OBJECTIVE: To investigate the therapeutic effect of Astragali on sodium and water retention in aortocaval fistula-caused experimental congestive heart failure and its involved mechanisms. METHODS: In aortocaval fistul...OBJECTIVE: To investigate the therapeutic effect of Astragali on sodium and water retention in aortocaval fistula-caused experimental congestive heart failure and its involved mechanisms. METHODS: In aortocaval fistula-caused chronic (5 wk), heart failure rats treated with and without Astragali 1.0 g/day intraperitoneally, changes of cardiac and renal function, renal response to atrial natriuretic peptide (ANP) were examined. Dot blot analysis was used to determine the effect of Astragali on hypothalamic arginine vasopresin (AVP) mRNA expression, and mRNA expressions of aortic and renal AVP V1a receptor, renal AVP V2 receptor and aquaporin-2 (AQP2) were simultaneously detected by RT-PCR method. RESULTS: Rats with aortocaval fistula impaired cardiac and renal functions evidenced by higher right atrial pressure (RAP), left ventricular end-diastolic pressure (LVEDP), lower + dP/dtmax of left ventricle, glomerular filtration rate (GFR), renal plasma flow (RPF), urine volume (UV), urinary sodium excretion (UNaV) and free water clearance (CH2O) compared with sham-operated control (P展开更多
Objective To evaluate the effect of astragalus membranaceus (AM) in combination with taurine on coxasckievirus B3 (CVB3) murine myocarditis. Methods One hundred and sixty mice were infected with CVB3 and equally di...Objective To evaluate the effect of astragalus membranaceus (AM) in combination with taurine on coxasckievirus B3 (CVB3) murine myocarditis. Methods One hundred and sixty mice were infected with CVB3 and equally divided into four groups: normal saline (NS) treated group, AM treated group, taurine treated group and AM combined with taurine treated group. The treatment were carried out from day 1 to 7 after infection. Ten mice were extracted from each group for observing mortality within day 21 postinfection, the rests were killed and the hearts were removed on day 8 (n=15) and 21 (all survival mice), respectively. Electrocardiograph (ECG) of each mouse was analysed before infection and repeated on the day of being killed. CVB3 RNA in murine myocardium was detected by in situ hybridization and myocardial lesions were observed by light microscope. Results Mortality was lower in drug treated groups than that in NS treated group, and it was the lowest in AM combined with taurine treated group, but all the differences were not significant (P>0.05). All drug treatments reduced the incidence of abnormal ECG changes in the acute phase of infection (P<0.05, vs NS treated group), but AM combined with taurine was the most effective in reducing the incidence of abnormal ECG (P<0.05, vs other groups). AM inhibited viral replication in myocardium at the early and late stage of murine myocarditis effectively and AM combined with taurine enhanced the effect of anti virus. All drugs decreased myocardial damage (P<0.05, vs control group), and the effect of AM combined with taurine was most significant (P<0.05). Conculsion The AM combined with taurine on CVB3 murine myocarditis is more effective than that of AM or taurine.展开更多
为优化黄芪瞬时高温灭菌(high temperature short time,HTST)工艺参数并多维度考察其对黄芪质量的影响,基于指标相关性的权重系数(criteria importance though intercrieria correlation,CRITIC)法采用正交设计优化黄芪灭菌工艺参数,以...为优化黄芪瞬时高温灭菌(high temperature short time,HTST)工艺参数并多维度考察其对黄芪质量的影响,基于指标相关性的权重系数(criteria importance though intercrieria correlation,CRITIC)法采用正交设计优化黄芪灭菌工艺参数,以灭菌温度、灭菌时间、药材粉碎粒度为考察因素,以灭菌率、5种化合物含量及1,1-二苯基-2-苦基肼自由基(1,1-diphenyl-2-picrylhydrazyl,DPPH·)清除率为考察指标,通过直观及方差分析评价灭菌对3个考察指标的影响;并运用液质联用技术(liquid chromatography-mass spectrometry,LC-MS)确认指纹图谱共有峰的结构,以偏最小二乘回归分析法分析共有峰与抗氧化活性的谱效关系。结果显示,灭菌温度是具有显著影响的因素(P<0.05),最佳灭菌工艺参数为灭菌温度(170±2)℃,灭菌时间5 s,粉碎粒度50目;按优化工艺灭菌后的3批样品微生物水平均符合药典规定,5种化合物含量及DPPH·清除率与灭菌前比较无明显变化;灭菌前后指纹图谱相似度均大于0.900;谱效学分析相关性结果与化合物单体抗氧化的试验结果及其结构特点基本一致。综上所述,瞬时高温灭菌对黄芪中微生物具有明显杀灭作用且对其质量无明显影响,表明该方法适用于黄芪药材灭菌。展开更多
文摘OBJECTIVE: To investigate the therapeutic effect of Astragali on sodium and water retention in aortocaval fistula-caused experimental congestive heart failure and its involved mechanisms. METHODS: In aortocaval fistula-caused chronic (5 wk), heart failure rats treated with and without Astragali 1.0 g/day intraperitoneally, changes of cardiac and renal function, renal response to atrial natriuretic peptide (ANP) were examined. Dot blot analysis was used to determine the effect of Astragali on hypothalamic arginine vasopresin (AVP) mRNA expression, and mRNA expressions of aortic and renal AVP V1a receptor, renal AVP V2 receptor and aquaporin-2 (AQP2) were simultaneously detected by RT-PCR method. RESULTS: Rats with aortocaval fistula impaired cardiac and renal functions evidenced by higher right atrial pressure (RAP), left ventricular end-diastolic pressure (LVEDP), lower + dP/dtmax of left ventricle, glomerular filtration rate (GFR), renal plasma flow (RPF), urine volume (UV), urinary sodium excretion (UNaV) and free water clearance (CH2O) compared with sham-operated control (P
文摘Objective To evaluate the effect of astragalus membranaceus (AM) in combination with taurine on coxasckievirus B3 (CVB3) murine myocarditis. Methods One hundred and sixty mice were infected with CVB3 and equally divided into four groups: normal saline (NS) treated group, AM treated group, taurine treated group and AM combined with taurine treated group. The treatment were carried out from day 1 to 7 after infection. Ten mice were extracted from each group for observing mortality within day 21 postinfection, the rests were killed and the hearts were removed on day 8 (n=15) and 21 (all survival mice), respectively. Electrocardiograph (ECG) of each mouse was analysed before infection and repeated on the day of being killed. CVB3 RNA in murine myocardium was detected by in situ hybridization and myocardial lesions were observed by light microscope. Results Mortality was lower in drug treated groups than that in NS treated group, and it was the lowest in AM combined with taurine treated group, but all the differences were not significant (P>0.05). All drug treatments reduced the incidence of abnormal ECG changes in the acute phase of infection (P<0.05, vs NS treated group), but AM combined with taurine was the most effective in reducing the incidence of abnormal ECG (P<0.05, vs other groups). AM inhibited viral replication in myocardium at the early and late stage of murine myocarditis effectively and AM combined with taurine enhanced the effect of anti virus. All drugs decreased myocardial damage (P<0.05, vs control group), and the effect of AM combined with taurine was most significant (P<0.05). Conculsion The AM combined with taurine on CVB3 murine myocarditis is more effective than that of AM or taurine.
文摘为优化黄芪瞬时高温灭菌(high temperature short time,HTST)工艺参数并多维度考察其对黄芪质量的影响,基于指标相关性的权重系数(criteria importance though intercrieria correlation,CRITIC)法采用正交设计优化黄芪灭菌工艺参数,以灭菌温度、灭菌时间、药材粉碎粒度为考察因素,以灭菌率、5种化合物含量及1,1-二苯基-2-苦基肼自由基(1,1-diphenyl-2-picrylhydrazyl,DPPH·)清除率为考察指标,通过直观及方差分析评价灭菌对3个考察指标的影响;并运用液质联用技术(liquid chromatography-mass spectrometry,LC-MS)确认指纹图谱共有峰的结构,以偏最小二乘回归分析法分析共有峰与抗氧化活性的谱效关系。结果显示,灭菌温度是具有显著影响的因素(P<0.05),最佳灭菌工艺参数为灭菌温度(170±2)℃,灭菌时间5 s,粉碎粒度50目;按优化工艺灭菌后的3批样品微生物水平均符合药典规定,5种化合物含量及DPPH·清除率与灭菌前比较无明显变化;灭菌前后指纹图谱相似度均大于0.900;谱效学分析相关性结果与化合物单体抗氧化的试验结果及其结构特点基本一致。综上所述,瞬时高温灭菌对黄芪中微生物具有明显杀灭作用且对其质量无明显影响,表明该方法适用于黄芪药材灭菌。
