Manganese(Mn)contamination limits the production and quality of crops,and affects human health by disrupting the food chain.Arabis paniculata is a pioneer species of Brassicaceae found in mining areas,and has the abil...Manganese(Mn)contamination limits the production and quality of crops,and affects human health by disrupting the food chain.Arabis paniculata is a pioneer species of Brassicaceae found in mining areas,and has the ability to accumulate heavy metals.However,little is known about the genetic mechanisms of Mn tolerance in A.paniculata.In this study,we found that Mn tolerance and ability to accumulate Mn were higher in A.paniculata than in Arabidopsis thaliana.The mechanisms underlying the response and recovery of A.paniculata to Mn toxicity were further investigated using transcriptome analysis.A total of 69,862,281 base pair clean reads were assembled into 61,627 high-quality unigenes,of which 41,591(67.5%)and 39,297(63.8%)were aligned in the Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO),respectively.In response to Mn toxicity,genes were expressed in twelve distinct patterns,which can be divided into four general categories:initial,stable,dose-dependent,and lineage.Genes that were differentially expressed during Mn response and recovery belong to several dominant KEGG pathways.An early response to Mn toxicity in A.paniculata includes the upregulation of genes involved in glutathione metabolism.ATP-binding cassette(ABC)transporter proteins were up-regulated during the entire response phase,and genes involved in glycerophospholipid metabolism were upregulated during the late phase of the Mn response.Genes in the phenylpropanoid pathway were differentially expressed in the repair process after Mn treatment.These findings reveal ideal material and genetic resources for phytoremediation in Mn-contaminated areas and highlight new knowledge and theoretical perspectives on the mechanisms of Mn tolerance.展开更多
Evaluation and grading of wool based on sex and age can help to improve economic value of wool. This study was conducted to investigate the wool production and fleece characteristics of Arabi sheep in Khuzestan provin...Evaluation and grading of wool based on sex and age can help to improve economic value of wool. This study was conducted to investigate the wool production and fleece characteristics of Arabi sheep in Khuzestan province The right mid-side wool samples were taken from 90 Arabi sheep (1 - 4 years;16 male and 74 female) by hand shearing. The staple length of shoulder, flank and rump were 13.18 ± 0.27, 13.31 ± 0.22 and 14.27 ± 0.25 cm, respectively. The greasy fleece weight, wool wax, vegetable matter, yield percentage, fiber diameter and its coefficient variation measures were 1.72 ± 0.06 Kg;2.77% ± 0.12%;0.54% ± 0.07%;73.36% ± 0.68%;30 ± 0.41 μm;30.23% ± 0.3% respectively. Correspondingly, the medulated fibers, true wool fiber and Kemp fibers were 21.6% ± 1.9%, 76.1% ± 2.04% and 2.5% ± 0.57% respectively. Age and sex had a significant influence on staple lengths. Fleece weight (P < 0.01) and wool wax were (P < 0.05) affected by sex. There was no significant interaction between age and sex groups for fiber characteristics, but it was important for staple between body location (P < 0.05). There were a low considerable kemp and CV of wool diameter percentage on Arabi sheep in comparison with the other Iranian native sheep. The wide variation in fleece weight (0.8 - 3.9 kg);mean staple lengths (8.8 - 19.2 cm);medulated and kemp (0 - 84.04 and 0% - 30.22%);fiber diameter (24.9 - 37.43 μm) suggest potential for improvement of economical traits.展开更多
Arabis stelleri var. japonica evidenced stronger osmotic stress tolerance than Arabidopsis thaliana. Using an A. thaliana microarray chip, we determined changes in the expression of approximately 2 800 genes between A...Arabis stelleri var. japonica evidenced stronger osmotic stress tolerance than Arabidopsis thaliana. Using an A. thaliana microarray chip, we determined changes in the expression of approximately 2 800 genes between A. stelleri plants treated with 0.2 M mannitol versus mock-treated plants. The most significant changes in the gene expression patterns were in genes defining cellular components or in genes associated with the endomembrane system, stimulus response, stress response, chemical stimulus response, and defense response. The expression patterns of three de novo proline biosynthesis enzymes were evaluated in A. stelleri var. japonica seedlings treated with 0.2 M mannitol, 0.2 M sorbitol, and 0.2 M NaCI. The expression of At-pyrroline-5-carboxylate synthetase was not affected by NaCI stress but was similarly induced by mannitol and sorbitol. The proline dehydrogenase gene, which is known to be repressed by dehydration stress and induced by free L-proline, was induced at an early stage by mannitol treatment, but the level of proline dehydrogenase was increased later by treatment with both mannitol and NaCI. The level of free L-proline accumulation increased progressively in response to treatments with mannitol, sorbitol, and NaCI. Mannitol induced L-proline accumulation more rapidly than NaCI or sorbitol. These findings demonstrate that the osmotic tolerance of the novel halophyte, Arabis stelleri, is associated with the accumulation of L-proline.展开更多
Araby is a short story by the famous Irish stream-of-consciousness writer James Joyce.Through a series of images,the novel expresses the theme of the story:the“mental paralysis”of Dubliners and the“spiritual Epiph...Araby is a short story by the famous Irish stream-of-consciousness writer James Joyce.Through a series of images,the novel expresses the theme of the story:the“mental paralysis”of Dubliners and the“spiritual Epiphany”of the little boy,which reflects the spiritual barren of Dubliners at that time.Through the analysis of the symbolic meaning of many images in the work,this paper reveals the social background and religious significance hidden behind the images.展开更多
基金This research was supported by Natural Science Foundation of Hunan Province,China(2020JJ4293)Scientific Research Project of the Hunan Education Department,China(18B215).
文摘Manganese(Mn)contamination limits the production and quality of crops,and affects human health by disrupting the food chain.Arabis paniculata is a pioneer species of Brassicaceae found in mining areas,and has the ability to accumulate heavy metals.However,little is known about the genetic mechanisms of Mn tolerance in A.paniculata.In this study,we found that Mn tolerance and ability to accumulate Mn were higher in A.paniculata than in Arabidopsis thaliana.The mechanisms underlying the response and recovery of A.paniculata to Mn toxicity were further investigated using transcriptome analysis.A total of 69,862,281 base pair clean reads were assembled into 61,627 high-quality unigenes,of which 41,591(67.5%)and 39,297(63.8%)were aligned in the Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO),respectively.In response to Mn toxicity,genes were expressed in twelve distinct patterns,which can be divided into four general categories:initial,stable,dose-dependent,and lineage.Genes that were differentially expressed during Mn response and recovery belong to several dominant KEGG pathways.An early response to Mn toxicity in A.paniculata includes the upregulation of genes involved in glutathione metabolism.ATP-binding cassette(ABC)transporter proteins were up-regulated during the entire response phase,and genes involved in glycerophospholipid metabolism were upregulated during the late phase of the Mn response.Genes in the phenylpropanoid pathway were differentially expressed in the repair process after Mn treatment.These findings reveal ideal material and genetic resources for phytoremediation in Mn-contaminated areas and highlight new knowledge and theoretical perspectives on the mechanisms of Mn tolerance.
文摘Evaluation and grading of wool based on sex and age can help to improve economic value of wool. This study was conducted to investigate the wool production and fleece characteristics of Arabi sheep in Khuzestan province The right mid-side wool samples were taken from 90 Arabi sheep (1 - 4 years;16 male and 74 female) by hand shearing. The staple length of shoulder, flank and rump were 13.18 ± 0.27, 13.31 ± 0.22 and 14.27 ± 0.25 cm, respectively. The greasy fleece weight, wool wax, vegetable matter, yield percentage, fiber diameter and its coefficient variation measures were 1.72 ± 0.06 Kg;2.77% ± 0.12%;0.54% ± 0.07%;73.36% ± 0.68%;30 ± 0.41 μm;30.23% ± 0.3% respectively. Correspondingly, the medulated fibers, true wool fiber and Kemp fibers were 21.6% ± 1.9%, 76.1% ± 2.04% and 2.5% ± 0.57% respectively. Age and sex had a significant influence on staple lengths. Fleece weight (P < 0.01) and wool wax were (P < 0.05) affected by sex. There was no significant interaction between age and sex groups for fiber characteristics, but it was important for staple between body location (P < 0.05). There were a low considerable kemp and CV of wool diameter percentage on Arabi sheep in comparison with the other Iranian native sheep. The wide variation in fleece weight (0.8 - 3.9 kg);mean staple lengths (8.8 - 19.2 cm);medulated and kemp (0 - 84.04 and 0% - 30.22%);fiber diameter (24.9 - 37.43 μm) suggest potential for improvement of economical traits.
基金supported by a grant from the Cooperative Research Projects for Bioenergy Crop Development RDA (RIMS20070201036026)
文摘Arabis stelleri var. japonica evidenced stronger osmotic stress tolerance than Arabidopsis thaliana. Using an A. thaliana microarray chip, we determined changes in the expression of approximately 2 800 genes between A. stelleri plants treated with 0.2 M mannitol versus mock-treated plants. The most significant changes in the gene expression patterns were in genes defining cellular components or in genes associated with the endomembrane system, stimulus response, stress response, chemical stimulus response, and defense response. The expression patterns of three de novo proline biosynthesis enzymes were evaluated in A. stelleri var. japonica seedlings treated with 0.2 M mannitol, 0.2 M sorbitol, and 0.2 M NaCI. The expression of At-pyrroline-5-carboxylate synthetase was not affected by NaCI stress but was similarly induced by mannitol and sorbitol. The proline dehydrogenase gene, which is known to be repressed by dehydration stress and induced by free L-proline, was induced at an early stage by mannitol treatment, but the level of proline dehydrogenase was increased later by treatment with both mannitol and NaCI. The level of free L-proline accumulation increased progressively in response to treatments with mannitol, sorbitol, and NaCI. Mannitol induced L-proline accumulation more rapidly than NaCI or sorbitol. These findings demonstrate that the osmotic tolerance of the novel halophyte, Arabis stelleri, is associated with the accumulation of L-proline.
文摘Araby is a short story by the famous Irish stream-of-consciousness writer James Joyce.Through a series of images,the novel expresses the theme of the story:the“mental paralysis”of Dubliners and the“spiritual Epiphany”of the little boy,which reflects the spiritual barren of Dubliners at that time.Through the analysis of the symbolic meaning of many images in the work,this paper reveals the social background and religious significance hidden behind the images.
