Au is considered as one of the most promising catalysts for nitrogen reduction reaction(NRR),however maximizing the activity utilization rate of Au and understanding the synergistic effects between Au and carriers pos...Au is considered as one of the most promising catalysts for nitrogen reduction reaction(NRR),however maximizing the activity utilization rate of Au and understanding the synergistic effects between Au and carriers pose ongoing challenges.Herein,we systematically explore the synergistic catalytic effect of incorporating Au with boron clusters for accelerating NRR kinetics.An in-situ abinitio strategy is employed to construct B-doped Au nanoparticles(2-6 nm in diameter)loaded on BO_(x) substrates(AuBO_(x)),in which B not only modulates the surface electronic structure of Au but also forms strong coupling interactions to stabilize the nanoparticles.The electrochemical results show that Au-BO_(x) possesses excellent NRR activity(NH_(3) yield of 48.52μg h^(-1)mg_(cat)^(-1),Faraday efficiency of 56.18%),and exhibits high stability and reproducibility throughout the electrocatalytic NRR process.Theoretical calculations reveal that the introduction of B induces the formation of both Au dangling bond and Au-B coupling bond.which considerably facilitates the hydrogenation of~*N_(2)^(-)~*NH_(3).The present work provides a new avenue for the preparation of metal-boron materials achieved by one-step reduction and doping process,utilizing boron clusters as reducing and stabilizing agents.展开更多
Gold（Au） nanoparticle HBV DNA or HCV cDNA gene probes were prepared and were used to detect HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection directly by transmission electro...Gold（Au） nanoparticle HBV DNA or HCV cDNA gene probes were prepared and were used to detect HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection directly by transmission electron microscopy （TEM）. PCR identifying HBV and HCV in serum of patients with HBV and HCV coinfection was established. Alkanethiol-modified oligonucleotide was bound with self-made Au nanoparticles to form nanoparticle HBV DNA or HCV cDNA gene probes through covalent binding of Au-S. HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection was added to the detection system composed of nanoparticle HBV DNA and（or） HCV cDNA gene probes, The results showed that HBV DNA and HCV RNA could be specifically amplified by PCR. The zones of DNA amplification appeared in 431 bp and 323 bp respectively. When HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection were added to the detection system, TEM displayed the nanoparticles self-assembled into large network aggregates. It was concluded that the detection of HBV and HCV coinfection by TEM was convenient and efficient with high specificity and sensitivity.展开更多
The Liaoji Proterozoic rift is an inter-intracontinenatl rift developed from Archean granite-greenstone tectonic regime and contains many important mineral deposits of U, B, magnesite, Pb-Zn, Au, Ag, Co and P. These d...The Liaoji Proterozoic rift is an inter-intracontinenatl rift developed from Archean granite-greenstone tectonic regime and contains many important mineral deposits of U, B, magnesite, Pb-Zn, Au, Ag, Co and P. These deposits were formed as the result of late mobilization, transportation and concentfation of the previously enriched ore-forming mate- rials in several ore-bearing formations formed during the rift stage. So the metallogeny of these deposits in the rift shows both inheritance and new generation of the ore-forming materials. In future ore-searching practice, attentions should be paid on the studies of the ore-bearing formations in the rift, on the multiple stages of metallogeny and and on multiple derivations of the ore-forming materials.展开更多
Objective：Gold nanoparticle Hepatitis B virus （HBV） DNA probes were prepared, and their application for HBV DNA measurement was studied. Methods：Alkanethiol modified oligonucleotide was bound with self-made Au nan...Objective：Gold nanoparticle Hepatitis B virus （HBV） DNA probes were prepared, and their application for HBV DNA measurement was studied. Methods：Alkanethiol modified oligonucleotide was bound with self-made Au nanoparticles to form nanoparticle HBV DNA gene probes, through covalent binding of Au-S. By using a fluorescence-based method, the number of thiol-derivatized, single-stranded oligonucleotides and their hybridization efficiency with complementary oligonucleotides in solution was determined. With the aid of Au nanoparticle-supported mercapto-modified oligonucleotides serving as detection probes, and oligonucleotides immobilized on a nylon membrane surface acting as capturing probes, HBV DNA was detected visually by sandwich hybridization based on highly sensitive aggregation and silver staining. The modified nanoparticle HBV DNA gene probes were also used to detect the HBV DNA extracted from serum in patients with hepatitis B. Results：Compared with bare Au nanoparticles, oligonucleotide modified nanoparticles had a higher stability in NaCl solution or under high temperature environment and the absorbance peak of modified Au nanoparticles shifted from 520nm to 524nm. For Au nanoparticles, the maximal oligonucleotide surface coverage of hexaethiol 30-mer oligonucleotide was （132 ± 10） oligonucleotides per nanoparticle, and the percentage of hybridization strands on nanoparticles was （22 ± 3% ）. Based on a two-probe sandwich hybridization/nanoparticle amplification/silver staining enhancement method, Au nanoparticle gene probes could detect as low as 10^-11 mol/L composite HBV DNA molecules on a nylon membrane and the PCR products of HBV DNA visually. As made evident by transmission electron microscopy, the nanoparticles assembled into large network aggregates when nanoparticle HBV DNA gene probes were applied to detect HBV DNA molecules in liquid. Conclusion：Our results showed that successfully prepared Au nanoparticle HBV DNA gene probes could be used to detect HBV DNA directly. The detection-visuallized method has many advantages, including high sensitivity, simple operation and low cost. This technique has potential applications in many fields, especially in multi-gene detection chips.展开更多
本研究探讨多壁碳纳米管为载体组装金粒子及乙脑疫苗抗体,制备无电子媒介体乙脑疫苗免疫传感器的可行性及其基本性能。采用恒电位法在碳纳米管表面沉积金微粒,再利用物理吸附在金微粒表面固定乙脑疫苗抗体,并通过辣根过氧化氢酶封闭传...本研究探讨多壁碳纳米管为载体组装金粒子及乙脑疫苗抗体,制备无电子媒介体乙脑疫苗免疫传感器的可行性及其基本性能。采用恒电位法在碳纳米管表面沉积金微粒,再利用物理吸附在金微粒表面固定乙脑疫苗抗体,并通过辣根过氧化氢酶封闭传感器上的非特异性活性位点,利用其对H_2O_2的反应增强电流信号,制得乙脑免疫传感器。循环伏安法被用来对制备各阶段电极进行表征,多种电化学方法详细研究了免疫传感器性能。实验结果表明该免疫传感器在5.6×10^(-8)～2.2×10^(-6) lg pfu/mL的范围内保持良好的线性关系,具有较高的灵敏度(72.4μA/lg pfu/mL/cm^2),较好的稳定性和选择性,具有潜在应用价值。展开更多
基金supported by the National Natural Science Foundation of China(22075133,62288102,22375091,21971114,and 21701086)the Jiangsu Provincial Funds(BX2022013)。
文摘Au is considered as one of the most promising catalysts for nitrogen reduction reaction(NRR),however maximizing the activity utilization rate of Au and understanding the synergistic effects between Au and carriers pose ongoing challenges.Herein,we systematically explore the synergistic catalytic effect of incorporating Au with boron clusters for accelerating NRR kinetics.An in-situ abinitio strategy is employed to construct B-doped Au nanoparticles(2-6 nm in diameter)loaded on BO_(x) substrates(AuBO_(x)),in which B not only modulates the surface electronic structure of Au but also forms strong coupling interactions to stabilize the nanoparticles.The electrochemical results show that Au-BO_(x) possesses excellent NRR activity(NH_(3) yield of 48.52μg h^(-1)mg_(cat)^(-1),Faraday efficiency of 56.18%),and exhibits high stability and reproducibility throughout the electrocatalytic NRR process.Theoretical calculations reveal that the introduction of B induces the formation of both Au dangling bond and Au-B coupling bond.which considerably facilitates the hydrogenation of~*N_(2)^(-)~*NH_(3).The present work provides a new avenue for the preparation of metal-boron materials achieved by one-step reduction and doping process,utilizing boron clusters as reducing and stabilizing agents.
基金This project was supported by National Key Basic Research Program of China (No 2007CB512900,2005CB 522901) National Science Foundation of China (No NSFC30571643)
文摘Gold（Au） nanoparticle HBV DNA or HCV cDNA gene probes were prepared and were used to detect HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection directly by transmission electron microscopy （TEM）. PCR identifying HBV and HCV in serum of patients with HBV and HCV coinfection was established. Alkanethiol-modified oligonucleotide was bound with self-made Au nanoparticles to form nanoparticle HBV DNA or HCV cDNA gene probes through covalent binding of Au-S. HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection was added to the detection system composed of nanoparticle HBV DNA and（or） HCV cDNA gene probes, The results showed that HBV DNA and HCV RNA could be specifically amplified by PCR. The zones of DNA amplification appeared in 431 bp and 323 bp respectively. When HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection were added to the detection system, TEM displayed the nanoparticles self-assembled into large network aggregates. It was concluded that the detection of HBV and HCV coinfection by TEM was convenient and efficient with high specificity and sensitivity.
文摘The Liaoji Proterozoic rift is an inter-intracontinenatl rift developed from Archean granite-greenstone tectonic regime and contains many important mineral deposits of U, B, magnesite, Pb-Zn, Au, Ag, Co and P. These deposits were formed as the result of late mobilization, transportation and concentfation of the previously enriched ore-forming mate- rials in several ore-bearing formations formed during the rift stage. So the metallogeny of these deposits in the rift shows both inheritance and new generation of the ore-forming materials. In future ore-searching practice, attentions should be paid on the studies of the ore-bearing formations in the rift, on the multiple stages of metallogeny and and on multiple derivations of the ore-forming materials.
基金This project was supported by National High Technology Program( No.2002AA302202)International S&T Cooperation Program of China(2003DF000034)S&T Program of Wuhan(20041003068-05)
文摘Objective：Gold nanoparticle Hepatitis B virus （HBV） DNA probes were prepared, and their application for HBV DNA measurement was studied. Methods：Alkanethiol modified oligonucleotide was bound with self-made Au nanoparticles to form nanoparticle HBV DNA gene probes, through covalent binding of Au-S. By using a fluorescence-based method, the number of thiol-derivatized, single-stranded oligonucleotides and their hybridization efficiency with complementary oligonucleotides in solution was determined. With the aid of Au nanoparticle-supported mercapto-modified oligonucleotides serving as detection probes, and oligonucleotides immobilized on a nylon membrane surface acting as capturing probes, HBV DNA was detected visually by sandwich hybridization based on highly sensitive aggregation and silver staining. The modified nanoparticle HBV DNA gene probes were also used to detect the HBV DNA extracted from serum in patients with hepatitis B. Results：Compared with bare Au nanoparticles, oligonucleotide modified nanoparticles had a higher stability in NaCl solution or under high temperature environment and the absorbance peak of modified Au nanoparticles shifted from 520nm to 524nm. For Au nanoparticles, the maximal oligonucleotide surface coverage of hexaethiol 30-mer oligonucleotide was （132 ± 10） oligonucleotides per nanoparticle, and the percentage of hybridization strands on nanoparticles was （22 ± 3% ）. Based on a two-probe sandwich hybridization/nanoparticle amplification/silver staining enhancement method, Au nanoparticle gene probes could detect as low as 10^-11 mol/L composite HBV DNA molecules on a nylon membrane and the PCR products of HBV DNA visually. As made evident by transmission electron microscopy, the nanoparticles assembled into large network aggregates when nanoparticle HBV DNA gene probes were applied to detect HBV DNA molecules in liquid. Conclusion：Our results showed that successfully prepared Au nanoparticle HBV DNA gene probes could be used to detect HBV DNA directly. The detection-visuallized method has many advantages, including high sensitivity, simple operation and low cost. This technique has potential applications in many fields, especially in multi-gene detection chips.
基金supported by the National Natural Science Foundation of China(No.21505118)the Natural Science Foundation of Jiangsu Province of China(BK 20150438)Postdoctoral Research Funding Program of Jiangsu Province of China(No.1701133C).
文摘本研究探讨多壁碳纳米管为载体组装金粒子及乙脑疫苗抗体,制备无电子媒介体乙脑疫苗免疫传感器的可行性及其基本性能。采用恒电位法在碳纳米管表面沉积金微粒,再利用物理吸附在金微粒表面固定乙脑疫苗抗体,并通过辣根过氧化氢酶封闭传感器上的非特异性活性位点,利用其对H_2O_2的反应增强电流信号,制得乙脑免疫传感器。循环伏安法被用来对制备各阶段电极进行表征,多种电化学方法详细研究了免疫传感器性能。实验结果表明该免疫传感器在5.6×10^(-8)～2.2×10^(-6) lg pfu/mL的范围内保持良好的线性关系,具有较高的灵敏度(72.4μA/lg pfu/mL/cm^2),较好的稳定性和选择性,具有潜在应用价值。
