A sensitive and selective method based on gas chromatography hyphenated to mass spectrometry （GC- MS） was developed and validated for the determination of atractylon in rat plasma. Plasma samples were processed by l...A sensitive and selective method based on gas chromatography hyphenated to mass spectrometry （GC- MS） was developed and validated for the determination of atractylon in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate-n-hexane （1：1, v/v） using acetophenone as an internal standard （IS）. Analytes were determined in selective ion monitoring （SIM） mode using target ions at m/z 108.1 for atractylon and m/z 105.1 for acetophenone. The calibration curve was linear over the concentration range of 10-1000 ng/mL with lower limit of quantification of 10 ng/mL. The intra- and inter-day precision variations were not more than 10.4% and 9.6%, respectively, whilst accuracy values ranged from -6.5% to 4.9%. Extraction recovery of the assay was satisfactory. This method was suc- cessfully applied to quantification and pharmacokinetic study of atractylon in rat plasma after in- tragastric administration of Atractvlodis extract.展开更多
基金supported by the National Natural Science Foundation of China(Nos.81073143 and 81373926)Research Fund for the Doctoral Program of Higher Education of China(No. 20092134110004)
文摘A sensitive and selective method based on gas chromatography hyphenated to mass spectrometry （GC- MS） was developed and validated for the determination of atractylon in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate-n-hexane （1：1, v/v） using acetophenone as an internal standard （IS）. Analytes were determined in selective ion monitoring （SIM） mode using target ions at m/z 108.1 for atractylon and m/z 105.1 for acetophenone. The calibration curve was linear over the concentration range of 10-1000 ng/mL with lower limit of quantification of 10 ng/mL. The intra- and inter-day precision variations were not more than 10.4% and 9.6%, respectively, whilst accuracy values ranged from -6.5% to 4.9%. Extraction recovery of the assay was satisfactory. This method was suc- cessfully applied to quantification and pharmacokinetic study of atractylon in rat plasma after in- tragastric administration of Atractvlodis extract.
