Research Progress on Artificial Cultivation of Trhicoloma Matsutake and Theory of the Shiro in Changbai Mountains

Study on artificial cultivation of Tricholoma matsutake carried through by the present research group in Changbai Mountains in recent years was briefly reviewed in the paper, and then, the signifycance of cultivation. Mechanism of formation, hiberarchy, grouwth cycle and suitable living environment, were summarized.

Successful Cultivation of Albino Mauremys mutica for the First Time

Artificial albino Mauremys mutica was bred by a wild female albino M.mutica(P).F1 progeny was bred by mating between albino female turtle(P)and common male turtle,and F2 progeny was bred by mating between mature F1 male turtle with albino female turtle(P).After 7-8 years of cultivation,the artificial albino M.mutica was successfully produced from the F3 progeny bred in 2007,which was the first artificial breeding of the albino M.mutica.Through the cultivation of 18 batches of 5 generations(F5),the emergence rate of albino M.mutica reached more than 85%,and it could reach 100%in some cases.A stable albino M.mutica gene was produced for the first time.

Screening of Strains and Nutrition Sources for Culture of Armillariella mellea Rhizomoph Branch in Ganzi

Based on the natural resources in Ganzi Tibetan Autonomous Prefecture, screening experiment of strains and nutrient sources for culture of Armillariella mellea rhizomoph branch was carried out with 3 strains of A. mellea, 2 types of main nutrient sources and 3 kinds of auxiliary nutrient sources. Total 18 treatments were designed. The results showed that with the A. mellea strain separated from the tubers of wild Gastrodia elata BI. by tissue isolation as mother strain, the shrubs such as Prunus tatsienensis Batal and Quercus spinosa David ex Franch as the main nutrient sources, and the agricultural products such as corn and potato as the supplementary nutrient sources, the growth of A. mellea rhizomoph branch was best in bottle.

Prospects for Development of Edible and Medicinal Mycorrhizal Fungus in Shanxi Province

This paper first elaborated basic characteristics and research development of the edible mycorrhizal fungus. Then,it analyzed the natural ecological environment and development conditions of edible fungus in Shanxi Province. Finally,it discussed edible mycorrhizal fungus resources in Shanxi Province and development and utilization prospects of edible mycorrhizal fungi,to provide some references for the development of edible fungi industry and edible and medicinal mycorrhizal fungus in Shanxi Province and adjacent provinces.

Discovery of differential sequences for improving breeding and yield of cultivated Ophiocordyceps sinensis through ITS sequencing and phylogenetic analysis

To accelerate the breeding process of cultivated Ophiocordyceps sinensis and increase its yield,it is important to identify molecular fingerprint of dominant O.sinensis.In the present study,we collected 3 batches of industrially cultivated O.sinensis product with higher yield than the others and compared their internal transcribed spacer(ITS)sequences with the wild and the reported.The ITS sequence was obtained by bidirectional sequencing and analyzed with molecular systematics as a DNA barcode for rapid and accurate identification of wild and cultivated O.sinensis collected.The ITS sequences of O.sinensis with detailed collection loci on NCBI were downloaded to construct a phylogenetic tree together with the sequences obtained from the present study by using neighbor-joining method based on their evolution relationship.The information on collection loci was analyzed with ArcGIS 10.2 to demonstrate the geographic distribution of these samples and thus to determine the origin of the dominant samples.The results showed that all wild and cultivated samples were identified as O.sinensis and all sequences were divided into seven phylogenetic groups in the tree.Those groups were precisely distributed on the map and the process of their system evolution was clearly presented.The three cultivated samples were clustered into two dominant groups,showing the correlation between the industrially cultivated samples and the dominant wild samples,which can provide references for its optimized breeding in the future.