[Objectives]To establish a method for caffeic acid content determination and thin-layer chromatography identification of the Zhuang medicine Argyreia acuta Lour.[Methods]Silica gel GF254(thin-layer plate),toluene-form...[Objectives]To establish a method for caffeic acid content determination and thin-layer chromatography identification of the Zhuang medicine Argyreia acuta Lour.[Methods]Silica gel GF254(thin-layer plate),toluene-formic acid-ethyl acetate(V∶V∶V=5∶1.2∶3)(developing agent)and 365 nm ultraviolet light were used for thin-layer chromatography identification.Under the chromatographic conditions of column of Thermo SCIENTIFIC Hypersil C18(5μm,4.60 mm×250 mm),mobile phase of methanol-0.1%phosphoric acid(23∶77),detection wavelength of 320 nm,column temperature of 30℃,sample size of 10μL and flow rate of 1.0 mL/min,the content of caffeic acid in A.acuta Lour was determined.[Results]Caffeic acid can be detected in thin layer chromatography,with strong specificity and clear spots.When the sample size of caffeic acid is within the range of 0.0151-0.4530μg(R^(2)=0.9999),the content of caffeic acid showed a relatively good linear relationship with the peak area.The average recovery rate of A.acuta Lour was 99.94%(RSD=2.68%),97.56%(RSD=1.57%)and 99.79%(RSD=2.05%),respectively.[Conclusions]This method can effectively identify A.acuta Lour,and can accurately determine the content of caffeic acid in A.acuta Lour.It has characteristics of high accuracy,high precision,good color rendering stability,good reproducibility and fast analysis speed.展开更多
[Objectives]To explore the anti-inflammatory effect and mechanisms of different polar parts of Argyreia acuta Lour.[Methods]The effect of different polar parts of A.acuta Lour.on the viability of mouse RAW264.7 cells ...[Objectives]To explore the anti-inflammatory effect and mechanisms of different polar parts of Argyreia acuta Lour.[Methods]The effect of different polar parts of A.acuta Lour.on the viability of mouse RAW264.7 cells was detected by the CCK-8 method.Lipopolysaccharide(LPS)was used to stimulate RAW264.7 cells to establish an in vitro inflammation model,and the NO,TNF-αand IL-6 contents were determined by Griess method and ELISA assays,respectively.[Results]Within the range of 12.5-200μg/mL,all polar parts of A.acuta Lour.showed a proliferation effect on RAW264.7 cells,without cytotoxicity.The total extract,petroleum ether extract,ethyl acetate extract,and n-butanol extract all could inhibit the secretion of NO;and the petroleum ether extract and ethyl acetate extract could inhibit the secretion of TNF-αand IL-6.[Conclusions]The petroleum ether extract and ethyl acetate extract of A.acuta Lour.have significant anti-inflammatory activity,and the mechanism of action may be related to inhibiting the release of TNF-αand IL-6.展开更多
In the present study, three compounds were isolated from Argyreia acuta, among them, compounds 1 and 2 were new and Compounds 1 and 3 were isomers. They were separated by several types of columns, such as normal phase...In the present study, three compounds were isolated from Argyreia acuta, among them, compounds 1 and 2 were new and Compounds 1 and 3 were isomers. They were separated by several types of columns, such as normal phase, RP, size exclusion and preparative HPLC, and their structures were elucidated by several spectroscopic methods, such as 1D-and 2D-NMR and HR-TOF-MS.展开更多
基金National Characteristic TCM Technology Inheritance Talent Fostering Program(No.20184828005)Project for Enhancing Basic Scientific Research Ability of Young and Middle-aged Teachers in Colleges and Universities of Guangxi(No.2019KY0341)+1 种基金Natural Science Foundation of Guangxi(No.2020GXNSFAA259059)Prescription Development Project of the First Affiliated Hospital of Guangxi University of Chinese Medicine(No.2017ZJ001).
文摘[Objectives]To establish a method for caffeic acid content determination and thin-layer chromatography identification of the Zhuang medicine Argyreia acuta Lour.[Methods]Silica gel GF254(thin-layer plate),toluene-formic acid-ethyl acetate(V∶V∶V=5∶1.2∶3)(developing agent)and 365 nm ultraviolet light were used for thin-layer chromatography identification.Under the chromatographic conditions of column of Thermo SCIENTIFIC Hypersil C18(5μm,4.60 mm×250 mm),mobile phase of methanol-0.1%phosphoric acid(23∶77),detection wavelength of 320 nm,column temperature of 30℃,sample size of 10μL and flow rate of 1.0 mL/min,the content of caffeic acid in A.acuta Lour was determined.[Results]Caffeic acid can be detected in thin layer chromatography,with strong specificity and clear spots.When the sample size of caffeic acid is within the range of 0.0151-0.4530μg(R^(2)=0.9999),the content of caffeic acid showed a relatively good linear relationship with the peak area.The average recovery rate of A.acuta Lour was 99.94%(RSD=2.68%),97.56%(RSD=1.57%)and 99.79%(RSD=2.05%),respectively.[Conclusions]This method can effectively identify A.acuta Lour,and can accurately determine the content of caffeic acid in A.acuta Lour.It has characteristics of high accuracy,high precision,good color rendering stability,good reproducibility and fast analysis speed.
文摘[Objectives]To explore the anti-inflammatory effect and mechanisms of different polar parts of Argyreia acuta Lour.[Methods]The effect of different polar parts of A.acuta Lour.on the viability of mouse RAW264.7 cells was detected by the CCK-8 method.Lipopolysaccharide(LPS)was used to stimulate RAW264.7 cells to establish an in vitro inflammation model,and the NO,TNF-αand IL-6 contents were determined by Griess method and ELISA assays,respectively.[Results]Within the range of 12.5-200μg/mL,all polar parts of A.acuta Lour.showed a proliferation effect on RAW264.7 cells,without cytotoxicity.The total extract,petroleum ether extract,ethyl acetate extract,and n-butanol extract all could inhibit the secretion of NO;and the petroleum ether extract and ethyl acetate extract could inhibit the secretion of TNF-αand IL-6.[Conclusions]The petroleum ether extract and ethyl acetate extract of A.acuta Lour.have significant anti-inflammatory activity,and the mechanism of action may be related to inhibiting the release of TNF-αand IL-6.
基金supported by National Natural Science Fundation of China(Nos.81202885,81373931,81573877,and 81403074)the Natural Science Foundation of Guangdong Province of China(No.2015A030313585)
文摘In the present study, three compounds were isolated from Argyreia acuta, among them, compounds 1 and 2 were new and Compounds 1 and 3 were isomers. They were separated by several types of columns, such as normal phase, RP, size exclusion and preparative HPLC, and their structures were elucidated by several spectroscopic methods, such as 1D-and 2D-NMR and HR-TOF-MS.