In order to develop an efficient method about protein extraction which is suitable for apple proteomic analysis, four protocols of total protein extraction from apple leaves, which are trichloroacetic acid/acetone pre...In order to develop an efficient method about protein extraction which is suitable for apple proteomic analysis, four protocols of total protein extraction from apple leaves, which are trichloroacetic acid/acetone precipitation method （TCA）, phenol extraction methanol/ammonium acetate precipitation method, Tris-HCl extraction method and modified Tris-HCl extraction method were compared. The results showed that the modified Tris-HCl extraction method was the most suitable method in protein extraction for two-dimensional electrophoresis （2-DE） from apple leaves based on the highest resolution and more informative spots of 2-DE gels with no apparent vertical or horizontal streaking.展开更多
In Agriculture Sciences, detection of diseases is one of the mostchallenging tasks. The mis-interpretations of plant diseases often lead towrong pesticide selection, resulting in damage of crops. Hence, the automaticr...In Agriculture Sciences, detection of diseases is one of the mostchallenging tasks. The mis-interpretations of plant diseases often lead towrong pesticide selection, resulting in damage of crops. Hence, the automaticrecognition of the diseases at earlier stages is important as well as economicalfor better quality and quantity of fruits. Computer aided detection (CAD)has proven as a supportive tool for disease detection and classification, thusallowing the identification of diseases and reducing the rate of degradationof fruit quality. In this research work, a model based on convolutional neuralnetwork with 19 convolutional layers has been proposed for effective andaccurate classification of Marsonina Coronaria and Apple Scab diseases fromapple leaves. For this, a database of 50,000 images has been acquired bycollecting images of leaves from apple farms of Himachal Pradesh (H.P)and Uttarakhand (India). An augmentation technique has been performedon the dataset to increase the number of images for increasing the accuracy.The performance analysis of the proposed model has been compared with thenew two Convolutional Neural Network (CNN) models having 8 and 9 layersrespectively. The proposed model has also been compared with the standardmachine learning classifiers like support vector machine, k-Nearest Neighbour, Random Forest and Logistic Regression models. From experimentalresults, it has been observed that the proposed model has outperformed theother CNN based models and machine learning models with an accuracy of99.2%.展开更多
文摘In order to develop an efficient method about protein extraction which is suitable for apple proteomic analysis, four protocols of total protein extraction from apple leaves, which are trichloroacetic acid/acetone precipitation method （TCA）, phenol extraction methanol/ammonium acetate precipitation method, Tris-HCl extraction method and modified Tris-HCl extraction method were compared. The results showed that the modified Tris-HCl extraction method was the most suitable method in protein extraction for two-dimensional electrophoresis （2-DE） from apple leaves based on the highest resolution and more informative spots of 2-DE gels with no apparent vertical or horizontal streaking.
基金This work was supported by Taif University Researchers Supporting Project(TURSP)under number(TURSP-2020/73),Taif University,Taif,Saudi Arabia.
文摘In Agriculture Sciences, detection of diseases is one of the mostchallenging tasks. The mis-interpretations of plant diseases often lead towrong pesticide selection, resulting in damage of crops. Hence, the automaticrecognition of the diseases at earlier stages is important as well as economicalfor better quality and quantity of fruits. Computer aided detection (CAD)has proven as a supportive tool for disease detection and classification, thusallowing the identification of diseases and reducing the rate of degradationof fruit quality. In this research work, a model based on convolutional neuralnetwork with 19 convolutional layers has been proposed for effective andaccurate classification of Marsonina Coronaria and Apple Scab diseases fromapple leaves. For this, a database of 50,000 images has been acquired bycollecting images of leaves from apple farms of Himachal Pradesh (H.P)and Uttarakhand (India). An augmentation technique has been performedon the dataset to increase the number of images for increasing the accuracy.The performance analysis of the proposed model has been compared with thenew two Convolutional Neural Network (CNN) models having 8 and 9 layersrespectively. The proposed model has also been compared with the standardmachine learning classifiers like support vector machine, k-Nearest Neighbour, Random Forest and Logistic Regression models. From experimentalresults, it has been observed that the proposed model has outperformed theother CNN based models and machine learning models with an accuracy of99.2%.
