MK615, a compound extracted from the Japanese apricot "Prunus mume " has been reported to have in vitro anti-tumor activities against several cancer cell lines,including hepatocellular carcinoma(HCC). Howeve...MK615, a compound extracted from the Japanese apricot "Prunus mume " has been reported to have in vitro anti-tumor activities against several cancer cell lines,including hepatocellular carcinoma(HCC). However, the clinical effects and feasibility of administering MK615for patients with HCC were unknown. We experienced a case with advanced HCC for which MK615 was effective against both lymph node and pulmonary metastases. A 60-year-old female underwent surgical resection of a 9 cm HCC in the right lobe. The pathological diagnosis was moderately differentiated HCC with vascular invasion. The HCC recurred in the liver 8 mo after the surgery. Radiofrequency ablation and transarterial infusion chemotherapy were performed, but the recurrence was not controlled. One year after the intrahepatic recurrence, pulmonary and lymph metastasis appeared.Sorafenib was administered, but was not effective.Then, MK615 was administered as a final alternative therapy after informed consent was obtained from the patient. Three months later, her alpha-fetoprotein level decrease and both the lymph node and pulmonary metastases decreased in size. The patient has survived for more than 17 mo after the MK615 administration, and was in good condition. Although further investigations are necessary to clarify its safety and efficacy in humans, MK615 may be useful for the treatment of HCC,without serious adverse effects.展开更多
AIM: To investigate the anti-neoplastic effects of MK615, an extract from the Japanese apricot (Prunus mume), against colon cancer cells. METHODS: Three colon cancer cell lines, SW480, COLO, and WiDr, were cultured wi...AIM: To investigate the anti-neoplastic effects of MK615, an extract from the Japanese apricot (Prunus mume), against colon cancer cells. METHODS: Three colon cancer cell lines, SW480, COLO, and WiDr, were cultured with MK615. Growth inhibition was evaluated by cell proliferation assay and killing activity was determined by lactate dehydrogenase assay. Induction of apoptosis was evaluated by annexin Ⅴ flow cytometry. Morphological changes were studied by light and electron microscopy, and immunofluorescence staining with Atg8. RESULTS: MK615 inhibited growth and lysed SW480, COLO and WiDr cells in a dose-dependent manner. Annexin Ⅴ flow cytometry showed that MK615 induced apoptosis after 6 h incubation, at which point the occurrence of apoptotic cells was 68.0%, 65.7% and 64.7% for SW480, COLO, and WiDr cells, respectively. Light and electron microscopy, and immunofluorescence staining with Atg8 revealed that MK615 induced massive cytoplasmic vacuoles (autophagosomes) in all three cell lines. CONCLUSION: MK615 has an anti-neoplastic effect against colon cancer cells. The effect may be exerted by induction of apoptosis and autophagy.展开更多
[ Objective] This study aimed to investigate the genetic variation between hybrids and their parents, and to find a rapid identification method. [ Method ] The progenies between Prunus armeniaca （ apricot ） Katy and...[ Objective] This study aimed to investigate the genetic variation between hybrids and their parents, and to find a rapid identification method. [ Method ] The progenies between Prunus armeniaca （ apricot ） Katy and Prunus salicina （plum） Moriteni were analyzed by means of RAPD and SSR molecular markers. [ Result] We found the differences in the electrophoresis bands between progenies and their parents, indicating that hybrid seedlings obtained in this study were real. [ Conclusion ] The combination of the above methods is effective and fast to identify the hybrids, and can shorten the breeding period.展开更多
Several groups in the USA have been working to de-velop a low chill apricot that would ripen its fruits veryearly in the season. Unfortunately, the cultivatcd apricothas been developed mainly from the high chilling Eu...Several groups in the USA have been working to de-velop a low chill apricot that would ripen its fruits veryearly in the season. Unfortunately, the cultivatcd apricothas been developed mainly from the high chilling Euro-pean group within the species Prunus armeniaca. Amongthe five groups within the apricot species, this is the mostrecently evolved and least variable (Bailey and展开更多
探讨了在编著《中国梅花品种图志.中英双语增订版》时,为解决与国际接轨而遇到的先决问题。经钻研《国际栽培植物命名法规》(第7版)后,在梅种之下将品种分为3个品种群,即:①真梅品种群True Mume Group,②杏梅品种群Apricot Mei Group,...探讨了在编著《中国梅花品种图志.中英双语增订版》时,为解决与国际接轨而遇到的先决问题。经钻研《国际栽培植物命名法规》(第7版)后,在梅种之下将品种分为3个品种群,即:①真梅品种群True Mume Group,②杏梅品种群Apricot Mei Group,③樱李梅品种群Blireiana Group。品种群以下,则不再分类、型等级别。而将二元分类法的内涵,融入品种名称之中。不同意樱李梅用独立的杂种学名Prunus×blireiana,也不同意把杏梅提升为独立的种名Prunus×bungo。樱李梅和杏梅还都应列在梅种之下,因三者之内果皮表面均具蜂窝状点穴——此乃梅、杏、李鉴别分类中的最关键性状,必须首先考虑,不能与其他性状等量齐观。展开更多
Embryo abortion stage and rescue system of hybrids were studied in the distant hybridizationbetween plum and apricot. Identification of the hybrids was also made. The resultsshowed: (1) Embryo abortion started from th...Embryo abortion stage and rescue system of hybrids were studied in the distant hybridizationbetween plum and apricot. Identification of the hybrids was also made. The resultsshowed: (1) Embryo abortion started from three weeks after pollination. (2) The germinationand growth of embryos were different at different growth stages, which could germinateand grow with PF value>0.5, but failed with PF value<0.5. In embryo rescue system ofhybrids, the best germination and differentiation medium was MS+6-BA 2mgL-1+IAA 0.3mgL-1,the rate of germination and differentiation reached up to 80%, bud induction andmultiplication medium was MS+6-BA 1.5mgL-1+IAA 0.3mgL-1, rooting medium was 1/2 MS+IAA0.8mgL-1. Some hybrids were transplanted into the field successfully. (3) Leaf shapeinvestigation and identification by S allele-specific PCR and RAPDs showed that thehybrids were true ones.展开更多
文摘MK615, a compound extracted from the Japanese apricot "Prunus mume " has been reported to have in vitro anti-tumor activities against several cancer cell lines,including hepatocellular carcinoma(HCC). However, the clinical effects and feasibility of administering MK615for patients with HCC were unknown. We experienced a case with advanced HCC for which MK615 was effective against both lymph node and pulmonary metastases. A 60-year-old female underwent surgical resection of a 9 cm HCC in the right lobe. The pathological diagnosis was moderately differentiated HCC with vascular invasion. The HCC recurred in the liver 8 mo after the surgery. Radiofrequency ablation and transarterial infusion chemotherapy were performed, but the recurrence was not controlled. One year after the intrahepatic recurrence, pulmonary and lymph metastasis appeared.Sorafenib was administered, but was not effective.Then, MK615 was administered as a final alternative therapy after informed consent was obtained from the patient. Three months later, her alpha-fetoprotein level decrease and both the lymph node and pulmonary metastases decreased in size. The patient has survived for more than 17 mo after the MK615 administration, and was in good condition. Although further investigations are necessary to clarify its safety and efficacy in humans, MK615 may be useful for the treatment of HCC,without serious adverse effects.
文摘AIM: To investigate the anti-neoplastic effects of MK615, an extract from the Japanese apricot (Prunus mume), against colon cancer cells. METHODS: Three colon cancer cell lines, SW480, COLO, and WiDr, were cultured with MK615. Growth inhibition was evaluated by cell proliferation assay and killing activity was determined by lactate dehydrogenase assay. Induction of apoptosis was evaluated by annexin Ⅴ flow cytometry. Morphological changes were studied by light and electron microscopy, and immunofluorescence staining with Atg8. RESULTS: MK615 inhibited growth and lysed SW480, COLO and WiDr cells in a dose-dependent manner. Annexin Ⅴ flow cytometry showed that MK615 induced apoptosis after 6 h incubation, at which point the occurrence of apoptotic cells was 68.0%, 65.7% and 64.7% for SW480, COLO, and WiDr cells, respectively. Light and electron microscopy, and immunofluorescence staining with Atg8 revealed that MK615 induced massive cytoplasmic vacuoles (autophagosomes) in all three cell lines. CONCLUSION: MK615 has an anti-neoplastic effect against colon cancer cells. The effect may be exerted by induction of apoptosis and autophagy.
基金Supported by the Natural Science Foundation of Shandong Province(Q2008D01)Program for Science Research Development of Higher Education Institutions of Shandong Province(J09LC54)
文摘[ Objective] This study aimed to investigate the genetic variation between hybrids and their parents, and to find a rapid identification method. [ Method ] The progenies between Prunus armeniaca （ apricot ） Katy and Prunus salicina （plum） Moriteni were analyzed by means of RAPD and SSR molecular markers. [ Result] We found the differences in the electrophoresis bands between progenies and their parents, indicating that hybrid seedlings obtained in this study were real. [ Conclusion ] The combination of the above methods is effective and fast to identify the hybrids, and can shorten the breeding period.
文摘Several groups in the USA have been working to de-velop a low chill apricot that would ripen its fruits veryearly in the season. Unfortunately, the cultivatcd apricothas been developed mainly from the high chilling Euro-pean group within the species Prunus armeniaca. Amongthe five groups within the apricot species, this is the mostrecently evolved and least variable (Bailey and
基金supported by the Technological Production Transformation Foundation by the Ministry of Science and Technology of China(2002370010495)the foundation of Shandong Fruit Tree Three-Zero Project
文摘Embryo abortion stage and rescue system of hybrids were studied in the distant hybridizationbetween plum and apricot. Identification of the hybrids was also made. The resultsshowed: (1) Embryo abortion started from three weeks after pollination. (2) The germinationand growth of embryos were different at different growth stages, which could germinateand grow with PF value>0.5, but failed with PF value<0.5. In embryo rescue system ofhybrids, the best germination and differentiation medium was MS+6-BA 2mgL-1+IAA 0.3mgL-1,the rate of germination and differentiation reached up to 80%, bud induction andmultiplication medium was MS+6-BA 1.5mgL-1+IAA 0.3mgL-1, rooting medium was 1/2 MS+IAA0.8mgL-1. Some hybrids were transplanted into the field successfully. (3) Leaf shapeinvestigation and identification by S allele-specific PCR and RAPDs showed that thehybrids were true ones.
