A Guide to Cheek Augmentation: Single-Point Deep Injection of Hyaluronic Acid Filler at Midface in Close Proximity to Medial Suborbicularis Oculi Fat (SOOF) Area

Loss of volume in midface can result in an aged, wasted appearance. Osseous and fat atrophy with aging may further contribute to the loss of soft tissue support and midface ptosis. In the aging of periorbital area and midface, fat atrophy occurs mostly in the suborbicularis oculi fat (SOOF) area. The authors proposed that injection of hyaluronic acid (HA) filler to support the SOOF area could counteract the aging sign due to fat atrophy, restore volume loss and achieve a more youthful appearance. The authors described the treatment of 10 female patients who received CHAP®-particle hyaluronic acid (CHAP®-HA) injections for cheek augmentation, using single-point deep injection technique at midface in close proximity to SOOF area. Such approach provides satisfactory cheek augmentation results without significant complications. The authors discussed a rationale for their choice of dermal filler and provided an injection technique for restoring volume in the midface region with CHAP®-HA. Such technique is relatively quick to perform, have little down time, and result in a high rate of patient satisfaction.

Majocchi’s granuloma caused by Trichophyton rubrum after facial injection with hyaluronic acid:A case report

BACKGROUND Facial cosmetic procedures become popular for people with a desire to have a younger appearance,and cosmetic technology has developed rapidly over the past several decades.However,increasing complications related to cosmetic injections have been reported,and infection is one of the most serious problems and can cause anxiety and facial injury.We here report a case of Majocchi’s granuloma(MG)caused by Trichophyton rubrum after facial injection of hyaluronic acid.CASE SUMMARY A 37-year-old woman presented to our hospital with a history of red papules,nodules,and abscesses on her left zygomatic arch for 2 mo.She had received a cosmetic injection of hyaluronic acid on the left side of her face prior to the appearance of the lesions.MG caused by Trichophyton rubrum after facial injection of hyaluronic acid was diagnosed based on morphology and molecular biological identification.In vitro antifungal susceptibility testing was conducted according to the Clinical and Laboratory Standards Institute M38-A2 method.Minimal inhibitory concentrations were used to evaluate the antifungal susceptibility.The antifungal agents and their minimal inhibitory concentrations for the strain were terbinafine(<0.5μg/mL),itraconazole(0.06μg/mL),amphotericin B(0.25μg/mL),fluconazole(32μg/mL),voriconazole(0.125μg/mL),posaconazole(0.125μg/mL),and isavuconazole(0.06μg/mL).We initially administered 250 mg/d oral terbinafine for 2 mo,but the patient still had painful papules,nodules and abscesses on her face.Then,we adjusted the treatment to itraconazole 400 mg/d for 8 wk based on the in vitro antifungal susceptibility testing results.The skin lesions improved significantly,and there was no recurrence during follow-up.CONCLUSION This case revealed that facial injection of hyaluronic acid may cause serious MG.Antifungal susceptibility testing should be considered in the treatment of MG caused by Trichophyton rubrum.

Use of hyaluronic acid for sperm immobilisation and selection before intracytoplasmic sperm injection:A systematic review and meta-analysis

AIM:To appraise critically the published randomised controlled trials(RCTs)reporting on the effectiveness of using hyaluronic acid(HA)for sperm immobilisation and selection before intracytoplasmic sperm injection(ICSI).METHODS:Two authors used the PICO Method in order to perform a comprehensive literature search of the standard medical databases in June 2015.Data from the included studies was extracted independently by two authors using a predefined pro-forma.Review Manager(RevM an)was used to calculate the combined outcomes where multiple studies contributed with their results.Risk ratio(RR)with a 95%CI using the Mantel-Haenszel method was calculated for binary data variables.Heterogeneity was measured using the χ2 test and quantified using I2.In case of substantial heterogeneity(P < 0.10 for χ2 test or I2 > 50%)the combined outcome was calculated using the random effects model.The results from the meta-analysis were displayed as forest plots.The guideline of the Cochrane Collaboration was used to assess the risk of bias and it was illustrated as a risk of bias graph.RESULTS:The systematic literature search identified 166 different studies related to sperm immobilisation and selection for ICSI.Eleven RCTs involving 13719 oocyte intracytoplasmatic injections with sperm immobilised and selected using HA or polyvinylpyrrolidone(PVP)were included in this systematic review and metaanalysis.There was low heterogeneity among the included trials(χ2 = 16.86,df = 11,P = 0.11; I2 = 35%).There was no statistical difference between HA and PVP groups in terms of fertilisation rate(RR = 1.01; 95%CI:0.99-1.03; z = 0.75; P = 0.45),good embryos rate(RR = 1.01; 95%CI:0.96-1.06; z = 0.30; P = 0.76),live birth rate(RR = 1.15; 95%CI:0.86-1.54; z = 0.92; P = 0.36),clinical pregnancy rate(RR = 1.04; 95%CI:0.92-1.17; z = 0.62; P = 0.53)and implantation rate(RR = 1.17; 95%CI:0.94-1.46; z = 0.40; P = 0.16).The quality of most of the included studies was moderate to poor because of unclear randomisation technique,inadequate allocation concealment and blinding.CONCLUSION:This systematic review and metaanalysis provides evidence of similar efficiency between using HA or PVP for sperm immobilisation and selection before ICSI.

Intraarticular injections(corticosteroid, hyaluronic acid, platelet rich plasma) for the knee osteoarthritis

Osteoarthritis(OA)is a complex"whole joint"disease pursued by inflammatory mediators,rather than purely a process of"wear and tear".Besides cartilage degradation,synovitis,subchondral bone remodeling,degeneration of ligaments and menisci,and hypertrophy of the joint capsule take parts in the pathogenesis.Pain is the hallmark symptom of OA,but the extent to which structural pathology in OA contributes to the pain experience is still not well known.For the knee OA,intraarticular(IA)injection(corticosteroids,viscosupplements,blood-derived products)is preferred as the last nonoperative modality,if the other conservative treatment modalities are ineffective.IA corticosteroid injections provide short term reduction in OA pain and can be considered as an adjunct to core treatment for the relief of moderate to severe pain in people with OA.IA hyaluronic acid(HA)injections might have efficacy and might provide pain reduction in mild OA of knee up to 24 wk.But for HA injections,the costeffectiveness is an important concern that patients must be informed about the efficacy of these preparations.Although more high-quality evidence is needed,recent studies indicate that IA platelet rich plasma injections are promising for relieving pain,improving knee function and quality of life,especially in younger patients,and in mild OA cases.The current literature and our experience indicate that IA injections are safe and have positive effects for patient satisfaction.But,there is no data that any of the IA injections will cause osteophytes to regress or cartilage and meniscus to regenerate in patients with substantial and irreversible bone and cartilage damage.

Use of High-Resolution Ultrasound (HRU) in the Assessment of Deep Injections of CHAP-Hyaluronic Acid (CHAP-HA) Fillers for Midface Lift

High-resolution ultrasound (HRU) imaging is a useful tool to study hyaluronic acid (HA) filler injection in the face. It is noninvasive, quick, well-tolerated, and can provide in vivo and dynamic information. The formations of pools or pearls in HA fillers could be observed real time during injection. The plane of injection could be determined accurately, and there were no specimen manipulation artifacts. It was observed that HA gel fillers with differing production technologies showed distinct spread and distribution patterns in the periocular tissues on HRU examination. The authors used HRU to assess deep injections of CHAP-Hyaluronic Acid (CHAP-HA) fillers for midface lift. 10 patients who underwent bilateral midface deep injections using CHAP-HA filler were examined with HRU before and immediately after treatment, and in 2 weeks and one month later. The CHAP-HA appeared as hypoechoic densities within the preperiosteal plane in HRU. CHAP-HA adopted variable morphology within the tissue depending on individual tissue densities and the compliance of the tissues in the plane of injection. CHAP-HA was unidentifiable with surrounding tissue after one month in 13 of the 20 injection sites. HRU allows in vivo study of CHAP-HA injection behavior and could be a tool for further studies of HA-tissue reactions.

No difference in high-magnification morphology and hyaluronic acid binding in the selection of euploid spermatozoa with intact DNA

In this study, we compared conventional sperm selection with high-magnification morphology based on the motile sperm organellar morphology examination （MSOME） criteria, and hyaluronic acid （HA） binding for sperm chromosome aneuploidy and DNA fragmentation rates. Semen from 50 severe male factor cases was processed through density gradient centrifugation, and subjected to sperm selection by using the conventional method （control）, high magnification at x6650 or HA binding. Aneuploidy was detected by fluorescence in situ hybridization with probes for chromosomes 13, 18, 21, X and Y, and DNA fragmentation by the terminal deoxynucleotidyl transferase dUTP nick end labelling （TUNEL） method. Spermatozoa selected under high-magnification had a lower DNA fragmentation rate （2.6% vs. 1.7%; P=0.032）, with no significant difference in aneuploidy rate （0.8% vs0.7%; P=0.583）, than those selected by the HA binding method. Spermatozoa selected by both methods had much lower aneuploidy and DNA fragmentation rate than the controls （7% aneuploidy and 26.8% DNA fragmentation rates, respectively）. In the high-magnification group, the aneuploidy rate was lower when the best spermatozoa were selected than when only the second-best spermatozoa were available for selection, but the DNA fragmentation rate was not different. In conclusion, sperm selection under high magnification was more effective than under HA binding in selecting spermatozoa with low DNA fragmentation rate, but the small difference （0.9%） might not be clinically meaningful. Both methods were better than the conventional method of sperm selection.

Preparation and Evaluation of an Injectable Chitosan-Hyaluronic Acid hydrogel for Peripheral Nerve Regeneration

The aim of this study was to obtain the fillers in the lumen of hollow nerve conduits（NCs） to improve the microenvironment of nerve regeneration. A p H-induced injectable chitosan（CS）-hyaluronic acid（HA） hydrogel for nerve growth factor（NGF） sustained release was developed. Its properties were characterized by gelation time, FT-IR, SEM, in vitro swelling and degradation. Furthermore, the in vitro NGF release profiles and cell biocompatibility were also investigated. The experimental results show that the CS-HA aqueous solution can undergo a rapid gelation 3 minutes after its environmental p H is changed to 7.4. The CSHA hydrogel has interconnected channels with a controllable pore diameter and with a porosity of about 80%. It has a favorable swelling behavior and can be degraded by about 70% within 8 weeks in vitro and is suitable for NGF release. The CS-HA/NGF hydrogel exhibits a lower cytotoxicity and is in favor of the adhesion and proliferation of the BMMSCs cells. It is indicated that the CS-HA/NGF will be a promising candidate for neural tissue engineering.

Pyrogenicity of hyaluronic acid hydrogel crosslinked by divinyl sulfone for soft tissue augmentation

Hyaluronic acid hydrogels (HAHs) were synthesized by immersing the microbeads in phosphate buffered saline solution to assess short term biocompatibility of the gels by means of the rabbit pyrogen test and the bacterial endotoxin test. The rise in body temperature of 3 male New Zealand white rabbits weighing about 2~3 kg (12~16 weeks old) following intravenous injection of the test article (10 mL/kg) was mo nitored at 30 min intervals in 3 h to examine the pyrogenicity. No rabbits showed an individual rise in temperature of 0.5oC or more above its respective control temperature. The temperature rises of the rabbits after injection were 0.12oC, 0.13oC, and 0.18oC, respectively, sugge sting that HAH meets the requirements for the absence of pyrogens. The bacterial endotoxin test revealed that the concentration of endoto xins required to cause the lysate to clot under standard conditions was < 0.125 EU/mL. Comparing the HAHs that was synthesized in this experiment to the ones approved by FDA, the amount of < 0.125 EU/mL endotoxins is relatively safe and effective. The test solution did not contain any interfering factors under the ex perimental conditions used. It is conceivable that the HAHs are likely to be suitable injectable dermal filler for facial soft tissue augmentation due to the absence of

Viscoelastic Evaluation of Different Hyaluronic Acid Based Fillers Using Vibrational Optical Coherence Tomography

Hylauronic acid (HA) is used as a viscoelastic in Ophthalmology during cataract surgery based on its high viscosity at rest, its ability to shear thin and dissipate energy during phacoemulsification. However, these properties of HA solutions would make them susceptible to migration when used as dermal filler materials. In this study, we apply a new technique termed vibrational optical coherence tomography (VOCT) to compare the physical properties of different HA solutions and fillers used in facial aesthetics. Results presented in this study suggest that HA solutions and HA dermal fillers have markedly different physical properties. HA solutions are highly viscoelastic with high % viscous losses while fillers tend to have lower viscous energy dissipation properties. Clinical observations suggest that the high loss fillers are injected more superficially in the face where tension and internal and external forces are more likely minimized giving tissue of the hands and lips more volume and allowing more natural movement. In contrast, the lower loss gels that are used to lift tissue, generally have a higher G’, and are injected deeper into the face where injection and internal forces are likely to be higher. It is concluded that HA filler gel design can be optimized by use of VOCT to evaluate the % viscous energy loss both in vitro and in vivo.

Intraplaque injections of hyaluronic acid for the treatment of stable-phase Peyronie’s disease:a retrospective single-center experience

Peyronie’s disease(PD)is a condition of penile connective tissue affecting up to 10%of men worldwide.In the complexity ofits management,nonsurgical treatments,such as intraplaque injections,are gaining attention.The current literature shows dataon the efficacy of intraplaque injections of hyaluronic acid(HA)mainly in acute-phase PD.However,data on injections of HA instable-phase PD are lacking.Data for this retrospective study were derived from a prospectively maintained database of privatepatients presenting at a private medical practice affiliated to the University of Naples“Federico II”(Naples,Italy)with stable-phasePD between January 2020 and March 2023.Patients underwent a standard protocol of three injections,each administered at atwo-week interval.During the intervals,patients performed vacuum device therapy,penile stretching,and modeling exercises.Allpatients compiled the Peyronie’s Disease Questionnaire(PDQ)and Global Assessment of Peyronie’s Disease(GAPD)at baselineand 2 weeks after the third injection.A penile Doppler ultrasound was performed 2 weeks after the last injection to record thefinal curvature.Overall,we recruited 62 patients with stable-phase PD and a mean(±standard deviation[s.d.])curvature of52.7°(±9.7°).After 6 weeks,eight(12.9%)patients did not experience any curvature improvement.The remaining 54 patientshad a final mean(±s.d.)curvature of 40.3°(±9.1°)with P<0.001,compared to that before treatment.We found improvement inall PDQ domains(all P≤0.01),and 50(80.6%)patients reported subjective improvement of the penile curvature according to theGAPD.In conclusion,we demonstrated that after three injections of HA administered according to the adopted protocol,patientswith stable-phase PD could experience significant improvements in penile curvature,and physical and psychological consequencesof the disease without significantly relevant side effects.

Complications and management of penile augmentation with hyaluronic acid injection

Hyaluronic acid injection is becoming a popular way for penile augmentation.However,only few studies and follow-ups have investigated the various complications of hyaluronic acid injection and their corresponding management.In this study,a total of 230 patients who had penile augmentation with hyaluronic acid injection from January 2018 to December 2019 were examined on follow-up for penile girth,complications,and their corresponding management.At 1-month,3-month,and 6-month postoperative follow-ups,the penile circumference had increased by 2.66±1.24 cm,2.28±1.02 cm,and 1.80±0.83 cm,respectively.During the entire 6-month follow-up,4.3%had complications such as subcutaneous bleeding,subcutaneous nodules,and infection.There were no systemic or local allergic reactions among all the patients.All complications were treated accordingly,and no further deterioration or severe sequelae were observed.Although complications of hyaluronic acid injections are mild and rare,these may affect the patient's satisfaction postoperatively.Preoperative redundant prepuce may increase the incidence of penile edema or postoperative gel migration.Standardization of the surgery protocol and elucidation of the effects of other injection parameters are still lacking.Nevertheless,it still highlights the importance of preoperative preparation and surgical technique.

Injectable Hyaluronic Acid/Poly(y-glutamic acid)Hydrogel with Step-by-step Tunable Properties for Soft Tissue Engineering

Injectable hydrogels as an important class of biomaterials have gained much attention in tissue engineering.However,their crosslinking degree is difficult to be controlled after being injected into body.As we all know,the crosslinking degree strongly influences the physicochemical properties of hydrogels.Therefore,developing an injectable hydrogel with tunable crosslinking degree in vivo is important for tissue engineering.Herein,we present a dual crosslinking strategy to prepare injectable hydrogels with step-by-step tunable crosslinking degree using Schiff base reaction and photopolymerization.The developed hyaluronic acid/poly(y-glutamic acid)(HA/y-PGA)hydrogels exhibit step-by-step tunable swelling behavior,enzymatic degradation behavior and mechanical properties.Mechanical performance tests show that the storage moduli of HA/y-PGA hydrogels are all less than 2000 Pa and the compressive moduli are in kilopascal,which have a good match with soft tissue.In addition,NIH 3T3 cells encapsulated in HA/y-PGA hydrogel exhibit a high cell viability,indicating a good cytocompatibility of HA/y-PGA hydrogel.Therefore,the developed HA/y-PGA hydrogel as an injectable biomaterial has a good potential in soft tissue engineering.

阴茎头局部注射透明质酸治疗早泄的方法详解和经验分享

早泄(PE)是男性常见的射精功能障碍,发病率较高,是导致患者及其伴侣性满意度低的常见原因。其病因复杂,受到心理、解剖、神经生物和行为医学等因素影响,难以彻底治愈。目前针对PE的治疗,除了心理干预、局部麻醉剂、达泊西汀等药物和手术治疗外,透明质酸(HA)凝胶注射也是一种可替代的治疗方法。该方法通过阴茎头局部皮下注射HA凝胶来阻断神经末梢的感觉传入,降低阴茎头的敏感性,延长患者阴道内射精潜伏时间,提高患者及其伴侣性满意度。HA凝胶具有安全性及耐受性高等优点,是一种有前景的临床应用材料。本文在探讨PE的其他疗法的同时,详细介绍了HA凝胶注射治疗的注射技术、有效性和安全性。

关节腔冲洗联合液态浓缩生长因子注射治疗单侧颞下颌关节骨关节炎的初步研究

目的:观察关节腔冲洗联合液态浓缩生长因子(concentrated growth factor, CGF)注射治疗单侧颞下颌关节骨关节炎(temporomandibular joint osteoarthritis, TMJOA)的短期临床效果,以期为TMJOA患者提供新的治疗选择。方法:采用非随机对照研究,选择2021年6月至2023年1月于北京大学口腔医院颞下颌关节病及口颌面疼痛诊治中心就诊的单侧TMJOA患者作为研究对象,分为试验组和对照组,由患者自行选择入组。试验组行关节腔冲洗联合液态CGF注射治疗,对照组行关节腔冲洗联合透明质酸(hyaluronic acid, HA)注射治疗,分别治疗3次,每两周1次。治疗完成后6个月通过最大自主张口度、疼痛值和下颌功能受限程度等指标评价临床治疗效果,通过治疗前后的锥形束CT(cone beam CT,CBCT)图像融合技术评价髁突骨质的变化。结果:试验组纳入20例患者,其中男性3例,女性17例,平均年龄(34.40±8.41)岁;对照组纳入15例患者,其中男性1例,女性14例,平均年龄(32.20±12.00)岁,两组患者一般资料差异无统计学意义(P>0.05)。两组患者治疗前的张口度、疼痛值及下颌功能受限程度差异均无统计学意义(P>0.05),治疗后上述指标较治疗前均有所改善(P<0.05),但试验组患者治疗后的张口度显著大于对照组(P<0.05),下颌功能受限程度显著低于对照组(P<0.05)。CBCT二维图像显示两组患者治疗后的髁突骨质均较治疗前光滑,图像融合分析显示试验组10例(50.0%)患者和对照组5例(33.3%)患者的髁突出现修复改建区域,两者之间差异无统计学意义(P>0.05)。除1例试验组患者外,两组其余患者的髁突均有部分区域吸收。结论:关节腔冲洗联合液态CGF注射在短期内可以改善单侧TMJOA患者的临床症状和体征,且在增加张口度和改善下颌功能方面优于HA对照组。两组患者的CBCT融合图像均显示部分病例有髁突骨质修复改建的表现,但其与治疗方案的相关性仍需要扩大样本量进一步研究。

自体脂肪移植联合水光注射微交联透明质酸在面部年轻化中的应用及对皮肤状态、面部轮廓的影响

目的分析自体脂肪移植联合水光注射微交联透明质酸在面部年轻化中的应用效果。方法按照随机数字表法将于西安医学院第二附属医院美容科要求面部年轻化的60例患者分为对照组和研究组,各30例。对照组接受水光注射微交联透明质酸治疗,研究组在对照组基础上接受自体脂肪移植治疗。比较两组的手术效果。结果研究组的面部轮廓治疗总有效率显著高于对照组(P<0.05)。术后,研究组的皮肤弹性、皮肤含水量、经水皮肤流失、皮脂和黑色素明显优于对照组(P<0.05)。术后,研究组的基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-12(MMP-12)及活性氧簇(ROS)水平低于对照组(P<0.05)。两组的不良反应总发生率比较,差异无统计学意义(P>0.05)。术后,研究组的生活质量综合评定问卷(GQOLI-74)评分高于对照组(P<0.05)。结论自体脂肪移植联合水光注射微交联透明质酸对于改善面部年轻化有较好效果,能够改善患者的面部轮廓、皮肤状态和血清指标,且治疗相对安全。

氨甲环酸联合非交联透明质酸皮内注射治疗黄褐斑的应用效果

目的观察氨甲环酸联合非交联透明质酸皮内注射治疗黄褐斑的应用效果。方法选取2022年1月至2023年12月于广东省妇幼保健院进行就诊的黄褐斑患者145例,按照治疗方法不同将其分为注射组(n=46),口服组(n=50),联合组(n=49),注射组采用非交联透明质酸进行治疗,口服组采用口服氨甲环酸进行治疗,联合组采用氨甲环酸联合非交联透明质酸皮内注射进行治疗,比较三组患者治疗疗效、脸部黄褐斑的严重程度、脸部色斑分布情况以及不良反应发生率。结果治疗后,联合组患者基本痊愈率高于注射组和口服组患者(P<0.05),联合组效果优于注射组和口服组(P<0.05),三组患者的色斑分布情况均较治疗前改善(P<0.05),且联合组效果优于注射组和口服组(P<0.05)。三组患者的总发生率差异无统计学意义(P>0.05)。结论氨甲环酸口服联合非交联透明质酸皮内注射治疗能更有效地改善患者面部的黄褐斑的严重程度及其分布情况,且其疗效显著,无额外的不良反应发生,可广泛使用。

解郁清痤汤联合透明质酸注射治疗肝郁血热型玫瑰痤疮的临床观察

目的:探讨解郁清痤汤联合透明质酸注射治疗肝郁血热型面部玫瑰痤疮的疗效。方法:选取2020年3月-2022年3月于笔者医院被诊断为肝郁血热型面部玫瑰痤疮的80例患者为研究对象,随机进行分组。对照组(n=40)给予透明质酸注射治疗,研究组(n=40)在对照组的基础上联合解郁清痤汤治疗,观察两组治疗效果。结果:研究组疗效总有效率高于对照组(P<0.05);治疗后,研究组患者红斑、毛细血管扩张、丘疹等中医证候积分低于对照组(P<0.05);痤疮特异性生活质量量表(Acne-QoL)各方面评分均高于对照组(P<0.05)。结论:解郁清痤汤联合透明质酸注射治疗肝郁血热型面部玫瑰痤疮患者疗效显著,能够明显改善患者红斑、毛细血管扩张、丘疹、瘙痒等症状,提高生活质量。

中下面部线雕联合局部注射填充在面部年轻化中的应用

目的:探究中下面部线雕联合局部注射填充在面部年轻化中的应用价值。方法:选择2019年-2021年12月笔者医院皮肤科收治的需年轻化治疗的112例女性就医者,按随机数字表法分为对照组(56例)和观察组(56例)。对照组采用透明质酸局部注射填充,观察组采用中下面部线雕联合透明质酸局部注射填充,比较两组临床疗效、皮肤状态、面部皱纹改善情况、生活质量及不良反应。结果:术后1周,观察组临床疗效优良率高于对照组(P<0.05);术后3个月,观察组水分、弹性评分高于对照组,油脂评分低于对照组(均P<0.05),改良Fitzpatrick皱纹量表、整体美学改善量表(Global aesthetic improvement scale,GAIS)评分均优于对照组(P<0.05),观察组心理功能、社会功能、物质生活状态评分均高于对照组(P<0.05);两组不良反应发生率比较差异无统计意义(P>0.05)。结论:中下面部线雕联合局部注射填充抗衰效果显著,可进一步提高临床疗效,改善皮肤质地、皱纹等,不良反应轻微,美学效果好。

注射用透明质酸钠复合溶液中氨基酸含量测定

目的 建立氨基酸分析仪测定注射用透明质酸钠复合溶液中脯氨酸、甘氨酸和丙氨酸含量的方法。方法供试品中的氨基酸经阳离子交换树脂柱分离,于135℃下与茚三酮进行柱后衍生化显色反应,脯氨酸的检测波长为440 nm,其他氨基酸的检测波长为570 nm,外标法计算各氨基酸含量。结果 脯氨酸、甘氨酸和丙氨酸线性范围分别为0.0148~0.074,0.0207~0.103,0.0247~0.1235 mg/ml(r^(2)=0.9998);平均回收率分别为98.2%,98.5%和98.3%,RSD分别为0.55%,0.22%和1.40%;定量限分别为13.28,0.828,0.988μg。结论 方法重现性好、灵敏度高、检测结果准确且操作简单,适用于注射用透明质酸钠复合溶液中氨基酸含量的测定。

透明质酸无针水光注射配合黄金微针射频治疗皮肤老化的效果观察

目的探析透明质酸无针水光注射配合黄金微针射频治疗皮肤老化的效果。方法回顾性分析2021年7月~2023年12月来桂林市人民医院皮肤科要求改善面部皮肤老化的就医者60例,按治疗方式的差异将其分为A组与B组,各30例。A组患者采用单独黄金微针射频治疗,B组患者采用黄金微针射频联合透明质酸无针水光注射治疗(于射频治疗后第1周、第2周、第3周、第4周给予透明质酸无针水光注射)。两组患者均间隔3个月进行一次黄金微针射频治疗,2次为1个疗程。比较两组临床疗效、面部年轻化程度[面部皱纹量表(FWS)、皱纹严重程度评价量表(WSRS)]、面部VISIA评分、不良反应。结果治疗24周结束时,B组有效率高于A组(P<0.05);治疗24周,两组FWS、WSRS评分均高于治疗前,且B组更高(P<0.05);两组治疗前VISIA评分比较,差异无统计学意义(P>0.05);治疗后4周、治疗后12周、治疗后24周两组VISIA评分高于治疗前,且B组治疗后4周、治疗后12周、治疗后24周VISIA评分均高于A组(P<0.05);两组患者不良反应比较,无明显差异(P>0.05)。结论透明质酸无针水光注射配合黄金微针射频治疗皮肤老化的疗效确切,可显著实现面部年轻化。