Optimization for Microbial Degumming of Ramie with Bacillus subtilis DZ_(5) in Submerged Fermentation by Orthogonal Array Design and Response Surface Methodology

As a kind of natural fiber,ramie fiber has distinctive advantages in textile application,but the application is limited due to the traditional degumming mode.Compared with the traditional degumming process,the microbial degumming process has many advantages.To obtain the optimal conditions for degumming ramie with Bacillus subtilis DZ_(5)(BS DZ_(5)),a combined statistical approach of orthogonal array design(OAD)and response surface methodology(RSM)was used.The influences of initial pH of the bacteria medium,culture temperature,shaking speed,degumming time and inoculum size on submerged fermentation degumming were evaluated by using fractional factorial design.The main factors in the analysis were culture temperature,shaking speed and initial pH.The residual gum mass fraction was used as the optimization index,and the optimal conditions for degumming were determined by central composite design and RSM.Thus with only a limited number of experiments,an optimal ramie microbial degumming condition was found as the culture temperature of 40℃,the initial pH in the culture medium of 8.5,the shaking speed of 205 r/min,the degumming time of 96 h and the inoculum size of 5%.After microbial degumming of ramie under the optimal conditions,there was only 10.6%residual gum by mass in the fiber.In addition,the effective degumming of BS DZ_(5)was also confirmed by a scanning electron microscope(SEM).

Objective array design for three-dimensional temperature and salinity observation:Application to the South China Sea

In this study,a moored array optimization tool(MAOT)was developed and applied to the South China Sea(SCS)with a focus on three-dimensional temperature and salinity observations.Application of the MAOT involves two steps:(1)deriving a set of optimal arrays that are independent of each other for different variables at different depths based on an empirical orthogonal function method,and(2)consolidating these arrays using a K-center clustering algorithm.Compared with the assumed initial array consisting of 17 mooring sites located on a 3°×3°horizontal grid,the consolidated array improved the observing ability for three-dimensional temperature and salinity in the SCS with optimization efficiencies of 19.03%and 21.38%,respectively.Experiments with an increased number of moored sites showed that the most cost-effective option is a total of 20 moorings,improving the observing ability with optimization efficiencies up to 26.54%for temperature and 27.25%for salinity.The design of an objective array relies on the ocean phenomenon of interest and its spatial and temporal scales.In this study,we focus on basin-scale variations in temperature and salinity in the SCS,and thus our consolidated array may not well resolve mesoscale processes.The MAOT can be extended to include other variables and multi-scale variability and can be applied to other regions.

Optimization of Supercritical Fluid Extraction and Rapid Resolution LC-MS/ESI Identification of Chromones from Saposhnikoviae Radix through Orthogonal Array Design

Objective To establish a rapid and effective supercritical fluid extraction(SFE) and rapid resolution liquid chromatography method coupled with diode-array detector(RRLC-DAD) to quantify the chromones in a species. Methods The effects of four parameters including ethanol concentration(50%-90%), pressure(25-45 MPa), temperature(40-60 ℃), and time(30-90 min) on the chromones yields, namely prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, and sec-O-glucosylhamaudol, were investigated using SFE system with orthogonal array design(OAD). Furthermore, the extracts were analyzed using rapid resolution liquid chromatography coupled with diode-array detector(RRLC-DAD) system to confirm the results. Results Under the optimized conditions, i.e., 35 MPa of pressure, 60 ℃ of temperature, 70% ethanol, and 60 min of time, the yields of prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, sec-O-glucosylhamaudol, and total chromones were 3.514, 0.132, 6.242, 0.342, and 10.231 mg/g, respectively. In comparison with ultrasonic assisted extraction(UAE), SFE was able to yield a 20.7% increase in the total chromones from Saposhnikoviae Radix. Conclusion SFE is an alternative and promising method to extract chromones from this species, and the established RRLC-DAD method could serve as a rapid and effective method for the identification of chromones from Saposhnikoviae Radix.

Design of nano-piezoelectric immunosensor array to detect bone sialoprotein

Objective With the unique properties of Au nano-particles,a nano-piezoelectric immunosensor microarray was designed using AT-cut 10 Hz quartz crystal to detect blood sialoprotein (BSP) expression from clinical serum.Methods