and 24 h） using a complete random design with repeated measures on time. Counts of live yeast colonies （6.08, 6.33, 6.24 and 6.51 CFU/mL expressed as log 10） was higher when FAB was included in the different levels up to the 12 h of fermentation （P 〈 0.0001）; lactic acid content also increased as FAB was included in the different levels （10.61, 13.86, 16.84 and 14.57μg/mL） up to the 12 h of incubation （P 〈 0.001）. Nevertheless, the other variables measured as pH, N-NH3, VFA, IVDMD, total bacteria and fungi counts, were not affected by the treatments. It is concluded that substitution of FAB by alfalfa hay in an in vitro rumen ecosystem positively modified live yeast colonies and lactic acid concentration, without effect on the other fermentative and microbial parameters of the in vitro rumen environment, but considering mixes of FAB and alfalfa hay as a quality ingredient for the feeding of ruminants.

Control Effect of Zinc Fertilizer Application Against Apple Little Leaf

In order to provide a theoretical basis for apple cultivation techniques and to provide a reference for preparation of scientific management measures,the occurrence and damage status of apple little leaf in northern Guizhou was investigated,and fertilizer control test against the disease was also conducted.（1）Apple little leaf occurred from April to October in apple producing areas in northern Guizhou every year,with heavy incidence from April to May,light incidence from June to July,and the lightest incidence from August to October.（2） Effectiveness test showed that application of zinc sulfate fertilizer 135 g ＋ cow dung 10 kg received the best control effect,followed by zinc sulfate fertilizer 165 g ＋ cow dung 10 kg,then zinc sulfate fertilizer 105 g ＋ cow dung 10 kg and zinc sulfate fertilizer 150 g ＋ cow dung 10 kg.（3） Based on local environmental conditions,occurrence and damage situation,incidence conditions and fertilizer efficacy test,comprehensive prevention and control technology with rational supplement of zinc fertilizer could be used to control apple little leaf.

Comparative transcriptome analysis reveals key genes and pathways in response to Alternaria alternata apple pathotype infection

Apple leaf spot,caused by the Alternaria alternata apple pathotype(AAAP),is an important fungal disease of apple.To understand the molecular basis of resistance and pathogenesis in apple leaf spot,the transcriptomes of two apple cultivars‘Hanfu'(HF)(resistant)and‘Golden Delicious'(GD)(susceptible)were analyzed at 0,6,18,24 and 48 h after AAAP inoculation by RNA-Seq.At each time point,a large number of significantly differentially expressed genes(DEGs)were screened between AAAP-inoculated and uninoculated apple leaves.Analysis of the common DEGs at four time points revealed significant differences in the resistance of‘HF'and‘GD'apple to AAAP infection.RLP,RNL,and JA signal-related genes were upregulated in both cultivars to restrict AAAP development.However,genes encoding CNLs,TNLs,WRKYs,and AP2s were only activated in‘HF'as part of the resistance response,of which,some play major roles in the regulation of ET and SA signal transduction.Further analysis showed that many DEGs with opposite expression trends in the two hosts may play important regulatory roles in response to AAAP infection.Transient expression of one such gene MdERF110 in‘GD'apple leaves improved AAAP resistance.Collectively,this study highlights the reasons for differential resistance to AAAP infection between‘HF'and‘GD'apples which can theoretically assist the molecular breeding of disease-resistant apple crops.

Genome-wide identification,molecular evolution,and functional characterization of fructokinase gene family in apple reveal its role in improving salinity tolerance

Fructokinase(FRK)is a regulator of fructose signaling in plants and gateway proteins that catalyze the initial step in fructose metabolism through phosphorylation.Our previous study demonstrated that MdFRK2 protein exhibit not only high affinity for fructose,but also high enzymatic activity due to sorbitol.However,genome-wide identification of the MdFRK gene family and their evolutionary dynamics in apple are yet to be reported.A systematic genome-wide analysis in this study identified a total of nine MdFRK gene members,which could phylogenetically be clustered into seven groups.Chromosomal location and synteny analysis of MdFRKs revealed that their expansion in the apple genome is primarily driven by tandem and segmental duplication events.Divergent expression patterns of MdFRKs were observed in four source-sink tissues and at five different apple fruit developmental stages,which suggested their potential crucial roles in the apple fruit development and sugar accumulation.Reverse transcription-quantitative PCR(RT-qPCR)identified candidate NaCl or drought stress responsive MdFRKs,and transgenic apple plants overexpressing MdFRK2 exhibited considerably enhanced salinity tolerance.Our results will be useful for understanding the functions of MdFRKs in the regulation of apple fruit development and salt stress response.

The role of the auxin-response genes MdGH3.1 and MdSAUR36 in bitter pit formation in apple

Apples often exhibit bitter pits in response to metabolic disorders during ripening and storage;however, the mechanisms underlying the bitter pit(BP) development remain unclear. Here, metabolome and transcriptome analyses were performed to investigate BP pulp of 'Fuji'. Two auxin-response genes, MdGH3.1 and MdSAUR36, were screened. Their expression as well as the auxin content in BP pulp were found to be higher than those in healthy pulp(P < 0.01). In the field, excess CO(NH2)2increased the incidence of BP. Moreover, the auxin content and MdGH3.1 expression increased in apples after nitrogen fertilization. On Day 30 before harvest, the two genes were transiently transferred to the fruit, and 20.69% and 23.21% of BP fruits were harvested. After 10 μmol·L-1auxin was infiltrated at low pressure into postharvest fruit, the increase in MdGH3.1 expression occurred earlier than that in MdSAUR36. MdGH3.1 increased the expression of MdSAUR36, but MdSAUR36 did not increase expression of MdGH3.1. Therefore, we suggest that MdGH3.1 acts upstream of MdSAUR36 during BP formation and that these genes induce BP formation by regulating auxin and phenylpropanoid biosynthesis.

Silencing of early auxin responsive genes MdGH3-2/12 reduces the resistance to Fusarium solani in apple

Apple replant disease(ARD)has led to severe yield and quality reduction in the apple industry.Fusarium solani(F.solani)has been identified as one of the main microbial pathogens responsible for ARD.Auxin(indole-3-acetic acid,IAA),an endogenous hormone in plants,is involved in almost all plant growth and development processes and plays a role in plant immunity against pathogens.Gretchen Hagen3(GH3)is one of the early/primary auxin response genes.The aim of this study was to evaluate the function of MdGH3-2 and MdGH3-12 in the defense response of F.solani by treating MdGH3-2/12 RNAi plants with F.solani.The results show that under F.solani infection,RNAi of MdGH3-2/12 inhibited plant biomass accumulation and exacerbated root damage.After inoculation with F.solani,MdGH3-2/12 RNAi inhibited the biosynthesis of acid-amido synthetase.This led to the inhibition of free IAA combining with amino acids,resulting in excessive free IAA accumulation.This excessive free IAA altered plant tissue structure,accelerated fungal hyphal invasion,reduced the activity of antioxidant enzymes(SOD,POD and CAT),increased the reactive oxygen species(ROS)level,and reduced total chlorophyll content and photosynthetic ability,while regulating the expression of PR-related genes including PR1,PR4,PR5 and PR8.It also changed the contents of plant hormones and amino acids,and ultimately reduced the resistance to F.solani.In conclusion,these results demonstrate that MdGH3-2 and MdGH3-12 play an important role in apple tolerance to F.solani and ARD.

Transcriptional regulation of MdPIN7 by MdARF19 during gravityinduced formation of adventitious root GSA in self-rooted apple stock

Self-rooted apple stock is widely used for apple production.However,the shallowness of the adventitious roots in self-rooted apple stock leads to poor performance in the barren orchards of China.This is because of the considerable difference in the development of a gravitropic set-point angle(GSA)between self-rooted apple stock and seedling rootstock.Therefore,it is crucial to study the molecular mechanism of adventitious root GSA in self-rooted apple stock for breeding self-rooted and deep-rooted apple rootstock cultivars.An apple auxin response factor MdARF19 functioned to establish the adventitious root GSA of self-rooted apple stock in response to gravity and auxin signals.MdARF19 bound directly to the MdPIN7 promoter,activating its transcriptional expression and thus regulating the formation of the adventitious root GSA in 12-2 self-rooted apple stock.However,MdARF19 influenced the expression of auxin efflux carriers(MdPIN3 and MdPIN10)and the establishment of adventitious root GSA of self-rooted apple stock in response to gravity signals by direct activation of MdFLP.Our findings provide new information on the transcriptional regulation of MdPIN7 by auxin response factor MdARF19 in the regulation of the adventitious root GSA of self-rooted apple stock in response to gravity and auxin signals.

Overexpression of auxin/indole-3-acetic acid gene MdIAA24 enhances Glomerella leaf spot resistance in apple(Malus domestica)

Auxin is throughout the entire life process of plants and is involved in the crosstalk with other hormones,yet its role in apple disease resistance remains unclear.In this study,we investigated the function of auxin/indole-3-acetic acid(IAA)gene Md IAA24 overexpression in enhancing apple resistance to Glomerella leaf spot(GLS)caused by Colletotrichum fructicola(Cf).Analysis revealed that,upon Cf infection,35S::Md IAA24 plants exhibited enhanced superoxide dismutase(SOD)and peroxidase(POD)activity,as well as a greater amount of glutathione(reduced form)and ascorbic acid accumulation,resulting in less H_(2)O_(2)and superoxide anion(O_(2)^(-))in apple leaves.Furthermore,35S::Md IAA24 plants produced more protocatechuic acid,proanthocyanidins B1,proanthocyanidins B2 and chlorogenic acid when infected with Cf.Following Cf infection,35S::Md IAA24 plants presented lower levels of IAA and jasmonic acid(JA),but higher levels of salicylic acid(SA),along with the expression of related genes.The overexpression of Md IAA24 was observed to enhance the activity of chitinase andβ-1,3-glucanase in Cfinfected leaves.The results indicated the ability of Md IAA24 to regulate the crosstalk between IAA,JA and SA,and to improve reactive oxygen species(ROS)scavenging and defense-related enzymes activity.This jointly contributed to GLS resistance in apple.

Isolation of Trichoderma virens 6PS-2 and its effects on Fusarium proliferatum f. sp. Malus domestica MR5 related to apple replant disease in China

Apple replant disease(ARD) is primarily caused by biotic factors that seriously inhibits the development of apple industry. Therefore, the use of biological control measures to inhibit the main pathogens(such as Fusarium spp.) that cause ARD is of great significance to the sustainable development of the apple industry. Trichoderma virens 6PS-2, which exhibited antagonism toward a variety of pathogens, was screened from the rhizosphere soils of healthy apple trees(Malus robusta) in different replanted orchards in the Yantai and Zibo Cities, Shandong Province, China. Its fermentation extract inhibited the growth of pathogenic Fusarium proliferatum f. sp. Malus domestica MR5, which was proportional to the concentration. These substances also increased the hairy root volume and growth of Arabidopsis thaliana lateral roots. The phenotype of Malus hupehensis seedlings and microbial community structure in rhizosphere soils in greenhouse experiment using Highthroughput sequencing were analyzed, and the field experiment with grafted apple trees were used for further verification. Compared with the application of potato dextrose broth(PDB) medium, application of 6PS-2 spore suspension directly to replanted soils could improve the growth of M. hupehensis seedlings as well as the elongation of grafted apple trees. Concomitant decreases in the gene copy number of Fusarium and increases in the culturable bacteria/fungi were also observed in the greenhouse and field experiments. The abundance of Trichoderma,Bacillus, and Streptomyces increased significantly, but that of Fusarium, Pseudarthrobacter, and Humicola decreased. The content of esters, phenols,furans, and amino acids in root exudates of M. hupehensis seedlings increased, which significantly inhibited the multiplication of Fusarium, but was positively correlated with Bacillus and Trichoderma. In summary, T. virens 6PS-2 not only directly inhibits the activity of pathogenic Fusarium but also secrets secondary metabolites with antifungal and growth-promoting potential. In addition, 6PS-2 spore suspension can also promote the growth of plants to a certain extent, and change the soil microbial community structure of rhizosphere soils. It is believed that T. virens 6PS-2 has the potential for the alleviation of apple replant disease(ARD) in China.

Biochar alleviates apple replant disease by reducing the growth of Fusarium oxysporum and regulating microbial communities

Apple replant disease(ARD)negatively affects plant growth and reduces yields in replanted orchards.In this study,biochar was applied to apple replant soil with Fusarium oxysporum.Our aim was to investigate whether biochar could promote plant growth and alleviate apple replant disease by reducing the growth of harmful soil microorganisms,changing soil microbial community structure and improving the soil environment.This experiment included five treatments:apple replant soil(CK),methyl bromide fumigation apple replant soil(FM),replant soil with biochar addition(2%),replant soil with F.oxysporum spore solution(8×10^(7)spores·mL^(-1)),and replant soil with biochar and F.oxysporum spore solution addition.Seedling biomass,the activity of antioxidant enzymes in the leaves and roots,and soil environmental variables were measured.Microbial community composition and community structure were analyzed using 16SrDNA and ITS2 gene sequencing.Biochar significantly reduced the abundance of F.oxysporum and increased soil microbial diversity and richness.Biochar also increased the soil enzyme activities(urease,invertase,neutral phosphatase,and catalase),the biomass(plant height,fresh weight,dry weight)and the activity of antioxidant enzymes(superoxide dismutase,peroxidase,and catalase).The root indexes of apple seedlings was also increased in replant soil by biochar.In sum,biochar promoted the growth of plants,improved the replant soil environment,and alleviated apple replant disease.

Potassium alleviated high nitrogen-induced apple growth inhibition by regulating photosynthetic nitrogen allocation and enhancing nitrogen utilization capacity

There is a close relationship between potassium(K)and nitrogen(N).However,the roles of K under high N conditions remain unclear.Using a hydroponics approach,we monitored the morphological,physiological,and molecular changes in M9T337 apple(Malus domestica)rootstocks under different nitrate(10 and 30 mmol·L^(-1)NO_(3)^(-))and K supply(0.5,6,10,and 20 mmol·L_(-1)K^(+))conditions.Results revealed that high nitrate inhibited the root growth of M9T337 rootstocks,downregulated the expressions of K transporter genes(MdPT5,MdHKT1,and MdATK1),and reduced the net NO3-and K+influx at the surface of roots,thereby resulting in an N/K imbalance in rootstocks.Further investigation showed that 10 mmol·L^(-1)K increased the activity of N metabolic enzymes(NR,GS,NiR,and GOGAT),upregulated the expressions of genes related to nitrate uptake and transport(MdNRT1.1,MdNRT1.2,MdNRT1.5,and MdNRT2.4),promoted15N transport from the roots to the shoots,optimized leaf N distribution,and improved photosynthetic N utilization efficiency under high nitrate conditions.These results suggest that the negative effects of high nitrate may be related to the N/K imbalance and that reducing N/K in plants by increasing K supply level can effectively alleviate the inhibition of N assimilation by high nitrate stress.

Improved multi-scale inverse bottleneck residual network based on triplet parallel attention for apple leaf disease identification

Accurate diagnosis of apple leaf diseases is crucial for improving the quality of apple production and promoting the development of the apple industry. However, apple leaf diseases do not differ significantly from image texture and structural information. The difficulties in disease feature extraction in complex backgrounds slow the related research progress. To address the problems, this paper proposes an improved multi-scale inverse bottleneck residual network model based on a triplet parallel attention mechanism, which is built upon ResNet-50, while improving and combining the inception module and ResNext inverse bottleneck blocks, to recognize seven types of apple leaf(including six diseases of alternaria leaf spot, brown spot, grey spot, mosaic, rust, scab, and one healthy). First, the 3×3 convolutions in some of the residual modules are replaced by multi-scale residual convolutions, the convolution kernels of different sizes contained in each branch of the multi-scale convolution are applied to extract feature maps of different sizes, and the outputs of these branches are multi-scale fused by summing to enrich the output features of the images. Second, the global layer-wise dynamic coordinated inverse bottleneck structure is used to reduce the network feature loss. The inverse bottleneck structure makes the image information less lossy when transforming from different dimensional feature spaces. The fusion of multi-scale and layer-wise dynamic coordinated inverse bottlenecks makes the model effectively balances computational efficiency and feature representation capability, and more robust with a combination of horizontal and vertical features in the fine identification of apple leaf diseases. Finally, after each improved module, a triplet parallel attention module is integrated with cross-dimensional interactions among channels through rotations and residual transformations, which improves the parallel search efficiency of important features and the recognition rate of the network with relatively small computational costs while the dimensional dependencies are improved. To verify the validity of the model in this paper, we uniformly enhance apple leaf disease images screened from the public data sets of Plant Village, Baidu Flying Paddle, and the Internet. The final processed image count is 14,000. The ablation study, pre-processing comparison, and method comparison are conducted on the processed datasets. The experimental results demonstrate that the proposed method reaches 98.73% accuracy on the adopted datasets, which is 1.82% higher than the classical ResNet-50 model, and 0.29% better than the apple leaf disease datasets before preprocessing. It also achieves competitive results in apple leaf disease identification compared to some state-ofthe-art methods.

Effect of Different Raw Material Processing Methods on the Quality of Apple Distilled Spirits

In this study,the effect of yeast strains(X16 and RMS2),fruit seed,and pectinase on the quality of apple distilled spirits were investigated with apple as material.The results showed that pectinase shortened the fermentation period.The strain X16,fruit seed remaining,and pectinase addition groups had higher yields of crude distilled spirits than the strain RMS2,fruit seed removal,and without pectinase groups,respectively.Regarding the first-grade distilled spirits quality,the X16 group had higher content of total acids,total esters,higher alcohols,and alcohol content than the RMS2 group;the group with fruit seeds had higher total acids but lower alcohol content and total esters than the group with fruit seed removal;the group with pectinase addition had higher total acids and alcohol content than the group without pectinase addition.Regarding the second-grade distilled spirits quality,the X16 group had higher total acids,total esters,and alcohol content than the RMS2 group;the group without fruit seeds had higher alcohol content than the group with fruit seeds;the group with pectinase addition showcased higher total acids but lower alcohol content than the group without pectinase addition.In summary,yeast strains and pectinase affected the quality of apple distilled spirits,and strain X16 was more suitable for brewing apple distilled spirits.Pectinase affected the quality of apple distilled spirits by affecting fermentation rate and temperature.