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Response Surface Optimization of Ultrasound-assisted Aqueous Two-phase Extraction of Sweet Potato Leaf Polysaccharides
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作者 Yuxuan WU Junqing WANG Wenjie WANG 《Agricultural Biotechnology》 CAS 2023年第3期106-112,共7页
[Objectives]The ultrasound-assisted aqueous two-phase extraction of sweet potato leaf polysaccharides was studied.[Methods]With the yield of sweet potato leaf polysaccharides as the index,the aqueous two-phase extract... [Objectives]The ultrasound-assisted aqueous two-phase extraction of sweet potato leaf polysaccharides was studied.[Methods]With the yield of sweet potato leaf polysaccharides as the index,the aqueous two-phase extraction system was determined,and the optimal extraction conditions were optimized by single-factor experiments and response surface methodology.[Results]The optimal parameters were ethanol concentration 25.68%,liquid-to-material ratio 55.83,and ultrasonic treatment time 38.33 min.Under these conditions,the yield of sweet potato leaf polysaccharides could reach 20.646 mg/g.[Conclusions]The ethanol/ammonium sulfate aqueous system is a rapid and efficient method for extracting sweet potato leaf polysaccharides,which is of great significance for the application of sweet potato leaf extract as a natural food additive. 展开更多
关键词 aqueous two-phase system Ultrasound assistance Response surface optimization Sweet potato leaf polysaccharides
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Simultaneous Cell Disruption and Aqueous Two-Phase Extraction for Isolation of Intracellular Recombinant Proteins 被引量:3
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作者 王光虎 冯小黎 苏志国 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 1999年第2期139-144,共6页
A new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins from microbial cells. The process was named ... A new technique was developed for the integrated processing of cell disruption and aqueous two-phase extraction in a high-speed bead mill to separate intracellular proteins from microbial cells. The process was named as simultaneous cell disruption and aqueous two-phase extraction (SDATE). Advantages, such as high cell disruption efficiency, biochemical activities preservation of proteins, cell debris elimination, and prelimiary purification of the target protein were being claimed. When this technique was employed for isolating recombinant Tumor Necrosis Factor (TNF) from E. coli, overall protein concentration and TNF activity were found to have been increased. More than 95% of TNF was partitioned into the top phase and all cell debris were in the bottom phase. The partition coefficient was greater than 3 and the TNF purification factor was greater than 6. It is shown that less separation steps were being utilized in the new technique, meaning a reduction in separation time and less process extractors required. 展开更多
关键词 SDATE release of intracellular proteins high speed bead mill aqueous two-phase extraction tumor necrosis factor
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Selective separation and enrichment of proteins in aqueous two-phase extraction system
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作者 Feng Qu Hao Qin +3 位作者 Min Dong Dong Xu Zhao Xin Ying Zhao Jing Hua Zhang 《Chinese Chemical Letters》 SCIE CAS CSCD 2009年第9期1100-1102,共3页
A simple aqueous two-phase extraction system (ATPS) of PEG/phosphate was proposed for selective separation and enrichment of proteins. The combination of ATPE with HPLC was applied to identify the partition of prote... A simple aqueous two-phase extraction system (ATPS) of PEG/phosphate was proposed for selective separation and enrichment of proteins. The combination of ATPE with HPLC was applied to identify the partition of proteins in two phases. Five proteins (bovine serum albumin, Cytochrome C, lysozyme, myoglobin, and trypsin) were used as model proteins to study the effect of phosphate concentration and pH on proteins partition. The PEG/phosphate system was firstly applied to real human saliva and plasma samples, some proteins showed obviously different partition in two phases. The primary results manifest the selective separation and enrichment of proteins in ATPS provided the potential for high abundance proteins depletion in proteomics. ~ 2009 Feng Qu. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved. 展开更多
关键词 aqueous two-phase extraction Saliva and plasma PROTEINS Selective separation and enrichment
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Extraction of Theanine from Waste Liquid of Tea Polyphenol Production in Aqueous Two-phase Systems with Cationic and Anionic Surfactants 被引量:8
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作者 张军伟 王艳 彭奇均 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2013年第1期31-36,共6页
Extraction of theanine from waste liquid of tea polyphenol production was studied in aqueous surfactanttwo-phase system (ASTP) with cationic surfactant (CTAB) and anionic surfactant (SDS). Results indicate that ... Extraction of theanine from waste liquid of tea polyphenol production was studied in aqueous surfactanttwo-phase system (ASTP) with cationic surfactant (CTAB) and anionic surfactant (SDS). Results indicate that the region of ASTP is narrow and there is only a two-phase region of cationic surfactant. The increase in concentrations of NaBr and Na2SO4 are beneficial to the formation of ASTP. Theanine concentration in the bottom phase increases with increasing concentration of theanine, whereas the Partition coefficient and extraction rate only change a littlewhen the concentration of theanine is above 0.2 g.L-'. With the increase of SDS concentration, the phase ratio and the partition coefficient decrease, while the extraction efficiency of theanine increases and the concentration of theanine changes a little in the range from 2.4/7.5 to 2.8/7.2 for SDS/CTAB ratio. The temperature has a notable ef- fect on the concentration of theanine in the bottom phase, partition coefficient and extraction rate of theanine. The increase of waste liquid decreases the phase ratio, increases the concentration and extraction rate of theanine in the bottom ohase, since the orotein and the saccharide enter the bottom nhase with theanine. 展开更多
关键词 aqueous two-phase system SURFACTANT THEANINE waste liquid of tea polyphenol production extraction
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Ionic Liquid-salt Aqueous Two-phase System, a Novel System for theExtraction of Abused Drugs 被引量:4
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作者 She Hong LI Chi Yang HE +2 位作者 Hu Wei LIU Ke An LI Feng LIU 《Chinese Chemical Letters》 SCIE CAS CSCD 2005年第8期1074-1076,共3页
A 1-butyl-3-methylimidazolium chloride-salt aqueous two-phase system was studied on extraction of abused drugs. The effects of sorts of salts, temperature, concentration of salt and drugs on system were investigated s... A 1-butyl-3-methylimidazolium chloride-salt aqueous two-phase system was studied on extraction of abused drugs. The effects of sorts of salts, temperature, concentration of salt and drugs on system were investigated systematically. A satisfactory extraction efficiency of 93% was obtained for papaverine while that of morphine was 65%. The extraction mechanism was primarily discussed. 展开更多
关键词 aqueous two-phase system ionic liquids 1-butyl-3-methylimidazolium chloride extraction abused drugs.
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Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system 被引量:6
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作者 徐莹 何国庆 李景军 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE EI CAS CSCD 2005年第11期1087-1094,共8页
This paper presents the evaluation of an aqueous two-phase system (ATPS) for extracting elastase produced by Bacillus sp. EL31410. The elastase and cell partition behavior in polyethylene glycol (PEG)/salt systems... This paper presents the evaluation of an aqueous two-phase system (ATPS) for extracting elastase produced by Bacillus sp. EL31410. The elastase and cell partition behavior in polyethylene glycol (PEG)/salt systems was investigated. The suitable system for elastase extraction was PEG/KHEPO4-KEHPO4, in which elastase is mainly partitioned into the PEG-rich phase, while the cells remained in the other phase. The influence of defined system parameters (e.g. PEG molecular mass, pH, NaCl addition) on the partitioning behavior of elastase is described. The concentration of phase forming components, PEG and KHEPO4-KEHPO4, was optimized for elastase recovery by means of response surface methodology, and it was found that they greatly influenced extraction recovery. The optimal ATPS was 23.1% (w/w) PEG 2000 and 11.7% (w/w) KHEPO4-KEHPO4. The predicted recovery was about 89.5%, so this process is suggested to be a rapid and convenient method for elastase extraction. 展开更多
关键词 ELASTASE aqueous two-phase system (ATPS) BIOSEPARATION PURIFICATION Optimization Response surface methodology (RSM)
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Characterisation and separation of infectious bursal disease virus-like particles using aqueous two-phase systems
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作者 Hui Yi Leong Xiao-Qian Fu +2 位作者 Xiang-Yu Liu Shan-Jing Yao Dong-Qiang Lin 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2023年第5期72-78,共7页
Infectious bursal disease(IBD)causes considerable economic losses in the commercial poultry industry worldwide.The principal way to control IBD virus(IBDV),the causative agent of IBD,is still through vaccination progr... Infectious bursal disease(IBD)causes considerable economic losses in the commercial poultry industry worldwide.The principal way to control IBD virus(IBDV),the causative agent of IBD,is still through vaccination programs.Virus-like particles(VLPs)are recognised as a safe and potent recombinant vaccine platform.This research work explores the characterisation and separation of infectious bursal disease virus-like particles(IBD-VLPs)from crude feedstock.Various characteristics were studied with highperformance size-exclusion chromatography(HP-SEC),sodium dodecyl sulphate–polyacrylamide gel electrophoresis(SDS-PAGE)and transmission electron microscopy(TEM)analyses.Subsequently,the separation of IBD-VLPs using polyethylene glycol(PEG)/sodium citrate-based aqueous two-phase systems(ATPSs)was conducted and optimised.Moreover,a scale-up study of the best ATPS constituted of 15%PEG 6000,11%sodium citrate and 10%crude feedstock was performed to compare the separation performance of IBD-VLPs with and without centrifugation-assisted.The results indicated that the optimised ATPS with centrifugation-assisted for both 5 g and 50 g systems showed good recovery of IBDVLPs of>97%in the interphase between the PEG-rich top and salt-rich bottom phases.These optimised systems also showed high removal efficiencies of impurities of>95%.The results demonstrated that aqueous two-phase extraction could be a promising technology for efficient VLPs separation. 展开更多
关键词 aqueous two-phase extraction Infectious bursal disease virus POLYMERS SALT SEPARATION Virus-like particle
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Measurement of Mass Transfer Coefficients and Their Modelling of Continuous Counter-Current Aqueous Two-Phase System in a Packed Extraction Column 被引量:1
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作者 阎恭喜 朱自强 +1 位作者 关怡新 韩兆熊 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 1999年第3期196-204,共9页
Overall dispersed side volumetric mass transfer coefficients for protein and amino acids were measured in continuous countercurrent PEG4000/KHP aqueous two-phase systems in a 57mm I.D. packed extraction column. A mode... Overall dispersed side volumetric mass transfer coefficients for protein and amino acids were measured in continuous countercurrent PEG4000/KHP aqueous two-phase systems in a 57mm I.D. packed extraction column. A model for overall dispersed side volumetric mass transfer coefficients was derived by describing the motion of the drops based upon Navier-Stokes equation combined with the relationship between mass transfer coefficients and the drop velocity. The model provides good predictions and can be successfully used in aqueous two-phase extraction. The average relative deviation between calculated values and experimental data ranges from 8% to 14%. 展开更多
关键词 aqueous two-phase system continuous countercurrent extraction mass transfer coefficient MODEL packed column
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Bench-top aqueous two-phase extraction of isolated individual single-walled carbon nanotubes 被引量:2
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作者 Navaneetha K. Subbaiyan A. Nicholas G. Parra-Vasquezt +7 位作者 Sofie Cambre Miguel A. Santiago Cordoba Sibel Ebru Yalcin Christopher E. Hamilton Nathan H. Mack Jeffrey L. Blackburn Stephen K. Doorn Juan G. Duque 《Nano Research》 SCIE EI CAS CSCD 2015年第5期1755-1769,共15页
Isolation and purification of single-walled carbon nanotubes (SWCNTs) are prerequisites for their implementation in various applications. In this work, we present a fast (-5 min), low-cost, and easily scalable ben... Isolation and purification of single-walled carbon nanotubes (SWCNTs) are prerequisites for their implementation in various applications. In this work, we present a fast (-5 min), low-cost, and easily scalable bench-top approach to the extraction of high-quality isolated SWCNTs from bundles and impurities in an aqueous dispersion. The extraction procedure, based on aqueous two-phase (ATP) separation, is widely applicable to any SWCNT source (tested on samples up to 1.7 nm in diameter) and independent of defect density, purity, diameter, and length. The extracted dispersions demonstrate that the removal of large aggregates, small bundles, and impurities is comparable to that by density gradient ultracentrifugation, but without the need for high-end instrumentation. Raman and fluorescence-excitation spectroscopy, single-nanotube fluorescence imaging, atomic force and transmission electron microscopy, and thermogravimetric analysis all confirm the high purity of the isolated SWCNTs. By predispersing the SWCNTs without sonication (only gentle stirring), full-length, pristine SWCNTs can be isolated (tested up to 20 μm). Hence, this simple ATP method will find immediate application in the generation of SWCNT materials for all levels of nanotube research and applications, from fundamental studies to high-performance devices. 展开更多
关键词 carbon nanotubes aqueous two-phase (ATP)separation aggregate removal isolation SORTING
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EXTRACTION OF INTERFERON-γ USING PEG/K_3PO_4 AQUEOUS TWO-PHASE SYSTEM
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作者 沈忠耀 丛梅 杨基础 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 1993年第3期48-51,共4页
The aqueous two-phase system of PEG-4000/K<sub>3</sub>PO<sub>4</sub> is selected to extract interferon-γ(γ-IFN)from the broth of homogenized E.coil cells,which were recombined genetically a... The aqueous two-phase system of PEG-4000/K<sub>3</sub>PO<sub>4</sub> is selected to extract interferon-γ(γ-IFN)from the broth of homogenized E.coil cells,which were recombined genetically and werefermented in high expressivity.The effects of pH value and the concentrations of PEG and phos-phate on the partition behavious of γ-IFN and contaminating proteins have been investigated.Theresults show that the pH value is the most influential factor effecting the extraction yield of γ-IFN.Under a suitable condition,an effective separation with high yield of γ-IFN can be achieved. 展开更多
关键词 aqueous two-phase extraction INTERFERON-Γ
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Phytochemical Analysis and Antioxidant Activity of Aqueous and Hydroethanolic Extracts from Three Anticancerous Fabaceae of Northern Cameroon Pharmacopoeia
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作者 Halmata Mohamadou Landry Lienou Lienou +5 位作者 Richard Simo Tagne Herve Pabamé Kada Elisee Libert Enyengue Embolo Grace Kembaou Nganwa Jean Louis Oyono Essame Piere Michel Dongmo Jazet 《Journal of Biosciences and Medicines》 2024年第6期19-32,共14页
Background: Cancer continues to pose a significant threat to our society, representing one of the most pressing health concerns worldwide. This study aimed to evaluate the chemical composition and the antioxidant acti... Background: Cancer continues to pose a significant threat to our society, representing one of the most pressing health concerns worldwide. This study aimed to evaluate the chemical composition and the antioxidant activity of aqueous and hydroethanolic extracts from Acacia nilotica (An), Bauhinia reticulate (Br), and Tamarindus indica (Ti) of Fabaceae family, traditionally used in Northern Cameroon for cancer treatment. Methods: The phytochemical screening of the three plants was conducted using conventional colorimetric methods, followed by the measurement of total phenol content, flavonoids, and tannins. The antiradical and antioxidant activities of both plant extracts were assessed through FRAP, ABTS, and DPPH methods. A principal components analysis was employed to correlate the quantities of the evaluated secondary metabolites with the activities. Results: Both types of extracts from the three plants contain alkaloids, saponins, flavonoids, phenolic compounds, tannins, glycosides, terpenoids, coumarins, anthocyanins, and anthraquinones. The aqueous extracts of Br and An are significantly richer (p Conclusion: The three Fabaceae plants from northern Cameroon, prepared in different solvents, can be utilized for their antiradical properties in cancer treatment. 展开更多
关键词 Cancer ANTIOXIDANT FABACEAE Phenolic Compounds aqueous extract Hydroethanolic extract
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Liquid–liquid extraction of levulinic acid from aqueous solutions using hydrophobic tri-n-octylamine/alcohol-based deep eutectic solvent
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作者 Yinglin Mai Xiaoling Xian +4 位作者 Lei Hu Xiaodong Zhang Xiaojie Zheng Shunhui Tao Xiaoqing Lin 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2023年第2期248-256,共9页
Levulinic acid(LA)is one of the top-12 most promising biomass-based platform chemicals,which has a wide range of applications in a variety of fields.However,separation and purification of LA from aqueous solution or a... Levulinic acid(LA)is one of the top-12 most promising biomass-based platform chemicals,which has a wide range of applications in a variety of fields.However,separation and purification of LA from aqueous solution or actual hydrolysate continues to be a challenge.Among various downstream separation technologies,liquid-liquid extraction is a low-cost,effective,and simple process to separate LA.The key breakthrough lies in the development of extractants with high extraction efficiency,good hydrophobicity,and low cost.In this work,three hydrophobic deep eutectic solvents(DESs)based on tri-n-octylamine(TOA)as hydrogen bond acceptor(HBA)and alcohols(butanol,2-octanol,and menthol)as hydrogen bond donors(HBDs)were developed to extract LA from aqueous solution.The molar ratios of HBD and HBA,extraction temperature,contact time,solution pH,and initial LA concentration,DES/water volume ratios were systematically investigated.Compared with 2-octanol-TOA and menthol-TOA DES,the butanol-TOA DES exhibited the superior extraction performance for LA,with a maximum extraction efficiency of 95.79±1.4%.Moreover,the solution pH had a great impact on the LA extraction efficiency of butanol-TOA(molar ratio=3:1).It is worth noting that the extraction equilibrium time was less than 0.5 h.More importantly,the butanol-TOA(3:1)DES possesses good extraction abilities for low,medium,and high concentrations of LA. 展开更多
关键词 extraction SEPARATION Levulinic acid Deep eutectic solvents HYDROPHOBIC aqueous solution
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Extraction of Tetracycline via Ionic Liquid Two-phase System
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作者 MA Chun-hong WANG Liang +4 位作者 YAN Yong-sheng CHE Guang-bo YIN Yan-su WANG Ren-zhang LI Dong-ying 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2009年第6期832-835,共4页
A new method to extract and separate tetracycline(TC) in a new aqueous tyro-phase system(ATPS) consisting of hydrophilic [Bmim]BF4 and NaH2PO4 was investigated. The effects of the amount of salt, ionic liquid as w... A new method to extract and separate tetracycline(TC) in a new aqueous tyro-phase system(ATPS) consisting of hydrophilic [Bmim]BF4 and NaH2PO4 was investigated. The effects of the amount of salt, ionic liquid as well as tetracycline on extraction efficiency were studied systematically. The results show that ionic liquid-ATPS exhibited the highest extraction efficiency higher than 90%. When the concentration of NaH2PO4 was 33.3%--38.5% (mass fraction), the amounts of ionic liquid and TC were 1.0--2.0 mL and 1.8 mL, respectively, and ionic liquid could be re-used. 展开更多
关键词 Ionic liquid aqueous two-phase TETRACYCLINE extraction separation
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Mori fructus aqueous extracts attenuates liver injury by inhibiting ferroptosis via the Nrf2 pathway 被引量:4
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作者 Yuanyuan Wei Chen Gao +5 位作者 Huiru Wang Yannan Zhang Jinhua Gu Xiuying Zhang Xuhao Gong Zhihui Hao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第4期1418-1437,共20页
Background Liver fibrosis and hepatocellular carcinogenesis secondary to liver fibrosis are serious liver diseases with no effective treatments.Mori fructus aqueous extracts(MFAEs)have served as successful treatments ... Background Liver fibrosis and hepatocellular carcinogenesis secondary to liver fibrosis are serious liver diseases with no effective treatments.Mori fructus aqueous extracts(MFAEs)have served as successful treatments for many types of liver injury including fibrosis although the molecular mechanisms are unknown at present.Purpose To investigate the effect of MFAEs in alleviating acute and chronic liver injury and tried to decipher the underlying mechanism.Methods and results Mice were divided into 5 groups(n ps:contro=8)for acute(groups:control,0.3%CCl_(4),bifendate(BD),100 and 200 mg/kg MFAEs,7 d)and chronic(groul,10%CCl_(4),BD,100 and 200 mg/kg MFAEs,4 weeks)liver injury study.Each mouse was injected intraperitoneally with 10μL/g corn oil containing CCl_(4)expect the control group.HepG2 cells were used in vitro study.Eighteen communal components were identified by UPLC-LTQOrbitrap-MS.We utilized a mouse model for acute and chronic liver injury using CCl_(4)and MFAEs administration effectively blocked fibrosis and significantly inhibited inflammation in the liver.MFAEs activated the nuclear factor erythroid derived 2 like 2/heme oxygenase 1(Nrf2/HO-1)pathway and promoted the synthesis of the antioxidants glutathione(GSH),superoxidedismutase(SOD)and glutathione peroxidase(GSH-Px)that resulted in reduced levels of CCl_(4)-induced oxidative stress molecules including reactive oxygen species.These extracts administered to mice also inhibited ferroptosis in the liver by regulating the expression of Acyl-CoA synthetase long chain family member 4(ACSL4),solute carrier family 7 member 11(SLC7A11)and glutathione peroxidase 4(GPX4),thus reducing the occurrence of liver fibrosis.Both in vivo and in vitro tests indicated that the mechanism of MFAEs protection against liver fibrosis was linked to activation of Nrf2 signaling.These effects were blocked in vitro by the addition of a specific Nrf2 inhibitor.Conclusion MFAEs inhibited oxidative stress,ferroptosis and inflammation of the liver by activating Nrf2 signal pathway and provided a significant protective effect against CCl_(4)-induced liver fibrosis. 展开更多
关键词 Acute and chronic liver injury Ferroptosis Mori fructus aqueous extracts NRF2 Oxidative stress
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Dietary oregano aqueous extract improves growth performance and intestinal health of broilers through modulating gut microbial compositions 被引量:1
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作者 Fan Zhang Jiantao Yang +5 位作者 Qinyi Zhan Hao Shi Yanhe Li Dinggang Li Yingge Li Xiaojun Yang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第5期2079-2092,共14页
Background Intestinal health plays a pivotal role in broiler chicken growth.Oregano aqueous extract(OAE)effec-tively exerts anti-inflammatory and antibacterial effects.However,the protective effects of OAE on intestin... Background Intestinal health plays a pivotal role in broiler chicken growth.Oregano aqueous extract(OAE)effec-tively exerts anti-inflammatory and antibacterial effects.However,the protective effects of OAE on intestinal health in broilers and the underlying mechanism remain unclear.This study aimed to investigate the potential effects of OAE on growth performance,the gut microbiota and intestinal health.A total of 8401-d-old male and female broilers(Arbor Acres)were randomly allocated into 6 groups as follows:basal diet(Con),Con+antibiotics(Anti,colistin sulfate 7 g/kg,roxarsone 35 g/kg),Con+400,500,600 and 700 mg/kg OAE(OAE400,OAE500,OAE600 and OAE700).Subse-quently,fermentation in vitro together with oral administration trials were carried out to further assess the function of OAE on intestinal health of broilers.Results Dietary 700 mg/kg OAE supplementation resulted in an increase(P<0.05)in body weight and a decrease(P<0.05)in feed conversion ratio when compared with the control during d 22 to 42 of the trial.OAE addition resulted in lower(P<0.05)jejunal crypt depth and mRNA expression of IL-4 and IL-10 at d 42.In addition,dietary OAE addition increased the abundance of Firmicutes(P=0.087)and Lactobacillus(P<0.05)in the cecum,and increased(P<0.05)the content of acetic acid and butyric acid.In the in vitro fermentation test,OAE significantly increased(P<0.05)the abundance of Lactobacillus,decreased(P<0.05)the abundance of unspecified_Enterobacteriaceae,and increased the content of acetic acid(P<0.05).In the oral administration trial,higher(P<0.05)IL-4 expression was found in broilers when oral inoculation with oregano fermentation microorganisms at d 42.And SIgA content in the ileum was significantly increased(P=0.073)when giving OAE fermentation supernatant.Conclusions Dietary OAE addition could maintain intestinal health and improve growth performance through enhancing intestinal mucosal immunity and barrier function mediated by gut microbiota changes. 展开更多
关键词 BROILERS Gut microbiota Intestinal health Oregano aqueous extract
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COSMO-RS prediction and experimental verification of 1,5-pentanediamine extraction from aqueous solution by ionic liquids 被引量:10
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作者 Chenhao Jiang Hongye Cheng +5 位作者 Zexian Qin Ruizhuan Wang Lifang Chen Chen Yang Zhiwen Qi Xiucai Liu 《Green Energy & Environment》 SCIE CSCD 2021年第3期422-431,共10页
1,5-Pentanediamine(PDA)produced by biological fermentation becomes popular,but the separation of PDA from the broth is a typical difficult problem.In this work,the performance of 200 ionic liquids(ILs),formed by combi... 1,5-Pentanediamine(PDA)produced by biological fermentation becomes popular,but the separation of PDA from the broth is a typical difficult problem.In this work,the performance of 200 ionic liquids(ILs),formed by combining 25 cations and 8 anions,in the extraction of PDA from aqueous solution were evaluated using COSMO-RS model.The extraction mechanism was investigated with the help ofσ-profile and interaction energy analyses.Both the cation and anion have impacts on the extraction efficiency,where cation mainly influences the interaction of IL with PDA and anion affects the hydrophobicity of IL.The IL composed of long alkyl-chain in cation and the anion of[PF6]-or[TF2 N]-,which has theσ-profile more likely distributed in the nonpolar region but less distributed in the polar region,is favorable for extraction.The experimental liquid-liquid equilibrium demonstrated the effects of cation and anion on extraction performance,which validated the reliability of COSMO-RS model in IL screening for PDA extraction.The IL[IM-1,8][PF6]could serve as a promising extractant for the downstream separation process of the biological production of PDA. 展开更多
关键词 1 5-Pentanediamine Ionic liquids aqueous solution extraction COSMO-RS model
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Measurement and Correlation of Equilibrium Data for Aqueous Two-phase System Ethanol+Water+ K_2HPO_4 被引量:4
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作者 LINJin-qing TANPing-hua JINChun-ying LIMing-chun 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2004年第2期195-199,共5页
The isothermal solubility data of aqueous two-phase system ethanol+water+K 2HPO 4 were determined with the turbidity titration method at 303.2 K. The binodal curves were described by using the Mistry equation very w... The isothermal solubility data of aqueous two-phase system ethanol+water+K 2HPO 4 were determined with the turbidity titration method at 303.2 K. The binodal curves were described by using the Mistry equation very well. An experimental procedure for measuring the liquid-liquid equilibrium data of the aqueous two-phase system was proposed, in which the concentrations of the coexisting phases were determined with the corresponding densities of the solution. The tie lines were satisfactorily described by using the Othmer-Tobias and Bancroft equations. 展开更多
关键词 aqueous two-phase system SOLUBILITY Turbidity titration LLE ETHANOL K 2HPO 4
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Aqueous Two-Phase Interfacial Assembly of COF Membranes for Water Desalination 被引量:5
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作者 Hongjian Wang Jiashuai Zhao +6 位作者 Yang Li Yu Cao Ziting Zhu Meidi Wang Runnan Zhang Fusheng Pan Zhongyi Jiang 《Nano-Micro Letters》 SCIE EI CAS CSCD 2022年第12期491-504,共14页
Aqueous two-phase system features with ultralow interfacial tension and thick interfacial region,affording unique confined space for membrane assembly.Here,for the first time,an aqueous two-phase interfacial assembly ... Aqueous two-phase system features with ultralow interfacial tension and thick interfacial region,affording unique confined space for membrane assembly.Here,for the first time,an aqueous two-phase interfacial assembly method is proposed to fabricate covalent organic framework(COF)membranes.The aqueous solution containing polyethylene glycol and dextran undergoes segregated phase separation into two water-rich phases.By respectively distributing aldehyde and amine monomers into two aqueous phases,a series of COF membranes are fabricated at water-water interface.The resultant membranes exhibit high NaCl rejection of 93.0-93.6% and water permeance reaching 1.7-3.7 L m^(−2) h^(−1) bar^(−1),superior to most water desalination membranes.Interestingly,the interfacial tension is found to have pronounced effect on membrane structures.The appropriate interfacial tension range(0.1-1.0 mN m^(−1))leads to the tight and intact COF membranes.Furthermore,the method is extended to the fabrication of other COF and metal-organic polymer membranes.This work is the first exploitation of fabricating membranes in all-aqueous system,confering a green and generic method for advanced membrane manufacturing. 展开更多
关键词 Covalent organic framework membranes aqueous two-phase Interfacial polymerization Molecular separation Water desalination
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Efficient Separation of Butyric Acid by an Aqueous Two-phase System with Calcium Chloride 被引量:4
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作者 吴丹 陈灏 +3 位作者 江凌 蔡谨 徐志南 岑沛霖 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2010年第4期533-537,共5页
One of the bottlenecks for bioproduction of butyric acid as bulk chemical is the difficulty in separating butyric acid from the fermentation broth,compared with the petroleum-based chemical synthesis method.In the pre... One of the bottlenecks for bioproduction of butyric acid as bulk chemical is the difficulty in separating butyric acid from the fermentation broth,compared with the petroleum-based chemical synthesis method.In the present work,a novel separation methodology was developed based on an aqueous two-phase system with inor-ganic salts.Calcium chloride was screened out for effective separation of butyric acid from butyric acid-water-salt systems.Within appropriate concentration range of butyric acid and salt,butyric acid was enriched in the upper phase and most of calcium ions remained in the lower phase.This"salting out"effect is very efficient to separate butyric acid from the simulated butyrate fermentation broth,which consists of butyric acid and acetic acid with concentration ratio of 4︰1,so that the final ratio of butyric acid/acetic acid in the upper phase is improved to 9.87. The aqueous two-phase system was used to separate butyric acid from the actual fermentation broth with satisfac-tory result. 展开更多
关键词 butyric acid SEPARATION aqueous two-phase partition salting out effect calcium chloride
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Multiple Response Optimization of High Temperature, Low Time Aqueous Extraction Process of Phenolic Compounds from Grape Byproducts 被引量:4
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作者 Hiba N. Rajha Nicolas Louka +4 位作者 Nada El Darra Zeina Hobaika Nadia Boussetta Eugène Vorobiev Richard G. Maroun 《Food and Nutrition Sciences》 2014年第4期351-360,共10页
In this study phenolic compounds extraction from grape byproducts was conducted using pure water as a solvent. High temperatures and low time incubation periods were used in the aim of reducing the cost of the process... In this study phenolic compounds extraction from grape byproducts was conducted using pure water as a solvent. High temperatures and low time incubation periods were used in the aim of reducing the cost of the process and heightening the phenolic compounds yield. Response surface methodology (RSM) was realized to study the effect of time and temperature on crushed and uncrushed grape pomace. The phenolic content was evaluated considering the quantity (total phenolics (TPC), flavonoids (FC), total monomeric anthocyanins (TMA) and tannins (TC)), and quality (antiradical activity (AA) and antioxidant capacity (AC)) of the extracts. High temperature low time extraction design used in this study was compared to the extraction process at moderate temperatures with relatively long periods of time. This was proved to ameliorate the quantitative extraction of phenolic compounds from grape pomace without affecting their bioactivity. Moreover, multiple response optimization showed the optimal extraction parameters to be 81?C and 140 minutes for the unmilled pomace samples, and 88?C and 5 minutes for the milled. TPC, FC, TMA, TC, AA and AC are almost the same for both optimums. Thus the possibility of replacing the milling process by the extraction time prolongation (for the unmilled pomace) of 135 minutes seems to be very plausible. HPLC analysis showed different quantity and diversity of extracted phenolics for the optimums. However this difference did not significantly affect the overall activity, showing that PC in the different extracts act in complete synergy all together leading to important biological properties. The obtained results using the extraction strategy adopted in this work could lead to several industrial applications. 展开更多
关键词 PHENOLIC Compounds GRAPE BYPRODUCTS OPTIMIZATION aqueous extraction RESPONSE Surface Methodology
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