Genetic relationships among 22 accessions of Astragalus sinicus L. col ect-ed from different provinces of China were analyzed by inter-simple sequence repeat (ISSR) markers. The results showed that 10 highly reprodu...Genetic relationships among 22 accessions of Astragalus sinicus L. col ect-ed from different provinces of China were analyzed by inter-simple sequence repeat (ISSR) markers. The results showed that 10 highly reproducible ISSR fragments a-mong 40 primers were screened. Using these primers, a total of 684 ISSR frag-ments from 500 to 3 000 bp were amplified, and 59.2% of them showed polymor-phic by unweighted pair-group method with arithmetic (UPGMA) analysis. It revealed that the 22 accessions had a similarity range from 0.63 to 0.95, and existed biolog-ical diversities. Based on cluster and principal coordinate analyses, al accessions could be divided into four distinct groups.展开更多
基金Supported by the Public Benefit Research Foundation of National Departments,China(201103005)~~
文摘Genetic relationships among 22 accessions of Astragalus sinicus L. col ect-ed from different provinces of China were analyzed by inter-simple sequence repeat (ISSR) markers. The results showed that 10 highly reproducible ISSR fragments a-mong 40 primers were screened. Using these primers, a total of 684 ISSR frag-ments from 500 to 3 000 bp were amplified, and 59.2% of them showed polymor-phic by unweighted pair-group method with arithmetic (UPGMA) analysis. It revealed that the 22 accessions had a similarity range from 0.63 to 0.95, and existed biolog-ical diversities. Based on cluster and principal coordinate analyses, al accessions could be divided into four distinct groups.