[ Objective] The aim was to establish the optimal ISSR-PCR system for Armeniaca sibirica. [ Method] With Armeniaca sibirica as experimental materi- al, genomic DNA was extracted from tender leaves using CTAB method. O...[ Objective] The aim was to establish the optimal ISSR-PCR system for Armeniaca sibirica. [ Method] With Armeniaca sibirica as experimental materi- al, genomic DNA was extracted from tender leaves using CTAB method. Orthogonal experiment L9 （34） was conducted with four factors （Mg2＋ , dNTPs, primers, Taq DNA polymerase） in ISSR-PCR system ofArmeniaca sibirica at three levels. The results of orthogonal experimental design were analyzed by using SPSS statisti- cal software. [Result] Different levels of various factors all had significant effects on PCR results, specifically, the amount of Taq DNA polymerase had the most significant effect. The optimal ISSR-PCR reaction system of Armeniaca sibirica was established ： the total PCR reaction volume was 20 ~1, containing 1.0 U of Taq DNA polymerase, 1.0 mmol/L MgCI2,0.15 mmol/L dNTPs and 0.30 ~mol/L primers. Validation result shows that this reaction system is very stable and strongly operational. [ Conclusion] This study laid the foundation for genetic diversity analysis and germplasm resource investigation of Arnniaca sibirica.展开更多
对陕西吴旗县引种栽培的龙王帽、一窝蜂和优一3个大扁杏品种的光响应曲线、净光合速率(P n)、蒸腾速率(T r)、气孔导度(G s)和水分利用效率(WUE)等生理特性指标进行了测定和比较分析。结果表明:龙王帽、一窝蜂和优一的光饱和点分别为600...对陕西吴旗县引种栽培的龙王帽、一窝蜂和优一3个大扁杏品种的光响应曲线、净光合速率(P n)、蒸腾速率(T r)、气孔导度(G s)和水分利用效率(WUE)等生理特性指标进行了测定和比较分析。结果表明:龙王帽、一窝蜂和优一的光饱和点分别为600,600和400μm o l/(m2.s);日均P n排序为一窝蜂[4.07μm o l/(m2.s)]>龙王帽[3.75μm o l/(m2.s)]>优一[3.17μm o l/(m2.s)],日均T r排序为一窝蜂[1.74 mm o l/(m2.s)]>龙王帽[1.51mm o l/(m2.s)]>优一[1.29 mm o l/(m2.s)],日均WUE排序为优一(2.35 mm o l/m o l)>龙王帽(2.29 mm o l/m o l)>一窝蜂(2.22 mm o l/m o l)。3个品种比较而言,一窝蜂和龙王帽属高光合、强蒸腾品种,适宜栽植在水分和光照条件较好的地方;优一属弱蒸腾、低光合品种,适于在光照和水分条件稍差的地方生长。展开更多
【目的】研究黄土高原不同县域山杏种子可溶性蛋白质含量和电泳谱带的差异,为山杏种内分类及良种选育提供理论依据。【方法】采用形态学观测法,对黄土高原(陕西麟游和甘肃灵台、华池、庆城)山杏种质资源变异情况进行调查,从中筛选出形...【目的】研究黄土高原不同县域山杏种子可溶性蛋白质含量和电泳谱带的差异,为山杏种内分类及良种选育提供理论依据。【方法】采用形态学观测法,对黄土高原(陕西麟游和甘肃灵台、华池、庆城)山杏种质资源变异情况进行调查,从中筛选出形态类型典型的山杏,采用考马斯亮蓝G-250染色法和SDS-PAGE电泳法对种子可溶性蛋白质含量和谱带进行检测分析。【结果】共筛选出了37种山杏变异类型,其种子可溶性蛋白质含量差异较大。SDS-PAGE电泳分析表明,山杏种子可溶性蛋白质含量丰富、多态、稳定、谱带清晰,主要由41.1,39.0,34.6,22.7,22.0和21.7 ku 6种亚基组成,其余亚基数量丰富但含量较少。聚类分析表明,37种山杏类型可分为5个大类。【结论】种子可溶性蛋白质分类结果与形态特征和生物学特性存在一定的相似性,利用分类结果可对山杏品种进行改良。展开更多
基金Supported by Start-up Fund for High-level Scientific Research Personnel(11GK30)
文摘[ Objective] The aim was to establish the optimal ISSR-PCR system for Armeniaca sibirica. [ Method] With Armeniaca sibirica as experimental materi- al, genomic DNA was extracted from tender leaves using CTAB method. Orthogonal experiment L9 （34） was conducted with four factors （Mg2＋ , dNTPs, primers, Taq DNA polymerase） in ISSR-PCR system ofArmeniaca sibirica at three levels. The results of orthogonal experimental design were analyzed by using SPSS statisti- cal software. [Result] Different levels of various factors all had significant effects on PCR results, specifically, the amount of Taq DNA polymerase had the most significant effect. The optimal ISSR-PCR reaction system of Armeniaca sibirica was established ： the total PCR reaction volume was 20 ~1, containing 1.0 U of Taq DNA polymerase, 1.0 mmol/L MgCI2,0.15 mmol/L dNTPs and 0.30 ~mol/L primers. Validation result shows that this reaction system is very stable and strongly operational. [ Conclusion] This study laid the foundation for genetic diversity analysis and germplasm resource investigation of Arnniaca sibirica.
文摘对陕西吴旗县引种栽培的龙王帽、一窝蜂和优一3个大扁杏品种的光响应曲线、净光合速率(P n)、蒸腾速率(T r)、气孔导度(G s)和水分利用效率(WUE)等生理特性指标进行了测定和比较分析。结果表明:龙王帽、一窝蜂和优一的光饱和点分别为600,600和400μm o l/(m2.s);日均P n排序为一窝蜂[4.07μm o l/(m2.s)]>龙王帽[3.75μm o l/(m2.s)]>优一[3.17μm o l/(m2.s)],日均T r排序为一窝蜂[1.74 mm o l/(m2.s)]>龙王帽[1.51mm o l/(m2.s)]>优一[1.29 mm o l/(m2.s)],日均WUE排序为优一(2.35 mm o l/m o l)>龙王帽(2.29 mm o l/m o l)>一窝蜂(2.22 mm o l/m o l)。3个品种比较而言,一窝蜂和龙王帽属高光合、强蒸腾品种,适宜栽植在水分和光照条件较好的地方;优一属弱蒸腾、低光合品种,适于在光照和水分条件稍差的地方生长。
文摘【目的】研究黄土高原不同县域山杏种子可溶性蛋白质含量和电泳谱带的差异,为山杏种内分类及良种选育提供理论依据。【方法】采用形态学观测法,对黄土高原(陕西麟游和甘肃灵台、华池、庆城)山杏种质资源变异情况进行调查,从中筛选出形态类型典型的山杏,采用考马斯亮蓝G-250染色法和SDS-PAGE电泳法对种子可溶性蛋白质含量和谱带进行检测分析。【结果】共筛选出了37种山杏变异类型,其种子可溶性蛋白质含量差异较大。SDS-PAGE电泳分析表明,山杏种子可溶性蛋白质含量丰富、多态、稳定、谱带清晰,主要由41.1,39.0,34.6,22.7,22.0和21.7 ku 6种亚基组成,其余亚基数量丰富但含量较少。聚类分析表明,37种山杏类型可分为5个大类。【结论】种子可溶性蛋白质分类结果与形态特征和生物学特性存在一定的相似性,利用分类结果可对山杏品种进行改良。
