Effect of Acupoint Liquid Nitrogen Cryotherapy on Neuro-Endocrine-Immune Network System in Patients with Lung Qi Deficiency Syndrome in Remission Stage of Bronchial Asthma

[Objectives]To observe the effect of acupoint liquid nitrogen cryotherapy on the neuro-endocrine-immune network system of lung qi deficiency syndrome during the remission period of bronchial asthma and explore its possible mechanism.[Methods]A total of 100 patients with bronchial asthma were randomly divided into control group and observation group,with 50 cases in each group.The control group was given budesonide spray inhalation treatment,combined with acupoint liquid nitrogen freezing treatment,twice a year for a course of treatment,followed up for 1 year.The other 50 healthy volunteers were included in the healthy group,and no treatment was given in the healthy group.Serum immunoglobulins A,G,E(IgA,IgG,IgE)were detected before and after treatment in each group,serum cytokines[interleukin-4(IL-4),interleukin-10(IL-10),tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ)],plasma neurotransmitters[substance P(SP),vasoactive intestinal peptide(VIP)].[Results]Before treatment,compared with healthy group,the contents of IgA,IgG,IL-10,IFN-γand VIP in observation group and control group were decreased,while the contents of IgE,IL-4,TNF-αand SP were increased,with statistical significance(P<0.05).After treatment,compared with before treatment,IgA,IgG,IL-10,IFN-γand VIP contents in observation group and control group were increased(P<0.05),and observation group was higher than control group(P<0.05),IgE,IL-4 and SP contents in observation group and control group were decreased(P<0.05).The observation group was lower than the control group(P<0.05).[Conclusions]Acupoint liquid nitrogen cryotherapy may improve the immune function of the body,regulate the release of cytokines and regulate neuromediators,and thus play a role in the prevention and treatment of bronchial asthma.

OBSERVATION ON THE THERAPEUTIC EFFECT OF ACUPOINT APPLICATION OF FAR-INFRARED ASTHMA-RELIEVING PLASTER FOR ASTHMA

Objective To observe the clinical effect of acupoint application of Yuanhongwai Xiaochuankang Plaster （Far-infrared Asthma-relieving Plaster, FIRARP） for prevention and treatment of asthma. Methods Three hundred and sixty-six cases of bronchial asthma were randomly divided into treatment group （n = 185） and control group （n = 181 ）. Patients of treatment group were treated with FIRARP and those of control group treated with external application of traditional ＂Sonfu （Dog-days） moxibustion＂ （herbal-paste-cake separated moxibustion） and acupoints used were Dazhui（大椎 GV14）, Feishu（肺俞BL13）, Tiantu （天突 CV22）, etc. which were given to the patients. Forced expiratory volume in 1 second （FEV1） and peak expiratory flow （PEF） of the pulmonary function were determined and the changes of main symptoms and signs were observed before and after the treatment. Results The pulmonary functions were effectively improved, the symptoms were controlled, and the asthma attack was reduced in the two groups with no significant difference between the two groups （P 〉0.05）. Conclusion There is no significant difference between Yuanhongwai Xiaochuonkong Plaster and traditional ＂Sanfu moxibustion＂ on bronchial asthma. It may substitute for traditional ＂Sonfu moxibustion＂.

CLINICAL OBSERVATION ON TREATMENT OF ALLERGIC BRONCHIAL ASTHMA WITH ACUPOINT APPLICATION THERAPY

In the present paper, the therapeutic effect of acupoint application of Chinese Materia Medica in the treatment of allergic asthma was observed. The results revealed that topical application of drug paste [mixture of powder of Mahuang (Herba Ephedrae), Xixin (Herba Asari), Baijiezi (Semen Sinapis Albae), etc.] at the acupuncture points could improve symptoms and signs of allergic asthma patients, with the total effective rate being 69.57% (16/23) for the short term effect, and 52.17%(12/23) for the long term effect. In medication (Aminophylline) group, of the 25 cases of allergic asthma, 16 (64.0%) were effective after 4 weeks of treatment, while 6 (24.0%) were still effective that was shown by 6 months’ follow up. The therapeutic effect of acupoint application group was significantly better than that of medication group in the long term effect. Results also showed that no obvious interrelation between the short term therapeutic effect and the duration or the severity of the disease. Results also display that acupoint application therapy is an effective, safe and handy method with fewer side effects for asthma.

ANALYSIS OF CURATIVE EFFECTS OF BRONCHITIS AND ASTHMA TREATED BY CATGUT IMPLANTATION AT ACUPOINT

Catgut implantation at acupoint, catgut implantation at acupoint plus medicine and simple medicine were respectively used for treatment of 100 cases with bronchitis and asthma.The results showed that the effective rates of catgut implantation at acupoint, catgut implantation atacupoint plus medicine and simple medicine were 87%, 99% and 85% respectively. Statistical analy-sis indicated that the curative effect of catgut implantation plus medicine was the best as comparedwith other two groups with significant difference (P【0.01 ).

Prevention and Treatment of 88 cases of Asthma with Acupoint Application

Prevention and treatment of 88 cases of asthma with acupoint application therapy.Analyses of the mechanism of treatment according to the Traditional Chinese Medicine(TCM)theory and laboratory test results.

Acupoint injection for asthma:Systematic review of randomized controlled trials

Objective:To assess the effectiveness and safety of acupoint injection for the treatment of asthma.Methods:Six electronic databases were searched for randomized controlled trials(RCTs)of acupoint injection for asthma.Two authors extracted data and assessed methodological quality independently using the Cochrane Collaboration Risk of Bias tool.Data were evaluated using RevMan v5.2.Results:Eighteen RCTs involving 1913 participants with asthma were identified.Overall methodological quality of the RCTs was classified as unclear risk of bias.Western medicine(12 RCTs)was injected most frequently into acupoints,followed by Chinese herbal medicine(four RCTs),vitamins(one RCT),and Chinese herbal medicine combined with Western medicine(one RCT).Four RCTs used only one acupoint[ST36(two RCTs),BL13,CV22],whereas the other RCTs selected multiple acupoints(among which BL13 was used most frequently).One RCT reported mortality,no RCT reported quality of life,15 RCTs reported the symptom improvement rate,one RCT reported asthma control test(ACT)data,one RCT reported the duration of asthma,three RCTs reported the mean time that asthma was controlled(MTAC),and 13 RCTs reported lung-function tests(LFTs).Some RCTs showed acupoint injection may improve the attack time of asthma,MTAC,and LFTs.Five RCTs reported the outcome of adverse events and showed no significant differences between the acupoint injection group and control group.Conclusions:The findings suggest that acupoint injection may be effective for improving ACT data,duration of asthma,MTAC and LFTs.However,the evidence is insufficient owing to the poor methodological quality of the RCTs.

Clinical Study of Acupoint Application of Jiezi Fangxiao Ointment Combined with Pingchuan Prescription in the Treatment of Pediatric Asthma

[Objectives]To study the clinical effect of Acupoint Application of Jiezi Fangxiao Ointment combined with Pingchuan Prescription in the treatment of pediatric asthma.[Methods]A total of 40 children with asthma admitted to the First Affiliated Hospital of Heilongjiang University of Chinese Medicine from July 2017 to September 2018 were selected as the research subjects,and divided into control group and observation group according to random classification method,with 20 cases in each group.Among which the control group was given routine symptomatic treatment,the observation group was treated with Acupoint Application of Jiezi Fangxiao Ointment combined with Pingchuan Prescription on the basis of the control group.After 4 weeks of continuous treatment,the clinical efficacy was observed and the changes of traditional Chinese medicine symptom scores,forced expiratory volume in the first second(FEV1),maximum expiratory flow(PEF),forced vital capacity(FVC),peripheral blood eosinophils(EOS)and interleukin-17(IL-17)were compared between the two groups before and after treatment.[Results]After treatment,the traditional Chinese medicine(TCM)scores such as asthma,cough,expectoration,chest tightness,dyspnea and wheezing sound of lungs in the two groups were significantly improved,and the improvement in the observation group was superior to that in the control group.The difference was statistically significant(P<0.05).FEV1,PEF and FVC in the two groups were significantly improved after treatment,and the improvement in the observation group was superior to that in the control group.The difference was statistically significant(P<0.05);after treatment,the levels of EOS and IL-17 decreased in both groups,and the improvement in the observation group was superior to that in the control group,the difference was statistically significant(P<0.05).[Conclusions]The clinical effects of Acupoint Application of Jiezi Fangxiao Ointment combined with Pingchuan Prescription in the treatment of infantile asthma were obvious,which can significantly improve the TCM symptoms scores,pulmonary function and immune function of patients.

A REPORT ON THE TREATMENT OF STEROID RESISTANT ASTHMA WITH ACUPUNCTURE-MOXIBUSTION AND ACUPOINT INJECTION

In the present paper, the authors report their results of treatment of 5 cases of steriod resistant asthma with acupoint-injection, body acupuncture, moxibustion and cupping. Bilateral Zusanli (ST 36) are used for acupoint injection of mixture solution of placenta tissue injectio (2 mL), Danggui (Radix Angelicae Sinesis) injectio (2 mL) and Huangqi (Radix Astragli seu Hedysari) injectio (4 mL), with 4 mL for each acupoint. Acupoints used for puncturing are bilateral Yuji (LU 10), Dingchuan (EX-B 1), Feishu (BL 13), Fengmen (BL 12), Gaohuangshu (BL 43), Taiyuan (LU 9), Fenglong (ST 40), Shenshu (BL 23), Zusanli (ST 36), etc.. Dazhui (GV 14), Tanzhong (CV 17), and Zhongwan (CV 12) are moxibustioned after insertion of acupuncture needles and achieving needling sensations. Cupping is applied to bilateral Dingchuan (EX-B 1) after removing acupuncture needles with a little blood out. Following 3 days’ treatment, these 5 asthma patients who have no apparent response to conventional medication treatment are relieved strikingly in the attack frequency, till the 7th day of the treatment, they have no any asthmatic onset again. One month’s follow-up shows no recurrence. Results indicate that acupuncture with moxibustion and cupping is fairly effective in relieving steriod resistant asthma patients who have no effect in responding to glucocorticoid treatment.

THE FUNCTIONAL SIMILARITIES AND DIFFERENCES BETWEEN ACUPOINTS SHANZHONG AND QIHAI

Shangzhong (Ren 17) and Qihai (Ren6) are important acupoints located on theRen Channel (the Front Midline Channel),the former being on the chest and the latterbelow the umbilicus. Since both acupointsregulate the functional activities of qi (vitalenergy) and replenish primordial qi,Shanzhong (Ren 17) is known as the "

咳喘宁穴位敷贴、抗敏治咳方联合沙美特罗替卡松治疗咳嗽变异性哮喘临床研究

目的:观察咳喘宁穴位敷贴、抗敏治咳方联合沙美特罗替卡松治疗风寒型咳嗽变异性哮喘(CVA)的临床疗效。方法:将100例风寒型CVA患者按随机数字表法分为对照组和联合组各50例。对照组剔除4例,联合组剔除3例,最终纳入对照组46例、联合组47例。对照组给予沙美特罗替卡松粉吸入剂治疗8周,联合组给予沙美特罗替卡松粉吸入剂联合咳喘宁穴位敷贴治疗4周后,改予抗敏治咳方口服治疗4周。2组均随访12周。比较2组咳嗽症状积分,第1秒用力呼气容积(FEV_(1))、呼气峰值流速(PEF)、呼出气一氧化氮(FeNO)、嗜酸性粒细胞(EOS)、免疫球蛋白E (IgE)、白细胞介素-4 (IL-4)、肿瘤坏死因子-α (TNF-α)水平,以及复发率。评估治疗安全性。结果:治疗后,2组咳嗽症状积分均较治疗前降低(P<0.05);组间咳嗽症状积分比较,差异无统计学意义(P>0.05)。治疗后,2组FEV_(1)、PEF水平均较治疗前升高(P<0.05),FeNO水平均较治疗前降低(P<0.05);组间FEV_(1)、PEF水平比较,差异均无统计学意义(P>0.05);联合组FeNO水平低于对照组(P<0.05)。治疗后,2组血清EOS、IgE、IL-4、TNF-α水平均较治疗前降低(P<0.05),联合组上述4项炎症指标水平均低于对照组(P<0.05)。随访12周,联合组复发率8.51%,低于对照组23.91%(P<0.05)。治疗期间,对照组有5例、联合组有1例患者出现不良反应。结论:咳喘宁穴位敷贴、抗敏治咳方联合沙美特罗替卡松治疗风寒型CVA,可改善咳嗽症状,提升肺功能,减轻炎症状态,降低复发率。

健脾益气平喘方联合冬病夏治之穴位敷贴对缓解期支气管哮喘(肺气虚证)患者的作用效果

目的 探究健脾益气平喘方联合冬病夏治之穴位敷贴对缓解期支气管哮喘(肺气虚证)患者的作用效果。方法 以随机数字表法将安阳市中医院在2019年3月至2020年3月收治的110例缓解期支气管哮喘(肺气虚证)患者分为两组,接受冬病夏治之穴位敷贴治疗为对照组,接受健脾益气平喘方联合冬病夏治之穴位敷贴治疗为观察组,每组55例,3 a为1个治疗周期。比较两组临床疗效,治疗前后中医证候评分、血液流变学水平、免疫功能[γ干扰素(IFN-γ)、免疫球蛋白(IgE)、白细胞介素(IL)-4]以及抗氧化[超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)]水平。结果 观察组总有效率高于对照组(P<0.05);与治疗前比,治疗后两组中医证候评分均下降,观察组比对照组低(P<0.05);与治疗前比,治疗后两组高切、中切、低切全血黏度均下降,观察组比对照组低(P<0.05);与治疗前比,治疗后两组IL-4、IgE、MDA水平均下降,观察组比对照组低;IFN-γ、SOD、GSH-Px水平上升,观察组比对照组高(P<0.05)。结论 健脾益气平喘方联合冬病夏治之穴位敷贴治疗用于缓解期支气管哮喘(肺气虚证)患者,能够改善患者生活质量,缓解临床症状,改善机体微循环,调节免疫功能,提高机体抗氧化能力。

穴位贴敷配合督脉铺灸治疗肺肾气虚型哮病临床研究

目的:观察穴位贴敷配合督脉铺灸治疗肺肾气虚型哮病的临床疗效。方法:将90例患者随机分为治疗组、对照组和西药组,每组30例,治疗组采用穴位贴敷配合督脉铺灸治疗,对照组采用穴位贴敷治疗,西药组采用沙美特罗替卡松气雾剂治疗,于每年三伏期间治疗,连续治疗3年。治疗后观察3组临床疗效、肺功能、症状积分、哮喘控制测试评分及不良反应。结果:治疗结束后,治疗组、对照组与西药组在临床疗效、肺功能指标、症状评分、哮喘控制测试评分等方面比较,均有明显差异(P<0.05),且治疗组与对照组比较,也有明显差异(P<0.05)。3组不良反应比较,差异无统计学意义(P>0.05)。结论:穴位贴敷配合督脉铺灸治疗肺肾气虚型哮病可有效改善患者肺功能,提高临床疗效。

穴位贴敷联合中药治疗支气管哮喘急性发作期的疗效观察

目的观察白芥子散穴位贴敷联合麻龙定喘汤治疗支气管哮喘急性发作期的临床疗效。方法选取支气管哮喘急性发作期患者80例,随机分为研究组和对照组,每组40例。两组均予常规治疗,对照组采用口服麻龙定喘汤治疗,研究组在对照组基础上联合白芥子散穴位贴敷治疗。比较两组临床疗效和不良反应发生情况,观察两组治疗前后中医证候积分、肺功能指标、血清转化生长因子β1(transforming growth factor-β1,TGF-β1)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)水平的变化。结果研究组总有效率和显效率均高于对照组(P<0.05)。治疗后,研究组中医证候积分低于对照组(P<0.05),研究组用力肺活量(forced vital capacity,FVC)、呼气峰值流速(peak expiratory flow,PEF)及第一秒最大呼气容积(forced expiratory volume in 1 second,FEV1)均高于对照组(P<0.05),研究组血清TGF-β1和MMP-9水平均低于对照组(P<0.05)。两组不良反应发生率比较,差异无统计学意义(P>0.05)。结论在常规治疗基础上,白芥子散穴位贴敷联合麻龙定喘汤治疗能减轻支气管哮喘发作期患者咳嗽、喘息等临床症状,改善肺功能,减轻炎症反应,疗效优于单一中药治疗。

Influence of Acupoint Application Therapy on Biochemical Indexes of Asthma Patients in Remission Period

Objective： To observe the influence of acupoint application therapy on serum eosinophilic granulocyte cationic protein （ECP）, 6 keto prostaglandin F 1 （6-keto-PGF 1） and thromboxane B2 （TXB2）. Methods： 300 cases of asthma out-patients in a remission period were divided into a treatment group （150 cases） and a control group （150 cases）. The control group was given the basic treatment, and the treatment group was given external application on the acupoints in July and December every year, as well as the basic treatment of the control group, with continuous treatment for 2 years as one course. ECP, 6-keto-PGF 1, and TXB2 were checked every year in the patients of the two groups, and an Asthma Control Test Table was filled in for comparative analysis. Results： After the external application on the acupoints, the frequency of seizures, dyspnea and frequency of medication administration of the patients had been more reduced than those in the control group, indicating that the levels of ECP in inflammation of air passage and of 6-eto-GF 1 and TXB2 were obviously improved （P〈0.05）. Conclusion： The herbal application on the acupoints is effective and positively influential to the improvement of the symptoms in the asthma patients and to ECP, 6-keto-PGF 1, and TXB2 remarkably.

中医护理技术配合穴位敷贴对支气管哮喘缓解肺功能及不良反应的影响

目的研究中医护理技术配合穴位敷贴对支气管哮喘对缓解患者肺功能水平,降低不良反应的影响。方法我院2020年5月至2023年7月收治支气管哮喘患者70例,通过双盲法分成两组,各35例。对照组实施常规护理,观察组实施中医护理技术+穴位敷贴,比较两组干预1个月后,干预效果、肺功能的改善情况及发生的不良反应情况。结果观察组相较于对照组,总有效率更高,不良情绪改善更显著,肺部症状消失时间更短,肺功能各指标改善更显著,生活质量提升更显著,不良反应率更低,组间比较有统计学意义(P<0.05)。结论支气管哮喘患者临床治疗过程中,通过中医护理配合穴位敷贴的技术支持,可以缓解患者的肺功能水平,提升患者生活质量同时,也能有效降低不良反应率发生,确保患者临床治疗的安全性,适于临床推广。

穴位推拿联合布地奈德+特布他林雾化吸入治疗支气管哮喘患儿的效果及对炎症因子水平的影响

目的探讨穴位推拿联合布地奈德+特布他林雾化吸入治疗支气管哮喘患儿的效果及对炎症因子水平的影响。方法选取2021年1月至2022年1月收治的64例支气管哮喘患儿,采用随机数字表法将其分为A组(32例,布地奈德+特布他林雾化吸入治疗)和B组(32例,在A组基础上实施穴位推拿)。比较两组的治疗效果。结果治疗后,两组的喘息、呼吸困难、咳嗽及肺部湿啰音评分降低,且B组低于A组(P<0.05)。治疗后,两组的儿童哮喘控制测试问卷(C-ACT)评分、第1秒用力呼气容积占预计值的百分比(FEV1%)、最大呼气峰流速度占预计值的百分比(PEF%)高于治疗前(P<0.05);治疗后,B组的C-ACT评分、FEV1%和PEF%高于A组(P<0.05)。治疗后,两组的嗜酸性粒细胞(EOS)计数及免疫球蛋白E(IgE)、神经营养因子-3(NT-3)水平低于治疗前(P<0.05);治疗后,B组的EOS计数及IgE、NT-3水平低于A组(P<0.05)。随访6个月,两组的哮喘复发率无显著差异(P>0.05)。结论穴位推拿联合布地奈德+特布他林雾化吸入治疗支气管哮喘患儿的效果显著,可改善临床症状和肺功能,降低炎症因子水平,值得临床推广应用。

宣肺平喘汤加减结合穴位贴敷治疗咳嗽变异性哮喘风寒袭肺证的效果及对气道重塑的影响

目的探讨宣肺平喘汤加减结合穴位贴敷治疗咳嗽变异性哮喘风寒袭肺证的效果及对气道重塑的影响。方法选取2020年4月至2022年8月治疗的100例咳嗽变异性哮喘风寒袭肺证患者为研究对象,按照随机数字表法将其分为对照组与观察组,各50例。对照组接受常规西药治疗,观察组在对照组基础上加宣肺平喘汤加减结合穴位贴敷治疗。比较两组的治疗效果。结果观察组的治疗总有效率高于对照组(P<0.05)。治疗后,观察组的嗜酸性粒细胞计数(EOS)、白细胞介素-4(IL-4)水平低于对照组,干扰素-γ(IFN-γ)水平高于对照组(P<0.05)。治疗后,观察组的呼出气一氧化氮(FeNO)、肺泡气一氧化氮(CaNO)低于对照组(P<0.05)。治疗后,观察组的结缔组织生长因子(CTGF)、人软骨糖蛋白-39(HC-gp39)、基质金属蛋白酶抑制因子-1(TIMP-1)及基质金属蛋白酶-9(MMP-9)水平低于对照组(P<0.05)。结论宣肺平喘汤加减结合穴位贴敷治疗咳嗽变异性哮喘风寒袭肺证的效果满意,可调节炎性因子指标,降低FeNO、CaNO,抑制气道重塑。

祛风宣肺健脾方联合穴位贴敷在风邪犯肺型咳嗽变异性哮喘患儿治疗中的效果观察

目的探究祛风宣肺健脾方联合穴位贴敷在风邪犯肺型咳嗽变异性哮喘(CVA)中的临床疗效.方法选取2021年1月—2023年1月寿光市人民医院东院区收治的82例风邪犯肺型CVA患儿为研究对象,按随机数字表法将其分为对照组与研究组,各41例.对照组采用穴位贴敷治疗,观察组在对照组基础上采用祛风宣肺健脾方治疗,连续治疗1个月.对比两组临床疗效、临床相关指标、炎性因子及免疫功能指标.结果治疗后,相较于对照组,观察组治疗总有效率较高,咳嗽缓解时间(3.08±0.74)d、咳嗽消失时间(5.48±1.22)d及肺啰音消失时间(5.82±1.23)d均较短,白细胞介素-5水平(24.08±4.66)ng/mL、肿瘤坏死因子-α水平(3.03±0.63)ng/mL均较低,转化生长因子-β1水平(72.15±8.97)ng/L较高,免疫球蛋白E水平(229.22±35.61)IU/mL及嗜酸性粒细胞水平(0.36±0.04)×10^(9)L均较低,组间差异有统计学意义(P<0.05).结论祛风宣肺健脾方与穴位贴敷临床疗效显著,可有效缓解风邪犯肺型CVA患儿临床症状,且有助于提高患儿机体免疫功能,缓解机体炎症反应.

发酵白芥子穴位敷贴对支气管哮喘大鼠气道炎症和Th17/Treg免疫失衡的影响

目的 探究发酵白芥子穴位敷贴对支气管哮喘大鼠气道炎症和辅助性T细胞17(Th17)/调节性T细胞(Treg)免疫失衡的影响。方法 将48只SPF级SD大鼠随机分为空白组、模型组、地塞米松组、发酵组、非发酵组及假贴组,每组8只。空白组用生理盐水代替干预,其余组大鼠均用卵清蛋白和氢氧化铝混合液致敏建立支气管哮喘模型。造模成功后,每天雾化激发1 h前,地塞米松组给予腹腔注射地塞米松1 mg/kg,1次/d,共10次;发酵组、非发酵组将药物贴敷于大鼠大椎、膻中、肺俞(双侧)、脾俞(双侧)、肾俞(双侧)穴,每次5 h,1次/d,共10次;假贴组用同样方法贴敷凡士林;空白组和模型组不做任何处理。于末次雾化激发时观察各组大鼠哮喘行为并计分;干预结束后,取肺组织行HE染色观察其病理形态,取大鼠血清和肺泡灌洗液,ELISA法检测Th17、Treg相关细胞因子白细胞介素-17(IL-17)、白细胞介素-22(IL-22)、白细胞介素-10(IL-10)和白细胞介素-35(IL-35)水平,Western blot法检测肺组织中IL-17、IL-22、IL-10、IL-35蛋白表达情况。结果 与空白组比较,模型组、假贴组大鼠支气管黏膜上皮细胞受损脱落,管腔明显狭窄,气道壁不完整,肺泡内炎症细胞浸润明显,肺泡间隔明显增厚;哮喘行为学评分、血清及肺泡灌洗液中IL-17和IL-22水平、肺组织中IL-17和IL-22蛋白相对表达量均明显升高(P均<0.05),血清及肺泡灌洗液中IL-10和IL-35水平、肺组织中IL-10和IL-35蛋白相对表达量均明显降低(P均<0.05)。与模型组和假贴组比较,地塞米松组、发酵组、非发酵组大鼠支气管上皮较完整,偶见上皮细胞脱落,支气管黏膜偶见炎性细胞浸润,肺间质轻微增生;哮喘行为学评分、血清及肺泡灌洗液中IL-17和IL-22水平、肺组织中IL-17和IL-22蛋白相对表达量均明显降低(P均<0.05),血清及肺泡灌洗液中IL-10和IL-35水平、肺组织中IL-10和IL-35蛋白相对表达量均明显升高(P均<0.05);地塞米松组、发酵组、非发酵组间肺组织病理形态、血清及肺泡灌洗液中各炎症指标水平和肺组织中蛋白表达量比较差异均无统计学意义(P均>0.05)。结论 发酵白芥子穴位敷贴能有效减轻支气管哮喘大鼠哮喘发作症状和气道炎症,降低体内炎性细胞因子水平,调节Th17/Treg免疫失衡状态。

降气平喘固本汤联合穴位推拿治疗儿童支气管哮喘虚哮证的临床效果及对其免疫功能的影响

目的探讨降气平喘固本汤联合穴位推拿治疗儿童支气管哮喘虚哮证的效果。方法选取2018年11月—2023年6月期间北京中医医院怀柔医院收治的支气管哮喘虚哮证患儿102例作为研究对象,依据简单随机数字表法分为对照组和研究组,每组各51例。对照组采取常规西医治疗,研究组在对照组基础上采取降气平喘固本汤联合穴位推拿,共治疗4周。观察比较两组患儿临床疗效、不良反应发生率,治疗前后中医证候积分、肺功能[最大呼气峰流速(Peak expiratory flow,PEF)、第1秒用力呼气量(Forced expiratory volume in one second,FEV1)、用力肺活量(Forced vital capacity,FVC)]、免疫功能[白细胞介素-13(Interleukin-13,IL-13)、白细胞介素-12(Interleukin-12,IL-12)、白细胞介素-4(Interleukin-4,IL-4)、干扰素-γ(Interferon-gamma,IFN-γ)]水平。结果治疗后研究组临床总有效率92.16%(47/51)明显高于对照组78.43%(40/51),差异有统计学意义(P<0.05)。治疗后两组患儿喉中哮鸣、形寒肢冷、咳痰无力、腰膝酸软、神疲乏力、声低气短分值均较治疗前降低,差异有统计学意义(P<0.05);且研究组中医证候积分明显低于对照组,差异有统计学意义(P<0.05)。治疗后两组患儿肺功能PEF、FEV1、FVC水平均较治疗前升高,差异有统计学意义(P<0.05);且研究组肺功能PEF、FEV1、FVC水平均明显高于对照组,差异有统计学意义(P<0.05)。治疗后两组患儿免疫功能IL-13、IL-4水平均较治疗前降低,IL-12、IFN-γ水平均较治疗前升高,差异有统计学意义(P<0.05);且研究组免疫功能IL-13、IL-4水平均明显低于对照组,IL-12、IFN-γ水平均明显高于对照组,差异有统计学意义(P<0.05)。治疗期间,研究组不良反应发生率5.88%(3/51)与对照组3.92%(2/51)比较,差异无统计学意义(P>0.05)。结论采取降气平喘固本汤联合穴位推拿治疗支气管哮喘虚哮证,可有效缓解患儿临床症状,提升治疗效果,且具有安全性。