Calcium influx into neurons triggers neuronal death during cerebral ischemia/reperfusion injury.Various calcium channels are involved in cerebral ischemia/reperfusion injury.Cav3.2 channel is a main subtype of T-type ...Calcium influx into neurons triggers neuronal death during cerebral ischemia/reperfusion injury.Various calcium channels are involved in cerebral ischemia/reperfusion injury.Cav3.2 channel is a main subtype of T-type calcium channels.T-type calcium channel blockers,such as pimozide and mibefradil,have been shown to prevent cerebral ischemia/reperfusion injury-induced brain injury.However,the role of Cav3.2 channels in cerebral ischemia/reperfusion injury remains unclear.Here,in vitro and in vivo models of cerebral ischemia/reperfusion injury were established using middle cerebral artery occlusion in mice and high glucose hypoxia/reoxygenation exposure in primary hippocampal neurons.The results showed that Cav3.2 expression was significantly upregulated in injured hippocampal tissue and primary hippocampal neurons.We further established a Cav3.2 gene-knockout mouse model of cerebral ischemia/reperfusion injury.Cav3.2 knockout markedly reduced infarct volume and brain water content,and alleviated neurological dysfunction after cerebral ischemia/reperfusion injury.Additionally,Cav3.2 knockout attenuated cerebral ischemia/reperfusion injury-induced oxidative stress,inflammatory response,and neuronal apoptosis.In the hippocampus of Cav3.2-knockout mice,calcineurin overexpression offset the beneficial effect of Cav3.2 knockout after cerebral ischemia/reperfusion injury.These findings suggest that the neuroprotective function of Cav3.2 knockout is mediated by calcineurin/nuclear factor of activated T cells 3 signaling.Findings from this study suggest that Cav3.2 could be a promising target for treatment of cerebral ischemia/reperfusion injury.展开更多
根据国际粮农组织(Food and Agriculture Organization of the United Nations,FAO)的国际植物检疫措施标准-检疫性有害生物风险分析(Pest Risk Analysis for Quarantine Pests Including Analysis of Environmental Risks andLiving Mo...根据国际粮农组织(Food and Agriculture Organization of the United Nations,FAO)的国际植物检疫措施标准-检疫性有害生物风险分析(Pest Risk Analysis for Quarantine Pests Including Analysis of Environmental Risks andLiving Modified Organisms)以及欧洲和地中海地区植物保护组织(European and Mediterranean Plant Protection Or-ganisation,EPPO)的有害生物风险评估方案,应用美国环境系统研究所(ESRI)开发的地理信息系统(GeographicInformation System,GIS)软件ArcView3.2对云南省129个县(市、区)的年平均气温、年平均降雨、日照、寄主分布和媒介昆虫等信息进行分析,对松材线虫(Bursaphelenchus xylophilus)进入可能性、定殖可能性和经济影响等方面进行了风险评估(Pest Risk Assessment,PRA)。划定松材线虫病发生低风险区2县、松材线虫病发生中度风险区20县(市、区)、松材线虫病发生较高风险区49县(市、区),其余县58县(市、区)为松材线虫病发生高度风险区。划定云南省北纬26°以南地区为重点防护区。并绘制寄主分布图、年平均气温图、松墨天牛分布图等风险评估地图。展开更多
基金supported by the Natural Science Foundation of Anhui Province of China,No.2208085Y32Scientific Research Plan Project of Anhui Province of China,No.2022AH020076the Chen Xiao-Ping Foundation for the Development of Science and Technology of Hubei Province,No.CXPJJH12000005-07-115(all to CT).
文摘Calcium influx into neurons triggers neuronal death during cerebral ischemia/reperfusion injury.Various calcium channels are involved in cerebral ischemia/reperfusion injury.Cav3.2 channel is a main subtype of T-type calcium channels.T-type calcium channel blockers,such as pimozide and mibefradil,have been shown to prevent cerebral ischemia/reperfusion injury-induced brain injury.However,the role of Cav3.2 channels in cerebral ischemia/reperfusion injury remains unclear.Here,in vitro and in vivo models of cerebral ischemia/reperfusion injury were established using middle cerebral artery occlusion in mice and high glucose hypoxia/reoxygenation exposure in primary hippocampal neurons.The results showed that Cav3.2 expression was significantly upregulated in injured hippocampal tissue and primary hippocampal neurons.We further established a Cav3.2 gene-knockout mouse model of cerebral ischemia/reperfusion injury.Cav3.2 knockout markedly reduced infarct volume and brain water content,and alleviated neurological dysfunction after cerebral ischemia/reperfusion injury.Additionally,Cav3.2 knockout attenuated cerebral ischemia/reperfusion injury-induced oxidative stress,inflammatory response,and neuronal apoptosis.In the hippocampus of Cav3.2-knockout mice,calcineurin overexpression offset the beneficial effect of Cav3.2 knockout after cerebral ischemia/reperfusion injury.These findings suggest that the neuroprotective function of Cav3.2 knockout is mediated by calcineurin/nuclear factor of activated T cells 3 signaling.Findings from this study suggest that Cav3.2 could be a promising target for treatment of cerebral ischemia/reperfusion injury.
文摘根据国际粮农组织(Food and Agriculture Organization of the United Nations,FAO)的国际植物检疫措施标准-检疫性有害生物风险分析(Pest Risk Analysis for Quarantine Pests Including Analysis of Environmental Risks andLiving Modified Organisms)以及欧洲和地中海地区植物保护组织(European and Mediterranean Plant Protection Or-ganisation,EPPO)的有害生物风险评估方案,应用美国环境系统研究所(ESRI)开发的地理信息系统(GeographicInformation System,GIS)软件ArcView3.2对云南省129个县(市、区)的年平均气温、年平均降雨、日照、寄主分布和媒介昆虫等信息进行分析,对松材线虫(Bursaphelenchus xylophilus)进入可能性、定殖可能性和经济影响等方面进行了风险评估(Pest Risk Assessment,PRA)。划定松材线虫病发生低风险区2县、松材线虫病发生中度风险区20县(市、区)、松材线虫病发生较高风险区49县(市、区),其余县58县(市、区)为松材线虫病发生高度风险区。划定云南省北纬26°以南地区为重点防护区。并绘制寄主分布图、年平均气温图、松墨天牛分布图等风险评估地图。
