Purpose： The objective of this study was to investigate the anti-tumor effects and analyze the mechanism of artesunate （ART） action on breast cancer in vivo using tumor transplanted nude mice. Methods： The human b...Purpose： The objective of this study was to investigate the anti-tumor effects and analyze the mechanism of artesunate （ART） action on breast cancer in vivo using tumor transplanted nude mice. Methods： The human breast tumor cell line MCF-7 was transplanted into nude mice, and the animals were treated with various doses of ART alone or in combination with cyclophosphamide （CTX） or normal saline （NS）. The tumor inhibitory effects were observed and compared, and the ultrastructural morphology of the transplanted tumor cells was observed by electron microscopy. The apoptosis rates and cell cycle status were detected by flow cytometry （FCM）. The expression of apoptosis-related proteins p53, Bcl-2, Bax and Caspase-3 were detected by immunohistochemistry and IGF-IR was detected by western blot. The expression correlation for these proteins was also analyzed. Results： The tumor inhibition rates in the low dose ART group, high dose ART group, CTX group and combined drug therapy group were （24.39±10.20）%, （40.24±7.02）%, （57.01±5.84）% and （68.29±5.1）%, respectively. The cell cycle was arrested in phase G0/Gt after treatment with ART. The expression of Bcl-2 was significantly reduced, and the expression levels of Bax and Caspase-3 were significantly increased in the ART group compared to the negative control saline group. There was no significant difference detected in p53 expression. The Bcl-2 level was negatively related to Bax and Caspase-3. The western blotting results showed IGF-IR downregulation. Conclusions： ART inhibits the growth of MCF-7 breast tumor cell xenografts in nude mice. The anti-tumor mechanism of ART for human breast carcinoma in nude mice might be correlated with the alteration of apoptosis related protein expression, which may further induce apoptosis and inhibit cell proliferation.展开更多
Rabies is caused by infection of rabies virus(RABV)and remains a serious threat to the global public health.Except for the requirement for cold chain and high cost of human rabies immune globulin,no small molecule dru...Rabies is caused by infection of rabies virus(RABV)and remains a serious threat to the global public health.Except for the requirement for cold chain and high cost of human rabies immune globulin,no small molecule drugs are currently available for clinical treatment of rabies.So,it is of great importance to identify novel compounds that can effectively inhibit RABV infection.Artesunate(ART)and dihydroartemisinin(DHA),two derivatives of artemisinin,are widely used for treatment of malaria in adults and children,showing high safety.In this study,we found that both ART and DHA were able to inhibit RABV replication in host cells at a low concentration(0.1μmol/L).The antiviral effects of ART and DHA were independent of viral strains and cell lines.Pre-treatment with ART or DHA for 2 h in vitro did not affect the viral replication in host cells,implying that ART and DHA neither reduced the viability of RABV directly nor inhibited the binding and entrance of the virus to host cells.Further studies revealed that ART and DHA inhibited RABV genomic RNA synthesis and viral gene transcription.Treatment with ART or DHA(5 mg/kg)by intramuscular injection improved,to some extent,the survival rate of RABV-challenged mice.Combination treatment with derivatives of artemisinin and mannitol significantly improved the survival rate of RABV-challenged mice.The results suggest that ART and DHA have a great potential to be explored as new anti-rabies agents for treatment of rabies.展开更多
To celebrate the publication of a single-page article published40 years ago,the Chinese Science Bulletin recently published a special issue with a collection of 11 papers covering subjects ranging from the history of ...To celebrate the publication of a single-page article published40 years ago,the Chinese Science Bulletin recently published a special issue with a collection of 11 papers covering subjects ranging from the history of artemisinin(ART)discovery to recent advances in ART research.Published with the articles are introductories from the journal’s editor-in-chief,Dr.Fu Gao,and the director of展开更多
将一种产品做到极致,是许多伟大设计师的追求。在今年iF设计奖的获奖名单中,有一家设计公司可以说是把一件日常生活中不太起眼的产品做到了极致——其6件获奖作品中有5件是马桶冲水按钮,其中的Visign for More 202更荣获了2020年iF金奖...将一种产品做到极致,是许多伟大设计师的追求。在今年iF设计奖的获奖名单中,有一家设计公司可以说是把一件日常生活中不太起眼的产品做到了极致——其6件获奖作品中有5件是马桶冲水按钮,其中的Visign for More 202更荣获了2020年iF金奖。这件极简设计的金奖作品背后,是来自德国的ARTEFAKT Design。展开更多
Research on fires at the wildland-urban inter-face(WUI)has generated significant insights and advance-ments across various fields of study.Environmental,agri-culture,and social sciences have played prominent roles in ...Research on fires at the wildland-urban inter-face(WUI)has generated significant insights and advance-ments across various fields of study.Environmental,agri-culture,and social sciences have played prominent roles in understanding the impacts of fires in the environment,in protecting communities,and addressing management challenges.This study aimed to create a database using a text mining technique for global researchers interested in WUI-projects and highlighting the interest of countries in this field.Author’s-Keywords analysis emphasized the dominance of fire science-related terms,especially related to WUI,and identified keyword clusters related to the WUI fire-risk-assessment-system-“exposure”,“danger”,and“vulnerability”within wildfire research.Trends over the past decade showcase shifting research interests with a growing focus on WUI fires,while regional variations highlighted that the“exposure”keyword cluster received greater atten-tion in the southern Europe and South America.However,vulnerability keywords have relatively a lower representation across all regions.The analysis underscores the interdisci-plinary nature of WUI research and emphasizes the need for targeted approaches to address the unique challenges of the wildland-urban interface.Overall,this study provides valu-able insights for researchers and serves as a foundation for further collaboration in this field through the understanding of the trends over recent years and in different regions.展开更多
The continuous progress of urbanization has driven the continuous development and innovation of landscape planning and design.Focused on the important design method of modern construction art,this study analyzed its c...The continuous progress of urbanization has driven the continuous development and innovation of landscape planning and design.Focused on the important design method of modern construction art,this study analyzed its concepts and characteristics,and made deep exploration to its application in landscape planning and design.The results indicated that modern construction art had a significant impact on landscape spatial planning and layout,spatial design forms,and spatial ornaments.The use of modern construction art concepts could make landscape design more scientific,artistic,and humane,creating higher quality leisure and entertainment venues for audiences.展开更多
This study was aimed to explore the feasibility of introducing two mainstream AI drawing applications,Midjournal and Stable Diffusion,into the environmental art and design courses in higher education institutions.On t...This study was aimed to explore the feasibility of introducing two mainstream AI drawing applications,Midjournal and Stable Diffusion,into the environmental art and design courses in higher education institutions.On the basis of introducing the development history of AI drawing technology,the characteristics of Midjournal and Stable Diffusion and their applications in teaching were described in detail.The analysis on its practical application in public space design courses showed that AI drawing technology could significantly improve students’creative efficiency and the possibility of artistic creation,while also enriching the expressive power of design.In spite of the challenges such as technical accuracy,response to complex design requirements,hardware dependencies,and student dependencies,the application of AI drawing technology had an overall positive prospects in the field of education.Finally,the value of AI drawing technology in teaching was emphasized,and the challenges it brought were discussed,as well as how to fully utilize this technology while maintaining teaching objectives and educational principles.展开更多
Context and objective: Around 8% of incident cases of tuberculosis (TB) were reported among people living with HIV worldwide in 2022. Tuberculosis is the leading cause of death among people living with HIV. Africa acc...Context and objective: Around 8% of incident cases of tuberculosis (TB) were reported among people living with HIV worldwide in 2022. Tuberculosis is the leading cause of death among people living with HIV. Africa accounts for the majority of co-infection episodes, with over 50% of cases in some parts of southern Africa. In the Democratic Republic of Congo (DRC), around 9% of persons living with HIV (PLHIV) develop TB and 11% of TB patients are infected with HIV. The DRC is one of the 30 countries in the world bearing the brunt of co-infection. Despite the efforts made by countries to improve access to antiretroviral traitement (ART), TB remains a major problem among people living with HIV. The Lingwala Health Zone in the provincial city of Kinshasa recorded a large number of cases of HIV/TB co-infection during the study period. The aim of this study was to determine the factors associated with HIV/TB co-infection among PLHIV on ART in the Lingwala health zone (HZ) in Kinshasa. Methods: This was a case-control study conducted in the state-run HIV care facilities in the Lingwala health district among PLHIV who had visited the health facilities during the period 2021-2023. Cases were coinfected patients and controls were PLHIV who had not developed tuberculosis during the study period. Results: A total of 281 PLHIV were enrolled in the study, with 70 cases and 211 controls. Factors associated with HIV/TB co-infection after multivariate analysis were viral load (OR = 5.34;95% CI;1.8-15.8, p = 0.005). History of tuberculosis (OR = 20.84;95% CI;8.6-50.3, p -85.0, p = 0.005) and BMI Conclusion: The results of this study indicate that the detection of these enumerated factors should prompt providers to actively search for tuberculosis with a view to organising early management.展开更多
基金Province Science Fund for Young Scholars (No. QC05C46)Science Foundation from Health Bureau of Heilongjiang Province (No. 2005-47)
文摘Purpose： The objective of this study was to investigate the anti-tumor effects and analyze the mechanism of artesunate （ART） action on breast cancer in vivo using tumor transplanted nude mice. Methods： The human breast tumor cell line MCF-7 was transplanted into nude mice, and the animals were treated with various doses of ART alone or in combination with cyclophosphamide （CTX） or normal saline （NS）. The tumor inhibitory effects were observed and compared, and the ultrastructural morphology of the transplanted tumor cells was observed by electron microscopy. The apoptosis rates and cell cycle status were detected by flow cytometry （FCM）. The expression of apoptosis-related proteins p53, Bcl-2, Bax and Caspase-3 were detected by immunohistochemistry and IGF-IR was detected by western blot. The expression correlation for these proteins was also analyzed. Results： The tumor inhibition rates in the low dose ART group, high dose ART group, CTX group and combined drug therapy group were （24.39±10.20）%, （40.24±7.02）%, （57.01±5.84）% and （68.29±5.1）%, respectively. The cell cycle was arrested in phase G0/Gt after treatment with ART. The expression of Bcl-2 was significantly reduced, and the expression levels of Bax and Caspase-3 were significantly increased in the ART group compared to the negative control saline group. There was no significant difference detected in p53 expression. The Bcl-2 level was negatively related to Bax and Caspase-3. The western blotting results showed IGF-IR downregulation. Conclusions： ART inhibits the growth of MCF-7 breast tumor cell xenografts in nude mice. The anti-tumor mechanism of ART for human breast carcinoma in nude mice might be correlated with the alteration of apoptosis related protein expression, which may further induce apoptosis and inhibit cell proliferation.
基金We thank Dr.Xianzhu Xia(Academy of Military Medical Sciences)for providing the CVS-11 virus.We thank Dr.Zhenfang Fu(Huazhong Agricultural University)for providing the CVS-24 virus.This work was supported by the National Key Research and Development Program of China(2016YFD0500400)National Natural Science Foundation of China(31772742)Natural Science Foundation of Guangdong(2015A03031103).
文摘Rabies is caused by infection of rabies virus(RABV)and remains a serious threat to the global public health.Except for the requirement for cold chain and high cost of human rabies immune globulin,no small molecule drugs are currently available for clinical treatment of rabies.So,it is of great importance to identify novel compounds that can effectively inhibit RABV infection.Artesunate(ART)and dihydroartemisinin(DHA),two derivatives of artemisinin,are widely used for treatment of malaria in adults and children,showing high safety.In this study,we found that both ART and DHA were able to inhibit RABV replication in host cells at a low concentration(0.1μmol/L).The antiviral effects of ART and DHA were independent of viral strains and cell lines.Pre-treatment with ART or DHA for 2 h in vitro did not affect the viral replication in host cells,implying that ART and DHA neither reduced the viability of RABV directly nor inhibited the binding and entrance of the virus to host cells.Further studies revealed that ART and DHA inhibited RABV genomic RNA synthesis and viral gene transcription.Treatment with ART or DHA(5 mg/kg)by intramuscular injection improved,to some extent,the survival rate of RABV-challenged mice.Combination treatment with derivatives of artemisinin and mannitol significantly improved the survival rate of RABV-challenged mice.The results suggest that ART and DHA have a great potential to be explored as new anti-rabies agents for treatment of rabies.
基金supported by the Division of Intramural Research,National Institute of Allergy and Infectious Diseases(NIAID),USA
文摘To celebrate the publication of a single-page article published40 years ago,the Chinese Science Bulletin recently published a special issue with a collection of 11 papers covering subjects ranging from the history of artemisinin(ART)discovery to recent advances in ART research.Published with the articles are introductories from the journal’s editor-in-chief,Dr.Fu Gao,and the director of
文摘将一种产品做到极致,是许多伟大设计师的追求。在今年iF设计奖的获奖名单中,有一家设计公司可以说是把一件日常生活中不太起眼的产品做到了极致——其6件获奖作品中有5件是马桶冲水按钮,其中的Visign for More 202更荣获了2020年iF金奖。这件极简设计的金奖作品背后,是来自德国的ARTEFAKT Design。
基金The funding of this research was provided by the Portuguese Foundation for Science and Technology(FCT)in the framework of the House Refuge Project(PCIF/AGT/0109/2018).
文摘Research on fires at the wildland-urban inter-face(WUI)has generated significant insights and advance-ments across various fields of study.Environmental,agri-culture,and social sciences have played prominent roles in understanding the impacts of fires in the environment,in protecting communities,and addressing management challenges.This study aimed to create a database using a text mining technique for global researchers interested in WUI-projects and highlighting the interest of countries in this field.Author’s-Keywords analysis emphasized the dominance of fire science-related terms,especially related to WUI,and identified keyword clusters related to the WUI fire-risk-assessment-system-“exposure”,“danger”,and“vulnerability”within wildfire research.Trends over the past decade showcase shifting research interests with a growing focus on WUI fires,while regional variations highlighted that the“exposure”keyword cluster received greater atten-tion in the southern Europe and South America.However,vulnerability keywords have relatively a lower representation across all regions.The analysis underscores the interdisci-plinary nature of WUI research and emphasizes the need for targeted approaches to address the unique challenges of the wildland-urban interface.Overall,this study provides valu-able insights for researchers and serves as a foundation for further collaboration in this field through the understanding of the trends over recent years and in different regions.
基金Sponsored by Germplasm Collection and Conservation Project for the Forest and Grass Germplasm Resources in Anhui Province in 2024(hxkt2024111)Science and Technology Plan Project of Huangshan(2022KN-02)+1 种基金Humanities and Social Sciences Research Project of Anhui Higher Education Institutions(SKHS2019B07)Key School-level Project of Huangshan University(2022xkjzd004).
文摘The continuous progress of urbanization has driven the continuous development and innovation of landscape planning and design.Focused on the important design method of modern construction art,this study analyzed its concepts and characteristics,and made deep exploration to its application in landscape planning and design.The results indicated that modern construction art had a significant impact on landscape spatial planning and layout,spatial design forms,and spatial ornaments.The use of modern construction art concepts could make landscape design more scientific,artistic,and humane,creating higher quality leisure and entertainment venues for audiences.
基金Sponsored by Educational and Teaching Reform Projects in 2023 at North China University of Technology.
文摘This study was aimed to explore the feasibility of introducing two mainstream AI drawing applications,Midjournal and Stable Diffusion,into the environmental art and design courses in higher education institutions.On the basis of introducing the development history of AI drawing technology,the characteristics of Midjournal and Stable Diffusion and their applications in teaching were described in detail.The analysis on its practical application in public space design courses showed that AI drawing technology could significantly improve students’creative efficiency and the possibility of artistic creation,while also enriching the expressive power of design.In spite of the challenges such as technical accuracy,response to complex design requirements,hardware dependencies,and student dependencies,the application of AI drawing technology had an overall positive prospects in the field of education.Finally,the value of AI drawing technology in teaching was emphasized,and the challenges it brought were discussed,as well as how to fully utilize this technology while maintaining teaching objectives and educational principles.
文摘Context and objective: Around 8% of incident cases of tuberculosis (TB) were reported among people living with HIV worldwide in 2022. Tuberculosis is the leading cause of death among people living with HIV. Africa accounts for the majority of co-infection episodes, with over 50% of cases in some parts of southern Africa. In the Democratic Republic of Congo (DRC), around 9% of persons living with HIV (PLHIV) develop TB and 11% of TB patients are infected with HIV. The DRC is one of the 30 countries in the world bearing the brunt of co-infection. Despite the efforts made by countries to improve access to antiretroviral traitement (ART), TB remains a major problem among people living with HIV. The Lingwala Health Zone in the provincial city of Kinshasa recorded a large number of cases of HIV/TB co-infection during the study period. The aim of this study was to determine the factors associated with HIV/TB co-infection among PLHIV on ART in the Lingwala health zone (HZ) in Kinshasa. Methods: This was a case-control study conducted in the state-run HIV care facilities in the Lingwala health district among PLHIV who had visited the health facilities during the period 2021-2023. Cases were coinfected patients and controls were PLHIV who had not developed tuberculosis during the study period. Results: A total of 281 PLHIV were enrolled in the study, with 70 cases and 211 controls. Factors associated with HIV/TB co-infection after multivariate analysis were viral load (OR = 5.34;95% CI;1.8-15.8, p = 0.005). History of tuberculosis (OR = 20.84;95% CI;8.6-50.3, p -85.0, p = 0.005) and BMI Conclusion: The results of this study indicate that the detection of these enumerated factors should prompt providers to actively search for tuberculosis with a view to organising early management.
