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High repetition granular Co/Pt multilayers with improved perpendicular remanent magnetization for high-density magnetic recording
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作者 李智 张昆 +7 位作者 杜奥 张洪超 陈伟斌 徐宁 郝润润 颜世申 赵巍胜 冷群文 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第2期405-410,共6页
Thanks to the strong perpendicular magnetic anisotropy(PMA), excellent processing compatibility as well as novel spintronic phenomenon, Co/Pt multilayers have been attracting massive attention and widely used in magne... Thanks to the strong perpendicular magnetic anisotropy(PMA), excellent processing compatibility as well as novel spintronic phenomenon, Co/Pt multilayers have been attracting massive attention and widely used in magnetic storage.However, reversed magnetic domains come into being with the increasing layer repetition ‘N’ to reduce magneto-static energy, resulting in the remarkable diminishment of the remanent magnetization(Mr). As a result, the product of Mrand thickness(i.e., the remanent moment-thickness product, Mrt), a key parameter in magnetic recording for reliable data storing and reading, also decreases dramatically. To overcome this issue, we deposit an ultra-thick granular [Co/Pt]80multilayer with a total thickness of 68 nm on granular SiNxbuffer layer. The Mrt value, Mrto saturation magnetization(Ms) ratio as well as out of plane(OOP) coercivity(Hcoop) are high up to 2.97 memu/cm^(2), 67%, and 1940 Oe(1 Oe = 79.5775 A·m^(-1)),respectively, which is remarkably improved compared with that of continuous [Co/Pt]80multilayers. That is because large amounts of grain boundaries in the granular multilayers can efficiently impede the propagation and expansion of reversed magnetic domains, which is verified by experimental investigations and micromagnetic simulation results. The simulation results also indicate that the value of Mrt, Mr/Msratio, and Hcoopcan be further improved through optimizing the granule size, which can be experimentally realized by manipulating the process parameter of SiNxbuffer layer. This work provides an alternative solution for achieving high Mrt value in ultra-thick Co/Pt multilayers, which is of unneglectable potential in applications of high-density magnetic recording. 展开更多
关键词 granular Co/Pt multilayers perpendicular magnetic anisotropy remanent moment-thickness product magnetic recording
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Recording the direct surgeon’s view with an operating microscopic view improves microscopic ophthalmic surgery training
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作者 Masayuki Akimoto Kosei Tomita +1 位作者 Miyo Yoshida Yuki Hama 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第9期1555-1558,共4页
AIM:To establish a recording system with a direct view of the surgeon to supplement video recording under an operating microscope,which lacks information on the movement and position of the surgeon’s hands,and to fac... AIM:To establish a recording system with a direct view of the surgeon to supplement video recording under an operating microscope,which lacks information on the movement and position of the surgeon’s hands,and to facilitate the reproduction of a skilled surgeon’s technique by a surgeon in training.METHODS:A small camera was attached to the operating microscope with a custom adapter.Microscopic surgeon’s view and direct surgeon’s view through this new camera were recorded in the surgical recording system.Both movies were synchronized and analyzed how do surgeons handle the instruments.RESULTS:A small camera attached to the operating microscope allowed the surgeon’s hands motion to be recorded without interfering with the surgeon’s movements.Different surgeons used different methods to manipulate the ultrasound handpiece and the irrigation/aspiration device.Even in the simple paracentesis procedure,different surgeons used different methods.Surgeons-in-training were able to identify and improve their weaknesses by watching synchronized movies of their hand motions and microscopic view.CONCLUSION:Simultaneous recording the surgical field out of the operating microscopic view by a small camera set on the microscope is comprehensive and improves surgeons-in-training understanding and learning surgeries. 展开更多
关键词 operating microscopic view direct surgeon’s view video recording microscopic surgery training
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New record of the feather star Palaeocomatella hiwia(Echinodermata:Crinoidea:Comatulida)from Zhenbei Seamount in South China Sea:new insights into the taxonomic position of Palaeocomatella
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作者 Shao’e SUN Zijie MEI +2 位作者 Zhongli SHA Jing WANG Nansheng CHEN 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第1期244-251,共8页
One specimen belonging to the family Comatellinae was collected from the Zhenbei Seamount(332.5–478.2 m)in the South China Sea in July 2022.Based on the morphological characters,the specimen was identified as Palaeoc... One specimen belonging to the family Comatellinae was collected from the Zhenbei Seamount(332.5–478.2 m)in the South China Sea in July 2022.Based on the morphological characters,the specimen was identified as Palaeocomatella hiwia McKnight,1977.It is first recorded from China Sea and redescribed in detail.This specimen differs from the original description from New Zealand for never showing syzygy at br4+5 or br5+6 on interior and br1+2 on exterior arms.However,it is much conform to the redescription to specimens from Indonesia,with only differences in position of the second syzygy and distalmost pinnule comb.Specimen is deposited in the Institute of Oceanology,Chinese Academy of Sciences.Phylogenetic analyses based on the mitochondrial c oxidase subunit I(COI)and 16S rRNA genes indicated that P.hiwia was nested within the tribe Phanogeniini and clustered with Aphanocomaster pulcher.Furthermore,P.hiwia showed same morphological features in terms of mouth placement,comb location,and number of comb teeth rows as other genera of Phanogeniini.Therefore,we suggest that the genus Palaeocomatella should be put in the tribe Phanogeniini. 展开更多
关键词 new record Palaeocomatella hiwia McKnight 1977 South China Sea phylogenetic relationship TAXONOMY
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Impact of Laboratory Value Flowsheet in Electronic Health Record (EHR) Documentation Time
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作者 Isabel Rosado Pogozelski 《Open Journal of Nursing》 2024年第1期40-50,共11页
Research on the use of EHR is contradictory since it presents contradicting results regarding the time spent documenting. There is research that supports the use of electronic records as a tool to speed documentation;... Research on the use of EHR is contradictory since it presents contradicting results regarding the time spent documenting. There is research that supports the use of electronic records as a tool to speed documentation;and research that found that it is time consuming. The purpose of this quantitative retrospective before-after project was to measure the impact of using the laboratory value flowsheet within the EHR on documentation time. The research question was: “Does the use of a laboratory value flowsheet in the EHR impact documentation time by primary care providers (PCPs)?” The theoretical framework utilized in this project was the Donabedian Model. The population in this research was the two PCPs in a small primary care clinic in the northwest of Puerto Rico. The sample was composed of all the encounters during the months of October 2019 and December 2019. The data was obtained through data mining and analyzed using SPSS 27. The evaluative outcome of this project is that there is a decrease in documentation time after implementation of the use of the laboratory value flowsheet in the EHR. However, patients per day increase therefore having an impact on the number of patients seen per day/week/month. The implications for clinical practice include the use of templates to improve workflow and documentation as well as decreasing documentation time while also increasing the number of patients seen per day. . 展开更多
关键词 Electronic Health record EHR Laboratory Results Template Documentation Time
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The Development of the “Monitoring Application for Inappropriate Expressions in Nursing Records”for PsyNACS©
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作者 Hirokazu Ito Yuko Yasuhara Tetsuya Tanioka 《Journal of Computer and Communications》 2024年第5期42-52,共11页
Lengthening periods of hospitalization, increasing numbers of patients with age-related complications, and a shortage of nursing staff have been of great concern in medical psychiatry in Japan. Under these circumstanc... Lengthening periods of hospitalization, increasing numbers of patients with age-related complications, and a shortage of nursing staff have been of great concern in medical psychiatry in Japan. Under these circumstances, countries such as Japan that face a super-aging society and a decline in the working-age population, have been recommended for use of advanced information and communications technology (ICT) to improve the efficiency of medical treatment and care. This study aims to develop the “Monitoring Application for Inappropriate Expressions in Nursing Records” for using PsyNACS<sup>©</sup>, which will enable psychiatric nursing plans to harness the advantages of ICT. The functions considered necessary are as follows: 1) identification of users who enter information;2) a necessary database for lists of inappropriate expressions;3) development of a matching function to recommend proper writing input and a warning function;and 4) a management function for an inappropriate expression list database. A demonstration experiment for developing the application in this study was conducted at a specialized psychiatric hospital. To introduce them to the application, nurses and nurse managers were informed about the system developed in this study, and a survey regarding their opinions on the functions of the application was conducted with ten nurse managers. The results were evaluated in terms of usefulness for nursing care, documentation, and the education of nurses from an ethical perspective. This study suggests that matching their input with an inappropriate expression database will allow nurses to record more appropriate expressions. From an ethical perspective, the ability to use appropriate expressions makes records more likely to withstand disclosure requests from patients and their families. 展开更多
关键词 PsyNACS© Monitoring Application Inappropriate Expressions Nursing records
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解铃须从系铃处——Records与Archives概念正史(二)
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作者 王岚 《档案管理》 北大核心 2024年第1期28-35,共8页
笔者的“四正”,从名分、本义、学理、法律上已弄清Records的含义,但坚持“Records文件观”者依旧质疑:若Records是档案,那Archives是什么?两者并列时又怎么翻译?还有人说Records是记录。既然是英语和法语带来的问题,循着英法档案历史... 笔者的“四正”,从名分、本义、学理、法律上已弄清Records的含义,但坚持“Records文件观”者依旧质疑:若Records是档案,那Archives是什么?两者并列时又怎么翻译?还有人说Records是记录。既然是英语和法语带来的问题,循着英法档案历史去寻找,才是解铃的关键:以英法八百年档案工作中重要人物和事件为主线,探求其档案的源流、本质、内含及演变,为档案概念正史。 展开更多
关键词 卷档 录卷归档 档案 皇家档案 馆藏档案 现行档案 概念 历史
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FAULT LOCATION AND RECORDING SYSTEM OF TRANSMISSION LINES BASED ON COMPUTER NETWORK 被引量:1
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作者 贺家李 孙雅明 贺继红 《Transactions of Tianjin University》 EI CAS 1997年第1期17-23,共7页
提出以计算机网络为基础的超高压输电线路的故障定位和录波系统,在结构上具有开放式的特点,可根据要求方便地接入扩建线路,并且高速网络数通讯使系统具有极好的实时性.文中重点论述了故障定位新方法,它不仅在线路故障定碍位的数学... 提出以计算机网络为基础的超高压输电线路的故障定位和录波系统,在结构上具有开放式的特点,可根据要求方便地接入扩建线路,并且高速网络数通讯使系统具有极好的实时性.文中重点论述了故障定位新方法,它不仅在线路故障定碍位的数学模型建立上考虑了超高压长距离线路的分布参数特性,并且在计算方法上计及故障线路两端故障电流的相位,经EMTP仿真及测试可证明所提出的综合方法对超高运长距离输电线路故障定位的精确性.论述了以计算机网络为基础的输电线故障定位和录波系统,给出了系统结构和主要特点,重点阐述了所提出的精确超高压长距离输电线故障定位的新方法。 展开更多
关键词 计算机网络 故障定位 录波系统
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Strong motion observations and recordings from the great Wenchuan Earthquake 被引量:71
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作者 Li Xiaojun Zhou Zhenghua +5 位作者 Yu Haiyin Wen Ruizhi Lu Dawei Huang Moh Zhou Yongnian Cu Jianwen 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2008年第3期235-246,共12页
The National Strong Motion Observation Network System (NSMONS) of China is briefly introduced in this paper. The NSMONS consists of permanent free-field stations, special observation arrays, mobile observatories and... The National Strong Motion Observation Network System (NSMONS) of China is briefly introduced in this paper. The NSMONS consists of permanent free-field stations, special observation arrays, mobile observatories and a network management system. During the Wenchuan Earthquake, over 1,400 components of acceleration records were obtained from 460 permanent free-field stations and three arrays for topographical effect and structural response observation in the network system from the main shock, and over 20,000 components of acceleration records from strong aftershocks occurred before August 1, 2008 were also obtained by permanent free-field stations of the NSMONS and 59 mobile instruments quickly deployed after the main shock. The strong motion recordings from the main shock and strong aftershocks are summarized in this paper. In the ground motion recordings, there are over 560 components with peak ground acceleration (PGA) over 10 Gal, the largest being 957.7 Gal. The largest PGA recorded during the aftershock exceeds 300 Gal. 展开更多
关键词 Wenchuan Earthquake strong motion observation network mobile observation observation array strong motion record PGA
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Improved HVSR site classification method for free-field strong motion stations validated with Wenchuan aftershock recordings 被引量:14
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作者 Wen Ruizhi Ren Yefei Shi Dacheng 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2011年第3期325-337,共13页
Local site conditions play an important role in the effective application of strong motion recordings.In the China National Strong Motion Observation Network System(NSMONS),some of the stations do not provide boreho... Local site conditions play an important role in the effective application of strong motion recordings.In the China National Strong Motion Observation Network System(NSMONS),some of the stations do not provide borehole information,and correspondingly,do not assign the site classes yet.In this paper,site classification methodologies for free-field strong motion stations are reviewed and the limitations and uncertainties of the horizontal-to-vertical spectral ratio(HVSR) methods are discussed.Then,a new method for site classification based on the entropy weight theory is proposed.The proposed method avoids the head or tail joggle phenomenon by providing the objective and subjective weights.The method was applied to aftershock recordings from the 2008 Wenchuan earthquake,and 54 free-field NSMONS stations were selected for site classification and the mean HVSRs were calculated.The results show that the improved HVSR method proposed in this paper has a higher success rate and could be adopted in NSMONS. 展开更多
关键词 site classification strong motion recording entropy weight theory horizontal-to-vertical spectrum ratio Wenchuan earthquake aftershock head-tail joggle
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Magnetic property and recording performance of chemical deposition CoP thin films 被引量:3
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作者 Wang, Haicheng Zhou, Qunfei +2 位作者 Teng, Jiao Wang, Lijin Yu, Guanghua 《Rare Metals》 SCIE EI CAS CSCD 2012年第3期260-263,共4页
关键词 COP thin films magnetic property magnetic recording
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Characteristics of response spectra for long-periods of main-shock recordings of the Chi-Chi earthquake 被引量:3
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作者 陈勇 俞言祥 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2007年第2期111-121,共11页
Current practice uses predictive models to extrapolate long-period response spectra based on far-field recordings in moderate and weak earthquakes. However, the spectra are not long enough and the data are often not r... Current practice uses predictive models to extrapolate long-period response spectra based on far-field recordings in moderate and weak earthquakes. However, the spectra are not long enough and the data are often not reliable, which means that the seismic design code cannot accurately define seismic design requirements for long-period structures. The near-field recordings in the main-shock of the Chi-Chi earthquake have a large signal-to-noise ratio (SNR), which makes them suitable for studying the long-period acceleration response spectrum up to 20 sec. The acceleration response spectra from 246 stations within 120 km of the causative fault are statistically analyzed in this paper. The influence of distance and site conditions on long-period response spectrum is discussed, and the shapes of the amplification spectra are compared with the standard spectra specified in the seismic design code of China. Finally, suggestions for future revisions to the code are proposed. 展开更多
关键词 long-period main-shock recordings Chi-Chi earthquake signal-to-noise ratio acceleration response spectrum amplification spectrum
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Multilevel read-only optical recording methods 被引量:2
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作者 宋洁 徐端颐 +3 位作者 齐国生 胡华 张启程 熊剑平 《Chinese Physics B》 SCIE EI CAS CSCD 2006年第8期1788-1792,共5页
The advantages of read-only storage is the predominance of optical recording relative to magnetic and other rewritable methods. Multilevel (ML) read-only technology has been a trend to improve the data capacity and ... The advantages of read-only storage is the predominance of optical recording relative to magnetic and other rewritable methods. Multilevel (ML) read-only technology has been a trend to improve the data capacity and transfer rate. Based on the principle and coding method of ML, this paper demonstrates some ML read-only recording methods, of which a new ML read-only recording is developed. This recording method integrates amplitude modulation achieved by the reaction mechanism of physics and chemistry of photoresist with the run-length-limited technology. The discs can be achieved using standard photoresist mastering and replication techniques with great compatibility to conventional binary read-only discs. 展开更多
关键词 multilevel optical recording read-only amplitude modulation
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The 2018 M_S 5.9 Mojiang Earthquake:Source model and intensity based on near-field seismic recordings 被引量:4
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作者 Xu Zhang Zhen Fu +2 位作者 LiSheng Xu ChunLai Li Hong Fu 《Earth and Planetary Physics》 CSCD 2019年第3期268-281,共14页
On September 8, 2018, an M_S 5.9 earthquake struck Mojiang, a county in Yunnan Province, China. We collect near-field seismic recordings(epicentral distances less than 200 km) to relocate the mainshock and the aftersh... On September 8, 2018, an M_S 5.9 earthquake struck Mojiang, a county in Yunnan Province, China. We collect near-field seismic recordings(epicentral distances less than 200 km) to relocate the mainshock and the aftershocks within the first 60 hours to determine the focal mechanism solutions of the mainshock and some of the aftershocks and to invert for the finite-fault model of the mainshock.The focal mechanism solution of the mainshock and the relocation results of the aftershocks constrain the mainshock on a nearly vertical fault plane striking northeast and dipping to the southeast. The inversion of the finite-fault model reveals only a single slip asperity on the fault plane. The major slip is distributed above the initiation point, ~14 km wide along the down-dip direction and ~14 km long along the strike direction, with a maximal slip of ~22 cm at a depth of ~6 km. The focal mechanism solutions of the aftershocks show that most of the aftershocks are of the strike-slip type, a number of them are of the normal-slip type, and only a few of them are of the thrust-slip type.On average, strike-slip is dominant on the fault plane of the mainshock, as the focal mechanism solution of the mainshock suggests, but when examined in detail, slight thrust-slip appears on the southwest of the fault plane while an obvious part of normal-slip appears on the northeast, which is consistent with what the focal mechanism solutions of the aftershocks display. The multiple types of aftershock focal mechanism solutions and the slip details of the mainshock both suggest a complex tectonic setting, stress setting, or both. The intensity contours predicted exhibit a longer axis trending from northeast to southwest and a maximal intensity of Ⅷ around the epicenter and in the northwest. 展开更多
关键词 2018 MS 5.9 Mojiang EARTHQUAKE NEAR-FIELD SEISMIC recording finite-fault model INTENSITY prediction FOCAL mechanism solution
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An Effective Method to Determine the Distribution Boundary of Cobalt-Rich Fe-Mn Crusts on a Guyot: Synchronous Application of Sub-bottom Profiling and Deep-Sea Video Recording 被引量:3
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作者 HeGaowen LiangDonghong +4 位作者 SongChengbin SunXiaoming WuShuigen ZhouJianping ZhangXuehua 《Journal of China University of Geosciences》 SCIE CSCD 2005年第2期178-182,共5页
关键词 sub-bottom profiling deep-sea video recording guyot cobalt-rich ferromanganese crust distribution boundary
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Micromagnetic studies of perpendicular recording FePt media with controllable grain size distributions 被引量:1
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作者 DONG Zhong PIAO Kun SHE Shengxian WEI Dan LI Zhenghua WEI Fulin 《Rare Metals》 SCIE EI CAS CSCD 2006年第z1期436-439,共4页
A 3-dimensional (3D) micromagnetic model combined with Fast Fourier Transform (FFT) method was built up to study the writability in the L10 FePt perpendicular medium. The effects of controllable grain size distributio... A 3-dimensional (3D) micromagnetic model combined with Fast Fourier Transform (FFT) method was built up to study the writability in the L10 FePt perpendicular medium. The effects of controllable grain size distributions were studied by grain growth simulation. It is found that the cross-track-averaged magnetization changes little between the L10 FePt medium with uniform or non-uniform grain size distribution. 展开更多
关键词 PERPENDICULAR recording L10 FEPT medium CONTROLLABLE grain size DISTRIBUTIONS MICROMAGNETIC simulation
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Scattering-Suppression in Photochromic LiNbO_(3):Fe:Mn Non-volatile Holographic Recording 被引量:2
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作者 刘德安 刘立人 +2 位作者 刘友文 周常河 徐良瑛 《Chinese Physics Letters》 SCIE CAS CSCD 2001年第8期1064-1066,共3页
We propose and experimentally investigate a new scheme capable of suppressing the light-induced scattering by periodical incoherent erasure during every non-volatile holographic recording cycle in photochromic LiNbO_(... We propose and experimentally investigate a new scheme capable of suppressing the light-induced scattering by periodical incoherent erasure during every non-volatile holographic recording cycle in photochromic LiNbO_(3):Fe:Mn crystals.The results demonstrate that the scattering noise is suppressed effectively,and the final diffraction efficiency of the fixed grating is significantly enhanced,rather than decrease,by about 30%compared with the conventional recording procedure.The period of the recording and incoherent erasure cycle is theoretically calculated and experimentally optimized. 展开更多
关键词 recording VOLATILE CYCLE
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In vivo patch clamp recording technique in the study of neurophysiology 被引量:1
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作者 Lan-Wei CUI Yu-Rong LI 《Neuroscience Bulletin》 SCIE CAS CSCD 2006年第1期68-71,共4页
The patch clamp recording technique in vivois a blind patch clamp recording methods to record the current of the spinal or cereral neurons of anaes:hesia ( or awake) animals. This technique can be used to study the... The patch clamp recording technique in vivois a blind patch clamp recording methods to record the current of the spinal or cereral neurons of anaes:hesia ( or awake) animals. This technique can be used to study the synaptic function and plasticity in central nervous system in vivoin order to understand the physiological properties of the ion channels from an integrated point of view. The advantage of this technique have already presented itself in the study of the synaptic transmission and nervous network. Nowadays, in vivo patch whole-cell recording technique in combination with other techniques is becoming a common method in the research fields. 展开更多
关键词 patch clamp recording technique in vivo PHYSIOLOGY
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Magnetic property and microstructure of SmCo magnetic recording films 被引量:1
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作者 李宁 李帅 +1 位作者 张恩 王立锦 《Journal of Rare Earths》 SCIE EI CAS CSCD 2009年第5期839-842,共4页
Cr/SmCo/Cr thin films with Sm concentration of 37.7 at.% were deposited on glass substrates by magnetron sputtering. Measurement of magnetic properties showed that the SmCo film possessed good magnetic anisotropy, a h... Cr/SmCo/Cr thin films with Sm concentration of 37.7 at.% were deposited on glass substrates by magnetron sputtering. Measurement of magnetic properties showed that the SmCo film possessed good magnetic anisotropy, a high coercivity of 3019 kA/m and low magnetic exchange coupling. Microstructure analysis showed that crystallized SmCo5 magnetic phase, non-magnetic SmCo2 phase and Sm2Co7 phase co-existed in the film. The non-magnetic SmCo2 phase might function as isolator of SmCo grains, leading to a decrease of magnetic exchange coupling. Moreover, a Cr2O3 oxide layer which could protect the SmCo layer from oxidation formed at the surface of the Cr cap layer. 展开更多
关键词 SmCo thin film magnetic recording magnetic exchange coupling rare earths
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Whole-cell recording of the robust nucleus of the arcopallium neurons in the adult zebra finch 被引量:1
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作者 Suqun Liao Xiaolin Liu Dongfeng Li 《Neural Regeneration Research》 SCIE CAS CSCD 2009年第8期623-628,共6页
BACKGROUND: Electrophysiological properties of the song nucleus have been revealed using conventional techniques, such as intracellular and extracellular recording. Research concerning the neuronal activation propert... BACKGROUND: Electrophysiological properties of the song nucleus have been revealed using conventional techniques, such as intracellular and extracellular recording. Research concerning the neuronal activation properties and regulations of the song system at the cellular and ion channel level may help reveal the neural mechanism of song learning. OBJECTIVE: To perform whole-cell recording of robust nucleus of the arcopallium (RA) neurons in brain slices from adult zebra finches (Taeniopygia guttata) and observe the action potential, sodium/potassium current and the spontaneous postsynaptic current of RA neurons. DESIGN, TIME AND SETTING: Self-controlled, neuroelectrophysiological experiment. The study was performed at the Neurophysiology Laboratory of South China Normal University from April to September 2008. MATERIALS: Flaming/Brown puller P-97 was purchased from Sutter Ins, USA; Axopatch 700B amplifier and Digidata 1332A converter were purchased from Axon Instrument, USA; pClamp software was provided by Axon Instrument, USA. METHODS: RA neurons were acutely isolated from 24 healthy male zebra finches. The action potential, voltage-gate sodium/potassium current and spontaneous postsynaptic current were recorded by whole-cell recording technology. Data were analyzed by pClamp software. MAIN OUTCOME MEASURES: The amplitude and frequency of the action potential, and the amplitude of the voltage-dependent and spontaneous postsynaptic currents, were measured. RESULTS: (1) Testing of action potential: Cells exhibited a stable current-voltage relationship following a series of hyperpolarization stepped currents, and an action potential was triggered by the spike threshold. All the recorded cells displayed repetitive firing following depolarizing current injection, with a frequency beyond 100 Hz. (2) Testing of voltage-gate currents: The inward and outward whole-cell currents were observed after a series of depolarizing voltage steps. The inward current disappeared following the application of tetrodotoxin and the outward current was significantly inhibited by application of 4-aminopyfidione and tetraethylammonium chloride. (3) Testing of spontaneous postsynaptic current: The majority of recorded cells exhibited an inward synaptic current when the membrane potential was maintained at -60 mV, with some cells exhibiting a robustly outward current when the membrane potential was maintained at -30 mV. Tetrodotoxin was unable to affect the spontaneous postsynaptic current. Following application of bicuculline [y-aminobutyric acid (A) receptor antagonist] and high concentration kynurenic acid (ionotropic glutamate receptor antagonist), the inward and outward currents were completely inhibited. CONCLUSION: Under these experimental conditions, the action potential, sodium/potassium current and spontaneous postsynaptic current were recorded successfully in RA neurons. This indicates that the cells preserved relatively intact synaptic connections and normal physiological activity, which is required for investigating ion channels. The inward and outward whole-cell currents were sodium and potassium currents, respectively. The postsynaptic y-aminobutyric acid (A) receptors and ionotropic glutamate receptors contributed to the spontaneous postsynaptic current. 展开更多
关键词 adult zebra finch robust nucleus of the arcopallium whole-cell recording
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Improvement of Mechanical Stability for Single Unit Recording Based on Skull Cap in Living Chinchilla 被引量:2
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作者 Peng-yu REN Bo-wen +2 位作者 Shi-yao DONG Ming WU Peng HAN 《Current Medical Science》 SCIE CAS 2019年第1期166-172,共7页
Three-point head fixation was constructed to provide mechanical stability for single unit recording(SUR)on vestibular sensory system in living chinchilla previously.However,it is no more qualified to this work when th... Three-point head fixation was constructed to provide mechanical stability for single unit recording(SUR)on vestibular sensory system in living chinchilla previously.However,it is no more qualified to this work when the stimulation intensity becomes large because of frequent unit losing and neuron damage,which strongly implies that the mechanical stability has been broken during the stimulation.Here,we constructed a novel hea0 fixation(skull cap assistant head fixation)provided by skull cap on the basis of three-point head fixation in order to improve the mechanical stability for SUR under the stimulation with large magnitude.The large area bone connection is the feature and advantage of this improved method,which directly fixes the tested local nervous tissue and microelectrode in an intact stable system through skull cap except two ear bars and a tube face mask.Our data exhibited that skull cap assistant head fixation could significantly improve the success rate of neural response activity recording in the population of semicircular canal neurons under the stimulation with large intensity(amplitudd 00 deg/s).Based on the analysis of neural response activity and noise base-line during stimulation,our data further indicated that this method could significantly improve the mechanical stability for SUR during high-speed motion stimulation on vestibular system in living chinchilla.Skull cap assistant head fixation extends the application of SUR on vestibular neuron in linear response range and provides a solid foundation for electrophysiological research on vestibular sensory system in fhrther studies. 展开更多
关键词 single unit recording SKULL CAP mechanical stability IMPROVEMENT semicircular CANAL NEURONS
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