目的评估Autof MS 1000质谱鉴定系统对临床实验室常见菌株的鉴定能力。方法参考CLSI M52标准对Autof MS 1000质谱鉴定系统进行评估。菌株鉴定的评估指标包括准确度和精密度。准确度评估以Bruker MALDI-TOF作为参比设备,以Autof MS 1000...目的评估Autof MS 1000质谱鉴定系统对临床实验室常见菌株的鉴定能力。方法参考CLSI M52标准对Autof MS 1000质谱鉴定系统进行评估。菌株鉴定的评估指标包括准确度和精密度。准确度评估以Bruker MALDI-TOF作为参比设备,以Autof MS 1000为待评估设备,选取231株能够涵盖实验室80%~90%的常见分离菌株及1株室间质评菌株作为检测标本,包括革兰氏阳性需氧菌77株,革兰氏阴性需氧菌95株,厌氧菌30株,酵母样真菌30株,待评估设备鉴定结果与参比设备鉴定结果一致时判断为相符,若结果不一致则以测序方法(细菌为16S RNA,真菌为ITS区测序)鉴定结果为准。精密度评估为选取12株室内质控菌株和临床分离菌株作为检测标本,在Autof MS 1000质谱鉴定系统上进行重复性测试,每株菌重复检测3次,判断其鉴定结果的一致性。以准确度大于90%、精密度大于95%为判断合格标准。结果232株临床实验室常见分离菌株鉴定结果准确度为100%;12株室内质控菌株和临床分离菌株重复性检测结果的精密度为100%。结论Autof MS 1000质谱鉴定系统对临床实验室常见菌株鉴定的准确度和精密度都很高,并且具有快速、低耗及易操作等优势,建议可在临床微生物实验室推广使用。展开更多
Linux has a special feature of automount in the Linux kernel. Filesystem can be mounted and unmounted automatically. Its performance is similar to MS Windows. It works well under demands of more saving resources and a...Linux has a special feature of automount in the Linux kernel. Filesystem can be mounted and unmounted automatically. Its performance is similar to MS Windows. It works well under demands of more saving resources and automatization, like memory of system. An approach to install/update software automatically on embedded platform was proposed. This approach derives from above feature based on embedded environment with Linux OS. Configurations of files related to this feature were introduced. An example in practice was given to realize this approach from universal serial bus (USB) memory disk when USB disk is available.展开更多
文摘目的评估Autof MS 1000质谱鉴定系统对临床实验室常见菌株的鉴定能力。方法参考CLSI M52标准对Autof MS 1000质谱鉴定系统进行评估。菌株鉴定的评估指标包括准确度和精密度。准确度评估以Bruker MALDI-TOF作为参比设备,以Autof MS 1000为待评估设备,选取231株能够涵盖实验室80%~90%的常见分离菌株及1株室间质评菌株作为检测标本,包括革兰氏阳性需氧菌77株,革兰氏阴性需氧菌95株,厌氧菌30株,酵母样真菌30株,待评估设备鉴定结果与参比设备鉴定结果一致时判断为相符,若结果不一致则以测序方法(细菌为16S RNA,真菌为ITS区测序)鉴定结果为准。精密度评估为选取12株室内质控菌株和临床分离菌株作为检测标本,在Autof MS 1000质谱鉴定系统上进行重复性测试,每株菌重复检测3次,判断其鉴定结果的一致性。以准确度大于90%、精密度大于95%为判断合格标准。结果232株临床实验室常见分离菌株鉴定结果准确度为100%;12株室内质控菌株和临床分离菌株重复性检测结果的精密度为100%。结论Autof MS 1000质谱鉴定系统对临床实验室常见菌株鉴定的准确度和精密度都很高,并且具有快速、低耗及易操作等优势,建议可在临床微生物实验室推广使用。
文摘Linux has a special feature of automount in the Linux kernel. Filesystem can be mounted and unmounted automatically. Its performance is similar to MS Windows. It works well under demands of more saving resources and automatization, like memory of system. An approach to install/update software automatically on embedded platform was proposed. This approach derives from above feature based on embedded environment with Linux OS. Configurations of files related to this feature were introduced. An example in practice was given to realize this approach from universal serial bus (USB) memory disk when USB disk is available.
