The new generation of biomaterials focuses on the design of biomimetic polymeric materials that are capable of eliciting specific cellular responses and directing new tissue formation. Since Arg-Gly-Asp （RGD） sequen...The new generation of biomaterials focuses on the design of biomimetic polymeric materials that are capable of eliciting specific cellular responses and directing new tissue formation. Since Arg-Gly-Asp （RGD） sequences have been found to promote cell adhesion in 1984, numerous polymers have been functionalized with RGD peptides for tissue engineering applications. This review gave the advance in RGD modified biomimetic polymeric materials,focusing on the mechanism of RGD, the surface and bulk modification of polymer with RGD peptides and the evaluation in vitro and in vivo of the modified biomimetic materials.展开更多
采用碳端到氮端依次连接策略,以天冬氨酸、甘氨酸、精氨酸为起始原料,以HBTU为缩合剂,Asp(OBzl)-OAllyl顺次分别与Boc-甘氨酸和Cbz-Arg(Cbz)2偶联生成碳端和氮端保护的RGD。进一步在N-甲基吗啉存在下以Pd(PPh3)4催化脱去烯丙基,得到目...采用碳端到氮端依次连接策略,以天冬氨酸、甘氨酸、精氨酸为起始原料,以HBTU为缩合剂,Asp(OBzl)-OAllyl顺次分别与Boc-甘氨酸和Cbz-Arg(Cbz)2偶联生成碳端和氮端保护的RGD。进一步在N-甲基吗啉存在下以Pd(PPh3)4催化脱去烯丙基,得到目标产物。中间产物和目标产物的分离、提纯采用重结晶和柱层析法,应用1 H NMR,13C NMR和MS等对其结构进行了表征。展开更多
The cell adhesive motif RGD tripeptide was synthesized by using a novel chemical method. First, Gly-Asp（GD） was synthesized in two steps including the chloroacetylation of free L-aspartic acid and the ammonolysis of...The cell adhesive motif RGD tripeptide was synthesized by using a novel chemical method. First, Gly-Asp（GD） was synthesized in two steps including the chloroacetylation of free L-aspartic acid and the ammonolysis of the chloroacetylated L-aspartic acid. The yield of chloroacetylated L-aspartic acid was 83.0%. For the ammonolysis of chloroacetylated L-aspartic acid, the yield of the ammonolyzed product was 92. 3%. Second, the coupling between Arg and Gly-Asp was carried out by using the NCA method. The maximum yield of RGD was about 50% at 0℃ and pH = 9. 5. The prepared RGD tripeptide was confirmed by using amino acid component analysis and mass spectrographic analysis.展开更多
自组装的纳米纤维肽作为一种新型材料，被广泛应用于细胞的三维培养以及组织缺损的修复。本研究在传统自组装多肽RADA16—1（RAD16）的基础上，为成骨细胞体外培养构建了一种支架材料，即把细胞黏附基序arginine—glycine—aspartic（RG...自组装的纳米纤维肽作为一种新型材料，被广泛应用于细胞的三维培养以及组织缺损的修复。本研究在传统自组装多肽RADA16—1（RAD16）的基础上，为成骨细胞体外培养构建了一种支架材料，即把细胞黏附基序arginine—glycine—aspartic（RGD）连接到RAD16上，再与纯RAD16按比例混合，构成本实验的研究材料Hybrid。用原子力显微镜（atomic force microscope，AFM）观察材料的微观结构。通过相差显微镜、MTT、组织化学染色等工具或方法观察成骨细胞（MC3T3-E1）在材料中的生长、增殖和分化情况。结果显示，Hybrid可以形成良好的纳米纤维结构。与RAD16相比，这种改进的纳米材料可以促进细胞的增殖，且碱性磷酸酶（alkaline phosphatase，ALP）的表达呈强阳性，有明显的钙结节形成。实验表明该材料在骨组织工程方面有潜在的应用价值。展开更多
基金supported by the National Natural Science Foundation of China under grant No.30300084the Key Scientific and Technological Foundation of the State Ministry of Education in China.
文摘The new generation of biomaterials focuses on the design of biomimetic polymeric materials that are capable of eliciting specific cellular responses and directing new tissue formation. Since Arg-Gly-Asp （RGD） sequences have been found to promote cell adhesion in 1984, numerous polymers have been functionalized with RGD peptides for tissue engineering applications. This review gave the advance in RGD modified biomimetic polymeric materials,focusing on the mechanism of RGD, the surface and bulk modification of polymer with RGD peptides and the evaluation in vitro and in vivo of the modified biomimetic materials.
文摘采用碳端到氮端依次连接策略,以天冬氨酸、甘氨酸、精氨酸为起始原料,以HBTU为缩合剂,Asp(OBzl)-OAllyl顺次分别与Boc-甘氨酸和Cbz-Arg(Cbz)2偶联生成碳端和氮端保护的RGD。进一步在N-甲基吗啉存在下以Pd(PPh3)4催化脱去烯丙基,得到目标产物。中间产物和目标产物的分离、提纯采用重结晶和柱层析法,应用1 H NMR,13C NMR和MS等对其结构进行了表征。
基金Supported by the Special Research Grant from the State Administration of Traditional Chinese Medicine of China( No.2004ZDZX003).
文摘The cell adhesive motif RGD tripeptide was synthesized by using a novel chemical method. First, Gly-Asp（GD） was synthesized in two steps including the chloroacetylation of free L-aspartic acid and the ammonolysis of the chloroacetylated L-aspartic acid. The yield of chloroacetylated L-aspartic acid was 83.0%. For the ammonolysis of chloroacetylated L-aspartic acid, the yield of the ammonolyzed product was 92. 3%. Second, the coupling between Arg and Gly-Asp was carried out by using the NCA method. The maximum yield of RGD was about 50% at 0℃ and pH = 9. 5. The prepared RGD tripeptide was confirmed by using amino acid component analysis and mass spectrographic analysis.
文摘自组装的纳米纤维肽作为一种新型材料，被广泛应用于细胞的三维培养以及组织缺损的修复。本研究在传统自组装多肽RADA16—1（RAD16）的基础上，为成骨细胞体外培养构建了一种支架材料，即把细胞黏附基序arginine—glycine—aspartic（RGD）连接到RAD16上，再与纯RAD16按比例混合，构成本实验的研究材料Hybrid。用原子力显微镜（atomic force microscope，AFM）观察材料的微观结构。通过相差显微镜、MTT、组织化学染色等工具或方法观察成骨细胞（MC3T3-E1）在材料中的生长、增殖和分化情况。结果显示，Hybrid可以形成良好的纳米纤维结构。与RAD16相比，这种改进的纳米材料可以促进细胞的增殖，且碱性磷酸酶（alkaline phosphatase，ALP）的表达呈强阳性，有明显的钙结节形成。实验表明该材料在骨组织工程方面有潜在的应用价值。
