In the last decade,a new neurological disease concept known as anti-myelin oligodendrocyte glycoprotein antibody(MOG-IgG)-associated disease(MOGAD)has emerged and is currently one of the most focused research areas in...In the last decade,a new neurological disease concept known as anti-myelin oligodendrocyte glycoprotein antibody(MOG-IgG)-associated disease(MOGAD)has emerged and is currently one of the most focused research areas in the field of neuroimmunology.MOG is a membrane protein mainly expressed on the surface of oligodendrocytes(Zhou et al.,2006).The exact pathogenic role of MOG-IgG in patients with MOGAD remains unclear;however,MOG-IgG has been suggested to cause tissue alterations and damage MOG-expressing cells(Zhou et al.,2006).The pathogenicity of MOG-IgG is further supported by the observation that only a few patients with acquired central nervous system(CNS)demyelinating syndromes exhibit both anti-aquaporin-4 antibody(AQP4-IgG)and MOG-IgG simultaneously,particularly with clear positivity levels of these antibodies as indicated by a cell-based assay result with a titer≥1:100(Sechi et al.,2021;Banwell et al.,2023).展开更多
Compared to other organisms,plants have evolved a greater number of aquaporins with diverse substrates and functions to adapt to ever-changing environmental and internal stimuli for growth and development.Although aqu...Compared to other organisms,plants have evolved a greater number of aquaporins with diverse substrates and functions to adapt to ever-changing environmental and internal stimuli for growth and development.Although aquaporins were initially identified as channels that allow water molecules to cross biological membranes,progress has been made in identifying various novel permeable substrates.Many studies have characterized the versatile physiological and biophysical functions of plant aquaporins.Here,we review the recent reports that highlight aquaporin-facilitated regulation of major physiological processes and stress tolerance throughout plant life cycles as well as the potential prospects and possibilities of applying aquaporins to improve agricultural productivity,food quality,environmental protection,and ecological conservation.展开更多
Plasma membrane intrinsic proteins(PIPs)are conserved plant aquaporins that transport small molecules across the plasma membrane to trigger instant stress responses and maintain cellular homeostasis under biotic and a...Plasma membrane intrinsic proteins(PIPs)are conserved plant aquaporins that transport small molecules across the plasma membrane to trigger instant stress responses and maintain cellular homeostasis under biotic and abiotic stress.To elucidate their roles in plant immunity to pathogen attack,we characterized the expression patterns,subcellular localizations,and H_(2)O_(2)-transport ability of 11 OsPIPs in rice(Oryza sativa),and identified OsPIP2;6 as necessary for rice disease resistance.OsPIP2;6 resides on the plasma membrane and facilitates cytoplasmic import of the immune signaling molecule H_(2)O_(2).Knockout of OsPIP2;6 increases rice susceptibility to Magnaporthe oryzae,indicating a positive function in plant immunity.OsPIP2;6 interacts with OsPIP2;2,which has been reported to increase rice resistance to pathogens via H_(2)O_(2)transport.Our findings suggest that OsPIP2;6 cooperates with OsPIP2;2 as a defense signal transporter complex during plant–pathogen interaction.展开更多
目的探讨微肽抑制肌动蛋白细胞骨架(MIAC)和水通道蛋白2(AQP2)与宫颈癌症临床病理特征的关系。方法选取2021年1月至2022年1月在上海市第一妇婴保健院妇科收治的100例宫颈癌患者为研究对象。所有患者接受根治性子宫颈切除术,收集肿瘤组...目的探讨微肽抑制肌动蛋白细胞骨架(MIAC)和水通道蛋白2(AQP2)与宫颈癌症临床病理特征的关系。方法选取2021年1月至2022年1月在上海市第一妇婴保健院妇科收治的100例宫颈癌患者为研究对象。所有患者接受根治性子宫颈切除术,收集肿瘤组织及肿瘤周围组织用于蛋白和免疫组化分析。根据肿瘤分期分析MIAC和AQP2表达,绘制Kaplan-Meier生存曲线,比较不同MIAC和AQP2表达组间的中位生存时间。通过免疫组化分析癌旁组织和宫颈癌组织中MIAC和AQP2的表达。通过免疫共沉淀实验分析MIAC与AQP2的相互作用。结果宫颈癌组织中MIAC与AQP2的蛋白表达较癌旁组织降低(MIAC:1.02±0.02 vs 1.85±0.16;AQP2:1.09±0.04 vs 1.75±0.14,P<0.05)。染色分析显示宫颈癌组织中MIAC与AQP2的蛋白表达较癌旁组织降低(MIAC:2.49±0.67 vs 6.33±1.26;AQP2:3.26±0.49 vs 7.49±1.15,P<0.05)。Ⅲ~Ⅳ病期组患者MIAC和AQP2蛋白表达较Ⅰ~Ⅱ病期组降低(P<0.05),随着宫颈癌病期的进展,MIAC和AQP2的表达水平降低差异有统计学意义(P<0.05)。Kaplan-Meier生存分析显示高MIAC表达的患者群体在整个时间范围内的生存率普遍高于低MIAC表达的患者(P<0.05),高MIAC表达与更好的生存预后相关,而低MIAC表达的患者生存率较低。与非特异性的IgG对照组相比,使用针对MIAC和AQP2的特异性抗体时,AQP2和MIAC蛋白表达升高(P<0.05),表明MIAC与AQP2之间存在相互作用(MIAC IP为1.75±0.16;AQP2 IP为1.86±0.15;IgG对照为1.04±0.02)。结论宫颈癌中微肽MIAC与AQP2的相互作用对肿瘤的发展具有重要影响。这两种蛋白的表达水平显著降低与病情的恶化相关,而高MIAC表达与更佳的生存预后相关,揭示了它们在宫颈癌进展中的关键作用。展开更多
文摘In the last decade,a new neurological disease concept known as anti-myelin oligodendrocyte glycoprotein antibody(MOG-IgG)-associated disease(MOGAD)has emerged and is currently one of the most focused research areas in the field of neuroimmunology.MOG is a membrane protein mainly expressed on the surface of oligodendrocytes(Zhou et al.,2006).The exact pathogenic role of MOG-IgG in patients with MOGAD remains unclear;however,MOG-IgG has been suggested to cause tissue alterations and damage MOG-expressing cells(Zhou et al.,2006).The pathogenicity of MOG-IgG is further supported by the observation that only a few patients with acquired central nervous system(CNS)demyelinating syndromes exhibit both anti-aquaporin-4 antibody(AQP4-IgG)and MOG-IgG simultaneously,particularly with clear positivity levels of these antibodies as indicated by a cell-based assay result with a titer≥1:100(Sechi et al.,2021;Banwell et al.,2023).
基金supported by the National Key Research and Development Program of China(2021YFD1201104-02)National Natural Science Foundation of China(32272048)to Xiaodong Ding。
文摘Compared to other organisms,plants have evolved a greater number of aquaporins with diverse substrates and functions to adapt to ever-changing environmental and internal stimuli for growth and development.Although aquaporins were initially identified as channels that allow water molecules to cross biological membranes,progress has been made in identifying various novel permeable substrates.Many studies have characterized the versatile physiological and biophysical functions of plant aquaporins.Here,we review the recent reports that highlight aquaporin-facilitated regulation of major physiological processes and stress tolerance throughout plant life cycles as well as the potential prospects and possibilities of applying aquaporins to improve agricultural productivity,food quality,environmental protection,and ecological conservation.
基金supported by the Guangdong Basic and Applied Basic Research Foundation(2020A1515111101,2022A1515110431).
文摘Plasma membrane intrinsic proteins(PIPs)are conserved plant aquaporins that transport small molecules across the plasma membrane to trigger instant stress responses and maintain cellular homeostasis under biotic and abiotic stress.To elucidate their roles in plant immunity to pathogen attack,we characterized the expression patterns,subcellular localizations,and H_(2)O_(2)-transport ability of 11 OsPIPs in rice(Oryza sativa),and identified OsPIP2;6 as necessary for rice disease resistance.OsPIP2;6 resides on the plasma membrane and facilitates cytoplasmic import of the immune signaling molecule H_(2)O_(2).Knockout of OsPIP2;6 increases rice susceptibility to Magnaporthe oryzae,indicating a positive function in plant immunity.OsPIP2;6 interacts with OsPIP2;2,which has been reported to increase rice resistance to pathogens via H_(2)O_(2)transport.Our findings suggest that OsPIP2;6 cooperates with OsPIP2;2 as a defense signal transporter complex during plant–pathogen interaction.
文摘目的探讨微肽抑制肌动蛋白细胞骨架(MIAC)和水通道蛋白2(AQP2)与宫颈癌症临床病理特征的关系。方法选取2021年1月至2022年1月在上海市第一妇婴保健院妇科收治的100例宫颈癌患者为研究对象。所有患者接受根治性子宫颈切除术,收集肿瘤组织及肿瘤周围组织用于蛋白和免疫组化分析。根据肿瘤分期分析MIAC和AQP2表达,绘制Kaplan-Meier生存曲线,比较不同MIAC和AQP2表达组间的中位生存时间。通过免疫组化分析癌旁组织和宫颈癌组织中MIAC和AQP2的表达。通过免疫共沉淀实验分析MIAC与AQP2的相互作用。结果宫颈癌组织中MIAC与AQP2的蛋白表达较癌旁组织降低(MIAC:1.02±0.02 vs 1.85±0.16;AQP2:1.09±0.04 vs 1.75±0.14,P<0.05)。染色分析显示宫颈癌组织中MIAC与AQP2的蛋白表达较癌旁组织降低(MIAC:2.49±0.67 vs 6.33±1.26;AQP2:3.26±0.49 vs 7.49±1.15,P<0.05)。Ⅲ~Ⅳ病期组患者MIAC和AQP2蛋白表达较Ⅰ~Ⅱ病期组降低(P<0.05),随着宫颈癌病期的进展,MIAC和AQP2的表达水平降低差异有统计学意义(P<0.05)。Kaplan-Meier生存分析显示高MIAC表达的患者群体在整个时间范围内的生存率普遍高于低MIAC表达的患者(P<0.05),高MIAC表达与更好的生存预后相关,而低MIAC表达的患者生存率较低。与非特异性的IgG对照组相比,使用针对MIAC和AQP2的特异性抗体时,AQP2和MIAC蛋白表达升高(P<0.05),表明MIAC与AQP2之间存在相互作用(MIAC IP为1.75±0.16;AQP2 IP为1.86±0.15;IgG对照为1.04±0.02)。结论宫颈癌中微肽MIAC与AQP2的相互作用对肿瘤的发展具有重要影响。这两种蛋白的表达水平显著降低与病情的恶化相关,而高MIAC表达与更佳的生存预后相关,揭示了它们在宫颈癌进展中的关键作用。
