Objective:To study the chemical constituents of the EtOAc extract of Armillaria gallica 012m.Methods:The chemical constituents of the EtOAc extract of A.gallica 012m were isolated and purified by various column chroma...Objective:To study the chemical constituents of the EtOAc extract of Armillaria gallica 012m.Methods:The chemical constituents of the EtOAc extract of A.gallica 012m were isolated and purified by various column chromatography and their structures were elucidated on the basis of the 1D and 2D NMR spectroscopic and HRESIMS data.Cytotoxicity of all isolates against A549,HCT-116,M231 and W256 human tumor cells was determined by the MTT method.Results:A new sesquiterpene aryl ester,armimelleolide C(1),and eight known ones including armillarivin(2),melleolide F(3),6'-chloromelleolide F(4),melleolide(5),melleolide K(6),melledonol(7),13-hydroxydihydromelleolide(8),and armillane(9),were isolated from the EtOAc extract of A.gallica 012m.All isolates showed potential cytotoxic activities against at least one of the human cancer cell lines with IC_(50) values ranging from(3.17±0.54)to(17.57±0.47)μmol/L.Compound 1 showed significant inhibitory activity against M231 with an IC_(50) value of(7.54±0.24)μmol/L compared with paclitaxel as the positive control.Compounds 2,3,and 7,9 showed obvious inhibitory activity against HCT-116 and were better than that of the positive control.Conclusion:The chemical constituents including a new sesquiterpene aryl ester armimelleolide C(1)from the EtOAc extract of A.gallica 012m have a variety of structures and potential antitumor activities.展开更多
基金This project was in part supported financially by National Natural Science Foundation of China(Nos.31960095 and 82160670)National Natural Science Foundation of Yunnan Province(Nos.202101AS070022 and 202201AT070228)Yunnan Ten thousand Talents Program to Weiguang Wang and Yanping Li.
文摘Objective:To study the chemical constituents of the EtOAc extract of Armillaria gallica 012m.Methods:The chemical constituents of the EtOAc extract of A.gallica 012m were isolated and purified by various column chromatography and their structures were elucidated on the basis of the 1D and 2D NMR spectroscopic and HRESIMS data.Cytotoxicity of all isolates against A549,HCT-116,M231 and W256 human tumor cells was determined by the MTT method.Results:A new sesquiterpene aryl ester,armimelleolide C(1),and eight known ones including armillarivin(2),melleolide F(3),6'-chloromelleolide F(4),melleolide(5),melleolide K(6),melledonol(7),13-hydroxydihydromelleolide(8),and armillane(9),were isolated from the EtOAc extract of A.gallica 012m.All isolates showed potential cytotoxic activities against at least one of the human cancer cell lines with IC_(50) values ranging from(3.17±0.54)to(17.57±0.47)μmol/L.Compound 1 showed significant inhibitory activity against M231 with an IC_(50) value of(7.54±0.24)μmol/L compared with paclitaxel as the positive control.Compounds 2,3,and 7,9 showed obvious inhibitory activity against HCT-116 and were better than that of the positive control.Conclusion:The chemical constituents including a new sesquiterpene aryl ester armimelleolide C(1)from the EtOAc extract of A.gallica 012m have a variety of structures and potential antitumor activities.
文摘高卢蜜环菌(Armillaria gallica)为北半球广布种,不同大陆间的菌株遗传相似性和多样性水平能反映出该种在洲际大陆尺度上的地理遗传变异关系。作者用ISSR(inter-simple sequence repeat)分子标记技术,对从中国和欧洲收集到的高卢蜜环菌79个菌株进行了遗传多样性分析。用6个ISSR引物扩增得到210个位点,其中多态性位点(频率<0.95)为202个,占96.2%,平均每个引物多态位点多达33.6个,表明ISSR标记在蜜环菌中存在较高的多态性。根据非加权类平均法(UPGMA)聚类分析,中国53个菌株中的49个在0.773的相似性水平上聚成了中国类群(China group);而欧洲26个菌株遗传分化较大,分别在0.775和0.763的相似性水平上聚成了欧洲类群A(Europe group A)和B(Europe group B);2个欧洲类群间的相似性水平仅为0.738,而欧洲类群A与中国类群间的相似性却达到了0.770;两个大陆均有少数菌株表现出较为明显的遗传分化,个别菌株的种内遗传相似性甚至低于蜜环菌种间的遗传相似性。结果表明,中欧两个大陆间的A.gallica菌株因地理隔离已经表现出明显的遗传分化,处于异域物种形成过程中;欧洲大陆的菌株遗传分化更为明显,可能是两个大陆A.gallica菌株的起源地。
