Sulfuric acid-phenol and sulfuric acid-anthrone methods were used to detect polysaccharide content in shoots of Aralia elata( Miq.) Seem.,and the conversion factor to glucose was measured with refined polysaccharide...Sulfuric acid-phenol and sulfuric acid-anthrone methods were used to detect polysaccharide content in shoots of Aralia elata( Miq.) Seem.,and the conversion factor to glucose was measured with refined polysaccharides. Comprehensive evaluation was carried out by linear relationship,precision,reproducibility,stability and recovery rate. The results showed that the linear relationship between glucose concentration and absorbance was good when glucose concentration was0-40 μg/ml,and the average recovery rate was equal to or higher than 97. 00% with good reproducibility( RSD 〈 1. 60%,n = 5). It revealed that the two methods were accurate and reliable,and suitable for the determination of polysaccharide content in the shoots of A. elata. Polysaccharide content detected by sulfuric acid-phenol and sulfuric acid-anthrone methods was 19. 31% and 20. 40% respectively.展开更多
Aim Aralia elata (Miq.) Seem is a medicinal plant with a variety of biological activities. Several trier- pene saponins extracted from the leaves of Aralia elata (Miq.) Seem possess potent cytotoxic activity, but ...Aim Aralia elata (Miq.) Seem is a medicinal plant with a variety of biological activities. Several trier- pene saponins extracted from the leaves of Aralia elata (Miq.) Seem possess potent cytotoxic activity, but the mechanisms remain unclear. Methods In the present study, we examined the anti-tumor activity of total saponins of Aralia elata (Miq.) Seem leaves (TSAESL) using both in vitro and in vivo experiments, and investigated the underlying molecular mechanisms. Results Briefly, TSAESL significantly inhibited the proliferation of five human cancer cell lines and induced apoptosis of MCF-7 cells in a concentration-dependent manner. TSAESL also in- creased the expression level of active caspase-3 and cleaved PARP. In addition, TSAESL dramatically activated the phosphorylation of ERK1/2, p38 and JNK in a concentration-dependent manner. Treatment with the ERK1/2 in- hibitor U0126, p38 inhibitor SB203580, or JNK inhibitor SP600125 prior to TSAESL exposure markedly attenuated TSAESL-induced phosphorylation of ERK1/2 and p38. In mice bearing MCF-7 xenografl, TSAESL markedly inhib- ited tumor growth without significantly affecting spleen coefficient and hematological parameters. Conclusion These results strongly suggest that TSAESL has anti-tumor effect via activating the MAPK-mediated signaling path- ways.展开更多
[Objectives]This article aimed to systematically study the biopharmaceutical characteristics of root bark and adventitious buds(tender stems and young leaves)of Aralia elata Seem.and to distinguish its root bark from ...[Objectives]This article aimed to systematically study the biopharmaceutical characteristics of root bark and adventitious buds(tender stems and young leaves)of Aralia elata Seem.and to distinguish its root bark from that of Acanthopanax gracilistylus,in order to avoid confusion in clinical medication and guide root segment cutting technology of A.elata Seem.[Methods]Samples were obtained by field collection,and using trait and microstructure identification techniques,systematic biopharmaceutical identification of samples was carried out.[Results]The trait and microscopic characteristics of the root bark and adventitious buds(tender stems and young leaves)of A.elata Seem.were clarified.[Conclusions]The traits,cross-sections and powder of root bark,tender stems and young leaves of A.elata Seem.all have unique identification characteristics.The secondary phloem of A.elata Seem.is particularly developed,and it provides adventitious buds with sufficient nutrients for their meristem.展开更多
AIM: To investigate the role of O-GIcNAcylation of nuclear factor-kappa B (NF-KB) in retinal ganglion cell (RGC) death and analysedthe effect of Aralia elata (AE) on neurodegen- eration in diabetic mice. METH...AIM: To investigate the role of O-GIcNAcylation of nuclear factor-kappa B (NF-KB) in retinal ganglion cell (RGC) death and analysedthe effect of Aralia elata (AE) on neurodegen- eration in diabetic mice. METHODS: C57BL/6mice with streptozotocin-induced diabetes were fed daily with AE extract or control (CTL) diet at the onset of diabetes mellitus (DM). Two months af- tar injection of streptozotocin or saline, the degree of cell death and the expression of O-GIcNAc transferase (OGT), N-acetyl-b-D-glucosaminidase (OGA), O-GIcNAcylated pro- teins, and O-GIcNAcylation of NF-KB were examined. RESULTS: AE did not affect the metabolic status of diabetic mice. The decrease in the inner retinal thickness (P〈0.001 vs CTL, P〈0.01 vs DM) and increases in RGCs with terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (P〈0.001 vs CTL, P〈0.0001 vs DM), glial activation, and active caspase-3 (P〈0.0001 vs CTL, P〈0.0001 vs DM) were blocked in diabetic retinas of AE extract-fed mice. Expression levels of protein O-GIcNAcylation and OGT were increased in diabetic retinas (P〈0.0001 vs CTL), and the level of O-GIcNAcylation of the NF-KB p65 subunit was higher in diabetic retinas than in controls (P〈0.0001 vs CTL). AE extract downregulated O-GIcNAcylation of NF-KB and prevented neurodegeneration induced by hyperglycemia (P〈0.0001 vs DM). CONCLUSION: O-GIcNAcylation of NF-KB is concerned in neuronal degeneration and that AE prevents diabetes-in- duced RGC apoptosis via downregulation of NF-KB O-GI- cNAcylation. Hence, O-GIcNAcylation may be a new object for the treatment of DR, and AE may have therapeutic pos- sibility to prevent diabetes-induced neurodegeneration.展开更多
The Araliaceae family consists of numerous species of medical plants of significant value as non-wood forest products.To conserve and culture these plants in natural forest stands is an important undertaking which sho...The Araliaceae family consists of numerous species of medical plants of significant value as non-wood forest products.To conserve and culture these plants in natural forest stands is an important undertaking which should be implemented according to the relationship between forest structure and understory population.In this study,thirtyfive plots were established in natural A ralia elata stands.Taller individual and denser populations were found in the northern and in the eastern regions,respectively.Both population densities and individual leaf weight increased along longitude.In contrast,their relationship with elevation and DBH were negative.Along with the altitude gradient,both height and root-collar diameter increased but population density declined.Root-collar diameter and population density decreased with latitude and DBH,respectively.Overall,dominant trees unlikely enforced strong disturbance to the development of understory A.elata populations unless concerning some specific topographic factors.展开更多
植物多向耐药性(Pleiotropic drug resistance,PDR)蛋白属于三磷酸腺苷结合盒(ATP-binding cassette,ABC)转运蛋白ABCG亚族,在植物次生代谢产物转运特别是萜类物质跨膜转运中发挥重要作用。基于辽东楤木全基因组测序鉴定出21个PDR转运蛋...植物多向耐药性(Pleiotropic drug resistance,PDR)蛋白属于三磷酸腺苷结合盒(ATP-binding cassette,ABC)转运蛋白ABCG亚族,在植物次生代谢产物转运特别是萜类物质跨膜转运中发挥重要作用。基于辽东楤木全基因组测序鉴定出21个PDR转运蛋白,且主要为细胞膜蛋白,该基因在12对染色体上均有分布,其中5号染色体上分布较多。共线性分析结果表明,部分PDR基因在进化过程中发生基因串联复制事件,并可能存在基因多拷贝扩增现象。基因结构分析结果表明,17个基因含有PDR基因特有结构域,且启动子上含有多种激素和非生物胁迫响应相关顺式作用元件。组织特异性表达模式分析结果表明,PDR基因主要在辽东楤木叶片中高表达。为筛选与辽东楤木三萜皂苷转运相关的PDR转运蛋白,对基因编码区序列、蛋白结构、跨膜螺旋等进行分析,初步筛选出7个结构完整的PDR转运蛋白。进一步通过系统进化分析、基因表达与皂苷含量的关联分析筛选出2个可能与楤木皂苷转运相关的PDR基因(AE02G00218和AE12G01725)。两个基因均受外源激素MeJA、SA和机械损伤诱导上调表达。以上研究为萜类物质转运相关蛋白鉴定与功能研究提供新思路和基因资源。展开更多
基金Supported by Scientific Research Project of Education Department of Liaoning Province,China(L2017lkyfwdf-05)Public Welfare Fund Project of Department of Science and Technology of Liaoning Province,China(2016003003)
文摘Sulfuric acid-phenol and sulfuric acid-anthrone methods were used to detect polysaccharide content in shoots of Aralia elata( Miq.) Seem.,and the conversion factor to glucose was measured with refined polysaccharides. Comprehensive evaluation was carried out by linear relationship,precision,reproducibility,stability and recovery rate. The results showed that the linear relationship between glucose concentration and absorbance was good when glucose concentration was0-40 μg/ml,and the average recovery rate was equal to or higher than 97. 00% with good reproducibility( RSD 〈 1. 60%,n = 5). It revealed that the two methods were accurate and reliable,and suitable for the determination of polysaccharide content in the shoots of A. elata. Polysaccharide content detected by sulfuric acid-phenol and sulfuric acid-anthrone methods was 19. 31% and 20. 40% respectively.
文摘Aim Aralia elata (Miq.) Seem is a medicinal plant with a variety of biological activities. Several trier- pene saponins extracted from the leaves of Aralia elata (Miq.) Seem possess potent cytotoxic activity, but the mechanisms remain unclear. Methods In the present study, we examined the anti-tumor activity of total saponins of Aralia elata (Miq.) Seem leaves (TSAESL) using both in vitro and in vivo experiments, and investigated the underlying molecular mechanisms. Results Briefly, TSAESL significantly inhibited the proliferation of five human cancer cell lines and induced apoptosis of MCF-7 cells in a concentration-dependent manner. TSAESL also in- creased the expression level of active caspase-3 and cleaved PARP. In addition, TSAESL dramatically activated the phosphorylation of ERK1/2, p38 and JNK in a concentration-dependent manner. Treatment with the ERK1/2 in- hibitor U0126, p38 inhibitor SB203580, or JNK inhibitor SP600125 prior to TSAESL exposure markedly attenuated TSAESL-induced phosphorylation of ERK1/2 and p38. In mice bearing MCF-7 xenografl, TSAESL markedly inhib- ited tumor growth without significantly affecting spleen coefficient and hematological parameters. Conclusion These results strongly suggest that TSAESL has anti-tumor effect via activating the MAPK-mediated signaling path- ways.
基金Scientific Research Project of Education Department of Liaoning(L2014502)Project of Liaoning Provincial Key Laboratory of Biomedical and Chemical EngineeringTraditional Chinese Medicine Related Scientific Research Project of Dalian City(18Z1002).
文摘[Objectives]This article aimed to systematically study the biopharmaceutical characteristics of root bark and adventitious buds(tender stems and young leaves)of Aralia elata Seem.and to distinguish its root bark from that of Acanthopanax gracilistylus,in order to avoid confusion in clinical medication and guide root segment cutting technology of A.elata Seem.[Methods]Samples were obtained by field collection,and using trait and microstructure identification techniques,systematic biopharmaceutical identification of samples was carried out.[Results]The trait and microscopic characteristics of the root bark and adventitious buds(tender stems and young leaves)of A.elata Seem.were clarified.[Conclusions]The traits,cross-sections and powder of root bark,tender stems and young leaves of A.elata Seem.all have unique identification characteristics.The secondary phloem of A.elata Seem.is particularly developed,and it provides adventitious buds with sufficient nutrients for their meristem.
基金Supported by the Basic Science Research Program Through the National Research Foundation(NRF)of Korea Funded by the Ministry of Science,ICT,and Future Planning 2014049413,NRF-2015R1A5A2008833 and NRF-2015R1C1A1A02037702
文摘AIM: To investigate the role of O-GIcNAcylation of nuclear factor-kappa B (NF-KB) in retinal ganglion cell (RGC) death and analysedthe effect of Aralia elata (AE) on neurodegen- eration in diabetic mice. METHODS: C57BL/6mice with streptozotocin-induced diabetes were fed daily with AE extract or control (CTL) diet at the onset of diabetes mellitus (DM). Two months af- tar injection of streptozotocin or saline, the degree of cell death and the expression of O-GIcNAc transferase (OGT), N-acetyl-b-D-glucosaminidase (OGA), O-GIcNAcylated pro- teins, and O-GIcNAcylation of NF-KB were examined. RESULTS: AE did not affect the metabolic status of diabetic mice. The decrease in the inner retinal thickness (P〈0.001 vs CTL, P〈0.01 vs DM) and increases in RGCs with terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (P〈0.001 vs CTL, P〈0.0001 vs DM), glial activation, and active caspase-3 (P〈0.0001 vs CTL, P〈0.0001 vs DM) were blocked in diabetic retinas of AE extract-fed mice. Expression levels of protein O-GIcNAcylation and OGT were increased in diabetic retinas (P〈0.0001 vs CTL), and the level of O-GIcNAcylation of the NF-KB p65 subunit was higher in diabetic retinas than in controls (P〈0.0001 vs CTL). AE extract downregulated O-GIcNAcylation of NF-KB and prevented neurodegeneration induced by hyperglycemia (P〈0.0001 vs DM). CONCLUSION: O-GIcNAcylation of NF-KB is concerned in neuronal degeneration and that AE prevents diabetes-in- duced RGC apoptosis via downregulation of NF-KB O-GI- cNAcylation. Hence, O-GIcNAcylation may be a new object for the treatment of DR, and AE may have therapeutic pos- sibility to prevent diabetes-induced neurodegeneration.
基金funded by the National Key Research and Development Program of China(Grant Number 2016YFC0500300)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant Number XDA23070503)+5 种基金the National Natural Science Foundation of China(Grant Numbers 419711224186101731600496)the Regional Key Project in S&T Services Network Program of the Chinese Academy of Sciences(Grant Numbers KFJ-STSQYZD-044KFJ-STS-ZDTP-048)the Funding for Jilin Environmental Science(Grant Number 2017-16)。
文摘The Araliaceae family consists of numerous species of medical plants of significant value as non-wood forest products.To conserve and culture these plants in natural forest stands is an important undertaking which should be implemented according to the relationship between forest structure and understory population.In this study,thirtyfive plots were established in natural A ralia elata stands.Taller individual and denser populations were found in the northern and in the eastern regions,respectively.Both population densities and individual leaf weight increased along longitude.In contrast,their relationship with elevation and DBH were negative.Along with the altitude gradient,both height and root-collar diameter increased but population density declined.Root-collar diameter and population density decreased with latitude and DBH,respectively.Overall,dominant trees unlikely enforced strong disturbance to the development of understory A.elata populations unless concerning some specific topographic factors.
文摘植物多向耐药性(Pleiotropic drug resistance,PDR)蛋白属于三磷酸腺苷结合盒(ATP-binding cassette,ABC)转运蛋白ABCG亚族,在植物次生代谢产物转运特别是萜类物质跨膜转运中发挥重要作用。基于辽东楤木全基因组测序鉴定出21个PDR转运蛋白,且主要为细胞膜蛋白,该基因在12对染色体上均有分布,其中5号染色体上分布较多。共线性分析结果表明,部分PDR基因在进化过程中发生基因串联复制事件,并可能存在基因多拷贝扩增现象。基因结构分析结果表明,17个基因含有PDR基因特有结构域,且启动子上含有多种激素和非生物胁迫响应相关顺式作用元件。组织特异性表达模式分析结果表明,PDR基因主要在辽东楤木叶片中高表达。为筛选与辽东楤木三萜皂苷转运相关的PDR转运蛋白,对基因编码区序列、蛋白结构、跨膜螺旋等进行分析,初步筛选出7个结构完整的PDR转运蛋白。进一步通过系统进化分析、基因表达与皂苷含量的关联分析筛选出2个可能与楤木皂苷转运相关的PDR基因(AE02G00218和AE12G01725)。两个基因均受外源激素MeJA、SA和机械损伤诱导上调表达。以上研究为萜类物质转运相关蛋白鉴定与功能研究提供新思路和基因资源。