在金纳米棒(AuNRs)-Ag^+-甲醛(HCHO)体系中,HCHO快速将Ag^+还原为Ag,Ag包裹在AuNRs表面形成Au@AgNRs,改变了AuNRs周围的电介质环境,导致纵向最大吸收波长(LPAB)红移,同时伴随着溶液的颜色发生显著的变化。据此,发展了一种测定HCHO的快...在金纳米棒(AuNRs)-Ag^+-甲醛(HCHO)体系中,HCHO快速将Ag^+还原为Ag,Ag包裹在AuNRs表面形成Au@AgNRs,改变了AuNRs周围的电介质环境,导致纵向最大吸收波长(LPAB)红移,同时伴随着溶液的颜色发生显著的变化。据此,发展了一种测定HCHO的快响应、简便、灵敏、选择性的AuNRs比色传感器。该比色传感器的检出限为6.3×10^(-11)(g m L^(-1)),比表面增强拉曼光谱法低,显示很高的灵敏度;尤其是本比色传感器用于水样品中HCHO的测定,结果与固体基质室温磷光法相吻合,展示较高的实用性。此外,探讨了测定HCHO的机理。展开更多
The content of biothiols in cells is highly associated with the occurrence and development of several diseases.However,due to their active chemical properties,thiol-contained molecules are normally volatile during the...The content of biothiols in cells is highly associated with the occurrence and development of several diseases.However,due to their active chemical properties,thiol-contained molecules are normally volatile during the detection process,rendering precise analysis of intracellular biothiols challenging.In this study,5,5’-dithiobis-(2-nitrobenzoic acid)(DTNB)is covalently modified on the surface of gold nanorods(AuNRs),constructing sensing substrates for in situ Raman imaging analysis of biothiols in cells.Au NRs are able to serve as ideal surface-enhanced Raman scattering substrates,and thus Raman signals of DTNB are greatly amplified by AuNRs.Meanwhile,the disulfide bond of DTNB can be broken by thiols,thereby releasing part of DTNB from the surface of AuNRs.As a result,three kinds of main biothiols are sensitively quantified with DTNB-modified AuNRs according to the variation of Raman signals,and DTNB-modified Au NRs exhibit far better analytical performance than a commercial probe.In addition,the sensing substrates can be readily delivered to cytoplasm with the transmembrane of Au NRs,and are capable of responding to biothiols in cells.Notably,the Raman approach is established by the breaking of chemical bonds rather than the aggregation of substrates,which is more inclined to analyze intracellular biothiols with a desirable spatial resolution.Therefore,fluctuation of biothiols in glioma cells is evidently observed via Raman imaging.Overall,this work provides an alternative strategy for designing Raman sensors to visualize active molecules in cells.展开更多
文摘在金纳米棒(AuNRs)-Ag^+-甲醛(HCHO)体系中,HCHO快速将Ag^+还原为Ag,Ag包裹在AuNRs表面形成Au@AgNRs,改变了AuNRs周围的电介质环境,导致纵向最大吸收波长(LPAB)红移,同时伴随着溶液的颜色发生显著的变化。据此,发展了一种测定HCHO的快响应、简便、灵敏、选择性的AuNRs比色传感器。该比色传感器的检出限为6.3×10^(-11)(g m L^(-1)),比表面增强拉曼光谱法低,显示很高的灵敏度;尤其是本比色传感器用于水样品中HCHO的测定,结果与固体基质室温磷光法相吻合,展示较高的实用性。此外,探讨了测定HCHO的机理。
基金supported by the National Natural Science Foundation of China(22234005,21974070,22222407,22176099)the Natural Science Foundation of Jiangsu Province(BK20192008)the State Key Laboratory of Analytical Chemistry for Life Science(SKLACLS2220)。
文摘The content of biothiols in cells is highly associated with the occurrence and development of several diseases.However,due to their active chemical properties,thiol-contained molecules are normally volatile during the detection process,rendering precise analysis of intracellular biothiols challenging.In this study,5,5’-dithiobis-(2-nitrobenzoic acid)(DTNB)is covalently modified on the surface of gold nanorods(AuNRs),constructing sensing substrates for in situ Raman imaging analysis of biothiols in cells.Au NRs are able to serve as ideal surface-enhanced Raman scattering substrates,and thus Raman signals of DTNB are greatly amplified by AuNRs.Meanwhile,the disulfide bond of DTNB can be broken by thiols,thereby releasing part of DTNB from the surface of AuNRs.As a result,three kinds of main biothiols are sensitively quantified with DTNB-modified AuNRs according to the variation of Raman signals,and DTNB-modified Au NRs exhibit far better analytical performance than a commercial probe.In addition,the sensing substrates can be readily delivered to cytoplasm with the transmembrane of Au NRs,and are capable of responding to biothiols in cells.Notably,the Raman approach is established by the breaking of chemical bonds rather than the aggregation of substrates,which is more inclined to analyze intracellular biothiols with a desirable spatial resolution.Therefore,fluctuation of biothiols in glioma cells is evidently observed via Raman imaging.Overall,this work provides an alternative strategy for designing Raman sensors to visualize active molecules in cells.
