[Objectives]To analyze the genetic similarity among varieties of Armeniaca vulgaris Shushanggan in Ili.[Methods]The genetic distance among 13 A.vulgaris Shushanggan varieties was compared at the DNA molecular level,an...[Objectives]To analyze the genetic similarity among varieties of Armeniaca vulgaris Shushanggan in Ili.[Methods]The genetic distance among 13 A.vulgaris Shushanggan varieties was compared at the DNA molecular level,and clustering analysis was performed on them.[Results]The 13 varieties of A.vulgaris Shushanggan were classified into four clusters.The first cluster included Shushang Ganxing 1,Shushang Ganxing 4,Gongliu Yexing and Zhenzhu Youxing,and the similarity coefficient between Shushang Ganxing 1 and Shushang Ganxing4 reached 0.808;the second cluster were Shushang Ganxing 2,Shushang Ganxing 3 and Liguangxing,and the similarity coefficient between Shushang Ganxing 2 and Shushang Ganxing 3 was 0.846[1];Ili Baixing,61 Tuan Guyexing,Chaxian Yexing and Huocheng Yexing were classified into the third cluster,and the similarity coefficient between Ili Baixing and 61 Tuan Guyexing was 0.692;and the fourth cluster included Shushang Ganxing W1 and Yixian Yexing,between which the similarity coefficient was 0.692.[Conclusions]This will lay a foundation for the research of variety identification and genetic structure of A.vulgaris Shushanggan.展开更多
[ Objective] The aim was to establish the optimal ISSR-PCR system for Armeniaca sibirica. [ Method] With Armeniaca sibirica as experimental materi- al, genomic DNA was extracted from tender leaves using CTAB method. O...[ Objective] The aim was to establish the optimal ISSR-PCR system for Armeniaca sibirica. [ Method] With Armeniaca sibirica as experimental materi- al, genomic DNA was extracted from tender leaves using CTAB method. Orthogonal experiment L9 （34） was conducted with four factors （Mg2＋ , dNTPs, primers, Taq DNA polymerase） in ISSR-PCR system ofArmeniaca sibirica at three levels. The results of orthogonal experimental design were analyzed by using SPSS statisti- cal software. [Result] Different levels of various factors all had significant effects on PCR results, specifically, the amount of Taq DNA polymerase had the most significant effect. The optimal ISSR-PCR reaction system of Armeniaca sibirica was established ： the total PCR reaction volume was 20 ~1, containing 1.0 U of Taq DNA polymerase, 1.0 mmol/L MgCI2,0.15 mmol/L dNTPs and 0.30 ~mol/L primers. Validation result shows that this reaction system is very stable and strongly operational. [ Conclusion] This study laid the foundation for genetic diversity analysis and germplasm resource investigation of Arnniaca sibirica.展开更多
文摘[Objectives]To analyze the genetic similarity among varieties of Armeniaca vulgaris Shushanggan in Ili.[Methods]The genetic distance among 13 A.vulgaris Shushanggan varieties was compared at the DNA molecular level,and clustering analysis was performed on them.[Results]The 13 varieties of A.vulgaris Shushanggan were classified into four clusters.The first cluster included Shushang Ganxing 1,Shushang Ganxing 4,Gongliu Yexing and Zhenzhu Youxing,and the similarity coefficient between Shushang Ganxing 1 and Shushang Ganxing4 reached 0.808;the second cluster were Shushang Ganxing 2,Shushang Ganxing 3 and Liguangxing,and the similarity coefficient between Shushang Ganxing 2 and Shushang Ganxing 3 was 0.846[1];Ili Baixing,61 Tuan Guyexing,Chaxian Yexing and Huocheng Yexing were classified into the third cluster,and the similarity coefficient between Ili Baixing and 61 Tuan Guyexing was 0.692;and the fourth cluster included Shushang Ganxing W1 and Yixian Yexing,between which the similarity coefficient was 0.692.[Conclusions]This will lay a foundation for the research of variety identification and genetic structure of A.vulgaris Shushanggan.
基金Supported by Start-up Fund for High-level Scientific Research Personnel(11GK30)
文摘[ Objective] The aim was to establish the optimal ISSR-PCR system for Armeniaca sibirica. [ Method] With Armeniaca sibirica as experimental materi- al, genomic DNA was extracted from tender leaves using CTAB method. Orthogonal experiment L9 （34） was conducted with four factors （Mg2＋ , dNTPs, primers, Taq DNA polymerase） in ISSR-PCR system ofArmeniaca sibirica at three levels. The results of orthogonal experimental design were analyzed by using SPSS statisti- cal software. [Result] Different levels of various factors all had significant effects on PCR results, specifically, the amount of Taq DNA polymerase had the most significant effect. The optimal ISSR-PCR reaction system of Armeniaca sibirica was established ： the total PCR reaction volume was 20 ~1, containing 1.0 U of Taq DNA polymerase, 1.0 mmol/L MgCI2,0.15 mmol/L dNTPs and 0.30 ~mol/L primers. Validation result shows that this reaction system is very stable and strongly operational. [ Conclusion] This study laid the foundation for genetic diversity analysis and germplasm resource investigation of Arnniaca sibirica.
