β-胡萝卜合成途径的不同启动子组合的比较研究

以耶氏解脂酵母菌(Yarrowialipolytica)MYA2613为底盘菌,表达来源于卷枝毛霉菌(Mucorcircinelloides)的基因car B,carRP后能有效生产β-胡萝卜素。启动子作为调控基因的重要顺式元件影响着基因的表达。为了优化基因GGS1,car B,carRP的表达强度组合,将上述3个基因、终止子与两组不同的启动子通过DNA assemble方法拼接后,得到了组合TEF1p-GGS1-xpr2t-GPD1pcar B-mig1t-EXP1p-carRP-lip2t和YAT1p-GGS1-xpr2t-GPD1p-car B-mig1t-FBAin1p-carRP-lip2t。经发酵表型验证,两株菌都产β-胡萝卜素,其中前者的产量是后者的4.18倍,具有明显优势。

Adjustable Luminescence of SrAl_2O_4:Eu^(2+),Dy ^(3+) Assembled in Zeolite

Capsulating guest into the nanometer voids of zeolites is a effective way to form novel host-guest material. In our work, stoichiometric SrAl2O4 : Eu2+, Dy3+ sol guest was prepared by sol-gel method and assembled into the nanometer channels of zeolite ZSM-5 host through mechanical mixing, hydrothemial reaction and microwave heating reaction, respectively. After being reduced and diffused in a microwave muffle, the fluorescence spectra of the host-guest materials exhibit remarkable blue shifts in companison of that of SrAl2O4 : Eu2+, Dy3+ Some interesting phenomena in the assembled host-guest materials are that the after-low emission spectra exist two bands at about 400 nm and 517 nm and the relative strengths of these two hands can be adjusted by changing the assembly methods and the assembly concentration. These are attributed to the fact that the phosphor was capsuiated into the voids of zeolite ZSM-5 and generated the quantum size effect and the host-guest effect.

Surface-enhanced Raman scattering properties of highly ordered self-assemblies of gold nanorods with different aspect ratios

Gold nanorods with aspect ratios of from 1 （particles） to 31.6 were synthesized by the seed-mediated method and packed in a highly ordered structure on a large scale on silicon substrates through capillary force induced self-assembly behaviour during solvent evaporation. The gold nanorod surface exhibits a strong enhancing effect on Raman scattering spectroscopy. The enhancement of Raman scattering for two model molecules （2-naphthalenethiol and rhodamine 6C） is about 5-6 orders of magnitude. By changing the aspect ratio of the Au nanorods, we found that the enhancement factors decreased with the increase of aspect ratios. The observed Raman scattering enhancement is strong and should be ascribed to the surface plasmon coupling between closely packed nanorods, which may result in huge local electromagnetic field enhancements in those confined junctions.

STUDY ON THE COMPLICATED DYNAMICAL BEHAVIORS OF NONLINEAR ECOSYSTEMS(Ⅲ)

This paper is a continuous study of references[1-2].At first,we introduce the concept of assembles of ecosystems,and then discuss macro-and micro-synergetical methods for ecology study.Combining the two methods,by use of the macroscopic data(observable)outputted from the ecosystems,we can construct their GGLE,master their information changes between,before and after the generalized phase changes(e.g.community successions),and find out the action mechanisms of microscopic factors on macroscopic results.This may be a new approach to the study of action mechanisms of complex ecosystems.

Self-assembled interlayer aiming at the stability of NiOx based perovskite solar cells

Inorganic NiO_(x) based inverted structure perovskite solar cells (PSCs) is reported to be more stable than that with the organic hole transport materials.In this work,NiO_(x)/MAPbI_(3) interface chemical reaction induced instability of perovskite is unveiled:Ni^(3+) and I^(-) exhibit redox reactions and deprotonation of MA^(+) happens,which result in interface defects and perovskite lattice deformation.Thus the defective interface accelerates the degradation of perovskite by defect pathways from the bottom interface to the perovskite surface contacting H_(2)O/O_(2).Self-assembled interlayer of NH_(2)^(-)end silane on NiO_(x)separates the reactive NiO_(x)and MAPbI_(3),tunes the interface energy states by–NH_(2) end group.As a result,the PSC based on the silane treated NiO_(x)achieves enhanced PCE of 20.1%with decent stability under environmental and extreme conditions (high temperature,high humidity,light infiltration).Our work highlights the interface chemical problem induced PSC instability and a simple interface modification to achieve the stable PSCs.

Finite Element Analysis of the 2 m Telescope Assemble

To improve the performance of the 2 m telescope, the optimum design is applied to the telescope assemble. Referring to the telescope assemble with the dimetric truss, a group of reasonable sizes of the telescope assemble are found by optimization methods and modal analysis, which will raise the resonant frequency by 4.21 %. As a result, the telescope assemble is less likely to resonate. Besides, the dynamic response module in ANSYS is utilized to analyze the modal type, harmonic vibration response and random vibration response of the telescope assemble. By the calculation of ANSYS, finite dement analysis （FEA） method proves that the performance of the telescope assemble is mildly enhanced by means of optimum design.

Seal Structure of Vacuum System for CSR

To reach the design parameter of CSR, vacuum system (≤3.5x10^(-9) Pa), besides the high requirement for quality of the material and vacuum sanitation of the vacuum cells, the seal function is the most important. All cells of vacuum system for CSR are limited by the situations and rooms of gap of magnets, radio frequency system, diagnostic devices and others, and must meet the assemble and disassemble requirements of every devices.

Software Design of Multi-step Temperature Controller

The generic temperature controller can keep the temperature in one zone, but in our practical industry control we need keep the temperature at several different zones, and at each temperature zone we need keep different length of time. So we designed this system. The multi-step temperature controller is based on AT89C51, and programmed with assemble language (MCS-51 repertoire).

说文解字话英语

二十五九九归一(sem-) 英语单词same和some都能追溯到同一个印欧语词根sem-(单一,同一个),用英语表示的意思是one或the same。由拉丁语通过法语进入英语的semblance(相似,外貌)就是一个显而易见的例子,所谓“相似”也就是有同样的或类似的外貌。加上表示否定的前缀dis-而构成的dissemble(掩饰,假装),dis-semblance,dissembler无非是“装出不是跟某种外貌同样子”而已。我们还可以列举出其它的单词,如resemble(类似,相似),assemble(收集,装配),ensemble(全体,整套服装)等。印欧语词根sem-的变体sim-在英语单词中出现得更多。similar(相似的)就是最常见的一个。

风格探索之旅 上海Assemble by Réel概念店

Kokaistudios从上海千禧一代的多元化生活方式中汲取设计灵感,真正将自然引入了Assemble by Réel概念店,带领顾客进行一场风格之旅。清晰的平面布局配以抓人眼球的艺术装置,设计引导顾客穿行于特色鲜明的空间,令人印象深刻。Assemble by Réel位于上海高奢购物中心芮欧百货的三层,是一家男士时尚及生活方式概念店。

主张时尚的商务魅力——Assemble esclave 连奴的新都市女性

如果，你还记得板的穿着对小开襟的小毛衫，黑色一字裙一板一眼的过着两点一线的生活，那么你已经远远的被那些新都市女性抛在后面了。那些在休闲与商务间完美的变幻角色的高手，每一刻都焕发出宜人的魅力。

ATI: Assemble topological interaction overcomes consistency– cohesion trade‐off in bird flocking

In nature,various animal groups like bird flocks display proficient collective navigation achieved by maintaining high consistency and cohesion simultaneously.Both metric and topological interactions have been explored to ensure high consistency among groups.The topological interactions found in bird flocks are more cohesive than metric in-teractions against external perturbations,especially the spatially balanced topological interaction(SBTI).However,it is revealed that in complex environments,pursuing cohesion via existing interactions compromises consistency.The authors introduce an innovative solution,assemble topological interaction,to address this challenge.Con-trasting with static interaction rules,the new interaction empowers individuals with self-awareness to adapt to the complex environment by switching between interactions through visual cues.Most individuals employ high-consistency k-nearest topological interaction when not facing splitting threats.In the presence of such threats,some switch to the high-cohesion SBTI to avert splitting.The assemble topological interaction thus transcends the limit of the trade-off between consistency and cohesion.In addition,by comparing groups with varying degrees of these two features,the authors demonstrate that group effects are vital for efficient navigation led by a minority of informed agents.Finally,the real-world drone-swarm experiments validate the applicability of the proposed interaction to artificial robotic collectives.

Photoisomerization,assembling and fluorescence photoswitching behaviors of a water-soluble stiff-stilbene with cucurbit[7]uril

Photoresponsive supramolecular systems have merited extensive attention for their applications in materials science and life science.Here,we synthesized a water-soluble stiff-stilbene molecular photoswitch,exhibiting outstanding photoisomerization reaction between its(E)-and(Z)-configurations upon irradiation at distinct light.The photoswitch can assemble with cucurbit[7]uril(CB[7])to form a superior fluorescent supramolecular complex(compared to the only guest)with excellent water solubility,which manifested dramatic photoluminescence enhancement caused by restriction of intramolecular rotation and remained good photochromic characteristics.Furthermore,introduction of CB[7]influence photoreaction quantum yield(Φ)of the stiff-stilbene,leading to reduction ofΦ_(E→Z) and increase ofΦ_(Z→E) of the stiff-stilbene.Importantly,the photoadjustable supramolecular assembly can act as a fluorescence switch,and the phototunable guest further selectively modulate G-quadruplex structure of Tel22 upon light irradiation or with addition of CB[7].The study provides a new simple way for accurately regulating photochromic properties of molecular switches and developing smart materials with potential applications in controlled modulation of G-quadruplex,targeted biological imaging and so on.

Critical role of bioinformatics in translating huge amounts of next-generation sequencing data into personalized medicine

Realizing personalized medicine requires integrating diverse data types with bioinformatics.The most vital data are genomic information for individuals that are from advanced next-generation sequencing(NGS) technologies at present.The technologies continue to advance in terms of both decreasing cost and sequencing speed with concomitant increase in the amount and complexity of the data.The prodigious data together with the requisite computational pipelines for data analysis and interpretation are stressors to IT infrastructure and the scientists conducting the work alike.Bioinformatics is increasingly becoming the rate-limiting step with numerous challenges to be overcome for translating NGS data for personalized medicine.We review some key bioinformatics tasks,issues,and challenges in contexts of IT requirements,data quality,analysis tools and pipelines,and validation of biomarkers.

Assembly and features of secondary metabolite biosynthetic gene clusters in Streptomyces ansochromogenes

A draft genome sequence of Streptomyces ansochromogenes 7100 was generated using 454 sequencing technology. In combination with local BLAST searches and gap filling techniques, a comprehensive antiSMASH-based method was adopted to assemble the secondary metabolite biosynthetic gene clusters in the draft genome of S. ansochromogenes. A total of at least 35 putative gene clusters were identified and assembled. Transcriptional analysis showed that 20 of the 35 gene clusters were expressed in either or all of the three different media tested, whereas the other 15 gene clusters were silent in all three different media. This study provides a comprehensive method to identify and assemble secondary metabolite biosynthetic gene clusters in draft genomes of Streptomyces, and will significantly promote functional studies of these secondary metabolite biosynthetic gene clusters.

pH-Sensitive assembly/disassembly gold nanoparticles with the potential of tumor diagnosis and treatment

Due to the ability to combine the separately unique characteristics of assembled and disassembled nanoparticles(NPs), the stimuli-responsive self-assembly of NPs has attracted considerable interest in functional material applications especially biomaterials. Here we demonstrate a facile and versatile approach to regulate the self-assembly process and transition pH of Au NPs by fine-tuning the co-modified pH-responsive compounds and poly(ethylene glycol)(PEG). Importantly the transition pH(ΔpH=0.4) of the system can be predetermined in the range of 8.2–5.8(assembled to disassembled) and 8.2–4.2(disassembled to assembled), which ideally covers the pH of normal tissue, tumor tissue milieu and organelles. The results of fluorescence imaging, Raman spectroscopy and photothermal conversion of the stimuli-responsive Au NPs shows the potential application for tumor specificity theranostics. In a nutshell this study provides a useful toolkit to design tumor-activatable self-assembled NPs with high specificity and universality.

Rapid Shape Memory and p H-modulated Spontaneous Actuation of Dopamine Containing Hydrogels

The dopamine containing hydrogels with rapid responsive shape memory capability were synthesized by a one-pot method. The temporary shape of hydrogel was fixed within 20 s in Na OH solution by the tris-complex crosslinking of metalligand complex between Fe3＋ ions and catechol groups, while the permanent shape was recovered completely in HCl solution within 60 s upon the change from tris-complex to mono-complex. The hydrogel showed unique spontaneous actuation behavior. It could curl spontaneously without further external force deformation when immersed in Na OH solution again after the first shape recovery in HCl solution. This might be attributed to the competitive result of swelling and additional tris-complex crosslinking formation when immersed in Na OH solution. In addition, the hydrogels also had proper modulus, elongation ratio and tensile strength. Such hydrogel provides a new candidate material for designing soft actuators and robots modulated with spontaneous actuating.

Growth and micromagnetic simulation of magnetite nanoparticles

Magnetite nanoparticles with different sizes and different assemblies were synthesized via hydrothermal method.Micromagnetic simulation shows the magnetite nanocubes with different sizes have different energy states,which determines the assembly mode.Magnetite nanocubes with the side length of 30-60 nm tended to be dispersed while both nanochains and dispersed nanoparticles were found to grow for the nanocubes with the side length less than 30 nm,which can be explained in the fact that the above two assembly modes have very close energies.The assembly mode of nanocubes with the size bigger than 60 nm is generally nanorings consisting of connected inter-grown nanocubes and the nanocubes are no longer single domain.The simulations are consistent with the experimental results.

尽享上海生活方式之旅——Assemble by Réel

Kokaistudios一家涵盖建筑及室内设计的事务所,在业界屡获殊荣,由意大利建筑师Filippo Gabbiani和Andrea Destefanis于2000年联合创立于威尼斯。自2002年公司总部设于上海以来,Kokaistudios已拥有60余位杰出设计师,并成长为一家多元文化背景的设计公司,与客户协同合作,完成了涵盖亚洲、中东、欧洲及北美洲的众多创新设计项目。

Copper-sulfide cluster assembled architecture via in situ reaction

A new copper-thiolate cluster assembled framework[Cu2(μ4-SCH3)Cl]n(1),has been solvothermally synthesized through in situ reaction viz.,in situ ligand generation and metal reduction.Compound 1 represents the first 3 D framework based on Atlas-sphere functionalized by singleμ2-Cl groups.DOS calculation reveals the interaction of electronic structures.It is found that the HOMO is mainly distributed on Cl,Cu and S bonding orbitals,while the LUMO is dominated by Cu-Cl antibonding orbitals.