Optimization of acidic extraction of astaxanthin from Phaffia rhodozyma

Optimization of a process for extracting astaxanthin from Phaffia rhodozyma by acidic method was investigated,regarding several extraction factors such as acids,organic solvents,temperature and time. Fractional factorial design,central composite design and response surface methodology were used to derive a statistically optimal model,which corresponded to the following optimal condition: concentration of lactic acid at 5.55 mol/L,ratio of ethanol to yeast dry weight at 20.25 ml/g,tem-perature for cell-disruption at 30 °C,and extraction time for 3 min. Under this condition,astaxanthin and the total carotenoids could be extracted in amounts of 1294.7 μg/g and 1516.0 μg/g,respectively. This acidic method has advantages such as high extraction efficiency,low chemical toxicity and no special requirement of instruments. Therefore,it might be a more feasible and practical method for industrial practice.

Efficient Extraction of Astaxanthin from Phaffia rhodozyma with Polar and Non-polar Solvents after Acid Washing

在酸的洗以后与各种各样的溶剂从 Phaffia rhodozyma 提取 astaxanthin 的方法被调查。抽取效率清楚地在 P 的酸的洗以后被增加。rhodozyma 房间。当 HCl 的集中是 0.4 摩尔时 ?

法夫酵母虾青素提取工艺的优化研究

从破壁方法、浸提溶剂及提取条件等方面对法夫酵母虾青素提取工艺进行了优化研究。用单因子试验对破壁方法及浸提溶剂进行选择,结果表明二甲亚砜(DMSO)法是法夫酵母破壁提取虾青素的最佳方法,丙酮是理想的提取溶剂。用析因试验对破壁提取的条件进行了分析研究,结果表明破壁的温度、破壁的时间、浸提溶剂的添加量及浸提温度等都会对法夫酵母破壁提取虾青素产生显著影响,但以破壁的温度及丙酮的添加量影响最大。通过最速上升和中心组合设计试验,优化得到适宜的提取条件为:二甲亚砜加量25mL/g(以干菌体计,下同)、破壁温度75.6℃、破壁时间20min、丙酮添加量77.4mL/g、浸提温度为40℃,优化后提取液中虾青素的浓度为3.145μg/mL,比未优化时增加了27.02%。

高温湿热酸法破壁提取法夫酵母胞内虾青素

法夫酵母是一种能积累虾青素的红酵母,对其进行破壁是当前虾青素工业化提取生产的瓶颈工艺。研究在高温湿热条件下,低浓度盐酸对法夫酵母破壁而提取其胞内虾青素的工艺。探讨了不同破壁温度、盐酸浓度、液料比与破壁处理时间等因素对法夫酵母破壁后虾青素及类胡萝卜素提取率的影响,确定了高温湿热酸法破壁提取虾青素的最佳条件为:饱和蒸汽压力0.1MPa(121°C),盐酸浓度0.5mol/L,液料比(V/W)30mL/g,破壁时间2min。在最佳条件下进行中试放大实验,可得到虾青素与类胡萝卜素的提取率分别为:(84.8±3.2)%,(93.3±2)%。经优化后的新破壁工艺安全高效,不会有毒性残留,具有良好的工业应用前景。