[Objective] The aim of this study was to investigate spontaneous self- pollination mechanism of Asarum sieboldii. [Methed] Through field observation and manual controlling test under the cultivated condition, the whol...[Objective] The aim of this study was to investigate spontaneous self- pollination mechanism of Asarum sieboldii. [Methed] Through field observation and manual controlling test under the cultivated condition, the whole flowering process of Asarum sieboldii belonging to Asarum subgenus Heterotropa was thoroughly ob- served to investigate its pollination mechanism. [Result] Asarum sieboldii is a kind of species dominated by spontaneous self-pollination. Its female flower became mature firstly, and the duration of single flower ranged from 3 to 4 d. The first day was fe- male phase duration, and the stigmas could receive foreign pollen, while stamen fila- ments bent down towards the base of calyx tube with 90°, and anther had no crack. During the following 2 days, two cycles of stamens erected subsequently, while an- thers became dehiscent and naturally contacted with stigma at the same time, so spontaneous self-pollination was finished. Artificial self-pollination and out-crossing test had proved that the out-crossing of Asarum sieboldii were all fertile with seed setting rate of 100%. Emasculation by bagging test had suggested that Asarum sieboldii had no parthenocarpy and apomixes. [Conclusion] Asarum sieboldii belongs to sub- genus Heterotropa, but its pollination mechanism is similar to that of subgenus Asarum.展开更多
目的:检测细辛药材不同用药部位。肾毒性成分马兜铃酸 A 的含量。方法:高效液相色谱方法,采用 Agilent Zorbax SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-1%冰醋酸水溶液(39:61)为流动相,流速为0.8 mL·min^(-1),检测波长为...目的:检测细辛药材不同用药部位。肾毒性成分马兜铃酸 A 的含量。方法:高效液相色谱方法,采用 Agilent Zorbax SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-1%冰醋酸水溶液(39:61)为流动相,流速为0.8 mL·min^(-1),检测波长为317 nm,分别测定细辛全草和根、芦头、叶柄、叶片、花5个部分马兜铃酸 A 的含量。结果:高效液相色谱方法的回收率为98.7%,马兜铃酸 A 进样量在5.2~68ng 范围内线性关系良好;细辛全草含马兜铃酸 A 16.2μg·g^(-1),根和芦头中含微量马兜铃酸 A,叶柄和叶片中有较多马兜铃酸 A,花中含量最高。结论:细辛地上部分和地下部分马兜铃酸 A 含量差别很大。展开更多
基金Supported by Scientific Research Plan from Shaanxi Provincial Education Department(03jk095)~~
文摘[Objective] The aim of this study was to investigate spontaneous self- pollination mechanism of Asarum sieboldii. [Methed] Through field observation and manual controlling test under the cultivated condition, the whole flowering process of Asarum sieboldii belonging to Asarum subgenus Heterotropa was thoroughly ob- served to investigate its pollination mechanism. [Result] Asarum sieboldii is a kind of species dominated by spontaneous self-pollination. Its female flower became mature firstly, and the duration of single flower ranged from 3 to 4 d. The first day was fe- male phase duration, and the stigmas could receive foreign pollen, while stamen fila- ments bent down towards the base of calyx tube with 90°, and anther had no crack. During the following 2 days, two cycles of stamens erected subsequently, while an- thers became dehiscent and naturally contacted with stigma at the same time, so spontaneous self-pollination was finished. Artificial self-pollination and out-crossing test had proved that the out-crossing of Asarum sieboldii were all fertile with seed setting rate of 100%. Emasculation by bagging test had suggested that Asarum sieboldii had no parthenocarpy and apomixes. [Conclusion] Asarum sieboldii belongs to sub- genus Heterotropa, but its pollination mechanism is similar to that of subgenus Asarum.
文摘目的:检测细辛药材不同用药部位。肾毒性成分马兜铃酸 A 的含量。方法:高效液相色谱方法,采用 Agilent Zorbax SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈-1%冰醋酸水溶液(39:61)为流动相,流速为0.8 mL·min^(-1),检测波长为317 nm,分别测定细辛全草和根、芦头、叶柄、叶片、花5个部分马兜铃酸 A 的含量。结果:高效液相色谱方法的回收率为98.7%,马兜铃酸 A 进样量在5.2~68ng 范围内线性关系良好;细辛全草含马兜铃酸 A 16.2μg·g^(-1),根和芦头中含微量马兜铃酸 A,叶柄和叶片中有较多马兜铃酸 A,花中含量最高。结论:细辛地上部分和地下部分马兜铃酸 A 含量差别很大。