HS-SPME-GC-MS Analysis on Volatile Components in Different Cultivars and Different Parts of Artemisia argyi

[Objectives] To analyze and compare the difference in volatile components of different cultivars and different parts of Artemisia argyi by using headspace solid phase microextraction( HS-SPME) and provide scientific basis for comprehensive development and rational utilization of Artemisia argyi. [Methods]Firstly,HS-SPME was used to extract the volatile components from the leaves,seeds,and stems of seven-tip Artemisia argyi,five-tip Artemisia argyi,and golden Artemisia argyi. Their chemical composition was analyzed by gas chromatographymass spectrometry( GC-MS),and the relative mass fraction of each component was determined by area normalization method. [Results] For the same variety of Artemisia argyi,the content of volatile components in leaves was higher than in seeds and stems except camphor,and the content of volatile components in seven-tip Artemisia argyi leaves was higher than in five-tip Artemisia argyi and golden Artemisia argyi leaves.HS-SPME-GC-MS analysis showed that eucalyptol,camphor,thujone,α-pinene,1-caryophyllene and caryophyllene oxide with higher content were detected in different parts of the different cultivars of Artemisia argyi. [Conclusions]Seven-tip Artemisia argyi leaves were the best medicinal parts of the three cultivars,followed by seven-tip Artemisia argyi seeds.

Analysis of Volatile Compounds and Identification of Characteristic Aroma Components of <i>Toona sinensis</i>(A. Juss.) Roem. Using GC-MS and GC-O

In this study, volatile compounds present in Toona sinensis (A. Juss.) Roem (TS) were investigated and their characteristic aromatic components were identified using Headspace Solid-phase Microextraction (HS-SPME) followed by Gas Chromatography-Mass Spectrometry (GC-MS) and Gas Chromatography-Olfactometry (GC-O). The optimum conditions for extracting the volatiles from TS were achieved with the experimental parameters including the use of a 65 μm polydimethylsiloxane/divinyl benzene (PDMS/DVB) fibre, an extraction temperature of 40℃ and an extraction time of 30 min. Under these conditions, 56 volatile compounds were separated and 53 were identified by GC-MS. Among them, 21 sulfide compounds (42.146%) and 27 terpenes(55.984%) were found to be the major components. The sample was analyzed by GC-O and 26 elutes were sniffed and their sensory descriptions evaluated by an odor panelists. Analysis of the data indicated, two compounds cis and trans isomers of 2-Mercapto-3,4-dimethyl-2,3-dihydrothiophene were major contributors to the characteristic aroma of TS.

GC-MS Analysis of Volatile Components Extracted from Fruit of Artemisia argyi

[Objectives] To analyze the volatile components extracted from fruit of Artemisia argyi.[Methods] The volatile components from fruit of Artemisia argyi were extracted by steam distillation( SD) and headspaces solid-phase microextraction( HS-SPME). The components were separated and analyzed by gas chromatography-mass spectrometry( GC-MS) with the area normalization method to determine the relative percentage content of each component.[Results]33 compounds were isolated and 29 compounds were identified by SD accounting for about93. 02% of the total volatile components; and 35 compounds were isolated and 30 compounds were identified by HS-SPME accounting for about99. 01% of the total volatile components. 11 kinds of the volatile components were identified by the two methods together and the content of β-thujone was highest.[Conclusions] There were some differences between SD and HS-SPME,and the two methods were applied to the extraction of different types of volatile components,and combination of the two methods to analyze the volatile components in fruit of Artemisia argyi could help us to get more comprehensive information.

SPME-GC-MS联用分析大腹皮中挥发性成分

目的:分析大腹皮中挥发性成分的化学组成。方法:采用顶空固相微萃取法(HS-SPME)萃取大腹皮中挥发性成分,并用气相色谱-质谱(GC-MS)联用技术对大腹皮的挥发性成分进行分析。结果:从大腹皮中共分离出60个色谱峰,鉴定出其中36种化合物,占总量的87.48%。其中主要成分为萘(54.93%)、二丁基羟基甲苯(7.21%)、3,4-二甲氧基甲苯(4.74%)等。结论:首次采用SPME-GC-MS联用技术分析大腹皮挥发性成分,为大腹皮的开发利用提供了科学依据。