The genus Armeniaca Scop. is well known for its popular cultivated edible fruit trees such as Armeniaca vul- garis Lam. and ornamental flowers such as A. mume Sieb. Another species, A. cathayana Fu et al., one of six ...The genus Armeniaca Scop. is well known for its popular cultivated edible fruit trees such as Armeniaca vul- garis Lam. and ornamental flowers such as A. mume Sieb. Another species, A. cathayana Fu et al., one of six important dry fruits （kernel-using apricot）, is cultivated for its edible seeds in North China. In the present study, DNA from 70 individuals of A rmeniaca, including 38 of A. cathayana, 18 ofA. vulgaris, 12 ofA. sibirica, 1 ofA. dasycarpa and 1 of A. mume, was extracted and analyzed using microsatellites and capillary electrophoresis. For 20 polymorphic loci selected, 339 alleles and 140.7 effective alleles were detected. The number of alleles per locus ranged from 8 to 28, with an average of 16.95 alleles per locus. The observed heterozygosity （Ho） and the expected heterozygosity （He） ranged from 0.427 to 0.971 and from 0.737 to 0.912, respectively. The polymorphism information content varied from 0.708 to0.905, with an average of 0.827. Based on the genetic similarity among 70 individuals, a UPGMA was used to establish the phylogenetic relationships. The taxonomic positions among five species were clearly revealed, and A. cathayana was more closely related to A. vulgaris than to A. sibirica. The results will provide a scientific basis for research on the taxonomy, germplasm resources and breeding ofArmeniaca, especially for A. cathayana.展开更多
[ Objective] This study aimed to investigate the genetic variation between hybrids and their parents, and to find a rapid identification method. [ Method ] The progenies between Prunus armeniaca （ apricot ） Katy and...[ Objective] This study aimed to investigate the genetic variation between hybrids and their parents, and to find a rapid identification method. [ Method ] The progenies between Prunus armeniaca （ apricot ） Katy and Prunus salicina （plum） Moriteni were analyzed by means of RAPD and SSR molecular markers. [ Result] We found the differences in the electrophoresis bands between progenies and their parents, indicating that hybrid seedlings obtained in this study were real. [ Conclusion ] The combination of the above methods is effective and fast to identify the hybrids, and can shorten the breeding period.展开更多
Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of ...Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of efficacy of their compatibility.Methods:SPF Kunming male mice were fed with high sugar and high fat diet for 6 weeks and then intraperitoneally injected with STZ solution to establish a diabetes model.The successfully modeled mice were randomly divided into 6 groups according to their body weight:model group,Abrus Cantoniensis group,Armeniaca mume Sieb group,as well as Abrus Cantoniensis and Armeniaca mume Sieb group divided into 1:2,1:1 and 2:1 groups according to volume ratio with 10 mice in each group.Another 10 healthy mice were taken as blank group and intraperitoneally injected with sodium citrate buffer.The corresponding solution was given intragastric administration by body mass once a day for 20 days.After the last administration,fasting was performed for 12 hours.Serum was collected by the method of eyeball blood collection to determine the contents of fasting blood glucose,total cholesterol Triacylglycerol,triglyceride,low density lipoprotein and high-density lipoprotein.Results:Compared with Abrus Cantoniensis and Armeniaca mume Sieb groups,Glu,TG,TC and LDL-C of the three compatibility groups significantly decreased,with the most significant decrease in the compatibility group 1:2,while there was no significant difference in HDL-C among all groups.Conclusion:Abrus Cantoniensis and Armeniaca mume Sieb and their combination have synergistic effects on the improvement of blood glucose and lipid levels in mice with high glucose and high fat combined with STZ-induced diabetes mellitus model.Among all the experimental groups,the compatibility 1:2 group had the best effect.展开更多
[ Objective] The aim was to establish the optimal ISSR-PCR system for Armeniaca sibirica. [ Method] With Armeniaca sibirica as experimental materi- al, genomic DNA was extracted from tender leaves using CTAB method. O...[ Objective] The aim was to establish the optimal ISSR-PCR system for Armeniaca sibirica. [ Method] With Armeniaca sibirica as experimental materi- al, genomic DNA was extracted from tender leaves using CTAB method. Orthogonal experiment L9 （34） was conducted with four factors （Mg2＋ , dNTPs, primers, Taq DNA polymerase） in ISSR-PCR system ofArmeniaca sibirica at three levels. The results of orthogonal experimental design were analyzed by using SPSS statisti- cal software. [Result] Different levels of various factors all had significant effects on PCR results, specifically, the amount of Taq DNA polymerase had the most significant effect. The optimal ISSR-PCR reaction system of Armeniaca sibirica was established ： the total PCR reaction volume was 20 ~1, containing 1.0 U of Taq DNA polymerase, 1.0 mmol/L MgCI2,0.15 mmol/L dNTPs and 0.30 ~mol/L primers. Validation result shows that this reaction system is very stable and strongly operational. [ Conclusion] This study laid the foundation for genetic diversity analysis and germplasm resource investigation of Arnniaca sibirica.展开更多
[Objectives]To analyze the genetic similarity among varieties of Armeniaca vulgaris Shushanggan in Ili.[Methods]The genetic distance among 13 A.vulgaris Shushanggan varieties was compared at the DNA molecular level,an...[Objectives]To analyze the genetic similarity among varieties of Armeniaca vulgaris Shushanggan in Ili.[Methods]The genetic distance among 13 A.vulgaris Shushanggan varieties was compared at the DNA molecular level,and clustering analysis was performed on them.[Results]The 13 varieties of A.vulgaris Shushanggan were classified into four clusters.The first cluster included Shushang Ganxing 1,Shushang Ganxing 4,Gongliu Yexing and Zhenzhu Youxing,and the similarity coefficient between Shushang Ganxing 1 and Shushang Ganxing4 reached 0.808;the second cluster were Shushang Ganxing 2,Shushang Ganxing 3 and Liguangxing,and the similarity coefficient between Shushang Ganxing 2 and Shushang Ganxing 3 was 0.846[1];Ili Baixing,61 Tuan Guyexing,Chaxian Yexing and Huocheng Yexing were classified into the third cluster,and the similarity coefficient between Ili Baixing and 61 Tuan Guyexing was 0.692;and the fourth cluster included Shushang Ganxing W1 and Yixian Yexing,between which the similarity coefficient was 0.692.[Conclusions]This will lay a foundation for the research of variety identification and genetic structure of A.vulgaris Shushanggan.展开更多
基金financially supported by 948 Project(No.2011-4-37)Standardization Project(No.2013-LY-082) of The State Forestry Administration of China
文摘The genus Armeniaca Scop. is well known for its popular cultivated edible fruit trees such as Armeniaca vul- garis Lam. and ornamental flowers such as A. mume Sieb. Another species, A. cathayana Fu et al., one of six important dry fruits （kernel-using apricot）, is cultivated for its edible seeds in North China. In the present study, DNA from 70 individuals of A rmeniaca, including 38 of A. cathayana, 18 ofA. vulgaris, 12 ofA. sibirica, 1 ofA. dasycarpa and 1 of A. mume, was extracted and analyzed using microsatellites and capillary electrophoresis. For 20 polymorphic loci selected, 339 alleles and 140.7 effective alleles were detected. The number of alleles per locus ranged from 8 to 28, with an average of 16.95 alleles per locus. The observed heterozygosity （Ho） and the expected heterozygosity （He） ranged from 0.427 to 0.971 and from 0.737 to 0.912, respectively. The polymorphism information content varied from 0.708 to0.905, with an average of 0.827. Based on the genetic similarity among 70 individuals, a UPGMA was used to establish the phylogenetic relationships. The taxonomic positions among five species were clearly revealed, and A. cathayana was more closely related to A. vulgaris than to A. sibirica. The results will provide a scientific basis for research on the taxonomy, germplasm resources and breeding ofArmeniaca, especially for A. cathayana.
基金Supported by the Natural Science Foundation of Shandong Province(Q2008D01)Program for Science Research Development of Higher Education Institutions of Shandong Province(J09LC54)
文摘[ Objective] This study aimed to investigate the genetic variation between hybrids and their parents, and to find a rapid identification method. [ Method ] The progenies between Prunus armeniaca （ apricot ） Katy and Prunus salicina （plum） Moriteni were analyzed by means of RAPD and SSR molecular markers. [ Result] We found the differences in the electrophoresis bands between progenies and their parents, indicating that hybrid seedlings obtained in this study were real. [ Conclusion ] The combination of the above methods is effective and fast to identify the hybrids, and can shorten the breeding period.
基金This work was supported by National Natural Science Foundation of China(No.81360684)Guangxi Key Research and Development Plan Project(Gui Ke AB18221095)+3 种基金Teaching Teacher Training Project from Youjiang Medical University for Nationalities-National Teaching Teacher Training Project(You Hospital Zi[2018]No.98)High-level Talent Research Projects from Youjiang Medical University For Nationalities(No.01002018079)China National and Regional University Students Innovation and Entrepreneurship Training Scheme Funding(No.202010599022)China Regional University Students Innovation and Entrepreneurship Training Scheme Funding(No.202010599073).
文摘Objective:The study aims to explore the interventional effect of Abrus cantonensis and Armeniaca mume Sieb and their compatibility on blood glucose and blood lipid in diabetic mice and to explore the optimal ratio of efficacy of their compatibility.Methods:SPF Kunming male mice were fed with high sugar and high fat diet for 6 weeks and then intraperitoneally injected with STZ solution to establish a diabetes model.The successfully modeled mice were randomly divided into 6 groups according to their body weight:model group,Abrus Cantoniensis group,Armeniaca mume Sieb group,as well as Abrus Cantoniensis and Armeniaca mume Sieb group divided into 1:2,1:1 and 2:1 groups according to volume ratio with 10 mice in each group.Another 10 healthy mice were taken as blank group and intraperitoneally injected with sodium citrate buffer.The corresponding solution was given intragastric administration by body mass once a day for 20 days.After the last administration,fasting was performed for 12 hours.Serum was collected by the method of eyeball blood collection to determine the contents of fasting blood glucose,total cholesterol Triacylglycerol,triglyceride,low density lipoprotein and high-density lipoprotein.Results:Compared with Abrus Cantoniensis and Armeniaca mume Sieb groups,Glu,TG,TC and LDL-C of the three compatibility groups significantly decreased,with the most significant decrease in the compatibility group 1:2,while there was no significant difference in HDL-C among all groups.Conclusion:Abrus Cantoniensis and Armeniaca mume Sieb and their combination have synergistic effects on the improvement of blood glucose and lipid levels in mice with high glucose and high fat combined with STZ-induced diabetes mellitus model.Among all the experimental groups,the compatibility 1:2 group had the best effect.
基金Supported by Start-up Fund for High-level Scientific Research Personnel(11GK30)
文摘[ Objective] The aim was to establish the optimal ISSR-PCR system for Armeniaca sibirica. [ Method] With Armeniaca sibirica as experimental materi- al, genomic DNA was extracted from tender leaves using CTAB method. Orthogonal experiment L9 （34） was conducted with four factors （Mg2＋ , dNTPs, primers, Taq DNA polymerase） in ISSR-PCR system ofArmeniaca sibirica at three levels. The results of orthogonal experimental design were analyzed by using SPSS statisti- cal software. [Result] Different levels of various factors all had significant effects on PCR results, specifically, the amount of Taq DNA polymerase had the most significant effect. The optimal ISSR-PCR reaction system of Armeniaca sibirica was established ： the total PCR reaction volume was 20 ~1, containing 1.0 U of Taq DNA polymerase, 1.0 mmol/L MgCI2,0.15 mmol/L dNTPs and 0.30 ~mol/L primers. Validation result shows that this reaction system is very stable and strongly operational. [ Conclusion] This study laid the foundation for genetic diversity analysis and germplasm resource investigation of Arnniaca sibirica.
文摘[Objectives]To analyze the genetic similarity among varieties of Armeniaca vulgaris Shushanggan in Ili.[Methods]The genetic distance among 13 A.vulgaris Shushanggan varieties was compared at the DNA molecular level,and clustering analysis was performed on them.[Results]The 13 varieties of A.vulgaris Shushanggan were classified into four clusters.The first cluster included Shushang Ganxing 1,Shushang Ganxing 4,Gongliu Yexing and Zhenzhu Youxing,and the similarity coefficient between Shushang Ganxing 1 and Shushang Ganxing4 reached 0.808;the second cluster were Shushang Ganxing 2,Shushang Ganxing 3 and Liguangxing,and the similarity coefficient between Shushang Ganxing 2 and Shushang Ganxing 3 was 0.846[1];Ili Baixing,61 Tuan Guyexing,Chaxian Yexing and Huocheng Yexing were classified into the third cluster,and the similarity coefficient between Ili Baixing and 61 Tuan Guyexing was 0.692;and the fourth cluster included Shushang Ganxing W1 and Yixian Yexing,between which the similarity coefficient was 0.692.[Conclusions]This will lay a foundation for the research of variety identification and genetic structure of A.vulgaris Shushanggan.
