期刊文献+
共找到7,298篇文章
< 1 2 250 >
每页显示 20 50 100
miR-340-5p靶向调控ARID1A对甲状腺癌活性、迁移和侵袭的影响
1
作者 段飞 陈羿 +1 位作者 查官金 段训凰 《中国老年学杂志》 CAS 北大核心 2024年第6期1480-1485,共6页
目的 探讨miR-340-5p靶向调控富含AT的相互作用结构域(ARID)1A对甲状腺癌活性、迁移和侵袭的影响。方法 通过实时荧光定-聚合酶链反应(RT-qPCR)和Western印迹检测miR-340-5p和ARID1A在甲状腺癌细胞中表达水平。通过噻唑蓝(MTT)和Transw... 目的 探讨miR-340-5p靶向调控富含AT的相互作用结构域(ARID)1A对甲状腺癌活性、迁移和侵袭的影响。方法 通过实时荧光定-聚合酶链反应(RT-qPCR)和Western印迹检测miR-340-5p和ARID1A在甲状腺癌细胞中表达水平。通过噻唑蓝(MTT)和Transwell实验检测转染miR-340-5p抑制物后癌细胞活性、迁移和侵袭变化。双荧光素酶报告基因检测miR-340-5p和ARID1A的靶向结合关系。最后通过同时敲低miR-340-5p和ARID1A的回复实验验证miR-340-5p调控ARID1A影响癌症发展。结果 miR-340-5p在甲状腺癌细胞中明显高表达,ARID1A表达明显较低(P<0.05)。通过抑制BCPAP细胞中miR-340-5p表达明显减少了癌细胞活性、迁移和侵袭水平。miR-340-5p和ARID1A具有直接靶向结合关系。敲降ARID1A能够明显逆转miR-340-5p抑制物对BCPAP细胞活性、迁移和侵袭的抑制作用。结论 miR-340-5p是甲状腺癌中的促癌基因,ARID1A为miR-340-5p的靶标下游,miR-340-5p通过靶向调控ARID1A促进甲状腺癌细胞活性、迁移和侵袭。 展开更多
关键词 甲状腺癌 miR-340-5p 富含AT的相互作用结构域(arid)1A 细胞活性 转移
下载PDF
结直肠癌组织中ARID1A基因突变及DEPDC1表达与临床病理特征及预后的相关性
2
作者 王准证 陈聪 施稳稳 《海南医学》 CAS 2024年第18期2590-2594,共5页
目的探讨结直肠癌中ARID1A基因突变及DEPDC1表达与患者临床病理特征及预后的相关性。方法回顾性分析2017年5月至2019年5月漯河市中心医院收治的152例结直肠癌患者的临床资料,所有患者均采用测序分析法和BCA法分析癌组织样本中ARID1A基... 目的探讨结直肠癌中ARID1A基因突变及DEPDC1表达与患者临床病理特征及预后的相关性。方法回顾性分析2017年5月至2019年5月漯河市中心医院收治的152例结直肠癌患者的临床资料,所有患者均采用测序分析法和BCA法分析癌组织样本中ARID1A基因的突变情况和DEPDC1的蛋白表达水平,依据ARID1A基因的突变情况和DEPDC1的蛋白表达水平分为ARID1A基因突变组、ARID1A基因未突变组和DEPDC1高表达组、DEPDC1低表达组,并评估ARID1A基因突变、DEPDC1表达与肿瘤的淋巴结转移、临床分期、大小和病理分化等临床病理特征的关系。使用Kaplan-Meier生存曲线分析ARID1A基因突变及DEPDC1高表达对患者5年生存率的影响。结果ARID1A基因突变组患者的低分化占比、Ⅲ~Ⅳ期占比、脉管侵犯占比均为63.64%(21/33)、54.55%(18/33)和60.61(20/33),明显高于ARID1A基因未突变组的29.41%(35/119)、24.37%(29/119)和31.93%(38/119),差异均有统计学意义(P<0.05);DEPDC1高表达组患者的Ⅲ~Ⅳ期占比、淋巴转移占比分别为46.51%(20/43)、53.49%(23/43),明显高于DEPDC1低表达组的24.77%(27/109)、24.77%(27/109),差异均有统计学意义(P<0.05);Kaplan-Meier生存分析结果显示,ARID1A基因未突变和DEPDC1低表达的患者5年生存率分别为70.59%和84.40%,明显高于ARID1A基因突变患者的39.39%和DEPDC1高表达患者的48.84%,差异均有统计学意义(P<0.05)。结论ARID1A基因突变及DEPDC1高表达可能在结直肠癌的发展和预后中发挥作用,为结直肠癌的分子分类和个体化治疗提供潜在的靶点。 展开更多
关键词 arid1A DEPDC1 结直肠癌 临床病理特征 预后
下载PDF
Estimating the Drought-Induced Yield Loss for Winter Wheat in a Semi-Arid Region of the Southern United States Using a Drought Index
3
作者 Prem Woli Qingwu Xue +2 位作者 Gerald R. Smith Charles R. Long Francis M. Rouquette Jr. 《Agricultural Sciences》 2024年第8期812-829,共18页
The economy of most rural locations in the semi-arid region of Llano Estacado in the southern United States is predominantly based on agriculture, primarily beef and wheat (Triticum aestivum L.) production. This regio... The economy of most rural locations in the semi-arid region of Llano Estacado in the southern United States is predominantly based on agriculture, primarily beef and wheat (Triticum aestivum L.) production. This region is prone to drought and is projected to experience a drier climate. Droughts that coincide with the critical phenological phases of a crop can be remarkably costly. Although drought cannot be prevented, its losses can be minimized through mitigation measures if it is predicted in advance. Predicting yield loss from an imminent drought is an important need of stakeholders. One way to fulfill this need is using an agricultural drought index, such as the Agricultural Reference Index for Drought (ARID). Being plant physiology-based, ARID can represent drought-yield relationships accurately. This study developed an ARID-based yield model for predicting the drought-induced yield loss for winter wheat in this region by accounting for its phenological phase-specific sensitivity to water stress. The reasonable values of the drought sensitivity coefficients of the yield model indicated that it could reflect the phenomenon of water stress decreasing the winter wheat yields in this region reasonably. The values of the various metrics used to evaluate the model, including Willmott Index (0.86), Nash-Sutcliffe Index (0.61), and percentage error (26), indicated that the yield model performed fairly well at predicting the drought-induced yield loss for winter wheat. The yield model may be useful for predicting the drought-induced yield loss for winter wheat in the study region and scheduling irrigation allocation based on phenological phase-specific drought sensitivity. 展开更多
关键词 arid DROUGHT Drought index Growth-stage Model Phenological-Phase Prediction SEMI-arid Wheat Yield
下载PDF
Spatiotemporal variability of rain-on-snow events in the arid region of Northwest China
4
作者 YANG Zhiwei CHEN Rensheng +3 位作者 LIU Zhangwen ZHAO Yanni LIU Yiwen WU Wentong 《Journal of Arid Land》 SCIE CSCD 2024年第4期483-499,共17页
Rain-on-snow(ROS)events involve rainfall on snow surfaces,and the occurrence of ROS events can exacerbate water scarcity and ecosystem vulnerability in the arid region of Northwest China(ARNC).In this study,using dail... Rain-on-snow(ROS)events involve rainfall on snow surfaces,and the occurrence of ROS events can exacerbate water scarcity and ecosystem vulnerability in the arid region of Northwest China(ARNC).In this study,using daily snow depth data and daily meteorological data from 68 meteorological stations provided by the China Meteorological Administration National Meteorological Information Centre,we investigated the spatiotemporal variability of ROS events in the ARNC from 1978 to 2015 and examined the factors affecting these events and possible changes of future ROS events in the ARNC.The results showed that ROS events in the ARNC mainly occurred from October to May of the following year and were largely distributed in the Qilian Mountains,Tianshan Mountains,Ili River Valley,Tacheng Prefecture,and Altay Prefecture,with the Ili River Valley,Tacheng City,and Altay Mountains exhibiting the most occurrences.Based on the intensity of ROS events,the areas with the highest risk of flooding resulting from ROS events in the ARNC were the Tianshan Mountains,Ili River Valley,Tacheng City,and Altay Mountains.The number and intensity of ROS events in the ARNC largely increased from 1978 to 2015,mainly influenced by air temperature and the number of rainfall days.However,due to the snowpack abundance in areas experiencing frequent ROS events in the ARNC,snowpack changes exerted slight impact on ROS events,which is a temporary phenomenon.Furthermore,elevation imposed lesser impact on ROS events in the ARNC than other factors.In the ARNC,the start time of rainfall and the end time of snowpack gradually advanced from the spring of the current year to the winter of the previous year,while the end time of rainfall and the start time of snowpack gradually delayed from autumn to winter.This may lead to more ROS events in winter in the future.These results could provide a sound basis for managing water resources and mitigating related disasters caused by ROS events in the ARNC. 展开更多
关键词 rain-on-snow events SNOWPACK SNOWMELT climate change Spearman's rank correlation arid region of Northwest China
下载PDF
Urban growth scenario projection using heuristic cellular automata in arid areas considering the drought impact
5
作者 TANG Xiaoyan FENG Yongjiu +7 位作者 LEI Zhenkun CHEN Shurui WANG Jiafeng WANG Rong TANG Panli WANG Mian JIN Yanmin TONG Xiaohua 《Journal of Arid Land》 SCIE CSCD 2024年第4期580-601,共22页
Arid areas with low precipitation and sparse vegetation typically yield compact urban pattern,and drought directly impacts urban site selection,growth processes,and future scenarios.Spatial simulation and projection b... Arid areas with low precipitation and sparse vegetation typically yield compact urban pattern,and drought directly impacts urban site selection,growth processes,and future scenarios.Spatial simulation and projection based on cellular automata(CA)models is important to achieve sustainable urban development in arid areas.We developed a new CA model using bat algorithm(BA)named bat algorithm-probability-of-occurrence-cellular automata(BA-POO-CA)model by considering drought constraint to accurately delineate urban growth patterns and project future scenarios of Urumqi City and its surrounding areas,located in Xinjiang Uygur Autonomous Region,China.We calibrated the BA-POO-CA model for the drought-prone study area with 2000 and 2010 data and validated the model with 2010 and 2020 data,and finally projected its urban scenarios in 2030.The results showed that BA-POO-CA model yielded overall accuracy of 97.70%and figure-of-merits(FOMs)of 35.50%in 2010,and 97.70%and 26.70%in 2020,respectively.The inclusion of drought intensity factor improved the performance of BA-POO-CA model in terms of FOMs,with increases of 5.50%in 2010 and 7.90%in 2020 than the model excluding drought intensity factor.This suggested that the urban growth of Urumqi City was affected by drought,and therefore taking drought intensity factor into account would contribute to simulation accuracy.The BA-POO-CA model including drought intensity factor was used to project two possible scenarios(i.e.,business-as-usual(BAU)scenario and ecological scenario)in 2030.In the BAU scenario,the urban growth dominated mainly in urban fringe areas,especially in the northern part of Toutunhe District,Xinshi District,and Midong District.Using exceptional and extreme drought areas as a spatial constraint,the urban growth was mainly concentrated in the"main urban areas-Changji-Hutubi"corridor urban pattern in the ecological scenario.The results of this research can help to adjust urban planning and development policies.Our model is readily applicable to simulating urban growth and future scenarios in global arid areas such as Northwest China and Africa. 展开更多
关键词 bat algorithm cellular automata(CA) probability-of-occurrence drought intensity algorithm-probability-of-occurrence-cellular automata(BA-POO-CA)model arid areas
下载PDF
Spatio-temporal variation of depth to groundwater level and its driving factors in arid and semi-arid regions of India
6
作者 Suchitra PANDEY Geetilaxmi MOHAPATRA Rahul ARORA 《Regional Sustainability》 2024年第2期103-122,共20页
Climate change and increasing anthropogenic activities,such as over-exploitation of groundwater,are exerting unavoidable stress on groundwater resources.This study investigated the spatio-temporal variation of depth t... Climate change and increasing anthropogenic activities,such as over-exploitation of groundwater,are exerting unavoidable stress on groundwater resources.This study investigated the spatio-temporal variation of depth to groundwater level(DGWL)and the impacts of climatic(precipitation,maximum temperature,and minimum temperature)and anthropogenic(gross district product(GDP),population,and net irrigated area(NIA))variables on DGWL during 1994-2020.The study considered DGWL in 113 observation wells and piezometers located in arid western plains(Barmer and Jodhpur districts)and semi-arid eastern plains(Jaipur,Ajmer,Dausa,and Tonk districts)of Rajasthan State,India.Statistical methods were employed to examine the annual and seasonal patterns of DGWL,and the generalized additive model(GAM)was used to determine the impacts of climatic and anthropogenic variables on DGWL.During 1994-2020,except for Barmer District,where the mean annual DGWL was almost constant(around 26.50 m),all other districts exhibited increase in DGWL,with Ajmer District experiencing the most increase.The results also revealed that 36 observation wells and piezometers showed a statistically significant annual increasing trend in DGWL and 34 observation wells and piezometers exhibited a statistically significant decreasing trend in DGWL.Similarly,32 observation wells and piezometers showed an statistically significant increasing trend and 37 observation wells and piezometers showed a statistically significant decreasing trend in winter;33 observation wells and piezometers indicated a statistically significant increasing trend and 34 had a statistically significant decreasing trend in post-monsoon;35 observation wells and piezometers exhibited a statistically significant increasing trend and 32 observation wells and piezometers showed a statistically significant decreasing trend in pre-monsoon;and 36 observation wells and piezometers reflected a statistically significant increasing trend and 30 observation wells and piezometers reflected a statistically significant decreasing trend in monsoon.Interestingly,most of the observation wells and piezometers with increasing trends of DGWL were located in Dausa and Jaipur districts.Furthermore,the GAM analysis revealed that climatic variables,such as precipitation,significantly affected DGWL in Barmer District,and DGWL in all other districts was influenced by anthropogenic variables,including GDP,NIA,and population.As a result,stringent regulations should be implemented to curb excessive groundwater extraction,manage agricultural water demand,initiate proactive aquifer recharge programs,and strengthen sustainable management in these water-scarce regions. 展开更多
关键词 Climate change Generalized additive model(GAM) Depth to groundwater level(DGWL) Climatic and anthropogenic variables arid and semi-arid regions
下载PDF
Innovative Technologies for Large-Scale Water Production in Arid Regions: Strategies for Sustainable Development
7
作者 Boris Menin 《Journal of Applied Mathematics and Physics》 2024年第7期2506-2558,共53页
Water scarcity in arid regions poses significant challenges to sustainable development and human well-being. This article explores both existing and innovative technologies and methods to produce large amounts of wate... Water scarcity in arid regions poses significant challenges to sustainable development and human well-being. This article explores both existing and innovative technologies and methods to produce large amounts of water to address these challenges effectively. Key approaches include atmospheric water generation, advanced desalination techniques, innovative water collection methods such as fog nets and dew harvesting, geothermal water extraction, and water recycling and reuse. Each method is evaluated for its feasibility with existing technology, potential time of implementation, required investments, and specific challenges. By leveraging these technologies and combining them into a multifaceted water management strategy, it is possible to enhance water security, support agricultural and industrial activities, and improve living conditions in arid regions. Collaborative efforts between governments, private sector entities, and research institutions are crucial to advancing these technologies and ensuring their sustainable implementation. The article provides a comprehensive overview of the current state of these technologies, their potential for large-scale application, and recommendations for future research and development. 展开更多
关键词 Atmospheric Water Generation Advanced Desalination Sustainable Development Geothermal Water Extraction Water Recycling arid Regions Water Security
下载PDF
基于ARID控制系统的固定式海洋风机纵摇控制 被引量:1
8
作者 张春巍 刘志虎 孙丽 《中国海洋平台》 2023年第4期9-17,共9页
针对固定式海洋风机在随机风浪载荷及其联合作用下产生的具有倒立摆运动特征的纵摇,研究采用主动转动惯量驱动系统(Active Rotary Inertia Driver,ARID)对海洋风机纵摇进行控制的问题。ARID控制系统通过伺服电机驱动转动惯性质量产生控... 针对固定式海洋风机在随机风浪载荷及其联合作用下产生的具有倒立摆运动特征的纵摇,研究采用主动转动惯量驱动系统(Active Rotary Inertia Driver,ARID)对海洋风机纵摇进行控制的问题。ARID控制系统通过伺服电机驱动转动惯性质量产生控制结构摆动的最优力矩,从而减小海洋风机的纵摇运动。基于拉格朗日原理建立海洋风机-ARID控制系统的理论分析模型;采用Simulink对海洋风机-ARID控制系统的有效性进行验证,并分析系统参数(控制算法参数、转动惯量比等)对控制效果的影响规律;设计海洋风机-ARID控制系统的振动台试验,设置多种载荷激励形式,验证控制系统的稳定性与广谱有效性。数值模拟和试验结果均验证了所建立的分析模型的正确性,表明ARID控制系统对风机的纵摇运动具有显著的控制作用,为ARID控制系统在具有倒立摆运动规律的工程结构中的应用奠定理论基础。 展开更多
关键词 固定式海洋风机 主动转动惯量驱动系统(arid) 纵摇 振动控制 倒立摆结构 振动台试验
下载PDF
玉米ARID转录因子家族鉴定及表达分析
9
作者 田玉红 吉虹菁 +5 位作者 罗堰木 全岩冰 黄敏 李曼菲 杜何为 刘睿 《南方农业学报》 CAS CSCD 北大核心 2023年第10期2821-2830,共10页
【目的】鉴定玉米ARID转录因子家族成员,并进行表达分析,为深入研究ARID转录因子生物学功能及玉米遗传育种提供理论参考。【方法】从拟南芥数据库TAIR获取7个ARID转录因子家族成员的氨基酸序列,与玉米基因组编码蛋白进行同源比对,鉴定... 【目的】鉴定玉米ARID转录因子家族成员,并进行表达分析,为深入研究ARID转录因子生物学功能及玉米遗传育种提供理论参考。【方法】从拟南芥数据库TAIR获取7个ARID转录因子家族成员的氨基酸序列,与玉米基因组编码蛋白进行同源比对,鉴定出玉米ARID转录因子家族成员,采用生物信息学方法对其理化性质、系统发育进化、启动子区顺式作用元件、生育期组织表达模式和蛋白互作网络等进行预测分析,并用实时荧光定量PCR检测其在激素处理及低温胁迫下的表达模式。【结果】从玉米中共鉴定出12个ARID转录因子家族成员(ZmARID1~ZmARID12),编码的氨基酸数量为448~1875个,均为亲水性蛋白,除ZmARID12外,其余均为不稳定蛋白;ZmARID2、ZmARID4、ZmARID9和ZmARID10蛋白定位于叶绿体和细胞核,ZmARID12定位于叶绿体和细胞质,ZmARID1定位于叶绿体,其余成员均定位于细胞核。玉米ARID家族基因启动子区域不仅含有多种光响应元件(G-box、Sp1、GATA-motif、Box 4、MRE、AE-box),还含有茉莉酸甲酯(MeJA)响应元件(CGTCA-motif)、脱落酸(ABA)响应元件(ABRE)、赤霉素(GA)响应元件(GARE-motif、TATC-box)、生长素(IAA)响应元件(TGA-element、AuxRR-core)和水杨酸(SA)响应元件(TCA-element)。ZmARID1基因在授粉后12 d(12 DAP)的胚乳中表达水平较高;除了ZmARID1和ZmARID3基因外,其余成员在2~4 mm和6~8 mm穗长的原基中均有较高表达;ZmARID3基因在成熟叶片和营养分生组织中表达水平较高;ZmARID5、ZmARID6和ZmARID11基因在成熟叶片中表达水平较高,说明部分家族成员表达具有组织特异性。实时荧光定量PCR检测结果显示,GA处理后,ZmARID12基因的相对表达量较对照显著升高(P<0.05,下同);ABA处理后,ARID家族基因相对表达量显著升高或下降,但IAA、JA、SA和低温胁迫处理后整体呈下降趋势。【结论】玉米ARID家族成员在全生育期具有明显的组织表达特异性,且在响应多种激素和低温胁迫中发挥重要作用。 展开更多
关键词 玉米 arid转录因子家族 生物信息学 鉴定 表达模式
下载PDF
Rainwater harvesting systems: An urban flood risk mitigation measure in arid areas 被引量:1
10
作者 Rouya Hdeib Marwan Aouad 《Water Science and Engineering》 EI CAS CSCD 2023年第3期219-225,共7页
Rainwater harvesting (RWH) systems have been developed to compensate for shortage in the water supply worldwide. Such systems are not very common in arid areas, particularly in the Gulf Region, due to the scarcity of ... Rainwater harvesting (RWH) systems have been developed to compensate for shortage in the water supply worldwide. Such systems are not very common in arid areas, particularly in the Gulf Region, due to the scarcity of rainfall and their reduced efficiency in covering water demand and reducing water consumption rates. In spite of this, RWH systems have the potential to reduce urban flood risks, particularly in densely populated areas. This study aimed to assess the potential use of RWH systems as urban flood mitigation measures in arid areas. Their utility in the retention of stormwater runoff and the reduction of water depth and extent were evaluated. The study was conducted in a residential area in Bahrain that experienced waterlogging after heavy rainfall events. The water demand patterns of housing units were analyzed, and the daily water balance for RWH tanks was evaluated. The effect of the implementation of RWH systems on the flood volume was evaluated with a two-dimensional hydrodynamic model. Flood simulations were conducted in several rainfall scenarios with different probabilities of occurrence. The results showed significant reductions in the flood depth and flood extent, but these effects were highly dependent on the rainfall intensity of the event. RWH systems are effective flood mitigation measures, particularly in urban arid regions short of proper stormwater control infrastructure, and they enhance the resilience of the built environment to urban floods. 展开更多
关键词 Rainwater harvesting Urban floods Flood map Hydrodynamic model Built environment arid areas
下载PDF
ARID5B基因SNP与ALL患儿MTX耐药性的相关性
11
作者 张丽芬 马钰 +2 位作者 李莲 刘文娥 张晓春 《中国实验血液学杂志》 CAS CSCD 北大核心 2023年第2期333-337,共5页
目的:探讨ARID5B基因单核苷酸多态性(SNP)与急性淋巴细胞白血病(ALL)患儿甲氨蝶呤(MTX)耐药性的相关性。方法:纳入2015年1月至2021年11月于宁夏医科大学总院治疗的144例ALL患儿,分为MTX耐药组和非MTX耐药组,每组72例,采用基质辅助激光... 目的:探讨ARID5B基因单核苷酸多态性(SNP)与急性淋巴细胞白血病(ALL)患儿甲氨蝶呤(MTX)耐药性的相关性。方法:纳入2015年1月至2021年11月于宁夏医科大学总院治疗的144例ALL患儿,分为MTX耐药组和非MTX耐药组,每组72例,采用基质辅助激光解吸电离飞行时间质谱技术测量所有患儿的ARID5B基因SNP,分析其与患儿MTX耐药性的相关性。结果:两组患儿rs7923074、rs10821936、rs6479778、rs2893881位点基因型及基因频率比较无统计学差异(P>0.05);MTX耐药组C/C基因型频率明显高于非MTX耐药组,而T/T基因型频率则相反(P<0.05);MTX耐药组C等位基因频率明显高于非MTX耐药组,而T等位基因频率则相反(P<0.05);多因素Logistic回归分析结果显示,ARID5B基因rs4948488 TT基因型、T等位基因频率是ALL患儿MTX耐药性的危险因素(P<0.05)。结论:ARID5B基因SNP与ALL患儿MTX耐药性具相关性。 展开更多
关键词 arid5B基因 单核苷酸多态性 急性淋巴细胞白血病 甲氨蝶呤 耐药性
下载PDF
LncRNA IQCH-AS1调节miR-494-3p/ARID1A轴抑制孕激素耐药的子宫内膜癌细胞增殖 被引量:1
12
作者 强贤 欧阳俊 +1 位作者 王海珍 葛素梅 《现代肿瘤医学》 CAS 北大核心 2023年第18期3335-3341,共7页
目的:探讨LncRNA IQCH-AS1调节miR-494-3p/ARID1A轴对孕激素耐药的子宫内膜癌(EC)细胞增殖、凋亡的影响。方法:体外培养Ishikawa细胞,构建EC孕激素耐药细胞株Ishikawa/醋酸甲羟孕酮(MPA),MTT法验证Ishikawa/MPA细胞的耐药性;qRT-PCR法检... 目的:探讨LncRNA IQCH-AS1调节miR-494-3p/ARID1A轴对孕激素耐药的子宫内膜癌(EC)细胞增殖、凋亡的影响。方法:体外培养Ishikawa细胞,构建EC孕激素耐药细胞株Ishikawa/醋酸甲羟孕酮(MPA),MTT法验证Ishikawa/MPA细胞的耐药性;qRT-PCR法检测Ishikawa细胞和Ishikawa/MPA细胞中孕激素受体(PR)、LncRNA IQCH-AS1、miR-494-3p和ARID1A mRNA表达。双荧光素酶报告基因实验、RNA pull-down实验验证Ishikawa/MPA细胞中LncRNA IQCH-AS1、miR-494-3p和ARID1A间靶向关系。Ishikawa/MPA细胞分为Ad-NC组、Ad-IQCH-AS1组、Ad-IQCH-AS1+miR-NC组和Ad-IQCH-AS1+miR-494-3p组,qRT-PCR法检测LncRNA IQCH-AS1、miR-494-3p、ARID1AmRNA、PR mRNA表达;MTT检测细胞增殖活力;流式细胞仪检测细胞凋亡率;Western blot法检测ARID1A、PR、CyclinD1、CDK4、cleaved-caspase-3、cleaved-PARP蛋白表达。裸鼠成瘤实验观察LncRNA IQCH-AS1对Ishikawa/MPA细胞裸鼠移植瘤生长和MPA耐药性的影响。结果:本研究构建的Ishikawa/MPA细胞耐药指数为4.33。Ishikawa/MPA细胞中PR mRNA、LncRNA IQCH-AS1和ARID1A mRNA低表达,而miR-494-3p高表达(P<0.05)。经验证,Ishikawa/MPA细胞中miR-494-3p与LncRNA IQCH-AS1、ARID1A均存在靶向关系。Ishikawa/MPA细胞中过表达LncRNA IQCH-AS1能够下调miR-494-3p表达,同时上调ARID1A、PR mRNA和蛋白表达,降低细胞存活率和CyclinD1、CDK4蛋白表达,提高细胞凋亡率和cleaved-caspase-3、cleaved-PARP蛋白表达(P<0.05);而过表达miR-494-3p可减弱过表达LncRNA IQCH-AS1对ARID1A、PR表达以及细胞增殖、凋亡的影响。此外,过表达LncRNA IQCH-AS1可抑制Ishikawa/MPA细胞的裸鼠移植瘤生长,并降低MPA耐药性。结论:LncRNA IQCH-AS1调节miR-494-3p/ARID1A轴抑制孕激素耐药的EC细胞增殖,并诱导其凋亡。 展开更多
关键词 LncRNA IQCH-AS1 miR-494-3p arid1A 孕激素 耐药性 子宫内膜癌
下载PDF
ARID1A下调促进甲状腺癌细胞增殖和凋亡的作用机制
13
作者 达布西力特 郑皓 +1 位作者 韩承新 肖晶晶 《医学研究杂志》 2023年第4期117-122,共6页
目的 ARID1A是SWI/SNF染色质复合体的关键成员,该基因已成为人类各种癌症的肿瘤抑制基因。分析了ARID1A在甲状腺癌中的表达情况和临床意义。方法 利用免疫组化技术对甲状腺癌患者组织与癌旁组织进行检测,并结合聚合酶链反应(polymerase ... 目的 ARID1A是SWI/SNF染色质复合体的关键成员,该基因已成为人类各种癌症的肿瘤抑制基因。分析了ARID1A在甲状腺癌中的表达情况和临床意义。方法 利用免疫组化技术对甲状腺癌患者组织与癌旁组织进行检测,并结合聚合酶链反应(polymerase chain reaction, PCR)技术了解ARID1A基因在甲状腺癌中的mRNA表达情况。此外,本研究还通过蛋白质印迹技术、MTT增殖试验、集落形成试验以及细胞凋亡检测技术探究ARID1A在甲状腺癌发生、发展中对肿瘤细胞增殖和凋亡影响的作用机制。结果 与正常腺上皮比较,甲状腺癌组织中ARID1A表达降低,并且与淋巴结转移、TNM分期和分化差具有显著的相关性,并且ARID1A在甲状腺癌细胞系中的表达低于正常腺上皮TEC细胞系。本研究还进一步探讨了ARID1A在甲状腺癌细胞系生物学行为中的作用,FTC133和BCPAP细胞系中转染小干扰RNA(siRNA)的ARID1A缺失促进增殖、集落形成能力,并抑制细胞凋亡。结论 ARID1A可能是甲状腺癌中的重要肿瘤抑制因子,该基因的下调能够促进甲状腺癌细胞增殖,抑制细胞凋亡。 展开更多
关键词 arid1A 甲状腺癌 细胞增殖 凋亡 生存预后 基础研究
下载PDF
Combination of artificial zeolite and microbial fertilizer to improve mining soils in an arid area of Inner Mongolia,China
14
作者 LI Wenye ZHANG Jianfeng +5 位作者 SONG Shuangshuang LIANG Yao SUN Baoping WU Yi MAO Xiao LIN Yachao 《Journal of Arid Land》 SCIE CSCD 2023年第9期1067-1083,共17页
Restoration of mining soils is important to the vegetation and environment.This study aimed to explore the variations in soil nutrient contents,microbial abundance,and biomass under different gradients of substrate am... Restoration of mining soils is important to the vegetation and environment.This study aimed to explore the variations in soil nutrient contents,microbial abundance,and biomass under different gradients of substrate amendments in mining soils to select effective measures.Soil samples were collected from the Bayan Obo mining region in Inner Mongolia Autonomous Region,China.Contents of soil organic matter(SOM),available nitrogen(AN),available phosphorus(AP),available potassium(AK),microbial biomass carbon/microbial biomass nitrogen(MBC/MBN)ratio,biomass,and bacteria,fungi,and actinomycetes abundance were assessed in Agropyron cristatum L.Gaertn.,Elymus dahuricus Turcz.,and Medicago sativa L.soils with artificial zeolite(AZ)and microbial fertilizer(MF)applied at T0(0 g/kg),T1(5 g/kg),T2(10 g/kg),and T3(20 g/kg).Redundancy analysis(RDA)and technique for order preference by similarity to ideal solution(TOPSIS)were used to identify the main factors controlling the variation of biomass.Results showed that chemical indices and microbial content of restored soils were far greater than those of control.The application of AZ significantly increases SOM,AN,and AP by 20.27%,23.61%,and 40.43%,respectively.AZ significantly increased bacteria,fungi,and actinomycetes abundance by 0.63,3.12,and 1.93 times of control,respectively.RDA indicated that AN,MBC/MBN ratio,and SOM were dominant predictors for biomass across samples with AZ application,explaining 87.6%of the biomass variance.SOM,MBC/MBN ratio,and AK were dominant predictors with MF application,explaining 82.9%of the biomass variance.TOPSIS indicated that T2 was the best dosage and the three plant species could all be used to repair mining soils.AZ and MF application at T2 concentration in the mining soils with M.sativa was found to be the most appropriate measure. 展开更多
关键词 AMENDMENT arid area mining soils RESTORATION soil nutrition
下载PDF
GIS-based flash flooding susceptibility analysis and water management in arid mountain ranges:Safaga Region,Red Sea Mountains,Egypt
15
作者 Ahmed E.EL-RAYES Mohamed O.ARNOUS Ahmed M.HELMY 《Journal of Mountain Science》 SCIE CSCD 2023年第12期3665-3686,共22页
The Safaga Region(SR)is part of the Red Sea mountain range in Egypt.Catastrophic flash flooding is now an inescapable event,wreaking havoc and causing massive loss of life and property.The majority of the floodwater,h... The Safaga Region(SR)is part of the Red Sea mountain range in Egypt.Catastrophic flash flooding is now an inescapable event,wreaking havoc and causing massive loss of life and property.The majority of the floodwater,however,has been wasted as runoff to the Red Sea,which,if used wisely,could meet a fraction of the water demands for a variety of applications in this area.The current work aims to use GIS techniques to integrate remote sensing data for evaluating,mitigating,and managing flash floods in SR.The data set comprised Tropical Rainfall Measuring Mission(TRMM)thematic rainfall data,1:50,000 scale topographical map sheets,geological maps,the ASTER Digital Elevation Model(ASTER GDEM),Landsat 7 Enhanced Thematic Mapper"(ETM7+),and Landsat 8 Operational Land Imager.The flash flood risk model of SR is developed using ArcGIS-10.3 geoprocessing tools integrating all the causal factors thematic maps.The final flood risk model for the SR suggests that 57%of the total basins in the SR are at high risk of flooding.Almost 38%of all basins are at moderate flood risk.The remaining 5%of basins are less prone to flooding.Flood-prone zones were identified,suitable dam-building sites were located,and extremely probable areas for water recharge were recognized.On the basis of reliable scientific data,structural and non-structural mitigation strategies that might reduce the damage susceptibility,alleviate the sensitivity of the flash flood,and best utilize its water supply were recommended. 展开更多
关键词 Flash floods GIS SUSCEPTIBILITY Water management arid mountains EGYPT
下载PDF
Evaluation of restoration success in arid rangelands of Iran based on the variation of ecosystem services
16
作者 Mohsen SHARAFATMANDRAD Azam KHOSRAVI MASHIZI 《Journal of Arid Land》 SCIE CSCD 2023年第11期1290-1314,共25页
The plantation of non-native species is one of the most expensive ecological restoration measures in arid and semi-arid areas,while its impacts on local communities are largely ignored.This study assessed the rate of ... The plantation of non-native species is one of the most expensive ecological restoration measures in arid and semi-arid areas,while its impacts on local communities are largely ignored.This study assessed the rate of change and the dynamic degree of the economic values of ecosystem services related to local conservation(water yield,stocking rate and aesthetic value)and preserving the future(carbon sequestration,soil protection,soil stability and habitat provision)to determine the restoration success of the plantation of non-native species Haloxylon ammodendron(C.A.Mey.)Bunge ex Fenzl(15-and 30-year-old)in parts of arid rangelands of Bardsir region,Kerman Province,Iran.We investigated the impacts of the two plantations on the seven ecosystem services and ecosystem structures(horizontal and vertical structures,vegetation composition and species diversity)based on field sampling and measurements at four sampling sites(i.e.,control,degraded,and 15-and 30-year-old plantation sites)in spring and summer of 2022.The restoration success of the plantation of non-native species was then examined by assessing the rate of change and the dynamic degree of the total economic value of all ecosystem services as well as the rate of change and the dynamic degree of the economic values of ecosystem services for the two groups(local conservation and preserving the future).Although the plantation of non-native species H.ammodendron enormously improved the vertical and horizontal structures of ecosystems,it failed to increase species diversity and richness fully.Further,despite the plantation of non-native species H.ammodendron had significantly increased the economic values of all ecosystem services,it was only quite successful in restoring carbon sequestration.Path analysis showed that plantation age had a significant impact on restoration success directly and indirectly(through changing ecosystem structures and services).The dynamic degree of the economic values of ecosystem services related to local conservation and preserving the future at the 15-and 30-year-old plantation sites indicated that the two plantations successfully restored the ecosystem services related to preserving the future.The presented method can help managers select the best restoration practices and predict their ecological-social success,especially for the plantation of high-risk non-native species in arid and semi-arid areas. 展开更多
关键词 Haloxylon ammodendron restoration success ecosystem services ecosystem structures arid ecosystems path analysis Iran
下载PDF
Indicator of climate variability:low treeline displacement in arid valleys of mountain areas,China
17
作者 YAN Wei-po WANG Qing +3 位作者 GUO Ya-lin HU Qi YANG Min AN Yi-da 《Journal of Mountain Science》 SCIE CSCD 2023年第11期3250-3265,共16页
As climate change intensifies,finding an ecological indicator to quickly and accurately reflect the impact on mountain ecosystems is necessary.The low treeline/timberline,highly sensitive to climate variability and ch... As climate change intensifies,finding an ecological indicator to quickly and accurately reflect the impact on mountain ecosystems is necessary.The low treeline/timberline,highly sensitive to climate variability and changes significantly within 5–10years,provides a new way to study the response to regional climate variability.This study explored the distribution and vertical displacement patterns of the low treeline in the Upper Minjiang River of China,using SPOT remote sensing images in 1999 and 2013and long-term positional observations.Using the Geodetector model,the study investigated the dominant climatic factors influencing the low treeline displacement.The results showed that the low treeline was located at 1700–3200 m elevation on sunny slopes(southeast,south,southwest,and west slopes)with slopes over 25°.From 1999 to 2013,the low treeline moved downward by 6 m from 2561±264m to 2555±265 m,along with a warm–humid climate tendency.The downward displacement was greater on slopes over 25°and shady slopes(-20 m and-10 m,respectively)than on slopes≤25°and sunny slopes.Additionally,the downward was greater in the warm and humid Zagunao River Basin(-15 m)compared to the arid valley center(-7 m)and the cold Heishui River Basin(-3 m).Meanwhile,the low treeline displacement correlated negatively with precipitation and relative humidity variations at the significance level of 0.05,with correlation coefficients of-0.572and-0.551,respectively.Variations in relative humidity and temperature significantly affected the spatial differentiation of low treeline displacement with influencing power of 0.246(p=0.036<0.05)and 0.183(p=0.032<0.05),respectively.Thus,the low treeline is a moisture-limited line,and its formation and variation are closely related to regional water–heat balance.The study clarifies the indicative value of the low treeline for climate variability in mountain areas and can provide references for ecological restoration in arid valleys. 展开更多
关键词 Low treeline Moisture-limited line Climate variability Remote sensing interpretation Geo-detector arid valley
下载PDF
Selecting proper sites for underground dam construction using Multi-Attribute Utility Theory in arid and semi-arid regions
18
作者 Mohammad Hassan SADEGHIRAVESH Hassan KHOSRAVI Azam ABOLHASANI 《Journal of Mountain Science》 SCIE CSCD 2023年第1期197-208,共12页
Although the construction of underground dams is one of the best methods to conserve water resources in arid and semi-arid regions,applying efficient methods for the selection of suitable sites for subsurface dam cons... Although the construction of underground dams is one of the best methods to conserve water resources in arid and semi-arid regions,applying efficient methods for the selection of suitable sites for subsurface dam construction remains a challenge.Due to the costly and time-consuming methods of site selection for underground dam construction,this study aimed to present a new method using geographic information systems techniques and decision-making processes.The exclusionary criteria including fault,slope,hypsometry,land use,soil,stream,geology,and chemical properties of groundwater were selected for site selection of dam construction and inappropriate regions were omitted by integration and scoring layers in ArcGIS based on the Boolean logic.Finally,appropriate sites were prioritized using the Multi-Attribute Utility Theory.According to the results of the utility coefficient,seven sites were selected as the region for underground dam construction based on all criteria and experts’opinions.The site of Nazarabad dam was the best location for underground dam construction with a utility coefficient of 0.7137 followed by sites of Akhavan with a utility coefficient of 0.4633 and Mirshamsi with a utility coefficient of 0.4083.This study proposed a new approach for the construction of the subsurface dam at the proper site and help managers and decision-makers achieve sustainable water resources with limited facilities and capital and avoid wasting national capital. 展开更多
关键词 PRIORITIZATION Geographic information system Multi-Attribute decision making arid region
下载PDF
Erratum to:ARID1A Inactivation Increases Expression of circ0008399 and Promotes Cisplatin Resistance in Bladder Cancer
19
作者 Yang-kai JIANG Yu-jun SHUAI +7 位作者 Hua-min DING Hui ZHANG Chao HUANG Liang WANG Jia-yin SUN Wen-jie WEI Xing-yuan XIAO Guo-song JIANG 《Current Medical Science》 SCIE CAS 2023年第6期1260-1260,共1页
The original version of this article was revised due to production error by the vendor.The author“Hua-min DING”is one of the co-authors,and the name should be labeled correctly as appears on PDF.The affiliation of“... The original version of this article was revised due to production error by the vendor.The author“Hua-min DING”is one of the co-authors,and the name should be labeled correctly as appears on PDF.The affiliation of“Yu-jun SHUAI”and“Chao HUANG”is“Department of Urology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China”,and both of them should be labeled as 1,as correctively appears on PDF. 展开更多
关键词 arid1A CANCER CISPLATIN
下载PDF
Reclamation during oasification is conducive to the accumulation of the soil organic carbon pool in arid land
20
作者 YANG Yuxin GONG Lu TANG Junhu 《Journal of Arid Land》 SCIE CSCD 2023年第3期344-358,共15页
Soil organic carbon(SOC)and its stable isotope composition reflect key information about the carbon cycle in ecosystems.Studies of carbon fractions in oasis continuous cotton-cropped fields can elucidate the SOC stabi... Soil organic carbon(SOC)and its stable isotope composition reflect key information about the carbon cycle in ecosystems.Studies of carbon fractions in oasis continuous cotton-cropped fields can elucidate the SOC stability mechanism under the action of the human-land relationship during the oasification of arid land,which is critical for understanding the carbon dynamics of terrestrial ecosystems in arid lands under global climate change.In this study,we investigated the Alar Reclamation Area on the northern edge of the Tarim Basin,Xinjiang Uygur Autonomous Region of China,in 2020.In original desert and oasis farmlands with different reclamation years,including 6,10,18,and 30 a,and different soil depths(0-20,20-40,40-60 cm),we analyzed the variations in SOC,very liable carbon(C_(VL)),liable carbon(C_(L)),less liable carbon(C_(LL)),and non-liable carbon(C_(NL))using the method of spatial series.The differences in the stable carbon isotope ratio(δ^(13)C)and beta(β)values reflecting the organic carbon decomposition rate were also determined during oasification.Through redundancy analysis,we derived and discussed the relationships among SOC,carbon fractions,δ^(13)C,and other soil physicochemical properties,such as the soil water content(SWC),bulk density(BD),pH,total salt(TS),total nitrogen(TN),available phosphorus(AP),and available potassium(AK).The results showed that there were significant differences in SOC and carbon fractions of oasis farmlands with different reclamation years,and the highest SOC was observed at the oasis farmland with 30-a reclamation year.C_(VL),C_(L),C_(LL),and C_(NL) showed significant changes among oasis farmlands with different reclamation years,and C_(VL) had the largest variation range(0.40-4.92 g/kg)and accounted for the largest proportion in the organic carbon pool.The proportion of C_(NL) in the organic carbon pool of the topsoil(0-20 cm)gradually increased.δ^(13)C varied from-25.61‰to-22.58‰,with the topsoil showing the most positive value at the oasis farmland with 10-a reclamation year;while theβvalue was the lowest at the oasis farmland with 6-a reclamation year and then increased significantly.Based on the redundancy analysis results,the soil physicochemical properties,such as TN,AP,AK,and pH,were significantly correlated with C_(L),and TN and AP were positively correlated with C_(VL).However,δ^(13)C was not significantly influenced by soil physicochemical properties.Our analysis advances the understanding of SOC dynamics during oasification,revealing the risk of soil carbon loss and its contribution to terrestrial carbon accumulation in arid lands,which could be useful for the sustainable development of regional carbon resources and ecological protection in arid ecosystem. 展开更多
关键词 OASIFICATION soil organic carbon carbon fractions labile carbon δ^(13)C arid land
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部