In this paper, double hydrophilic ionic liquid block copolymers （ILBCs）, poly poly[1-methyl-3-（2-methacryloyloxy propylimidazolium bromine）]-block-（N.isopro. pylacrylamide） （PMMPImB-b-PNIPAAm） was first synthe...In this paper, double hydrophilic ionic liquid block copolymers （ILBCs）, poly poly[1-methyl-3-（2-methacryloyloxy propylimidazolium bromine）]-block-（N.isopro. pylacrylamide） （PMMPImB-b-PNIPAAm） was first synthesized by reversible addition- fragmentation chain transfer （RAFT） and then attached on the surface of gold nanoparticles （Au NPs） via a strong gold-sulfur bonding for preparing hybrid nanoparticles （PMMPImB-b-PNIPAAm-@-Au NPs）. The hybrid NPs had a three layers micelle-like structure, including a gold core, thermo-responsive inner shell and anion responsive outer corona. The self-assembling behavior of thermal- and anion-response from shell and corona were respectively investigated by change of temperature and addition of （CF3SO2）2N-. The results showed the hybrid NPs retained a stable dispersion beyond the lower critical solution temperature （LCST） because of the space or electrostatic protecting by outer PMMPImBo However, with increasing concentration of （CF3SO2）2N-, the micellization of self-assembling PMMPImB-b-PNIPAAm-@-Au NPs was induced to form micellar structure containing the core with hydrophobic PMMPImB- （CF3SO2）2N- surrounded by composite shell of Au NPs-PNIPAAm via the anion- responsive properties of ILBCs. These results indicated that the block copolymers protected plasmonic nanoparticles remain self-assembling properties of block copoly- mers when phase transition from outer corona polymer.展开更多
Based on MEMS technology,immunosensor with an'Au,Pt,Pt'three-microelectrode system enclosed in a SU-8 micro pool was fabricated.Employing SAMs technique,the Au electrode was modified by cysteamine(Cys)to assem...Based on MEMS technology,immunosensor with an'Au,Pt,Pt'three-microelectrode system enclosed in a SU-8 micro pool was fabricated.Employing SAMs technique,the Au electrode was modified by cysteamine(Cys)to assemble gold nanopanicles(nanogold)layer,subsequently,a layer of protein G(PG)was immobilized on nanogold layer to further capture antibody orientedly.Compared with the immunosensors using bulky gold electrode and direct PG binding to electrode immobilization technique for antibody,it has attractive advantages,such as miniaturization,good compatibility,broad linear range for human immunoglobulin(HIgG)and easy to be designed into array.展开更多
Bimetallic Au/Pd nanoparticles were prepared and used to catalyze oxidation of alcohols in the poly(ethylene glycol) (PEG)/CO_(2) biphasic system using O2 as the oxidant without adding any base.The catalytic activity ...Bimetallic Au/Pd nanoparticles were prepared and used to catalyze oxidation of alcohols in the poly(ethylene glycol) (PEG)/CO_(2) biphasic system using O2 as the oxidant without adding any base.The catalytic activity of Au/Pd bimetal with different mole ratios was studied using benzyl alcohol as the substrate.It was found that bimetallic Au/Pd nanoparticles with Au:Pd=1:3.5 had higher catalytic activity than monometallic Au,Pd and the bimetallic Au/Pd nanoparticles with other molar ratios.The effect of CO_(2) pressure on the oxidation of benzyl alcohol and 1-phenylethanol in PEG/CO_(2) was investigated.It was demonstrated that CO_(2) pressure could be used to tune the conversion and selectivity of the reactions effectively.α,β-Unsaturated alcohols were also studied and found to be more reactive than benzyl alcohol and 1-phenylethanol.Recycling experiments showed that the Au/Pd/PEG/CO_(2) catalytic system could be recycled at least four times without reducing the activity.In addition,the catalytic system is clean and the products can be separated easily.展开更多
To explore the effects of microenvironmental adjustments on fluorescence,a pH-sensitive nanocomposite system based on fluorescence resonance energy transfer(FRET)was constructed.The model system included a modified tr...To explore the effects of microenvironmental adjustments on fluorescence,a pH-sensitive nanocomposite system based on fluorescence resonance energy transfer(FRET)was constructed.The model system included a modified triblock copolymer(polyhistidine-b-polyethylene glycol-b-polycaprolactone)and gold nanoparticles.A near-infrared dye was used as the donor,and spectrally matched gold nanorods,attached after C-terminus modification with α-lipoic acid,were used as the receptor to realize control of the FRET effect over the fluorescence intensity for two polymer configurational changes(i.e.,"folded"and"stretched"states)in response to pH.After synthesis and characterization,we investigated the self-assembly behavior of the system.Analysis by quartz crystal microbalance revealed the pH sensitivity of the polymer,which exhibited"folding"and"stretching"states with changes in pH,providing a structural basis for the FRET effect.Fluorescence spectrophotometry investigations also revealed the regulatory impact of the assembled system on fluorescence.展开更多
文摘In this paper, double hydrophilic ionic liquid block copolymers （ILBCs）, poly poly[1-methyl-3-（2-methacryloyloxy propylimidazolium bromine）]-block-（N.isopro. pylacrylamide） （PMMPImB-b-PNIPAAm） was first synthesized by reversible addition- fragmentation chain transfer （RAFT） and then attached on the surface of gold nanoparticles （Au NPs） via a strong gold-sulfur bonding for preparing hybrid nanoparticles （PMMPImB-b-PNIPAAm-@-Au NPs）. The hybrid NPs had a three layers micelle-like structure, including a gold core, thermo-responsive inner shell and anion responsive outer corona. The self-assembling behavior of thermal- and anion-response from shell and corona were respectively investigated by change of temperature and addition of （CF3SO2）2N-. The results showed the hybrid NPs retained a stable dispersion beyond the lower critical solution temperature （LCST） because of the space or electrostatic protecting by outer PMMPImBo However, with increasing concentration of （CF3SO2）2N-, the micellization of self-assembling PMMPImB-b-PNIPAAm-@-Au NPs was induced to form micellar structure containing the core with hydrophobic PMMPImB- （CF3SO2）2N- surrounded by composite shell of Au NPs-PNIPAAm via the anion- responsive properties of ILBCs. These results indicated that the block copolymers protected plasmonic nanoparticles remain self-assembling properties of block copoly- mers when phase transition from outer corona polymer.
基金This work is supported by the National Natural Science Foundation of China （Grant No. 90307014）.
文摘Based on MEMS technology,immunosensor with an'Au,Pt,Pt'three-microelectrode system enclosed in a SU-8 micro pool was fabricated.Employing SAMs technique,the Au electrode was modified by cysteamine(Cys)to assemble gold nanopanicles(nanogold)layer,subsequently,a layer of protein G(PG)was immobilized on nanogold layer to further capture antibody orientedly.Compared with the immunosensors using bulky gold electrode and direct PG binding to electrode immobilization technique for antibody,it has attractive advantages,such as miniaturization,good compatibility,broad linear range for human immunoglobulin(HIgG)and easy to be designed into array.
基金the National Natural Science Foundation of China (20973177 & 20932002)Ministry of Science and Technology of China (2006CB202504)Chinese Academy of Sciences (KJCX2. YW.H16) for financial supports
文摘Bimetallic Au/Pd nanoparticles were prepared and used to catalyze oxidation of alcohols in the poly(ethylene glycol) (PEG)/CO_(2) biphasic system using O2 as the oxidant without adding any base.The catalytic activity of Au/Pd bimetal with different mole ratios was studied using benzyl alcohol as the substrate.It was found that bimetallic Au/Pd nanoparticles with Au:Pd=1:3.5 had higher catalytic activity than monometallic Au,Pd and the bimetallic Au/Pd nanoparticles with other molar ratios.The effect of CO_(2) pressure on the oxidation of benzyl alcohol and 1-phenylethanol in PEG/CO_(2) was investigated.It was demonstrated that CO_(2) pressure could be used to tune the conversion and selectivity of the reactions effectively.α,β-Unsaturated alcohols were also studied and found to be more reactive than benzyl alcohol and 1-phenylethanol.Recycling experiments showed that the Au/Pd/PEG/CO_(2) catalytic system could be recycled at least four times without reducing the activity.In addition,the catalytic system is clean and the products can be separated easily.
基金the funding and generous support of Zhejiang Province Natural Science Foundation(Nos.LY17C100003,Y17E030032)Key Research Project of Traditional Chinese Medicine of Zhejiang Province of China(No.2019ZZ015)Medical Health Science and Technology Project of Zhejiang Provincial Health Commission(No.2018KY131)。
文摘To explore the effects of microenvironmental adjustments on fluorescence,a pH-sensitive nanocomposite system based on fluorescence resonance energy transfer(FRET)was constructed.The model system included a modified triblock copolymer(polyhistidine-b-polyethylene glycol-b-polycaprolactone)and gold nanoparticles.A near-infrared dye was used as the donor,and spectrally matched gold nanorods,attached after C-terminus modification with α-lipoic acid,were used as the receptor to realize control of the FRET effect over the fluorescence intensity for two polymer configurational changes(i.e.,"folded"and"stretched"states)in response to pH.After synthesis and characterization,we investigated the self-assembly behavior of the system.Analysis by quartz crystal microbalance revealed the pH sensitivity of the polymer,which exhibited"folding"and"stretching"states with changes in pH,providing a structural basis for the FRET effect.Fluorescence spectrophotometry investigations also revealed the regulatory impact of the assembled system on fluorescence.
