Artemisinin is a novel effective antimalarial drug extracted from the medicinal plant Artemisia annua L. Owing to the tight market and low yield of artemisinin, there is great interest in enhancing the production of a...Artemisinin is a novel effective antimalarial drug extracted from the medicinal plant Artemisia annua L. Owing to the tight market and low yield of artemisinin, there is great interest in enhancing the production of artemisinin. In the present study, farnesyi diphosphate synthase （FPS） was overexpressed in high-yield A. annua to Increase the artemisinin content. The FPS activity in transgenic A. ennue was twoto threefold greater than that In non-transgenic A. annua. The highest artemisinin content in transgenic A. annua was approximately 0.9% （dry weight）, which was 34.4% higher than that in non-transgenic A. annua. The results demonstrate the regulatory role of FPS in artemisinin biosynthesis.展开更多
Pre-column derivation of and high-performance liquid chromatography(HPLC)with ultraviolet detection were used to detect the epurated artemisinin in Artemisia annua,and the artemisinin content and biomass yield were co...Pre-column derivation of and high-performance liquid chromatography(HPLC)with ultraviolet detection were used to detect the epurated artemisinin in Artemisia annua,and the artemisinin content and biomass yield were compared between the introduced A.annua and the local wild A.annua.The result showed that the quantity of artemisinin was mainly affected by the genetic background,and secondly influenced by the environment and climate of the cultivated region.The seedlings raised with the integrated plate-type paper containers method in greenhouse had longer growth period of A.annua by about 45 days in frigid area.The highest content of artemisinin was 0.555% and the highest biomass yield was(3 526.7±378)kg·hm-2.High artemisinin content and biomass yield together contributed to artimisinin production.The introduced cultivars had a strong capacity to quickly adapt to the circumstance in Heilongjiang.The artemisinin contents reached to the purchasing level.The mean biomass of individual plant was greater than that in its original region.展开更多
基金the National Natural Science Foundation of China （30171142）.
文摘Artemisinin is a novel effective antimalarial drug extracted from the medicinal plant Artemisia annua L. Owing to the tight market and low yield of artemisinin, there is great interest in enhancing the production of artemisinin. In the present study, farnesyi diphosphate synthase （FPS） was overexpressed in high-yield A. annua to Increase the artemisinin content. The FPS activity in transgenic A. ennue was twoto threefold greater than that In non-transgenic A. annua. The highest artemisinin content in transgenic A. annua was approximately 0.9% （dry weight）, which was 34.4% higher than that in non-transgenic A. annua. The results demonstrate the regulatory role of FPS in artemisinin biosynthesis.
文摘Pre-column derivation of and high-performance liquid chromatography(HPLC)with ultraviolet detection were used to detect the epurated artemisinin in Artemisia annua,and the artemisinin content and biomass yield were compared between the introduced A.annua and the local wild A.annua.The result showed that the quantity of artemisinin was mainly affected by the genetic background,and secondly influenced by the environment and climate of the cultivated region.The seedlings raised with the integrated plate-type paper containers method in greenhouse had longer growth period of A.annua by about 45 days in frigid area.The highest content of artemisinin was 0.555% and the highest biomass yield was(3 526.7±378)kg·hm-2.High artemisinin content and biomass yield together contributed to artimisinin production.The introduced cultivars had a strong capacity to quickly adapt to the circumstance in Heilongjiang.The artemisinin contents reached to the purchasing level.The mean biomass of individual plant was greater than that in its original region.
