A novel asy interacting protein gene (asyip) was isolated from human lung cell line(WI 38) cDNA library with yeast two hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of...A novel asy interacting protein gene (asyip) was isolated from human lung cell line(WI 38) cDNA library with yeast two hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 kb and 2.7 kb in various human tissues at differential level. Putative ASYIP protein contains two large high hydrophobic regions, two protein kinase C phosphorylation sites and two casein kinase Ⅱ phosphorylation sites. A endoplasmic reticulum retrieval signal (Lys Lys Lys Ala Glu) is present at the C terminal of ASYIP protein. Interaction of ASYIP and ASY in mammalian cell was further identified by co immunoprecipitation assay. Like asy gene, overexpression of asyip gene can inhibit growth of tumor cell Saos 2 and induce cell apoptosis, but the efficiency is lower than that of asy gene.展开更多
A novel asy interacting protein gene (asyip) was isolated from human lung cell line(WI-38) cDNA library with yeast two-hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of...A novel asy interacting protein gene (asyip) was isolated from human lung cell line(WI-38) cDNA library with yeast two-hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 kb and 2.7 kb in various human tissues at differential level. Putative ASYIP protein contains two large high hydrophobic regions, two protein kinase C phosphorylation sites and two casein kinase I phosphorylation sites. A endoplasmic reticulum retrieval signal (Lys-Lys-Lys-Ala-Glu) is present at the C-terminal of ASYIP protein. Interaction of ASYIP and ASY in mammalian cell was further identified by co-immunoprecipitation assay. Like asy gene, overexpression of asyip gene can inhibit growth of tumor cell Saos-2 and induce cell apoptosis, but the efficiency is lower than that of asy gene.展开更多
asy gene is a novel apoptosis-inducing gene, but its mechanism is unclear. To investigate the mechanism of asy inducing apoptosis, a novel gene encoding ASY interacting protein (asyip) is isolated from human lung cell...asy gene is a novel apoptosis-inducing gene, but its mechanism is unclear. To investigate the mechanism of asy inducing apoptosis, a novel gene encoding ASY interacting protein (asyip) is isolated from human lung cell line (WI-38) cDNA library with yeast two-hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 and 2.7 kb in various human tissues at different levels. Sequence analysis of full-length cDNA reveals that the two alternative transcripts of asyip gene contain common 5’ end and different 3’ end, and share a common open reading frame encoding a polypeptide of 236 amino acids. Two protein kinase C phosphorylation sites and two casein kinase II phosphorylation sites are found in ASYIP amino acid sequence. Two highly hydrophobic regions encoding potentially two transmembrane domains are present. The ASYIP protein contains a C-terminal endoplasmic reticulum retrieval signal (Lys-Lys-Lys-Ala-Glu). Immunoprecipitation assay confirmed the interaction展开更多
文摘A novel asy interacting protein gene (asyip) was isolated from human lung cell line(WI 38) cDNA library with yeast two hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 kb and 2.7 kb in various human tissues at differential level. Putative ASYIP protein contains two large high hydrophobic regions, two protein kinase C phosphorylation sites and two casein kinase Ⅱ phosphorylation sites. A endoplasmic reticulum retrieval signal (Lys Lys Lys Ala Glu) is present at the C terminal of ASYIP protein. Interaction of ASYIP and ASY in mammalian cell was further identified by co immunoprecipitation assay. Like asy gene, overexpression of asyip gene can inhibit growth of tumor cell Saos 2 and induce cell apoptosis, but the efficiency is lower than that of asy gene.
文摘A novel asy interacting protein gene (asyip) was isolated from human lung cell line(WI-38) cDNA library with yeast two-hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 kb and 2.7 kb in various human tissues at differential level. Putative ASYIP protein contains two large high hydrophobic regions, two protein kinase C phosphorylation sites and two casein kinase I phosphorylation sites. A endoplasmic reticulum retrieval signal (Lys-Lys-Lys-Ala-Glu) is present at the C-terminal of ASYIP protein. Interaction of ASYIP and ASY in mammalian cell was further identified by co-immunoprecipitation assay. Like asy gene, overexpression of asyip gene can inhibit growth of tumor cell Saos-2 and induce cell apoptosis, but the efficiency is lower than that of asy gene.
文摘asy gene is a novel apoptosis-inducing gene, but its mechanism is unclear. To investigate the mechanism of asy inducing apoptosis, a novel gene encoding ASY interacting protein (asyip) is isolated from human lung cell line (WI-38) cDNA library with yeast two-hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 and 2.7 kb in various human tissues at different levels. Sequence analysis of full-length cDNA reveals that the two alternative transcripts of asyip gene contain common 5’ end and different 3’ end, and share a common open reading frame encoding a polypeptide of 236 amino acids. Two protein kinase C phosphorylation sites and two casein kinase II phosphorylation sites are found in ASYIP amino acid sequence. Two highly hydrophobic regions encoding potentially two transmembrane domains are present. The ASYIP protein contains a C-terminal endoplasmic reticulum retrieval signal (Lys-Lys-Lys-Ala-Glu). Immunoprecipitation assay confirmed the interaction
