[Objective] The research aimed to study the tissue culture technology and callus induction by radiation mutation of A. membranaceus Bge. [ Method ] With the different parts of Astragalus membranaceus Bge. var. monghol...[Objective] The research aimed to study the tissue culture technology and callus induction by radiation mutation of A. membranaceus Bge. [ Method ] With the different parts of Astragalus membranaceus Bge. var. mongholicus （ Bge. ） Hsiao aseptic seedling as explants （ leaves, cotyledons, hypocotyls） induced callus, and cotyledon and hypocotyls taken by the method of radiation mutation were studied. [ Result]The results showed that the three explants had relatively high callus induced rate in the medium which respectively made up of MS ＋6-BA 2.0 mg/L ＋ NAA2.0 mg/L, LS ＋6-BA2.0 mg/L ＋NAA0.1 mg/L, MS ＋ 6-BA2.0 rng/L ＋ NAA2.0 rag/L; the optimum mutation time of hypocotyls and cotyledons was 15 minutes; the growth of the callus induced from hypocotyls would be better as the mutation time increased, but when it reached a certain time the growth would be weaken, the induction rate also would be reduced. [ Conclusion] This study will provide the scientific reference in tissue culture and mutation breeding of A. membranaceus Bge.展开更多
Amino acid composition of Astragalus Membranaceus Bge(AMB)was analysed by automatic analyzer.The results showed that the amount of combined amino acids was 8.05%of total weight,the free amino acids was 0.38%and the wh...Amino acid composition of Astragalus Membranaceus Bge(AMB)was analysed by automatic analyzer.The results showed that the amount of combined amino acids was 8.05%of total weight,the free amino acids was 0.38%and the whole amino acids was 8.43%.AMB contains all of the essential amino acids except Trp xysshhant.Besides the therapy effect,the AMB has significant nutrient effect.展开更多
[Objectives] The content of astragaloside in Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hisao from three different regions was determined.[Methods] Referring to the method recorded in the Chinese Pharma...[Objectives] The content of astragaloside in Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hisao from three different regions was determined.[Methods] Referring to the method recorded in the Chinese Pharmacopoeia(2015 edition),the content of astragaloside IV in A.membranaceus was determined by HPLC.[Results] There were great differences in the astragaloside IV content of A.membranaceus among different regions.The content of astragaloside IV in A.membranaceus cultivated in Inner Mongolia was highest(0.155%),followed by that(0.143%) in A.membranaceus cultivated in Gansu,and the content of astragaloside IV in A.membranaceus cultivated in Shanxi was lowest(0.080%).The contents of astragaloside IV in A.membranaceus from different regions were all in line with the standard(not less than 0.040%) of Chinese Pharmacopoeia(2015 edition).[Conclusions]The content of astragaloside IV in A.membranaceus cultivated in three different regions met the medicinal standards.展开更多
Objective:To investigate the effect and the mechanism of Astragalus membranaceus(Huangqi in Chinese,HQ)extract on the intestinal absorption of six alkaloids of Aconitum carmichaelii(Fuzi in Chinese,FZ)in rats with spl...Objective:To investigate the effect and the mechanism of Astragalus membranaceus(Huangqi in Chinese,HQ)extract on the intestinal absorption of six alkaloids of Aconitum carmichaelii(Fuzi in Chinese,FZ)in rats with spleen deficiency and provide novel insights into the application of HQ on modulating intestinal barrier.Methods:Four-week-old male Sprague-Dawley rats were fed with Xiaochengqi Decoction to induce the spleen deficiency model for 40 d.Single-pass intestinal perfusion model were used to study the effects of HQ extract on the absorption of alkaloids.Protein expression and mRNA levels of MRP2 and BCRP and tight junction proteins(TJ,including Claudin-1,Occludin and ZO-1)were measured using Western blot and real-time PCR,respectively.The location and expression of TJ protein was also investigated by the immunofluorescence method.Results:Compared with the normal group,the protein expression of MRP2,BCRP and TJ proteins in the model group were significantly down-regulated.After oral administration of HQ,the alkaloid absorption in intestinal villi was inhibited,MRP2,BCRP and TJ proteins were up-regulated,the green fluorescence staining of Claudin-1,Occludin,and ZO-1 was enhanced,and a thick layer of mucus was deposited on the surface of the epithelium of the intestinal cavity.Conclusion:HQ as an intestinal barrier modulator improves the physiological changes of the intestinal environment of spleen deficiency to reduce the absorption of toxic components,leading to a decrease in the absorption of drug-like molecules.展开更多
文摘[Objective] The research aimed to study the tissue culture technology and callus induction by radiation mutation of A. membranaceus Bge. [ Method ] With the different parts of Astragalus membranaceus Bge. var. mongholicus （ Bge. ） Hsiao aseptic seedling as explants （ leaves, cotyledons, hypocotyls） induced callus, and cotyledon and hypocotyls taken by the method of radiation mutation were studied. [ Result]The results showed that the three explants had relatively high callus induced rate in the medium which respectively made up of MS ＋6-BA 2.0 mg/L ＋ NAA2.0 mg/L, LS ＋6-BA2.0 mg/L ＋NAA0.1 mg/L, MS ＋ 6-BA2.0 rng/L ＋ NAA2.0 rag/L; the optimum mutation time of hypocotyls and cotyledons was 15 minutes; the growth of the callus induced from hypocotyls would be better as the mutation time increased, but when it reached a certain time the growth would be weaken, the induction rate also would be reduced. [ Conclusion] This study will provide the scientific reference in tissue culture and mutation breeding of A. membranaceus Bge.
文摘Amino acid composition of Astragalus Membranaceus Bge(AMB)was analysed by automatic analyzer.The results showed that the amount of combined amino acids was 8.05%of total weight,the free amino acids was 0.38%and the whole amino acids was 8.43%.AMB contains all of the essential amino acids except Trp xysshhant.Besides the therapy effect,the AMB has significant nutrient effect.
基金Supported by Tianjin Natural Science Fund(17JCYBJC29800)Tianjin Agricultural College Various Talents Funding Plan Project(J01009030702)+1 种基金Science and Technology Project in the Field of Social Development of Binhai New Area,TianjinAgricultural Science and Technology Plan Project of Baodi District,Tianjin(201838)
文摘[Objectives] The content of astragaloside in Astragalus membranaceus(Fisch.) Bge.var.mongholicus(Bge.) Hisao from three different regions was determined.[Methods] Referring to the method recorded in the Chinese Pharmacopoeia(2015 edition),the content of astragaloside IV in A.membranaceus was determined by HPLC.[Results] There were great differences in the astragaloside IV content of A.membranaceus among different regions.The content of astragaloside IV in A.membranaceus cultivated in Inner Mongolia was highest(0.155%),followed by that(0.143%) in A.membranaceus cultivated in Gansu,and the content of astragaloside IV in A.membranaceus cultivated in Shanxi was lowest(0.080%).The contents of astragaloside IV in A.membranaceus from different regions were all in line with the standard(not less than 0.040%) of Chinese Pharmacopoeia(2015 edition).[Conclusions]The content of astragaloside IV in A.membranaceus cultivated in three different regions met the medicinal standards.
基金supported by a grant from the Key Laboratory of Modern Preparation of TCM of Ministry of Education,Jiangxi University of TCM and Affiliated Hospital,Jiujiang UniversityScience Foundation of Health and Family Planning Commission of Jiangxi Province (Grant No.20181140)+2 种基金National Natural Science Foundation of China (Grant No.81660757)Project of Jiangxi Education Department (Grant No.170732Grant No.201819)
文摘Objective:To investigate the effect and the mechanism of Astragalus membranaceus(Huangqi in Chinese,HQ)extract on the intestinal absorption of six alkaloids of Aconitum carmichaelii(Fuzi in Chinese,FZ)in rats with spleen deficiency and provide novel insights into the application of HQ on modulating intestinal barrier.Methods:Four-week-old male Sprague-Dawley rats were fed with Xiaochengqi Decoction to induce the spleen deficiency model for 40 d.Single-pass intestinal perfusion model were used to study the effects of HQ extract on the absorption of alkaloids.Protein expression and mRNA levels of MRP2 and BCRP and tight junction proteins(TJ,including Claudin-1,Occludin and ZO-1)were measured using Western blot and real-time PCR,respectively.The location and expression of TJ protein was also investigated by the immunofluorescence method.Results:Compared with the normal group,the protein expression of MRP2,BCRP and TJ proteins in the model group were significantly down-regulated.After oral administration of HQ,the alkaloid absorption in intestinal villi was inhibited,MRP2,BCRP and TJ proteins were up-regulated,the green fluorescence staining of Claudin-1,Occludin,and ZO-1 was enhanced,and a thick layer of mucus was deposited on the surface of the epithelium of the intestinal cavity.Conclusion:HQ as an intestinal barrier modulator improves the physiological changes of the intestinal environment of spleen deficiency to reduce the absorption of toxic components,leading to a decrease in the absorption of drug-like molecules.
