Impact of Inulin Extracted, Purified from (Chicory and Globe Artichoke) Roots and the Combination with Maltodextrin as Prebiotic Dietary Fiber on the Functional Properties of Stirred Bio-Yogurt

Inulin is a prebiotic dietary fiber that plays an integral role in producing functional dairy products with improved health benefits. Therefore, the objectives of this study are as follows: extract and purify inulin from chicory roots and globe artichoke roots;evaluate the physicochemical, functional properties and functional groups of the purified inulin;determine the functional properties of chicory roots inulin-maltodextrin and globe artichoke roots inulin-maltodextrin and compare it with that of the commercial inulin;examine the impact of various inulin on physiochemical, microstructural, textural, sensory characteristics and as prebiotic dietary fiber on probiotic bacteria’s viability of stirred bio-yogurt. The characteristics of the microstructure were investigated by scanning electron microscopy and, Fourier transforms infrared spectroscopy to detect the functional group. The resulting inulin exhibited a high yield and purity along with enhanced functional properties. Stirred bio-yogurt fortified with chicory roots inulin or globe artichoke roots inulin showed enhanced physicochemical, microstructural, microbiological, and overall sensorial acceptability followed by chicory roots inulin-maltodextrin or globe artichoke roots inulin-maltodextrin and the commercial inulin as compared to the control. Stirred bio-yogurt samples can offer various health benefits and wide applications as supplement of prebiotic dietary fiber in dairy industry.

Protective Role of Ca Against NaCl Toxicity in Jerusalem Artichoke by Up-Regulation of Antioxidant Enzymes

The ameliorative effect of external Ca^2＋ on Jerusalem artichoke （Helianthus tuberosus L.） under salt stress was studied through biochemical and physiological analyses of Jerusalem artichoke seedlings treated with or without 10 mol L^-1 CaCl2, 150 mmol L^-1 NaCl, and/or 5 mmol L^-1 ethylene-bis（oxyethylenenitrilo）-tetraacetic acid （EGTA） for five days. Exposure to NaC1 （150 mmol L^-1） decreased growth, leaf chlorophyll content, and photosynthetic rate of Jerusalem artichoke seedlings. NaC1 treatment showed 59% and 37% higher lipid peroxidation and electrolyte leakage, respectively, than the control. The activities of superoxide dismutase （SOD）, peroxidase （POD）, and catalase （CAT） were decreased by NaCl, indicating an impeded antioxidant defense mechanism of Jerusalem artichoke grown under salt stress. Addition of 10 mmol L^-1 CaCl2 to the salt solutions significantly decreased the damaging effect of NaC1 on growth and chlorophyll content and simultaneously restored the rate of photosynthesis almost to the level of the control. Ca^2＋ addition decreased the leaf malondialdehyde （MDA） content and electrolyte leakage from NaCl-treated seedlings by 47% and 24%, respectively, and significantly improved the activities of SOD, POD, and CAT in NaCl-treated plants. Addition of EGTA, a specific chelator of Ca2＋, decreased the growth, chlorophyll content, and photosynthesis, and increased level of MDA and electrolyte leakage from NaCl-treated plants and from the control plants. EGTA addition to the growth medium also repressed the activities of SOD, POD, and CAT in NaCl-treated and control seedlings. External Ca2＋ might protect Jerusalem artichoke against NaC1 stress by up-regulating the activities of antioxidant enzymes and thereby decreasing the oxidative stress.

Effect of Saline Aquaculture Effluent on Salt-Tolerant Jerusalem Artichoke (Helianthus tuberosus L.) in a Semi-Arid Coastal Area of China

An experiment with six treatments: CK1 (rainfed), CK2 (irrigated with freshwater), and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1:1, 1:2, 1:3, and 1:4 (v/v) was carried out during 2004 to assess the effect of saline aquaculture effluent on plant growth and soil properties in the Laizhou region, Shandong Province, China and to determine an optimal salinity threshold for aquaculture effluent. Cumulative evapotranspiration for the saline aquaculture effluent irrigation and non-irrigation treatments was lower than that for the freshwater irrigation treatment. Soil electrical conductivity was higher with respect to saline aquaculture effluent irrigation treatment compared to that with respect to non-irrigation or freshwater irrigation treatment. For Jerusalem artichoke (Helianthus tuberosus L.), in comparison to the freshwater treatment, plant height and aboveground biomass for the 1:3 and 1:4 treatments were constrained, whereas stem width and root biomass were enhanced. Concomitantly, higher tuber yield was obtained for the 1:3 and 1:4 treatments compared to that for CK1 and 1:1 treatments. Nitrogen and phosphorus were higher in tubers of the 1:4 treatment. This study demonstrated that saline aquaculture effluent could be used successfully to irrigate Jerusalem artichoke with higher tuber yield and nutrient removal.

Soil Properties and Yield of Jerusalem Artichoke(Helianthus tuberosus L.)with Seawater Irrigation in North China Plain

Irrigation with various dilutions of seawater can act as an alternate water resource and thus plays an important role in saving freshwater resources as well as promoting agriculture in the coastal semi-arid areas of the North China Plain. Jerusalem artichoke （Helianthus tuberosus L.） grown in a field experiment was irrigated with seawater diluted with freshwater from 2001 to 2003 to determine the feasibility of seawater irrigation in the Laizhou area. For treatments of CK （non-irrigation） along with seawater concentrations of 25%, 50%, and 75%, total dissolved solid （TDS） in the non-irrigated soil significantly increased （P ≤ 0.05） in both 2002 and 2003 and was 1.3 times higher in 2003 than in 2001. In the 25% and 50% seawater concentration treatments, TDS in 2001 was significantly greater （P ≤ 0.05） than CK; however, TDS in these two treatments decreased by 34.9% and 40.1%, respectively, in 2003 compared with 2001. The sodium adsorption ratio （SAR） remained below 10 mmol^1/2 L^-1/2, indicating that alkalization was low with seawater irrigation. In 2001 and 2002, compared to CK and the irrigation treatment with 75% seawater, irrigation with 25% and 50% seawater increased the yields of Jerusalem .artichoke. This meant that Jerusalem artichoke could be safely grown in salt-affected land of Laizhou area with 25% and 50% seawater irrigation.

Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth

The removal of solid impurities and separation of target products from a fermentation broth is becoming more tedious with the utilization of lignocelluloses as source of substrate.2,3-Butanediol,an important chemical used widely is also a main product of sugar-based fermentation carried out by Klebsiella pneumoniae.In this study,we investigated the use of salting-out extraction(SOE) that employed a K2HPO4/ethanol system consisting of 21% ethanol and 17% K2HPO4(mass fraction) to separate 2,3-butanediol from the viscous Jerusalem artichoke-based fermentation broth.After SOE,about 98% of solid matters was removed,and the viscosity decreased from 72.5 mPa s in the original fermentation broth to 4.4 mPa s in the top phase.The partition coefficient and yield of 2,3-butanediol reached 13.4 and 99%,respectively,and 89% of soluble proteins was removed from the broth.The results showed that SOE is an efficient way for isolating 2,3-BD from a highly viscous fermentation broth by removing much of the solid matters within the broth.

Optimization of pectin extraction and antioxidant activities from Jerusalem artichoke

Jerusalem artichoke is an economic crop widely planted in saline-alkaline soil. The use of Jerusalem artichoke is of great significance. In this study, the response surface method was employed to optimize the effects of processing variables （extraction temperature, pH, extraction time, and liquid-to- solid ratio） on the yield of Jerusalem artichoke pectin. Under the optimal extraction conditions： pHl .52, 63.62 min, 100℃ and a liquid-to-solid ratio of 44.4 mL/g, the maximum pectin yield was predicted to be 18.76%. Experiments were conducted under these optimal conditions and a pectin yield of 18.52＋0.90% was obtained, which validated the model prediction. The effects of different drying methods （freeze drying, spray drying and vacuum drying） on the properties of Jerusalem artichoke pectin were evaluated and they were compared with apple pectin. FTIR spectral analysis showed no major structural differences in Jerusalem artichoke pectin samples produced by various drying treatments. The antioxidant activities of pectin dried by different methods were investigated using in vitro hydroxyl and DPPH radical scavenging systems. The results revealed that the activities of spray dried pectin （SDP） and apple pectin （AP） were stronger than those of vacuum oven dried pectin （ODP） and vacuum freeze dried pectin （FDP）. Therefore compared with the other two drying methods, the spray drying method was the best.

Assessing Genetic Structure and Relatedness of Jerusalem Artichoke (<i>Helianthus tuberosus</i>L.) Germplasm with RAPD, ISSR and SRAP Markers

Jerusalem artichoke (Helianthus tuberosus L.) is an old tuber crop with a recently renewed interest in multipurpose improvement, but little effort has been made to characterize its genetic resources. A study was conducted to assess genetic structure and genetic relatedness of 47 diverse Jerusalem artichoke accessions using RAPD, ISSR and SRAP markers. A total of 296 (87.1%) polymorphic bands were detected from 13 RAPD markers;92 (80%) from six ISSR primers;and 194 (88.6%) for nine combinations of SRAP primers. Five optimal clusters were inferred by the STRUCTURE program from the RAPD or ISSR data, while six optimal clusters were found from the SRAP data or combined marker data. Significant linear relationships between the distance matrices for all pairs of individual accessions were detected for all marker pairs and the estimated correlation coefficient was 0.40 for RAPD-ISSR, 0.53 for RAPD-SRAP, and 0.43 for ISSR-SRAP. Based on the combined data, the neighbor-joining clustering of the 47 accessions matched closely with those inferred from the STRUCTURE program. Three ancestral groups were observed for the Canadian germplasm. Most diverse germplasm harbored in the USA collection. These findings not only reveal the compatible patterns of genetic structure and relatedness inferred with three marker types, but also are useful for managing Jerusalem artichoke germplasm and utilizing diverse germplasm for genetic improvement.

Study on Accumulation and Dynamic Changes of Three Carbohydrates in Jerusalem Artichoke in Natural Habitats

Two varieties of Jerusalem artichoke (early maturing variety Qingyu No.1 and middle maturing variety Qingyu No.2),which were bred independently by Research and Development Center of Jerusalem Artichoke,Qinghai Academy of Agriculture and Forestry,were used as materials,and the content and dynamic changes of three kinds of carbohydrates (sucrose,glucose and fructose) in various organs of Jerusalem artichoke in different periods in two natural habitats (water plain and low hill dry land) were studied.The results showed that the content of the three kinds of carbohydrates in Qingyu No.1 and Qingyu No.2 in the water plain and low hill dry land was relatively high in late June and late August.The total content of the three carbohydrates in Qingyu No.1 peaked earlier than that of Qingyu No.2.The total content of the three carbohydrates in the water plain peaked earlier than that of the low hill dry land.In the whole growth period,the total content of the three carbohydrates in the stems and roots of Jerusalem artichoke was higher than that of the leaves.In the leaves,fructose accumulation was relatively obvious in the whole growth period,and the content was high,followed by glucose.In the stems and roots,fructose content was high,followed by sucrose.In the tubers,glucose accumulation was obvious,and sucrose content was lower than glucose and fructose content.When the tubers were harvested,fructose content was high.

Biomass Accumulation and Nutrient Uptake of Jerusalem Artichoke (<i>Helianthus tuberosus</i>L.)

The dynamics of biomass accumulation during the growing period, the yield of leafy stalks and tubers, and the nutrient concentration and nutrient uptake of the yield were investigated for two Jerusalem artichoke varieties (Tápiói Korai and Tápiói Sima) in a field experiment involving mineral fertilisation. Considerable differences were observed between the dynamics of leafy stalk and tuber development in Tápiói Korai which has a short vegetation period and Tápiói Sima where the vegetation period is long. The maximum dry matter ratio between the tuber yield and the leafy stalk yield was 1:1 for Tápiói Korai and 1:4.5 for Tápiói Sima. During the period when the maximum aboveground biomass developed in Tápiói Korai, 100 kg.ha-1 N and P fertiliser resulted in the highest leafy stalk yield (38.34 t.ha-1), while for Tápiói Sima, which developed a much greater leafy stalk mass, the highest aboveground biomass yield (78-80 t.ha-1) was given in response to 200 kg.ha-1 N supplemented by P and K fertiliser. Both artichoke varieties produced the great-est tuber yield at a N rate of 200 kg.ha-1, supplemented with P and K fertiliser. The nutrient concentration in the leafy stalks was highest on the 85th day of the vegetation period, prior to intensive dry matter accumulation in the leafy stalks and before tuber formation began. In both varieties the maximum nutrient uptake was recorded on the 155th day. Great differences were observed between the varieties in terms of specific nutrient uptake. For a tuber yield of 10 t, together with the corresponding leafy stalk yield, the specific nutrient uptake of the Tápiói Korai variety amounted to 48 kg N, 10 kg P, 83 kg K, 30 kg Ca and 10 kg Mg, while for Tápiói Sima these figures were 162 kg N, 30 kg P, 300 kg K, 84 kg Ca and 45 kg Mg.

Polyphenol Composition, Antioxidant, Antimicrobial and Quorum Quenching Activity of the “Carciofo di Montoro” (<i>Cynara cardunculus</i>var. <i>scolymus</i>) Global Artichoke of the Campania Region, Southern Italy

Biochemical characteristics, antimicrobial and quorum quenching activity of the extract of the “Carciofo di Montoro”, a typical ecotype of Cynara cardunculus var. scolymus of the Campania region (Southern Italy) were studied, to consider it as potential reserve of bioactive constituents useful for food industry and beneficial for managing and preventing several chronic illnesses in humans. The extract exhibited a good polyphenol content (528 μg GAE/g) and antioxidant activity (EC50 less than 5 mg). Ultra pressure liquid chromatography (UPLC) revealed high amount of chlorogenic acid, cynarin and epicatechin. The extract showed antimicrobial activity against Escherichia coli, Staphylocccus aureus, Pseudomonas aeruginosa, Enterococcus faecalis and Bacillus cereus pathogen strains. Finally, quorum quenching activity was demonstrated. The variety Carciofo di Montoro could represent a good source of health-promoting polyphenols, encouraging a nutraceutical use of such ecotype, for several phyto-pharmaceutical applications.

Biological Studies on Bio-Yoghurt Fortified with Prebiotic Obtained from <i>Jerusalem artichoke</i>

Inulin, an oligosaccharide produced by several plants, has been shown to enhance the viability of probiotic cultures in milk through storage. Jerusalem artichoke ( Helianthus tuberosus L.) is an interested prebiotic because its tuber has risen content of inulin and fructo-oligosaccharides. This study was aimed to: 1) set the effect of Jerusalem artichoke in deferent concentrations (2.5% & 5%) on the growth of probiotic Lb. acidophilus P106 in the bio-yoghurt during cold storage at 5℃ and sensory evaluation of probiotic yoghurts;2) study the effect of feeding with this synbiotic fermented milk on diabetic mice. It could be concluded that the Jerusalem artichoke influenced the growth of Lb. acidophilus P106 and 5% (w/v) Jerusalem artichoke was given the highest growth and sensory evaluation. On the other hand, no serious adverse effects were observed;the reduction of blood glucose was observed at the termination of empirical phase, also, high level (5%) of Jerusalem artichoke led to more reduction of blood glucose, cholesterol levels and total lipids compared with control.

Extraction and Powder Product of Fructo-oligosaccharide from Jerusalem Artichoke

Fructo-oligosaccharide （FOS） is a type of dietary fiber. It can not be digested by enzymes in the body. It also helps to improve a digestion in the intestine, an excretion system and prevent the cancer. Jerusalem artichoke is scanned and analyzed by High Performance Liquid Chromatography （HPLC） for quantity of FOS. They are selected for extraction and drying process. Studies on the extraction for a powder making, the conditions are optimum for maximum yield. Experiments manage using a factorial real 2 × 4 × 6 in 2 blocks of Randomized Complete Block Design （RCBD） models. The first factor studies on two types of solvent. The second factor is the four levels of temperature in the extraction with water at 25 ℃, 35 ℃, 50℃ and 60℃ for 30 min. The third factor is the ratio of sample per solvent as 5, 6, 10, 15, 20 and 30 times. The data and comparison of average is analyzed by Duncan＇s New Mutiple Range Test at the significant level 0.05. The concentrated extracts are processed to powder by freeze drying, a hot air and vacuum drying. Optimal conditions control the temperature and time by heating in both vacuum and conventional oven. The results of temperature on the extraction are significantly different at a = 0.05. In drying process with high temperature, the color of product is more yellow （b） and less white （L）. The difference of color value is statistically significant at level a = 0.05. The sensory evaluation of food products are added the extracted powder and attribute by panelist. High level acceptance of product is aspectual about texture and flavor. FOS is the trend of functional foods for health. It is focus on low energy and on obesity including increasing the absorption of calcium.

Screening of Yeasts for Fermentation of Jerusalem Artichoke Tubers Juice and Selection of the Active Strains for Ethanol Production

Strains K.marxianus Y-303,Oenoferm credo,Oenoferm rouge,Sacch.vini Rkaseteli-6,Sacch.cerevisiae XII and Sacch.uvarum 73 were used for fermentation of juice,extracted from Jerusalem artichoke tubers grown in Uzbekistan.The fermentation process was carried out in laboratory conditions.Among these yeasts Oenoferm credo,Oenoferm rouge and Sacch.uvarum 73 showed higher performance than K.marxianus Y-303 and the ethanol yields were 92%,90%and 88%,respectively.After 72 h fermentation with Sacch.vini Rkaseteli-6 and Sacch.cerevisiae XII the yield of ethanol was 82%and 74%of maximum theoretical yield,respectively.

Chips Production from Jerusalem Artichoke(Helianthus tuberosus L.)

Jerusalem artichoke has been cultivated in various regions without any special breeding technique. As a food, Jerusalem artichoke has a characteristic flavour and functional ingredients including inulin, other dietary fibers, minerals and traces of polyphenol. The production of chips from Jerusalem artichoke was examined in this study because it contains high amount of inulin. Inulin gives health promoting effects especially for the people suffering from diabetes. Firstly,chips production from Jerusalem artichoke was studied in the scope of properties like moisture, oil, color, texture, sensory. After cleaning, tubers prepared in specified size and slices were cooked in the deep fat fryer or microwave oven. The best results obtained for frying and microwave oven application of Jerusalem artichoke were obtained at 180℃ for 240s treatment time and at 600 W for105s, respectively.

菊芋功能成分及其在畜牧生产中的应用进展

菊芋具有产量高、适应性强、应用范围广的特点,富含菊糖、黄酮、绿原酸及氨基酸等活性物质。菊芋具有改善畜禽肠道生态环境、缓解便秘、促进矿物质吸收、调节血糖、血脂等作用,可降低家畜死亡率,提高屠宰率、肉品质、生产性能和免疫器官指数,具备作为功能性饲料添加剂的潜质。文章主要综述了菊芋菊糖、绿原酸、黄酮及氨基酸的理化性质、生理功能及其在动物生产中的应用,以期为菊芋资源作为功能性饲料添加剂在畜牧生产中的推广应用提供参考。

基于MaxEnt模型的陕西省菊芋种植潜在适宜区分析

利用陕西省29个菊芋空间分布数据和26个环境因子,综合陕西省边际土地分布,运用最大熵模型(MaxEnt)模拟历史时期及未来气候变化下陕西省菊芋潜在适宜区分布,并将结果叠加陕西省现有边际土地进行分析。结果表明:(1)MaxEnt模型模拟陕西省菊芋种植潜在适宜区结果精度较高,曲线下面积AUC为0.914。(2)基于历史时期环境数据,陕西省菊芋潜在分布区主要分布在榆林定边县、延安志丹县、安康汉滨区、商洛商州区和汉中西乡县,边际土地总适宜区面积约为75992km^(2),占陕西省总面积的36.9%,其中低、中、高适宜性面积分别约为63169、11617和1205km^(2),主要边际土地利用类型为中覆盖度草地。(3)影响菊芋生长的5个重要环境因子为土壤深度(142.4~155.4cm)、最暖月最高气温(29.4~31.6℃)、高程(210~593m)、坡向(70.7°~239.5°)和坡度(0~10.5°)。(4)未来4种气候情景模式下陕西省菊芋种植中、高适宜区面积总体明显增加,2021-2040年分别增加14.39%~46.46%和46.90%~95.39%,2041-2060年分别增加15.95%~71.52%和48.46%~111.40%。未来气候变化有助于陕西菊芋适宜种植区扩大,建议在榆林定边县、商洛商州区等高适宜地区开展种植实验,验证菊芋对当地环境的适宜性,探索推广培育的可行性。

牧草型菊芋新品种廊芋25号饲用营养品质研究

为了实现牧草型菊芋新品种选育充实菊芋种质资源库,试验以从俄罗斯引进的菊芋种质资源R4中选育成的牧草型菊芋新品种廊芋25号及廊芋19号、廊芋20号、廊芋21号、廊芋22号、廊芋23号、廊芋24号为供试育种材料,以青芋2号为对照品种,进行了产量与营养品质测定;以鲜秸秆和块茎产量为目标进行同异比较分析,并对廊芋25号菊芋秸秆粉和菊芋全粉进行营养成分测定。结果表明:廊芋25号茎叶粗蛋白含量为20.41%,比青芋2号高68.68%;茎叶粗脂肪和粗纤维含量均最高,分别为3.79%、34.88%;鲜秸秆产量为5565.86 kg/亩(1亩≈667 m^(2)),块茎产量为3521.05 kg/亩,分别比青芋2号高26.21%、63.02%。以廊芋25号鲜秸秆和块茎产量为目标的同异联系度均最高,分别为0.8170,0.8600。廊芋25号菊芋秸秆粉干物质含量高,并富含中性洗涤纤维(≥53.00%);全粉中含有糖(65.200%)、粗蛋白(9.810%)、微量元素及16种氨基酸,还含有少量黄酮。说明廊芋25号是一个优良的牧草型特性菊芋新品种,适宜作为畜禽日粮原料。

山药红枣菊芋糕加工工艺研究

为拓宽糕点工业化发展的道路,更好地满足人们对糕点口味和营养多样化的需求,本文将山药泥、菊芋泥、糯米粉混合成面团作为糕点皮,以红枣泥为馅,采用蒸制的方法,通过单因素和正交试验优化山药红枣菊芋糕的加工工艺。结果表明,山药红枣菊芋糕的最佳工艺为山药泥12 g、菊芋泥8 g、红枣泥15 g、白砂糖3 g。在此条件下制作的山药红枣菊芋糕气味纯正,口感细腻,香甜可口,具有良好的感官品质,理化指标和微生物指标符合食品安全国家标准。

菊芋新品种青芋7号的选育

青芋7号是由河南地方优良菊芋农家种经单株系统选育而成的加工型中晚熟菊芋新品种。全生育期175 d(天)左右,株高160~200 cm,分枝数17~22个;块茎呈瘤状,分布较集中,外皮红色,肉白色,平均块茎质量36.2 g,表面光滑,须根少;干物质含量192.0 g · kg^(-1),可溶性糖含量57.0 g · kg^(-1),粗蛋白含量20.6 g · kg^(-1),粗纤维含量6.7 g · kg^(-1),川水地栽培每667 m2产量可达5 800 kg,适宜在青海省川水地、浅山地种植。

黑菊芋加工工艺设备优化与验证

为解决传统黑菊芋加工设备工序繁杂、加工周期长、加工成本高的问题,设计结构合理、可监测温湿度及气压的小型试验箱。采用控制变量法,改变试验箱开孔密度,对菊芋进行分组加工试验,结合感官评价和营养成分分析确定最佳生产条件。再以此条件对传统小型黑菊芋加工设备进行改进,对传统加工程序进行优化。改造后的黑菊芋加工设备工序减少50%,加工时间减少45%,各项生产费用节省了83%。改造后的的黑菊芋加工设备生产效率明显提升,生产成本大幅下降;同时降低了塑料制品的使用,节能减排、低碳环保。