AIM:To investigate the effects of Chrysanthemum indicum extract(CIE)on inhibition of proliferation and on apoptosis,and the underlying mechanisms,in a human hepatocellular carcinoma(HCC)MHCC97H cell line. METHODS:Viab...AIM:To investigate the effects of Chrysanthemum indicum extract(CIE)on inhibition of proliferation and on apoptosis,and the underlying mechanisms,in a human hepatocellular carcinoma(HCC)MHCC97H cell line. METHODS:Viable rat hepatocytes and human endothelial ECV304 cells were examined by trypan blue exclusion and MTT assay,respectively,as normal controls.The proliferation of MHCC97H cells was determined by MTT assay.The cellular morphology of MHCC97H cells was observed by phase contrast microscopy.Flow cytometry was performed to analyze cell apoptosis with annexin V/propidium iodide(PI), mitochondrial membrane potential with rhodamine 123 and cell cycle with PI in MHCC97H cells.Apoptotic proteins such as cytochrome C,caspase-9,caspase-3and cell cycle proteins,including P21 and CDK4,were measured by Western blotting. RESULTS:CIE inhibited proliferation of MHCC97H cells in a time-and dose-dependent manner without cytotoxicity in rat hepatocytes and human endothelial cells.CIE induced apoptosis of MHCC97H cells in a concentration-dependent manner,as determined by flow cytometry.The apoptosis was accompanied by a decrease in mitochondrial membrane potential, release of cytochrome C and activation of caspase-9 and caspase-3.CIE arrested the cell cycle in the S phase by increasing P21 and decreasing CDK4 protein expression. CONCLUSION:CIE exerted a significant apoptotic effect through a mitochondrial pathway and arrested the cell cycle by regulation of cell cycle-related proteins in MHCC97H cells without an effect on normal cells. The cancer-specific selectivity shown in this study suggests that the plant extract could be a promising novel treatment for human cancer.展开更多
Pharmacodynamics material basis and effective mechanisms are the two main issues to decipher the mechnisms of action of Traditional Chinese medicines(TCMs)for the treatment of diseases.TCMs,in“multi-component,multi-t...Pharmacodynamics material basis and effective mechanisms are the two main issues to decipher the mechnisms of action of Traditional Chinese medicines(TCMs)for the treatment of diseases.TCMs,in“multi-component,multi-target,multi-pathway”paradigm,show satisfactory clinical results in complex diseases.New ideas and methods are urgently needed to explain the complex interactions between TCMs and diseases.Network pharmacology(NP)provides a novel paradigm to uncover and visualize the underlying interaction networks of TCMs against multifactorial diseases.The development and application of NP has promoted the safety,efficacy,and mechanism investigations of TCMs,which then reinforces the credibility and popularity of TCMs.The current organcentricity of medicine and the“one disease-one target-one drug”dogma obstruct the understanding of complex diseases and the development of effective drugs.Therefore,more attentions should be paid to shift from“phenotype and symptom”to“endotype and cause”in understanding and redefining current diseases.In the past two decades,with the advent of advanced and intelligent technologies(such as metabolomics,proteomics,transcriptomics,single-cell omics,and artificial intelligence),NP has been improved and deeply implemented,and presented its great value and potential as the next drug-discovery paradigm.NP is developed to cure causal mechanisms instead of treating symptoms.This review briefly summarizes the recent research progress on NP application in TCMs for efficacy research,mechanism elucidation,target prediction,safety evaluation,drug repurposing,and drug design.展开更多
目的:观察中药熏眼联合雷火灸治疗干眼症的临床疗效。方法:选取2015年3月至2016年8月广东省第二中医院眼科门诊干眼症患者120例(共240只眼),随机分为人工泪液+中药熏眼+雷火灸(A组),人工泪液对照组(B组),人工泪液+中药熏眼对照组(C组),...目的:观察中药熏眼联合雷火灸治疗干眼症的临床疗效。方法:选取2015年3月至2016年8月广东省第二中医院眼科门诊干眼症患者120例(共240只眼),随机分为人工泪液+中药熏眼+雷火灸(A组),人工泪液对照组(B组),人工泪液+中药熏眼对照组(C组),人工泪液+雷火灸对照组(D组),每组各30例(60只眼)。A组予以自拟中药熏蒸双眼,1次/d;雷火灸熏双眼,1次/d;羟丙甲纤维素滴眼液点双眼,4次/d。B组予以羟丙甲纤维素滴眼液点双眼,4次/d。C组予以自拟中药熏蒸双眼,1次/d;羟丙甲纤维素滴眼液点双眼,D组予以雷火灸熏双眼,1次/d;羟丙甲纤维素滴眼液点双眼,4次/d。疗程均为10 d,观察4组患者治疗前后的临床症状评分、泪膜破裂时间(BUT)、泪液分泌实验(Schirmer test I)、角膜荧光素染色(FL),比较4组临床效果。结果:A组治疗有效率为96.7%,B组治疗有效率为60.0%,C组治疗有效率均为80.0%,D组治疗有效率均为83.3%,A组与B、C、D组间比较差异有统计学意义(P<0.05)。A组的临床症状评分、BUT、Schirmer test I评分、FL评分与B、C、D组间比较,差异均有统计学意义(P<0.05)结论:在人工泪液治疗干眼症的基础上结合中药熏眼、雷火灸能够获得较显著的治疗效果。展开更多
目的研究中药人工周期联合地屈孕酮片对宫内膜异位症(EMs)不孕患者妊娠及血清学的影响。方法选取2014年3月至2016年3月间在南通市中医院接受治疗的EMs不孕患者96例,依据随机数表法将患者分成两组:对照组(48例)使用地屈孕酮片治疗,观察组...目的研究中药人工周期联合地屈孕酮片对宫内膜异位症(EMs)不孕患者妊娠及血清学的影响。方法选取2014年3月至2016年3月间在南通市中医院接受治疗的EMs不孕患者96例,依据随机数表法将患者分成两组:对照组(48例)使用地屈孕酮片治疗,观察组(48例)在对照组基础上结合中药治疗;观察患者治疗后妊娠情况、治疗前后血清肿瘤坏死因子-α(TNF-α)、白介素6(IL-6)水平变化情况,使用B超检测患者A型子宫内膜数量,卵泡发育状况,子宫内膜的厚度和排卵日内卵巢血流动力学情况。结果观察组和对照组患者妊娠率分别为54.17%、37.50%,差异有统计学意义(P<0.05);治疗后观察组和对照组患者血清TNF-α[(17.45±4.26)ng/m L vs(22.07±4.36)ng/m L]、IL-6[(18.24±3.16)pg/m L vs(23.17±3.50)pg/m L]、舒张期末流速(EDV)[(11.02±1.43)cm/s vs(9.30±1.86)cm/s]、搏动指数(PI)[(0.69±0.31)vs(1.21±1.05)]、收缩期峰值流速(PSV)[(21.10±1.89)cm/s vs(18.24±1.93)cm/s]比较,差异均有统计学意义(P<0.05)。结论中药人工周期联合地屈孕酮片可改善患者卵泡和子宫内膜的发育与生长,降低炎症因子水平,提高妊娠率。展开更多
基金Supported by Grants From the National Natural Science Foundation of China,No.30672766Science and Technology Developing Foundation of Shaanxi Province,China,No.2006K16-G4(1)
文摘AIM:To investigate the effects of Chrysanthemum indicum extract(CIE)on inhibition of proliferation and on apoptosis,and the underlying mechanisms,in a human hepatocellular carcinoma(HCC)MHCC97H cell line. METHODS:Viable rat hepatocytes and human endothelial ECV304 cells were examined by trypan blue exclusion and MTT assay,respectively,as normal controls.The proliferation of MHCC97H cells was determined by MTT assay.The cellular morphology of MHCC97H cells was observed by phase contrast microscopy.Flow cytometry was performed to analyze cell apoptosis with annexin V/propidium iodide(PI), mitochondrial membrane potential with rhodamine 123 and cell cycle with PI in MHCC97H cells.Apoptotic proteins such as cytochrome C,caspase-9,caspase-3and cell cycle proteins,including P21 and CDK4,were measured by Western blotting. RESULTS:CIE inhibited proliferation of MHCC97H cells in a time-and dose-dependent manner without cytotoxicity in rat hepatocytes and human endothelial cells.CIE induced apoptosis of MHCC97H cells in a concentration-dependent manner,as determined by flow cytometry.The apoptosis was accompanied by a decrease in mitochondrial membrane potential, release of cytochrome C and activation of caspase-9 and caspase-3.CIE arrested the cell cycle in the S phase by increasing P21 and decreasing CDK4 protein expression. CONCLUSION:CIE exerted a significant apoptotic effect through a mitochondrial pathway and arrested the cell cycle by regulation of cell cycle-related proteins in MHCC97H cells without an effect on normal cells. The cancer-specific selectivity shown in this study suggests that the plant extract could be a promising novel treatment for human cancer.
基金the Natural Science Foundation of Zhejiang Province(No.LZ20H290002)the Innovation Team and Talents Cultivation Program of the National Administration of Traditional Chinese Medicine(No.ZYYCXTD-D-202002)+2 种基金the Fundamental Research Funds for the Central Universities(No.226-2022-00226)the Science and Technological Innovation Project for College Students in Zhejiang Province(Xinmiao Talent Plan 2022R424A005)Zhejiang Provincial Administration of Traditional Chinese Medicine(Nos.2022ZQ022,2023ZF083).
文摘Pharmacodynamics material basis and effective mechanisms are the two main issues to decipher the mechnisms of action of Traditional Chinese medicines(TCMs)for the treatment of diseases.TCMs,in“multi-component,multi-target,multi-pathway”paradigm,show satisfactory clinical results in complex diseases.New ideas and methods are urgently needed to explain the complex interactions between TCMs and diseases.Network pharmacology(NP)provides a novel paradigm to uncover and visualize the underlying interaction networks of TCMs against multifactorial diseases.The development and application of NP has promoted the safety,efficacy,and mechanism investigations of TCMs,which then reinforces the credibility and popularity of TCMs.The current organcentricity of medicine and the“one disease-one target-one drug”dogma obstruct the understanding of complex diseases and the development of effective drugs.Therefore,more attentions should be paid to shift from“phenotype and symptom”to“endotype and cause”in understanding and redefining current diseases.In the past two decades,with the advent of advanced and intelligent technologies(such as metabolomics,proteomics,transcriptomics,single-cell omics,and artificial intelligence),NP has been improved and deeply implemented,and presented its great value and potential as the next drug-discovery paradigm.NP is developed to cure causal mechanisms instead of treating symptoms.This review briefly summarizes the recent research progress on NP application in TCMs for efficacy research,mechanism elucidation,target prediction,safety evaluation,drug repurposing,and drug design.
文摘目的:观察中药熏眼联合雷火灸治疗干眼症的临床疗效。方法:选取2015年3月至2016年8月广东省第二中医院眼科门诊干眼症患者120例(共240只眼),随机分为人工泪液+中药熏眼+雷火灸(A组),人工泪液对照组(B组),人工泪液+中药熏眼对照组(C组),人工泪液+雷火灸对照组(D组),每组各30例(60只眼)。A组予以自拟中药熏蒸双眼,1次/d;雷火灸熏双眼,1次/d;羟丙甲纤维素滴眼液点双眼,4次/d。B组予以羟丙甲纤维素滴眼液点双眼,4次/d。C组予以自拟中药熏蒸双眼,1次/d;羟丙甲纤维素滴眼液点双眼,D组予以雷火灸熏双眼,1次/d;羟丙甲纤维素滴眼液点双眼,4次/d。疗程均为10 d,观察4组患者治疗前后的临床症状评分、泪膜破裂时间(BUT)、泪液分泌实验(Schirmer test I)、角膜荧光素染色(FL),比较4组临床效果。结果:A组治疗有效率为96.7%,B组治疗有效率为60.0%,C组治疗有效率均为80.0%,D组治疗有效率均为83.3%,A组与B、C、D组间比较差异有统计学意义(P<0.05)。A组的临床症状评分、BUT、Schirmer test I评分、FL评分与B、C、D组间比较,差异均有统计学意义(P<0.05)结论:在人工泪液治疗干眼症的基础上结合中药熏眼、雷火灸能够获得较显著的治疗效果。
文摘目的研究中药人工周期联合地屈孕酮片对宫内膜异位症(EMs)不孕患者妊娠及血清学的影响。方法选取2014年3月至2016年3月间在南通市中医院接受治疗的EMs不孕患者96例,依据随机数表法将患者分成两组:对照组(48例)使用地屈孕酮片治疗,观察组(48例)在对照组基础上结合中药治疗;观察患者治疗后妊娠情况、治疗前后血清肿瘤坏死因子-α(TNF-α)、白介素6(IL-6)水平变化情况,使用B超检测患者A型子宫内膜数量,卵泡发育状况,子宫内膜的厚度和排卵日内卵巢血流动力学情况。结果观察组和对照组患者妊娠率分别为54.17%、37.50%,差异有统计学意义(P<0.05);治疗后观察组和对照组患者血清TNF-α[(17.45±4.26)ng/m L vs(22.07±4.36)ng/m L]、IL-6[(18.24±3.16)pg/m L vs(23.17±3.50)pg/m L]、舒张期末流速(EDV)[(11.02±1.43)cm/s vs(9.30±1.86)cm/s]、搏动指数(PI)[(0.69±0.31)vs(1.21±1.05)]、收缩期峰值流速(PSV)[(21.10±1.89)cm/s vs(18.24±1.93)cm/s]比较,差异均有统计学意义(P<0.05)。结论中药人工周期联合地屈孕酮片可改善患者卵泡和子宫内膜的发育与生长,降低炎症因子水平,提高妊娠率。
