“渝青蒿2号”新品种选育研究

目的:选育高产、优质的青蒿新品种。方法:以青蒿栽培群体特异性种质资源为材料,选择优株,以形态特征、农艺性状和青蒿素含量为指标,观察历代选育材料,进行品比试验和区域试验,选优去劣,育成新品种。结果:选育的青蒿新品种"渝青蒿2号",平均干叶产量为2 346.86 kg/hm^2,比对照(生产种渝青1号)增产7.01%;平均青蒿素含量为12.66‰,比对照提高15.51%,为高产优质新品种。结论:自交系单株选择法可明显提高青蒿叶产量和青蒿素含量,选育的"渝青蒿2号"具有良好的推广价值。

青蒿组织培养再生体系的建立

为了建立和优化青蒿的组织培养再生体系,获得快速繁殖技术,以青蒿叶片和叶柄为外植体,研究了不同激素配比对其离体培养和大量快速繁殖的影响。结果表明:以青蒿叶片为外植体,其愈伤组织诱导培养基为MS+2.0mg/L 6-BA+0.5mg/L 2,4-D,诱导率为78.0%;分化培养基为MS+1.0mg/L 6-BA+0.05mg/L NAA+3%蔗糖+0.7%琼脂粉,分化率达95.3%。分化苗在1/2MS+1.0mg/L NAA培养基上快速生根,且根系发达,粗壮,生根率达98.9%。以青蒿叶柄为外殖体,叶柄可在培养基MS+2.0mg/L 6-BA+0.5mg/L 2,4-D上一次成苗,在1/2MS+1.0mg/L NAA培养基上生根良好。该体系有效的缩短了培养周期,具有重要的研究价值。

Studies on the biosynthesis of arteannuin——Ⅳ.The biosynthesis of arteanniuin and arteannuin B by the leaf homogenate of Artemisia annua L.

Arteannuic acid and(-)11R,13-d(?)ydroarteannuic acid are the key intermediates in the biosynthesis of arteannuin by the leaf homogenate of Artemisia annua L.,and arteannuic acid and epoxyarteannuic acid are the intermediates of arteannuin B.and epoxyarteannuic acid can not be transformed into arteannuin by the homogenate.