Five new racemic N-acetyldopamine (NADA) trimers, asponchimides A−E ( 1 − 5 ), were isolated from Aspongopus chinensis, a prominent traditional Chinese medicinal insect employed for alleviating pain, treating indigest...Five new racemic N-acetyldopamine (NADA) trimers, asponchimides A−E ( 1 − 5 ), were isolated from Aspongopus chinensis, a prominent traditional Chinese medicinal insect employed for alleviating pain, treating indigestion, and addressing kidney ailments. Compounds 1 − 5 were successfully resolved by chiral high-performance liquid chromatography (HPLC), yielding five pairs of enantiomers: (+)- and (−)-asponchimides A−E ( 1a / 1b − 5a / 5b ). Their structural identities were discerned by extensive spectroscopic analyses, including high-resolution mass spectrometry (HRMS), ultraviolet-visible (UV-Vis) spectroscopy, infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR), and their absolute configurations were determined by electronic circular dichroism (ECD) calculations. Compounds 1 − 5 are pioneering instances of NADA trimers featuring a Δ7 double bond. When subjected to a series of bioassays, a majority of the compounds exhibited weak inhibitory activity against nitric oxide (NO) production in LPS-induced RAW 264.7 cells.展开更多
OBJECTIVE:To investigate the effects of Aspongopus(A.)chinensis hemolymph on the proliferation and metastasis of breast cancer cells.METHODS:The in vitro effects of A.chinensis hemolymph were investigated in murine(4T...OBJECTIVE:To investigate the effects of Aspongopus(A.)chinensis hemolymph on the proliferation and metastasis of breast cancer cells.METHODS:The in vitro effects of A.chinensis hemolymph were investigated in murine(4T1)and human(HCC1937)breast cancer cell lines.Cytotoxicity,cell apoptosis,and cell migration were evaluated by using the cell counting kit-8 assay,Hoechst staining,and wound healing experiments,respectively.A syngeneic mouse model was established to evaluate the in vivo effects of the hemolymph extract on tumor growth and metastasis.Mouse body weight,tumor size,blood levels of function-related enzymes,and pathological features of the liver and kidney tissues were evaluated.RESULTS:The hemolymph of A.chinensis significantly inhibited in vitro tumor cell migration and viability while inducing apoptosis.Furthermore,it inhibited in vivo tumor growth and metastasis with a minimal effect on mouse body weight,and did not induce liver or kidney damage.CONCLUSION:Our results suggested that the A.chinensis hemolymph has antitumorigenic properties,suggesting it has potential as a novel therapeutic option for the treatment of metastatic breast cancer.展开更多
基金supported by the Key Program of National Natural Science Foundation of China(NO.82130111)High-level Innovative Research Institute of Department of Science and Technology of Guangdong Province(No.2021B0909050003)High-level New R&D Institute of Department of Science and Technology of Guangdong Province(No.2019B090904008)。
文摘Five new racemic N-acetyldopamine (NADA) trimers, asponchimides A−E ( 1 − 5 ), were isolated from Aspongopus chinensis, a prominent traditional Chinese medicinal insect employed for alleviating pain, treating indigestion, and addressing kidney ailments. Compounds 1 − 5 were successfully resolved by chiral high-performance liquid chromatography (HPLC), yielding five pairs of enantiomers: (+)- and (−)-asponchimides A−E ( 1a / 1b − 5a / 5b ). Their structural identities were discerned by extensive spectroscopic analyses, including high-resolution mass spectrometry (HRMS), ultraviolet-visible (UV-Vis) spectroscopy, infrared (IR) spectroscopy, and nuclear magnetic resonance (NMR), and their absolute configurations were determined by electronic circular dichroism (ECD) calculations. Compounds 1 − 5 are pioneering instances of NADA trimers featuring a Δ7 double bond. When subjected to a series of bioassays, a majority of the compounds exhibited weak inhibitory activity against nitric oxide (NO) production in LPS-induced RAW 264.7 cells.
基金Supported by grants from the National Nature Science Foundation of China:Isolation and Identification of Anti-gastric-cancer components from Aspongopus chinensis Dallas with its Mechanism(NSFC 81360612)Inhibitory Effect of lentivirus-mediated Cyt-c Gene from Aspongopus chinensis Dallas on Breast Cancer Cell in vitro and in vivo(NSFC81803968)Graduate Education Innovation Project of Guizhou Province:Virus-like Particles Mediated FGF1-P2A-CYCS Fusion Protein induced Transmembrane Transport and Target Cancer Cells Apoptosis(Qian Jiao He YJSCXJH,No.(2019)025)。
文摘OBJECTIVE:To investigate the effects of Aspongopus(A.)chinensis hemolymph on the proliferation and metastasis of breast cancer cells.METHODS:The in vitro effects of A.chinensis hemolymph were investigated in murine(4T1)and human(HCC1937)breast cancer cell lines.Cytotoxicity,cell apoptosis,and cell migration were evaluated by using the cell counting kit-8 assay,Hoechst staining,and wound healing experiments,respectively.A syngeneic mouse model was established to evaluate the in vivo effects of the hemolymph extract on tumor growth and metastasis.Mouse body weight,tumor size,blood levels of function-related enzymes,and pathological features of the liver and kidney tissues were evaluated.RESULTS:The hemolymph of A.chinensis significantly inhibited in vitro tumor cell migration and viability while inducing apoptosis.Furthermore,it inhibited in vivo tumor growth and metastasis with a minimal effect on mouse body weight,and did not induce liver or kidney damage.CONCLUSION:Our results suggested that the A.chinensis hemolymph has antitumorigenic properties,suggesting it has potential as a novel therapeutic option for the treatment of metastatic breast cancer.
