Background: The objective of this study was to determine the levels of tumor necrosis factor-alpha (TNF-α) and receptor activator of NF-kB ligand (RANKL) in the gingival crevicular fluid (GCF) in patients with severe...Background: The objective of this study was to determine the levels of tumor necrosis factor-alpha (TNF-α) and receptor activator of NF-kB ligand (RANKL) in the gingival crevicular fluid (GCF) in patients with severe root resorption after orthodontic treatment. Materials and Methods: Ten patients who had been receiving orthodontic treatment (5-control subjects and 5-severe root resorption subjects) participated in this study. GCF was collected from all patients. Subjects with severe root resorption (>1/3 of the original root length) were identified. Control group subjects with no loss of the root structure undergoing orthodontic treatment were also identified. The GCF was collected non-invasively from the mesial and distal sides of each of the upper central and lateral incisors using filter paper strips. The eluted GCF was used for a Western blot analysis with Antibodies against TNF-α and soluble RANKL (sRANKL). Ten male 6-week-old Wistar rats were subjected to orthodontic force of 50 g to induce a mesially tipping movement of the upper first molars for 7 days. The expression levels of TNF-α and RANKL proteins were determined in periodontal ligament (PDL) by immunohistochemical analysis. Results: The Western blot analysis showed that the TNF-α and sRANKL expressions were significantly higher in the severe root resorption group than in the control group. In the experimental tooth movement in vivo, resorption lacunae with multinucleated cells were observed in 50 g group. The immunoreactivity for TNF-α and RANKL was detected in PDL tissue subjected to the orthodontic force on day 7. Conclusion: These results suggest that TNF-α and RANKL play important roles in inducing or facilitating the development of orthodontically-induced inflammatory root resorption (OIIRR).展开更多
目的:研究快速扩弓前后龈沟液天冬氨酸转氨酶(AST)与碱性磷酸酶(ALP)水平的变化,以及其与快速扩弓牙周组织改建的关系。方法:选择38例快速扩弓的患者(10．5-12．8岁),随机分为对照组(18例)和加力组(20例)。利用全自动化生化分析仪检测...目的:研究快速扩弓前后龈沟液天冬氨酸转氨酶(AST)与碱性磷酸酶(ALP)水平的变化,以及其与快速扩弓牙周组织改建的关系。方法:选择38例快速扩弓的患者(10．5-12．8岁),随机分为对照组(18例)和加力组(20例)。利用全自动化生化分析仪检测快速扩弓前,快速扩弓24h、7d,及保持7d、14d、28d的龈沟液AST、ALP水平的变化,结果以酶总量／牙表示。采用SAS(r)Proprietary Software Version 9．00对数据进行配对t和两样本t检验。结果:加力组AST水平在快速扩弓24h后开始升高(P<0．05),对照组在快速扩弓7d后开始升高(P<0．01),2组AST水平至保持28d时一直维持在较高水平(P<0．01);扩弓24h至保持28d,加力组与对照组AST水平存在显著差异(P<0．05)。加力组和对照组ALP水平在快速扩弓7d后开始升高,至保持28d时一直维持在较高水平(P<0．01);从扩弓7d至保持28d,加力组与对照组ALP水平存在显著差异(P<0．01)。结论:龈沟液AST、ALP水平可以在一定程度上反映快速扩弓后牙周组织的改建。展开更多
文摘Background: The objective of this study was to determine the levels of tumor necrosis factor-alpha (TNF-α) and receptor activator of NF-kB ligand (RANKL) in the gingival crevicular fluid (GCF) in patients with severe root resorption after orthodontic treatment. Materials and Methods: Ten patients who had been receiving orthodontic treatment (5-control subjects and 5-severe root resorption subjects) participated in this study. GCF was collected from all patients. Subjects with severe root resorption (>1/3 of the original root length) were identified. Control group subjects with no loss of the root structure undergoing orthodontic treatment were also identified. The GCF was collected non-invasively from the mesial and distal sides of each of the upper central and lateral incisors using filter paper strips. The eluted GCF was used for a Western blot analysis with Antibodies against TNF-α and soluble RANKL (sRANKL). Ten male 6-week-old Wistar rats were subjected to orthodontic force of 50 g to induce a mesially tipping movement of the upper first molars for 7 days. The expression levels of TNF-α and RANKL proteins were determined in periodontal ligament (PDL) by immunohistochemical analysis. Results: The Western blot analysis showed that the TNF-α and sRANKL expressions were significantly higher in the severe root resorption group than in the control group. In the experimental tooth movement in vivo, resorption lacunae with multinucleated cells were observed in 50 g group. The immunoreactivity for TNF-α and RANKL was detected in PDL tissue subjected to the orthodontic force on day 7. Conclusion: These results suggest that TNF-α and RANKL play important roles in inducing or facilitating the development of orthodontically-induced inflammatory root resorption (OIIRR).
文摘目的:研究快速扩弓前后龈沟液天冬氨酸转氨酶(AST)与碱性磷酸酶(ALP)水平的变化,以及其与快速扩弓牙周组织改建的关系。方法:选择38例快速扩弓的患者(10．5-12．8岁),随机分为对照组(18例)和加力组(20例)。利用全自动化生化分析仪检测快速扩弓前,快速扩弓24h、7d,及保持7d、14d、28d的龈沟液AST、ALP水平的变化,结果以酶总量／牙表示。采用SAS(r)Proprietary Software Version 9．00对数据进行配对t和两样本t检验。结果:加力组AST水平在快速扩弓24h后开始升高(P<0．05),对照组在快速扩弓7d后开始升高(P<0．01),2组AST水平至保持28d时一直维持在较高水平(P<0．01);扩弓24h至保持28d,加力组与对照组AST水平存在显著差异(P<0．05)。加力组和对照组ALP水平在快速扩弓7d后开始升高,至保持28d时一直维持在较高水平(P<0．01);从扩弓7d至保持28d,加力组与对照组ALP水平存在显著差异(P<0．01)。结论:龈沟液AST、ALP水平可以在一定程度上反映快速扩弓后牙周组织的改建。
